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11. 发明授权

US6165723A Insitu hybridization method for detecting target nucleic acid 有权
标题翻译：用于检测靶核酸的杂交方法
公开(公告)号：US6165723A
公开(公告)日：2000-12-26
申请号：US313852
申请日：1999-05-18
申请人： Jyotsna S. Shah , Nick S. Harris
发明人： Jyotsna S. Shah , Nick S. Harris
IPC分类号： C12Q1/68 , C07H21/04 , G01N1/30
CPC分类号： C12Q1/6841 , C12Q1/6888 , C12Q1/6893 , C12Q2527/125 , C12Q2563/107
摘要： The present invention provides a method for detecting a target nucleic acid fragment directly from a specimen obtained from a patient by in situ hybridization. The method is comprised of several steps which are performed in the listed order. A sample of the specimen is deposited onto a slide. The sample is fixed onto the slide with fixative, the fixative comprising either methanol-acetic acid at a ratio of from 99:1 to 80:20, or formalin-acetic acid at a ratio of from 99:1 to 80:20. The nucleic acids of the fixed sample are contacted with a probe complex specific for the target nucleic acid fragment, under conditions appropriate for hybridization. Non-hybridized probe complex is rinsed from the sample. The rinsed sample is stained with Evans Blue. The hybridized probe complex is visually detected by microscopy, with the presence of the probe complex being an indication of the presence of the target nucleic acid fragment. The method can be performed with different hybridization buffers, several of which are disclosed. The method of the present invention is useful for detecting pathogens in a specimen. Specific probe complexes are disclosed which are useful for detecting pathogens of the species Babesia. The method is useful in detecting nucleic acids from a wide variety of specimens, including serum, plasma, sputum, urine, cerebral spinal fluids, tissue, breast milk, and insects such as ticks.
摘要翻译：本发明提供了通过原位杂交直接从患者获得的标本中检测靶核酸片段的方法。该方法由按照列出的顺序执行的几个步骤组成。将试样的样品沉积在载玻片上。将样品用固定剂固定在载玻片上，固定剂包含比例为99:1至80:20的甲醇 - 乙酸或比例为99:1至80:20的福尔马林 - 乙酸。将固定样品的核酸与靶核酸片段特异性的探针复合物在适于杂交的条件下接触。从样品中冲洗非杂交探针复合物。冲洗的样品用伊文思蓝染色。通过显微镜目视检测杂交的探针复合物，探针复合物的存在是靶核酸片段的存在的指示。该方法可以用不同的杂交缓冲液进行，其中几种是公开的。本发明的方法可用于检测样品中的病原体。公开了特异性探针复合物，其可用于检测物种Babesia的病原体。该方法可用于检测各种标本中的核酸，包括血清，血浆，痰液，尿液，脑脊髓液，组织，母乳和昆虫如蜱。

12. 发明授权

US5593831A Nucleic acid probes for the detection of yersinia enterolitica 失效
标题翻译：用于检测肠球菌的核酸探针
公开(公告)号：US5593831A
公开(公告)日：1997-01-14
申请号：US297103
申请日：1994-08-29
申请人： Jyotsna S. Shah , Samuel W. Chan , Theodore B. Pitman , David J. Lane
发明人： Jyotsna S. Shah , Samuel W. Chan , Theodore B. Pitman , David J. Lane
IPC分类号： C07H21/00 , C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , C12Q1/689 , Y10S435/81
摘要： Nucleic acid probes capable of specifically hybridizing to rRNA of Yersinia enterocolitica and not to rRNA of non-Yersinia enterocolitica are described along with methods utilizing such probes for the detection of Yersinia enterocolitica in food and other samples.
摘要翻译：与能够与小肠结肠炎耶尔森氏菌的rRNA特异性杂交的核酸探针与不是小肠结肠炎耶尔森氏菌的rRNA一起描述，以及利用这种探针在食物和其它样品中检测小肠结肠耶路西亚的方法。

13. 发明授权

US5521300A Oligonucleotides complementary to mycobacterial nucleic acids 失效
标题翻译：与分枝杆菌核酸互补的寡核苷酸
公开(公告)号：US5521300A
公开(公告)日：1996-05-28
申请号：US744282
申请日：1991-08-13
申请人： Jyotsna S. Shah , Ray M. Nietupski , Jing Liu
发明人： Jyotsna S. Shah , Ray M. Nietupski , Jing Liu
IPC分类号： C12N15/09 , C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/689
摘要： Disclosed are nucleic acid oligonucleotides which are substantially complementary to nucleic acids from Mycobacteria, and subgeneric classes thereof. More specifically, the oligonucleotides are complementary to ribosomal RNA (rRNA) and the DNA encoding rRNA (rDNA). Uses for such oligonucleotides include detection of Mycobacteria by hybridization and amplification of Mycobacterial nucleic acid by polymerase chain reaction.
摘要翻译：公开了与分枝杆菌的核酸基本互补的核酸寡核苷酸及其亚类。更具体地，寡核苷酸与核糖体RNA（rRNA）和编码rRNA（rDNA）的DNA互补。用于这样的寡核苷酸包括通过聚合酶链反应杂交和扩增分枝杆菌核酸来检测分枝杆菌。

14. 发明授权

US5376528A Probes and methods for the detection of Listeria 失效
标题翻译：用于检测李斯特菌的探针和方法
公开(公告)号：US5376528A
公开(公告)日：1994-12-27
申请号：US020241
申请日：1993-02-19
申请人： Walter King , Jyotsna S. Shah , Raymond M. Nietupski , Susan Raposa , Jane Warshaw , Patrick Groody , Jonathan Lawrie , George Parsons , Donald N. Halbert , David J. Lane
发明人： Walter King , Jyotsna S. Shah , Raymond M. Nietupski , Susan Raposa , Jane Warshaw , Patrick Groody , Jonathan Lawrie , George Parsons , Donald N. Halbert , David J. Lane
IPC分类号： C12Q1/04 , C12Q1/68 , C12R1/01 , C07H21/04
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/689
摘要： Improved nucleic acid probes capable of specifically hybridizing to rRNA of Listeria and not to rRNA of non-Listeria are described along with methods utilizing such probes for the detection of Listeria in food and other samples.
摘要翻译：与利斯特氏菌的rRNA特异性杂交而不是非李斯特菌的rRNA的改良的核酸探针被描述，同时利用这种探针来检测食物和其他样品中的李斯特菌。

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