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    • 19. 发明授权
    • Method of inducing differentiation of embryonic stem cells into hemangioblast
    • 诱导胚胎干细胞分化为成血管细胞的方法
    • US08507275B2
    • 2013-08-13
    • US12858747
    • 2010-08-18
    • Yong-Mahn HanSang-Wook ParkEun-Young Lee
    • Yong-Mahn HanSang-Wook ParkEun-Young Lee
    • C12N5/00C12N5/02C12N5/08A01N61/00A01N37/18
    • C12N5/069C12N5/0647C12N5/0691C12N5/0692C12N2501/115C12N2501/155C12N2501/165C12N2501/999C12N2506/02C12N2506/28
    • The present invention relates to a composition for inducing embryonic stem cell differentiation comprising a MEK/ERK (mitogen-activated protein kinase kinase/extracellular regulated kinase) signal transduction inhibitor and BMP (bone morphogenetic protein), and a method for inducing differentiation of embryonic stem cells into mesodermal cells using the same. Further, the mesodermal cells obtained by the above method are able to differentiate into various mesenchymal tissue cells. In particular, the present invention relates to a method for inducing differentiation into hemangioblast by culturing the mesodermal cells obtained by the above method in the presence of VEGF (vascular endothelial cell growth factor) and bFGF (basic fibroblast growth factor). The differentiated hemangioblasts can be further differentiated into vascular endothelial cells, vascular smooth muscle cells, and hematopoietic stem cells under various culture conditions.
    • 本发明涉及一种用于诱导胚胎干细胞分化的组合物,其包含MEK / ERK(丝裂原活化蛋白激酶激酶/细胞外调节激酶)信号转导抑制剂和BMP(骨形态发生蛋白)),以及诱导胚胎干细胞分化的方法 细胞使用相同的细胞进入中胚层细胞。 此外,通过上述方法获得的中胚层细胞能够分化成各种间充质组织细胞。 特别地,本发明涉及通过在VEGF(血管内皮细胞生长因子)和bFGF(碱性成纤维细胞生长因子)的存在下培养通过上述方法获得的中胚层细胞来诱导分化为成血管细胞成分的方法。 分化的成血管细胞可以在各种培养条件下进一步分化成血管内皮细胞,血管平滑肌细胞和造血干细胞。