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11. 发明授权

US09034278B2 Sample preparation for spectroscopy analysis 有权
标题翻译：光谱分析样品制备
公开(公告)号：US09034278B2
公开(公告)日：2015-05-19
申请号：US13597418
申请日：2012-08-29
申请人： Andrew P. Bartko , Johnway Gao
发明人： Andrew P. Bartko , Johnway Gao
IPC分类号： A61B10/00 , G01N35/00 , G01N1/31
CPC分类号： G01N1/30 , G01N1/312 , G01N35/00029 , Y10T436/2575
摘要： A sample is cleaned up for spectroscopic analysis by receiving a slide substrate having the sample thereon, fixing the sample to a substrate surface of the slide substrate by applying heat to the slide substrate for a predetermined heating time and incubating the sample on the slide substrate for a predetermined incubation time after fixing the sample to the slide substrate. The sample is further cleaned by washing the sample on the slide substrate after the sample has been incubated and drying the sample by applying heat to the slide substrate for a predetermined drying time, wherein the sample on the slide substrate after drying has retained particles of interest and interferant particles are removed from the substrate. A substrate is also provided for sample collection, which is culturable and Raman silent.
摘要翻译：通过接收具有样品的载玻片基板将样品清理干净，用于将样品固定在载玻片基板的基板表面上，通过向滑动基板施加预定加热时间并将样品温育在载玻片基板上在将样品固定到载玻片基板之后的预定孵育时间。通过在将样品温育并将样品通过向滑动基底施加热量预定的干燥时间来干燥样品之后，通过在载玻片基板上洗涤样品来进一步清洗样品，其中在干燥后的载玻片基板上的样品已经保留了感兴趣的颗粒并且从衬底去除干燥剂颗粒。还提供了用于样品收集的底物，其是可培养的和拉曼沉默的。

12. 发明授权

US06783978B1 Isolation and characterization of the genomic DNA clones of ribosomal protein gene L25 in tobacco 失效
标题翻译：烟草中核糖体蛋白基因L25的基因组DNA克隆的分离和表征
公开(公告)号：US06783978B1
公开(公告)日：2004-08-31
申请号：US09714948
申请日：2000-11-20
申请人： Lifang Shi , Johnway Gao , Brian S. Hooker , Ziyu Dai
发明人： Lifang Shi , Johnway Gao , Brian S. Hooker , Ziyu Dai
IPC分类号： C12N1500
CPC分类号： C12N15/8234 , C12N15/8225
摘要： The promoter of the ribosomal protein gene L25 is operably linked to a structural gene. This chimeric gene is placed in an expression vector. The expression vector containing the chimeric gene is used to transform plants cells and plants. Seeds are obtained from the transformed plants. A product, such as a protein, encoded by the structural gene is isolated from the transformed plant cells and plants.
摘要翻译：核糖体蛋白基因L25的启动子与结构基因可操作地连接。将该嵌合基因置于表达载体中。含有嵌合基因的表达载体用于转化植物细胞和植物。从转化的植物获得种子。从转化的植物细胞和植物中分离由结构基因编码的产物，如蛋白质。

13. 发明授权

US06355864B1 Versatile rpL34 promoter elements and use thereof 失效
标题翻译：多功能rpL34启动子元件及其用途
公开(公告)号：US06355864B1
公开(公告)日：2002-03-12
申请号：US09417019
申请日：1999-10-12
申请人： Lifang Shi , Ziyu Dai , Johnway Gao , Brian S. Hooker , Daniel B. Anderson
发明人： Lifang Shi , Ziyu Dai , Johnway Gao , Brian S. Hooker , Daniel B. Anderson
IPC分类号： C07H2104
CPC分类号： C12N15/8238 , C12N15/8216 , C12N15/8229 , C12N15/8237 , C12N15/8239
摘要： Synergistically functional, yet separable, cis-acting enhancer elements from the rpL34 promoter are disclosed. These enhancer elements of the instant invention may be used in combination with a plurality of promoters to increase gene expression without affecting the intrinsic specificity of the promoters. Also disclosed are methods for using the enhancer elements of the instant invention as well as vectors and transgenic plants comprising the enhancer elements.
摘要翻译：公开了来自rpL34启动子的协同作用的，但可分离的顺式作用的增强子元件。本发明的这些增强子元件可以与多个启动子组合使用以增加基因表达而不影响启动子的固有特异性。还公开了使用本发明的增强子元件以及包含增强子元件的载体和转基因植物的方法。

14. 发明申请

US20130118483A1 SOLID LIGNOCELLULOSIC HYDROLYSATE AND METHODS TO PREPARE A SOLID LIGNOCELLULOSIC HYDROLYSATE 审中-公开
标题翻译：固体水杨酸水解产物及其制备方法制备固体水杨酸
公开(公告)号：US20130118483A1
公开(公告)日：2013-05-16
申请号：US13294867
申请日：2011-11-11
申请人： Johnway Gao , Dwight Anderson , Benjamin Levie
发明人： Johnway Gao , Dwight Anderson , Benjamin Levie
IPC分类号： C13B50/00 , C13K1/02
CPC分类号： C13K1/04 , C12P7/10 , C12P19/14 , C13K13/007 , D21C1/04 , D21C3/00 , D21C5/005 , Y02E50/16 , Y02E50/343
摘要： The present disclosure provides a solid lignocellulosic hydrolysate and methods to prepare the solid lignocellulosic hydrolysate from a woody biomass or an herbaceous biomass. The solid lignocellulosic hydrolysate may be used in the production of biofuels, bioproducts, and food products. The solid lignocellulosic hydrolysate allows for ease of storage, ease of transportation and handling of the solid lignocellulosic hydrolysate, and ease of use in biological or fermentation processes or chemical processes for the production of biofuel, bioproducts, chemicals and food products due to the bulk handling characteristics (e.g., solubility and rate of dissolution) of the solid lignocellulosic hydrolysate.
摘要翻译：本公开提供了一种固体木质纤维素水解物和从木质生物质或草本生物质制备固体木质纤维素水解产物的方法。固体木质纤维素水解产物可用于生产生物燃料，生物产品和食品。固体木质纤维素水解产物容易储存，易于运输和处理固体木质纤维素水解产物，并且易于在生物或发酵过程中使用或由于大批量处理而用于生产生物燃料，生物产品，化学品和食品的化学方法固体木质纤维素水解产物的特征（例如溶解度和溶解速率）。

15. 发明申请

US20110124056A1 Pretreatment of Ligno-Cellulosic Biomass with Sulfonation 审中-公开
标题翻译：用磺化预处理木质纤维素生物质
公开(公告)号：US20110124056A1
公开(公告)日：2011-05-26
申请号：US12894042
申请日：2010-09-29
申请人： Benjamin Levie , Rajesh Gupta , Johnway Gao , Dwight Anderson
发明人： Benjamin Levie , Rajesh Gupta , Johnway Gao , Dwight Anderson
IPC分类号： C12P19/04 , C12P19/02 , C13K1/02
CPC分类号： C13K1/02 , C12P19/02 , C12P19/14 , C12P2201/00 , D21C3/06 , D21C11/0007
摘要： Provided are methods for the pretreatment of ligno-cellulosic biomass such as softwoods with bisulfite such as ammonium bisulfite without the need for exogenous acid. In one variation, a method of pretreating ligno-cellulosic biomass is provided including the following steps: a) providing ligno-cellulosic biomass; b) contacting the ligno-cellulosic biomass with a solution comprising bisulfite at an amount between 1 and 10% of a dry weight of the ligno-cellulosic biomass to form a slurry; c) heating the slurry to a first temperature of 150-210° C. for a first period of time to form a first mixture; d) cooling the first mixture to a second temperature of 100-200° C. to form a second mixture; and e) maintaining the second mixture at the second temperature for a second period of time to form pretreated ligno-cellulosic biomass; wherein the first temperature is higher than the second temperature.
摘要翻译：提供了用于预处理木质纤维素生物质的方法，例如具有亚硫酸氢盐的软木，例如亚硫酸氢铵，而不需要外源性酸。在一个实施方案中，提供了一种预处理木质纤维素生物质的方法，包括以下步骤：a）提供木质纤维素生物质; b）使木质纤维素生物质与包含亚硫酸氢盐的溶液接触，其量为木质纤维素生物质的干重的1至10％，以形成浆料; c）将所述浆料加热至第一温度为150-210℃，持续第一时间以形成第一混合物; d）将第一混合物冷却至100-200℃的第二温度以形成第二混合物; 和e）将所述第二混合物保持在所述第二温度第二时间段以形成预处理的木质纤维素生物质; 其中所述第一温度高于所述第二温度。

16. 发明授权

US06551798B2 Method for using a yeast alpha-amylase promoter 失效
标题翻译：使用酵母α-淀粉酶启动子的方法
公开(公告)号：US06551798B2
公开(公告)日：2003-04-22
申请号：US09921942
申请日：2001-08-02
申请人： Johnway Gao , Rodney S. Skeen , Brian S. Hooker , Daniel B. Anderson
发明人： Johnway Gao , Rodney S. Skeen , Brian S. Hooker , Daniel B. Anderson
IPC分类号： C12N1563
CPC分类号： C12N9/242 , C12N15/81
摘要： The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.
摘要翻译：本发明提供了使用酵母菌株施氏葡萄球菌（Schwanniomyces castellii）的淀粉的α-淀粉酶基因的启动子克隆发现。分离的α-淀粉酶启动子是可诱导的启动子，可以调节淀粉培养基中的强基因表达。

17. 发明授权

US06395175B1 Method and apparatus for energy efficient self-aeration in chemical, biochemical, and wastewater treatment processes 失效
标题翻译：化学，生化和废水处理过程中能量效率自曝气的方法和装置
公开(公告)号：US06395175B1
公开(公告)日：2002-05-28
申请号：US09541868
申请日：2000-04-03
申请人： Johnway Gao , Rodney S. Skeen
发明人： Johnway Gao , Rodney S. Skeen
IPC分类号： C02F174
CPC分类号： B01F3/0446 , B01F5/0646 , B01F5/0655 , B01F5/0659 , B01F2215/0052 , C02F1/74 , C02F2301/063 , Y10S261/54 , Y10S261/75
摘要： The present invention is a pulse spilling self-aerator (PSSA) that has the potential to greatly lower the installation, operation, and maintenance cost associated with aerating and mixing aqueous solutions. Currently, large quantities of low-pressure air are required in aeration systems to support many biochemical production processes and wastewater treatment plants. Oxygen is traditionally supplied and mixed by a compressor or blower and a mechanical agitator. These systems have high-energy requirements and high installation and maintenance costs. The PSSA provides a mixing and aeration capability that can increase operational efficiency and reduce overall cost.
摘要翻译：本发明是一种脉冲溢出自曝气（PSSA），其具有大大降低与曝气和混合水溶液相关的安装，操作和维护成本的潜力。目前，曝气系统需要大量的低压空气来支持许多生化生产过程和污水处理厂。氧气传统上由压缩机或鼓风机和机械搅拌器供应和混合。这些系统具有高能量要求和高安装和维护成本。 PSSA提供混合和曝气功能，可以提高运营效率并降低总体成本。

18. 发明申请

US20120264178A1 METHODS OF ENABLING ENZYMATIC HYDROLYSIS AND FERMENTATION OF LIGNOCELLULOSIC BIOMASS WITH PRETREATED FEEDSTOCK FOLLOWING HIGH SOLIDS STORAGE IN THE PRESENCE OF ENZYMES 审中-公开
标题翻译：在酶的存在下，利用预处理饲料进行酶促水解和发酵的低密度生物质的方法
公开(公告)号：US20120264178A1
公开(公告)日：2012-10-18
申请号：US13416911
申请日：2012-03-09
申请人： Dwight ANDERSON , Johnway Gao , Benjamin Levie
发明人： Dwight ANDERSON , Johnway Gao , Benjamin Levie
IPC分类号： C12P19/02 , C12P19/00
CPC分类号： C12P7/10 , C12P19/00 , C12P19/02 , C12P19/14 , C12P2201/00 , Y02E50/16
摘要： The present invention provides methods of producing pretreated lignocellulosic biomass combined with enzymes for the storage and transporation of the pretreated lignocellulosic biomass that may be used in biofuel and bioproduct production. The methods allows the coexistence of the pretreated lignocellulosic biomass and the enzymes during storage and transporation, the immediate hydrolysis of the pretreated lignocellulosic biomass to produce sugars, without further addition of enzymes, in a biofuel or bioproduct production site, the enhancement of the final hydrolytic activity of the pretreated lignocellulosic biomass, and/or the reduction in sensitivity of the inhibitors in the pretreated lignocellulosic biomass.
摘要翻译：本发明提供了生产预处理的木质纤维素生物质与酶结合的方法，用于储存和运输可用于生物燃料和生物产物生产的预处理的木质纤维素生物质。这些方法允许在储存和运输过程中预处理的木质纤维素生物质和酶的共存，预处理的木质纤维素生物质在生物燃料或生物产物生产位点中立即水解而不进一步加入酶以产生糖，增强最终的水解预处理的木质纤维素生物质的活性和/或抑制剂在预处理的木质纤维素生物质中的敏感性的降低。

19. 发明授权

US06465635B2 Promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species 失效
标题翻译：产生乳酸的真菌根霉的3-磷酸甘油酸激酶基因1的启动子序列和在真菌种中表达目的基因的方法
公开(公告)号：US06465635B2
公开(公告)日：2002-10-15
申请号：US09536034
申请日：2000-03-27
申请人： Johnway Gao , Rodney S. Skeen
发明人： Johnway Gao , Rodney S. Skeen
IPC分类号： C07H2104
CPC分类号： C12N9/1217 , C07K14/37 , C12N15/80
摘要： The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 1 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.
摘要翻译：本发明提供了产生乳酸的丝状真菌菌株，米根霉（Rhizopus oryzae）的磷酸甘油酸激酶基因1的启动子克隆发现。分离的启动子可以在各种碳水化合物条件下组成型调节基因表达。此外，本发明还提供了用于在米根霉（Rhizopus oryzae）中外源基因转化的整合载体的设计。

20. 发明授权

US08389243B2 Methods of spraying saccharification enzymes and fermentation organisms onto lignocellulosic biomass for hydrolysis and fermentation processes 失效
标题翻译：将糖化酶和发酵生物体喷洒到木质纤维素生物质用于水解和发酵过程的方法
公开(公告)号：US08389243B2
公开(公告)日：2013-03-05
申请号：US12816999
申请日：2010-06-16
申请人： Johnway Gao , Benjamin Levie , Dwight Anderson
发明人： Johnway Gao , Benjamin Levie , Dwight Anderson
IPC分类号： C12P19/00 , C12P1/02
CPC分类号： C12P19/02 , C12P7/10 , C12P19/14 , C13K1/02 , Y02E50/16
摘要： The present invention provides spray methods of delivering saccharification enzymes, fermentation organisms, and other hydrolysis or fermentation ingredients onto lignocellulosic biomass. The methods reduce the need for mechanical mixing when the biomass solids are undergoing enzymatic hydrolysis, and reduce dilution to allow higher product titers in the hydrolysis and/or fermentation steps.
摘要翻译：本发明提供将糖化酶，发酵生物和其它水解或发酵成分递送到木质纤维素生物质上的喷雾方法。当生物质固体进行酶水解时，该方法减少了对机械混合的需要，并且减少稀释以允许水解和/或发酵步骤中更高的产物滴度。

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