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11. 发明申请

US20050227314A1 Polypeptide cleavage process 失效
标题翻译：多肽切割过程
公开(公告)号：US20050227314A1
公开(公告)日：2005-10-13
申请号：US10997822
申请日：2004-11-24
申请人： Barton Holmquist , Daniel Strydom , Jin Seo , Kirk Cryer
发明人： Barton Holmquist , Daniel Strydom , Jin Seo , Kirk Cryer
IPC分类号： C07F15/00 , C07K1/12 , C07K7/06 , C07K14/60 , C07K14/635 , C12P21/06
CPC分类号： C07K14/635 , C07K1/12 , C07K14/60
摘要： The invention provides a palladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladium promoter dissolved in a high-concentration formic acid, wherein the concentration of the formic acid in the reaction mixture is between about 1 to about 22 molar.
摘要翻译：本发明提供了钯促进的水解多肽切割方法，其在Cys-His切割位点选择性切割多肽，包括将溶解于多肽中的多肽溶解在由溶解在高浓度甲酸中的钯促进剂组成的反应混合物中，反应混合物中的甲酸为约1至约22摩尔。

12. 发明申请

US20050287632A1 Peptide amidation process 有权
标题翻译：肽酰胺化过程
公开(公告)号：US20050287632A1
公开(公告)日：2005-12-29
申请号：US10997081
申请日：2004-11-24
申请人： Barton Holmquist , Daniel Strydom
发明人： Barton Holmquist , Daniel Strydom
IPC分类号： C12N15/09 , C07K1/00 , C07K1/107 , C07K5/103 , C07K5/107 , C07K5/113 , C07K7/06 , C07K7/08 , C07K14/575 , C07K14/60 , C12N1/21 , C12P21/06
CPC分类号： C07K1/126 , C07K1/003 , C07K5/1008 , C07K5/1016 , C07K5/1021 , C07K7/06
摘要： The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxylterminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.
摘要翻译：本发明提供了一种酰胺化所需肽的方法，其包括在X 1 - 半胱氨酸序列上切割底物多肽，其中X1是肽羧基末端的氨基酸，半胱氨酸是第一个氨基酸包含序列半胱氨酸-X 2 -X 3的钯裂解位点，其中X 2是任何氨基酸，X 3是选自半胱氨酸，组氨酸或甲硫氨酸的氨基酸，并且其中所述肽的羧基末端在裂解时被酰胺化 X1半胱氨酸序列。

13. 发明申请

US20050260701A1 Method for enzymatic production of GLP-1 (7-36) amide peptides 审中-公开
标题翻译： GLP-1（7-36）酰胺肽酶促生产方法
公开(公告)号：US20050260701A1
公开(公告)日：2005-11-24
申请号：US10997061
申请日：2004-11-24
申请人： Fred Wagner , Peng Luan , Yuannan Xia , Mary Bossard , Barton Holmquist , Edwin Merrifield , Daniel Strydom
发明人： Fred Wagner , Peng Luan , Yuannan Xia , Mary Bossard , Barton Holmquist , Edwin Merrifield , Daniel Strydom
IPC分类号： C07K14/605 , C12P21/06
CPC分类号： C12P21/06 , C07K14/605
摘要： The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.
摘要翻译：本发明提供了使用clostripain从含有至少一个拷贝的肽的多肽制备肽从多肽中切下肽的方法。这些方法使得能够使用单一的高效酶裂解产生任何所需的肽序列。

14. 发明申请

US20090215987A1 PEPTIDE AMIDATION PROCESS 有权
标题翻译：肽辅助方法
公开(公告)号：US20090215987A1
公开(公告)日：2009-08-27
申请号：US12199902
申请日：2008-08-28
申请人： Barton Holmquist , Daniel Strydom
发明人： Barton Holmquist , Daniel Strydom
IPC分类号： C07K1/107
CPC分类号： C07K1/126 , C07K1/003 , C07K5/1008 , C07K5/1016 , C07K5/1021 , C07K7/06
摘要： The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxyl-terminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.
摘要翻译：本发明提供了一种酰化所需肽的方法，其包括在X1-半胱氨酸序列处切割底物多肽，其中X1是肽羧基末端的氨基酸，半胱氨酸是包含序列半胱氨酸的钯切割位点的第一个氨基酸 -X2-X3，其中X2是任何氨基酸，X3是选自半胱氨酸，组氨酸或甲硫氨酸的氨基酸，并且其中在X1-半胱氨酸序列切割时肽的羧基末端酰胺化。

15. 发明授权

US07462690B2 Peptide amidation process 有权
标题翻译：肽酰胺化过程
公开(公告)号：US07462690B2
公开(公告)日：2008-12-09
申请号：US10997081
申请日：2004-11-24
申请人： Barton Holmquist , Daniel Strydom
发明人： Barton Holmquist , Daniel Strydom
IPC分类号： C07K1/107
CPC分类号： C07K1/126 , C07K1/003 , C07K5/1008 , C07K5/1016 , C07K5/1021 , C07K7/06
摘要： The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino acid, X3 is an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxylterminus of the peptide is amidated upon cleavage at the X1-cysteine sequence.
摘要翻译：本发明提供了一种酰化所需肽的方法，其包括在X1-半胱氨酸序列处切割底物多肽，其中X1是肽羧基末端的氨基酸，半胱氨酸是包含序列半胱氨酸的钯切割位点的第一个氨基酸 -X2-X3，其中X2是任何氨基酸，X3是选自半胱氨酸，组氨酸或甲硫氨酸的氨基酸，并且其中在X1-半胱氨酸序列切割时，肽的羧基末端被酰胺化。

16. 发明申请

US20060008870A1 Method for universal enzymatic production of bioactive peptides 失效
公开(公告)号：US20060008870A1
公开(公告)日：2006-01-12
申请号：US10997074
申请日：2004-11-24
申请人： Fred Wagner , Peng Luan , Yuannan Xia , Daniel Strydom , Edwin Merrifield , Mary Bossard , Barton Holmquist , Jin Seo
发明人： Fred Wagner , Peng Luan , Yuannan Xia , Daniel Strydom , Edwin Merrifield , Mary Bossard , Barton Holmquist , Jin Seo
IPC分类号： C12P21/06 , C12N9/64
CPC分类号： C07K14/605 , C12N9/52 , C12P21/06
摘要： The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.

17. 发明申请

US20050244924A1 Methods and constructs for high yield expression of clostripain 失效
标题翻译： clostripain的高产量表达的方法和构建体
公开(公告)号：US20050244924A1
公开(公告)日：2005-11-03
申请号：US10997697
申请日：2004-11-24
申请人： Fred Wagner , Peng Luan , Yuannan Xia , Barton Holmquist
发明人： Fred Wagner , Peng Luan , Yuannan Xia , Barton Holmquist
IPC分类号： C07H21/04 , C07K14/47 , C12N1/21 , C12N9/50 , C12N15/67 , C12N15/74 , C12P21/06
CPC分类号： C12N9/50 , C12N15/67
摘要： The invention provides methods and nucleic acid constructs to express clostripain.
摘要翻译：本发明提供了表达clostripain的方法和核酸构建体。

18. 发明授权

US06461834B1 Clostripain catalyzed amidation of peptides 失效
标题翻译： Clostripain催化肽的酰胺化
公开(公告)号：US06461834B1
公开(公告)日：2002-10-08
申请号：US09212663
申请日：1998-12-16
申请人： Dan Dormady , Jay S. Stout , Daniel J. Strydom , Barton Holmquist , Fred W. Wagner
发明人： Dan Dormady , Jay S. Stout , Daniel J. Strydom , Barton Holmquist , Fred W. Wagner
IPC分类号： C12P2106
CPC分类号： C07K14/605 , C12P21/06
摘要： The invention provides a method of producing a polypeptide having a C-terminal &agr;-carboxamide group. It particularly concerns an enzymatic modification of selected substrate polypeptides which result in cleavage of the substrate polypeptide to form a product peptide with a C-terminal arginine residue having an &agr;-carboxamide group (C-terminal “Arg-NH2”). The method includes contacting an aqueous-based solution including (i) ammonia reagent and (ii) the substrate polypeptide with (iii) clostripain.
摘要翻译：本发明提供了产生具有C-末端α-甲酰胺基团的多肽的方法。它特别涉及所选择的底物多肽的酶修饰，其导致底物多肽切割以形成具有α-甲酰胺基团（C-末端“Arg-NH 2”）的C-末端精氨酸残基的产物肽。该方法包括使含有（i）氨试剂和（ii）底物多肽的水性溶液与（iii）clostripain接触。

19. 发明授权

US4908187A Device for diluting and mixing liquids and applications for kinetic analysis 失效
标题翻译：用于稀释和混合液体的装置和动力学分析的应用
公开(公告)号：US4908187A
公开(公告)日：1990-03-13
申请号：US228786
申请日：1988-08-04
申请人： Barton Holmquist , Robert K. Scopes
发明人： Barton Holmquist , Robert K. Scopes
IPC分类号： B01F3/08 , B01F13/00 , G01N1/38
CPC分类号： B01F13/0016 , B01F3/08 , G01N1/38
摘要： This invention relates to a diluting and mixing device which is capable of diluting a first solution to produce a second solution which is mixed with an undiluted third solution, to reproducibly produce a unique series of combined solutions. Each solution in said series of combined solutions may vary only in the concentration of a single (selected) reactant, and typically, each successive solution becomes increasingly more concentrated in the selected reactant. By employing a modification in procedure, each successive solution in said series may become decreasingly less concentrated in the selected reactant.This invention further relates to an automated system comprising the device connected to a stepping motor so as to rapidly and reproducibly produce said series of solutions, said device being further connected to an analyzer means for obtaining chemical, biochemical, or physical chemical data on said series of solutions. This invention especially relates to an embodiment of the device for obtaining kinetic data on enzyme reactions in solutions.The device is useful because it provides a means for rapidly obtaining chemical, biochemical, or physical chemical data on reactions in solution.
摘要翻译：本发明涉及稀释和混合装置，其能够稀释第一溶液以产生与未稀释的第三溶液混合的第二溶液，以可重复地产生独特的一系列组合溶液。所述一系列组合溶液中的每种溶液可以仅在单一（选择的）反应物的浓度上变化，并且通常，每个连续的溶液变得越来越浓缩在所选择的反应物中。通过采用程序的改进，所述系列中的每个连续溶液可能在所选择的反应物中的浓度越来越少。本发明还涉及一种自动化系统，其包括连接到步进电机的装置，以便快速且可重复地产生所述一系列解决方案，所述装置进一步连接到用于获得所述系列上的化学，生物化学或物理化学数据的分析装置的解决方案。本发明特别涉及用于获得溶液中酶反应的动力学数据的装置的实施方案。该设备是有用的，因为它提供了快速获得溶液中反应的化学，生物化学或物理化学数据的手段。

20. 发明授权

US08003362B2 Methods and constructs for high yield expression of clostripain 失效
标题翻译： clostripain的高产量表达的方法和构建体
公开(公告)号：US08003362B2
公开(公告)日：2011-08-23
申请号：US10997697
申请日：2004-11-24
申请人： Fred W. Wagner , Peng Luan , Yuannan Xia , Barton Holmquist
发明人： Fred W. Wagner , Peng Luan , Yuannan Xia , Barton Holmquist
IPC分类号： C12N9/48 , C12N1/20 , C12N15/74 , C07K1/00
CPC分类号： C12N9/50 , C12N15/67
摘要： The invention provides methods and nucleic acid constructs to express clostripain. The source of the coding region for recombinantly expressed clostripain is Clostridium histolyticum.
摘要翻译：本发明提供了表达clostripain的方法和核酸构建体。用于重组表达的clostripain的编码区的来源是溶组织梭菌（Clostridium histolyticum）。

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