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11. 发明申请

US20130115694A1 FORMATION OF NEUROMUSCULAR JUNCTIONS IN A DEFINED SYSTEM 有权
标题翻译：在定义的系统中形成神经结节
公开(公告)号：US20130115694A1
公开(公告)日：2013-05-09
申请号：US13696396
申请日：2011-05-06
申请人： James Hickman , Xiufang Guo
发明人： James Hickman , Xiufang Guo
IPC分类号： C12N5/071
CPC分类号： G01N33/5058 , C12N5/0697 , C12N2500/90 , C12N2501/01 , C12N2501/105 , C12N2501/13 , C12N2501/385 , C12N2501/41 , C12N2501/58 , C12N2502/081 , C12N2502/1335 , C12N2533/20 , C12N2535/10 , G01N33/48728 , G01N33/6872 , G01N33/6887 , G01N2500/10
摘要： A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more human muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a human muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.
摘要翻译：形成神经肌肉接头的方法包括通过在基本上无血清培养基中共培养一种或多种人类运动神经元和一种或多种人肌肉细胞，在运动神经元和肌肉细胞之间形成功能性神经肌肉接头。合成的哺乳动物神经肌肉接头包括在基本上无血清培养基中与人肌肉细胞功能性连接的人运动神经元。可以使用人造基底来支撑一个或多个神经肌肉接头。

12. 发明授权

US09404140B1 Patterned cardiomyocyte culture on microelectrode array 有权
标题翻译：图案化心肌细胞培养在微电极阵列上
公开(公告)号：US09404140B1
公开(公告)日：2016-08-02
申请号：US12938701
申请日：2010-11-03
申请人： Peter Molnar , Anupama Natarajan , Maria Stancescu , James Hickman
发明人： Peter Molnar , Anupama Natarajan , Maria Stancescu , James Hickman
IPC分类号： C12Q1/02 , C12Q1/04 , G01N33/50
CPC分类号： G01N33/4836 , C12N5/0657 , C12N2533/30 , C12N2535/10 , C12Q1/02 , C12Q1/04 , G01N33/48728 , G01N33/5008 , G01N33/5061
摘要： The invention provides a culture of patterned cardiomyocytes, the culture including a support substrate bearing a multielectrode array, a negative surface resistant to cell attachment and deposited on the support substrate covering the multi-electrode array, a pattern ablated on the negative surface, a positive surface promoting cell attachment deposited on the pattern ablated on the negative surface; and cardiomyocytes adherent to the positive surface and growing aligned along the pattern ablated on the negative surface. The invention also includes a method of making the culture of patterned cardiomyocytes and a method of in vitro method of testing the effect of a compound on cardiac function by measuring electrical output from the patterned cardiomyocytes.
摘要翻译：本发明提供了一种图案化心肌细胞的培养物，其培养物包括支持多电极阵列的支撑基底，抵抗细胞附着并沉积在覆盖多电极阵列的支撑基底上的负表面，在负表面上烧蚀的图案，阳性沉积在图案上的表面促进细胞附着物消融在负面上; 并且附着在阳性表面上的生长对准的心肌细胞沿消融在阴性表面上的图案生长。本发明还包括使图案化心肌细胞培养的方法和通过测量来自图案化心肌细胞的电输出来测试化合物对心脏功能的影响的体外方法。

13. 发明申请

US20130096888A1 Model and Methods for Identifying Points of Action in Electrically Active Cells 有权
标题翻译：识别电活动细胞中的作用点的模型和方法
公开(公告)号：US20130096888A1
公开(公告)日：2013-04-18
申请号：US13576442
申请日：2011-02-07
申请人： James Hickman , Peter Molnar , Frank Sommerhage , Jonathan Hood , Jerry Jenkins
发明人： James Hickman , Peter Molnar , Frank Sommerhage , Jonathan Hood , Jerry Jenkins
IPC分类号： G06F17/50
CPC分类号： G06F17/5009 , G01N33/5058 , G06F19/12
摘要： The invention provides a model for generating predicted action potentials of an electrically active cell. The disclosed model includes three operatively coupled submodels. A first submodel contains Hodgkin-Huxley elements generating action potentials based on electrical equivalent circuits. A second submodel is based on reaction kinetics of cell metabolism and is operatively coupled with the first submodel. A third submodel is based on Boolean dynamics representing signaling and associated cellular processes and is operatively coupled with the first and second submodels. The invention includes storing a library of calculated action potentials and associated cellular parameters generated by the model, applying a stimulus to the electrically active cell in vitro so that the cell generates an action potential; and comparing the cell-generated action potential with those stored in the library, wherein a match is predictive of the cellular point of action of the applied stimulus according to the parameters stored.
摘要翻译：本发明提供了一种用于产生电活性电池的预测动作电位的模型。所公开的模型包括三个可操作耦合的子模型。第一个子模型包含Hodgkin-Huxley元素，其基于电等效电路产生动作电位。第二子模型基于细胞代谢的反应动力学，并且与第一子模型操作性耦合。第三子模型基于表示信令和相关联的细胞过程的布尔动力学，并且与第一和第二子模型可操作地耦合。本发明包括存储由模型产生的计算的动作电位和相关联的细胞参数的文库，在体外对电活性细胞施加刺激，使得细胞产生动作电位; 以及将细胞产生的动作电位与库中存储的动作电位进行比较，其中匹配是根据所存储的参数来预测所施加的刺激的细胞作用点。

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