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11. 发明申请

US20110229938A1 Method For Amplifying Locus In Bacterial Cell 有权
标题翻译：在细菌细胞中放大轨迹的方法
公开(公告)号：US20110229938A1
公开(公告)日：2011-09-22
申请号：US12934630
申请日：2009-03-27
申请人： Eugenio Ferrari , Caroline M. Peres
发明人： Eugenio Ferrari , Caroline M. Peres
IPC分类号： C12P19/34 , C12N1/21
CPC分类号： C12N15/75
摘要： Certain aspects of this disclosure relate to a method of amplifying a genomic locus. In certain embodiments, the method may comprise: a) contacting a population of bacterial host cells with an inhibitor of an essential enzyme, where the bacterial host cells comprise a genomic locus of the structure: A1-P-M-A2, where A1 and A2 are direct repeats, P comprises a coding sequence for a polypeptide, and M comprises a coding sequence for the essential enzyme; and b) selecting for cells that are resistant to the inhibitor; wherein cells that are resistant to the inhibitor have multiple copies of the amplification unit.
摘要翻译：本公开的某些方面涉及扩增基因组座位的方法。在某些实施方案中，该方法可以包括：a）使细菌宿主细胞群与必需酶的抑制剂接触，其中细菌宿主细胞包含以下结构的基因组座位：A1-PM-A2，其中A1和A2为直接重复，P包含多肽的编码序列，M包含必需酶的编码序列; 和b）选择抗抑制剂的细胞; 其中对抑制剂具有抗性的细胞具有多个拷贝的扩增单元。

12. 发明授权

US09528114B2 Method for amplifying locus in bacterial cell 有权
标题翻译：扩增细菌细胞基因座的方法
公开(公告)号：US09528114B2
公开(公告)日：2016-12-27
申请号：US12934630
申请日：2009-03-27
申请人： Eugenio Ferrari , Caroline M. Peres
发明人： Eugenio Ferrari , Caroline M. Peres
IPC分类号： C12N15/75
CPC分类号： C12N15/75
摘要： Certain aspects of this disclosure relate to a method of amplifying a genomic locus. In certain embodiments, the method may comprise: a) contacting a population of bacterial host cells with an inhibitor of an essential enzyme, where the bacterial host cells comprise a genomic locus of the structure: A1-P-M-A2, where A1 and A2 are direct repeats, P comprises a coding sequence for a polypeptide, and M comprises a coding sequence for the essential enzyme; and b) selecting for cells that are resistant to the inhibitor; wherein cells that are resistant to the inhibitor have multiple copies of the amplification unit.
摘要翻译：本公开的某些方面涉及扩增基因组座位的方法。在某些实施方案中，该方法可以包括：a）使细菌宿主细胞群与必需酶的抑制剂接触，其中细菌宿主细胞包含以下结构的基因组座位：A1-PM-A2，其中A1和A2是直接重复，P包含多肽的编码序列，M包含必需酶的编码序列; 和b）选择抗抑制剂的细胞; 其中对抑制剂具有抗性的细胞具有多个拷贝的扩增单元。

13. 发明授权

US08895288B2 Methods of producing isoprene and a co-product 有权
标题翻译：生产异戊二烯和共同产品的方法
公开(公告)号：US08895288B2
公开(公告)日：2014-11-25
申请号：US12650332
申请日：2009-12-30
申请人： Gopal K. Chotani , Caroline M. Peres , Derek H. Wells , Karl J. Sanford , Joseph C. McAuliffe
发明人： Gopal K. Chotani , Caroline M. Peres , Derek H. Wells , Karl J. Sanford , Joseph C. McAuliffe
IPC分类号： C12N1/21 , C12P5/00 , C12N9/88 , C12P5/02 , C12P7/18
CPC分类号： C12P5/007 , C12N9/88 , C12P7/06 , C12P7/065 , C12P7/18 , Y02E50/17
摘要： The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.
摘要翻译：本发明的特征在于从培养细胞产生异戊二烯和共产物如乙醇，1,3-丙二醇或氢的方法。本发明还提供包括这些培养细胞的组合物。本发明提供了包含异戊二烯和乙醇，异戊二烯和1,3-丙二醇以及异戊二烯和氢的组合物。另外，本发明提供了通过在适合共同生产异戊二烯和乙醇，异戊二烯和1,3-丙二醇的条件下培养细胞来共同生产异戊二烯和乙醇，异戊二烯和1,3-丙二醇以及异戊二烯和氢的方法，以及异戊二烯和氢。

14. 发明授权

US08569026B2 Systems using cell culture for production of isoprene 有权
公开(公告)号：US08569026B2
公开(公告)日：2013-10-29
申请号：US12560366
申请日：2009-09-15
申请人： Gopal K. Chotani , Joseph C. McAuliffe , Caroline M. Peres , Gregory M. Whited
发明人： Gopal K. Chotani , Joseph C. McAuliffe , Caroline M. Peres , Gregory M. Whited
IPC分类号： C12P5/02 , C12M1/04 , C12N9/16
CPC分类号： C12P5/007 , C12P5/026
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity. Additionally, the invention provides methods of producing isoprene by culturing cells under conditions suitable for isoprene production while maintaining cell viability and/or metabolic activity.

15. 发明授权

US08288148B2 Compositions and methods for producing isoprene 有权
标题翻译：用于生产异戊二烯的组合物和方法
公开(公告)号：US08288148B2
公开(公告)日：2012-10-16
申请号：US12335071
申请日：2008-12-15
申请人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
发明人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
IPC分类号： C12N1/20 , C12N15/74 , C12N15/70 , C12P7/42 , C12P5/02 , C07H21/04
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
摘要翻译：本发明的特征在于从培养细胞产生异戊二烯的方法。本发明还提供包括这些培养细胞的组合物。

16. 发明申请

US20100196977A1 METHODS OF PRODUCING ISOPRENE AND A CO-PRODUCT 有权
标题翻译：生产异丙酚和副产品的方法
公开(公告)号：US20100196977A1
公开(公告)日：2010-08-05
申请号：US12650332
申请日：2009-12-30
申请人： Gopal K. Chotani , Caroline M. Peres , Derek H. Wells , Karl J. Sanford , Joseph C. McAuliffe
发明人： Gopal K. Chotani , Caroline M. Peres , Derek H. Wells , Karl J. Sanford , Joseph C. McAuliffe
IPC分类号： C12P7/18 , C12P7/06 , C12P5/02 , C12N1/21 , C12N1/19
CPC分类号： C12P5/007 , C12N9/88 , C12P7/06 , C12P7/065 , C12P7/18 , Y02E50/17
摘要： The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.
摘要翻译：本发明的特征在于从培养细胞产生异戊二烯和共产物如乙醇，1,3-丙二醇或氢的方法。本发明还提供包括这些培养细胞的组合物。本发明提供了包含异戊二烯和乙醇，异戊二烯和1,3-丙二醇以及异戊二烯和氢的组合物。另外，本发明提供了通过在适合共同生产异戊二烯和乙醇，异戊二烯和1,3-丙二醇的条件下培养细胞来共同生产异戊二烯和乙醇，异戊二烯和1,3-丙二醇以及异戊二烯和氢的方法，以及异戊二烯和氢。

17. 发明申请

US20130260432A1 ISOPRENE SYNTHASE VARIANTS FOR IMPROVED MICROBIAL PRODUCTION OF ISOPRENE 有权
公开(公告)号：US20130260432A1
公开(公告)日：2013-10-03
申请号：US13436612
申请日：2012-03-30
申请人： Richard R. BOTT , Marguerite A. Cervin , James T. Kellis, JR. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. BOTT , Marguerite A. Cervin , James T. Kellis, JR. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
IPC分类号： C12N9/88 , C12P5/00
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.

18. 发明申请

US20130071908A1 COMPOSITIONS AND METHODS FOR PRODUCING ISOPRENE 有权
公开(公告)号：US20130071908A1
公开(公告)日：2013-03-21
申请号：US13600109
申请日：2012-08-30
申请人： Marguerite A. CERVIN , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Karl J. Sanford , Joseph C. McAuliffe , Frank J. Feher , Aaron S. Puhala , Andrei Miasnikov , Richard La Duca , Caroline M. Peres
发明人： Marguerite A. CERVIN , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Karl J. Sanford , Joseph C. McAuliffe , Frank J. Feher , Aaron S. Puhala , Andrei Miasnikov , Richard La Duca , Caroline M. Peres
IPC分类号： C12N1/15 , C12N1/19
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

19. 发明授权

US09260727B2 Compositions and methods for producing isoprene 有权
标题翻译：用于生产异戊二烯的组合物和方法
公开(公告)号：US09260727B2
公开(公告)日：2016-02-16
申请号：US13600132
申请日：2012-08-30
申请人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
发明人： Marguerite A. Cervin , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
IPC分类号： C12N1/20 , C12P7/42 , C12P5/02 , C12N15/00 , C12N15/74 , C07H21/04 , C12P5/00 , C12N9/10 , C12N9/12 , C12N9/88 , C12N9/90
CPC分类号： C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
摘要翻译：本发明的特征在于从培养细胞产生异戊二烯的方法。本发明还提供包括这些培养细胞的组合物。

20. 发明授权

US08173410B2 Isoprene synthase variants for improved microbial production of isoprene 有权
标题翻译：异戊二烯合成酶变体用于改善异戊二烯的微生物产生
公开(公告)号：US08173410B2
公开(公告)日：2012-05-08
申请号：US12429143
申请日：2009-04-23
申请人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
发明人： Richard R. Bott , Marguerite A. Cervin , James T. Kellis, Jr. , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Christopher Lee Rife , Derek H. Wells , Walter Weyler , Gregory M. Whited
IPC分类号： C12N9/88 , C12N15/09 , C12N15/00 , C12N1/20 , C12P5/02 , C07H21/04 , C07K1/00
CPC分类号： C12N9/88 , C12P5/007 , C12Y402/03027
摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
摘要翻译：本发明提供包含至少一种具有改善的催化活性和/或溶解度的异戊二烯合酶的方法和组合物。特别地，本发明提供用于在微生物宿主细胞中增加异戊二烯生产的变体植物异戊二烯合酶。本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。

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