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11. 发明申请

US20090053251A1 Dendritic Cell Compositions and Methods 有权
标题翻译：树突状细胞组成和方法
公开(公告)号：US20090053251A1
公开(公告)日：2009-02-26
申请号：US11918076
申请日：2006-04-07
申请人： Rebecca Pogue-Caley , Tamara Monesmith , Irina Tcherepanova , Lois Dinterman
发明人： Rebecca Pogue-Caley , Tamara Monesmith , Irina Tcherepanova , Lois Dinterman
IPC分类号： A61K39/00 , C12N5/08 , A61P31/00
CPC分类号： C12N5/0639 , A61K2039/5154 , C12N2501/02 , C12N2501/22 , C12N2501/23 , C12N2501/2301 , C12N2501/2304 , C12N2501/2306 , C12N2501/24 , C12N2501/25 , C12N2502/11 , C12N2506/11 , C12N2506/115 , C12N2523/00
摘要： Methods are provided for the production of dendritic cells from monocytes that have been incubated at a temperature of 1° C.-34° C. for a period of approximately 6 to 96 hours from the time they are isolated from a subject. After the incubation period, the monocytes can then be induced to differentiate into dendritic cells. Mature dendritic cells made by the methods of the invention have increased levels of one or more of CD80, CD83, CD86, MHC class I molecules, or MHC class II molecules as compared to mature dendritic cells prepared from monocytes that have not been held at 1° C.-34° C. for at least 6 hours from the time they were isolated from a subject. Dendritic cells made by the methods of the invention are useful for the preparation of vaccines and for the stimulation of T cells.
摘要翻译：提供了用于从单核细胞生产树突状细胞的方法，其已经在从受试者分离出的时间内在1℃-34℃的温度下温育约6至96小时。潜伏期后，可诱导单核细胞分化为树突状细胞。通过本发明的方法制备的成熟树突状细胞与从未保持在1的单核细胞制备的成熟树突状细胞相比，具有增加CD80，CD83，CD86，MHC I类分子或MHC II类分子中的一种或多种的水平从受试者分离出的时间至少6小时。通过本发明方法制备的树突状细胞可用于制备疫苗和刺激T细胞。

12. 发明申请

US20070248578A1 Mrna Transfected Antigen Presenting Cells 审中-公开
标题翻译：转铁蛋白抗原递呈细胞
公开(公告)号：US20070248578A1
公开(公告)日：2007-10-25
申请号：US10580597
申请日：2004-11-24
申请人： Irina Tcherepanova
发明人： Irina Tcherepanova
IPC分类号： C07H21/04 , A61K48/00
CPC分类号： A61K39/00 , A61K2039/5154 , A61K2039/5156 , A61K2039/53 , C12Q1/6865 , Y02A50/407 , Y02A50/41 , Y02A50/412 , Y02A50/466 , Y02A50/48 , Y02A50/56 , C12Q2525/173
摘要： A method of amplifying RNA to obtain RNA molecules that are predominantly in the sense orientation and essentially devoid of RNA molecules that are in the anti-sense orientation. A method of transfecting antigen presenting cells with a composition comprising sense RNA encoding immunogenic antigens and essentially devoid of antisense RNA and dsRNA is also provided as well as dendritic cells prepared according to the method.
摘要翻译：扩增RNA以获得主要在正义取向并且基本上没有处于反义方向的RNA分子的RNA分子的方法。还提供了用包含有义RNA编码免疫原性抗原并且基本上没有反义RNA和dsRNA的组合物转染抗原呈递细胞的方法以及根据该方法制备的树突状细胞。

13. 发明授权

US08501470B2 Dendritic cell compositions and methods 有权
标题翻译：树突状细胞的组成和方法
公开(公告)号：US08501470B2
公开(公告)日：2013-08-06
申请号：US13351510
申请日：2012-01-17
申请人： Rebecca Pogue-Caley , Tamara Monesmith , Irina Tcherepanova , Lois Dinterman
发明人： Rebecca Pogue-Caley , Tamara Monesmith , Irina Tcherepanova , Lois Dinterman
IPC分类号： C12N5/07
CPC分类号： C12N5/0639 , A61K2039/5154 , C12N2501/02 , C12N2501/22 , C12N2501/23 , C12N2501/2301 , C12N2501/2304 , C12N2501/2306 , C12N2501/24 , C12N2501/25 , C12N2502/11 , C12N2506/11 , C12N2506/115 , C12N2523/00
摘要： Methods are provided for the production of dendritic cells from monocytes that have been incubated at a temperature of 1° C.-34° C. for a period of approximately 6 to 96 hours from the time they are isolated from a subject. After the incubation period, the monocytes can then be induced to differentiate into dendritic cells. Mature dendritic cells made by the methods of the invention have increased levels of one or more of CD80, CD83, CD86, MHC class I molecules, or MHC class II molecules as compared to mature dendritic cells prepared from monocytes that have not been held at 1° C.-34° C. for at least 6 hours from the time they were isolated from a subject. Dendritic cells made by the methods of the invention are useful for the preparation of vaccines and for the stimulation of T cells.
摘要翻译：提供了用于从单核细胞生产树突状细胞的方法，其已经在从受试者分离的时间内在1℃-34℃的温度下孵育约6至96小时。潜伏期后，可诱导单核细胞分化为树突状细胞。通过本发明的方法制备的成熟树突状细胞与从未保持在1的单核细胞制备的成熟树突状细胞相比，具有增加CD80，CD83，CD86，MHC I类分子或MHC II类分子中的一种或多种的水平从受试者分离出的时间至少6小时。通过本发明方法制备的树突状细胞可用于制备疫苗和刺激T细胞。

14. 发明授权

US08221981B2 Primers and probes for the amplification and detection of HIV Gag, Rev and Nef polynucleotides 有权
标题翻译：用于扩增和检测HIV Gag，Rev和Nef多核苷酸的引物和探针
公开(公告)号：US08221981B2
公开(公告)日：2012-07-17
申请号：US12452580
申请日：2008-07-30
申请人： Irina Tcherepanova , Aijing Starr , Brad Lackford
发明人： Irina Tcherepanova , Aijing Starr , Brad Lackford
IPC分类号： C07H21/04 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/703 , C12Q2525/143 , C12Q2521/119
摘要： The invention relates to improved methods and compositions for the nucleic acid amplification of one or multiple variants (strains) of Human Immunodeficiency Virus (HIV) present in a sample, and preferably in a sample from a pathogen infected individual. In particular, novel primers, methods and kits for the amplification of one or more species of HIV Rev, Gag and Nef nucleic acids are provided. The amplified HIV nucleic acid can be used to identify and/or quantitate HIV variants present in a sample. Nucleic acids produced by the methods of the invention or the proteins encoded thereby can also be used directly as vaccines or to transfect/load antigen presenting cells. The loaded antigen presenting cells can be used as a vaccine for the treatment or prevention of HIV infection.
摘要翻译：本发明涉及用于核酸扩增样品中存在的人免疫缺陷病毒（HIV）的一个或多个变体（菌株），优选来自病原体感染个体的样品的改进方法和组合物。特别地，提供用于扩增一种或多种HIV Rev，Gag和Nef核酸的新引物，方法和试剂盒。扩增的HIV核酸可用于鉴定和/或定量样品中存在的HIV变体。通过本发明的方法产生的核酸或由其编码的蛋白质也可以直接用作疫苗或转染/加载抗原呈递细胞。负载的抗原呈递细胞可用作用于治疗或预防HIV感染的疫苗。

15. 发明申请

US20080311155A1 Strain-Independent Amplification of Pathogens and Vaccines Thereto 有权
标题翻译：病原菌和疫苗的应变无关扩增
公开(公告)号：US20080311155A1
公开(公告)日：2008-12-18
申请号：US11662828
申请日：2005-09-14
申请人： Charles Nicolette , Irina Tcherepanova , Jason Harris , Donald Healey
发明人： Charles Nicolette , Irina Tcherepanova , Jason Harris , Donald Healey
IPC分类号： A61K39/00 , C12N5/06 , C12N15/87 , C07H21/04 , C12P19/34 , A61P31/00
CPC分类号： C07K14/005 , A61K39/21 , A61K2039/5154 , A61K2039/5156 , C12N7/00 , C12N2740/16041 , C12N2740/16234 , C12N2740/16334 , C12Q1/703
摘要： This in invention relates to methods for the nucleic acid amplification of multiple variants (strains) of any pathogen present in a sample, and preferably in a sample from a pathogen infected individual. In preferred embodiments, the pathogen is a retrovirus, such as HIV. The amplified pathogen nucleic acid can be used to identify the pathogen variants present in a sample, to quantitate the pathogen present in a sample, and as a nucleic acid vaccine, or in the preparation of antigen presenting cell vaccines. Nucleic acids produced by the methods of the invention or the proteins encoded thereby can be used to transfect/load antigen presenting cells. The loaded antigen presenting cells can then be used as a vaccine for the treatment of pathogen infection. In another embodiment, nucleic acids produced by the methods of the invention can be used directly as nucleic acid vaccines without prior loading into antigen presenting cells.
摘要翻译：本发明涉及用于核酸扩增样品中存在的任何病原体的多种变体（菌株）的方法，优选来自病原体感染个体的样品。在优选的实施方案中，病原体是逆转录病毒，例如HIV。扩增的病原体核酸可用于鉴定样品中存在的病原体变体，以定量样品中存在的病原体，以及作为核酸疫苗或制备抗原呈递细胞疫苗。通过本发明的方法产生的核酸或由其编码的蛋白质可用于转染/负载抗原呈递细胞。然后将负载的抗原呈递细胞用作用于治疗病原体感染的疫苗。在另一个实施方案中，通过本发明的方法产生的核酸可以直接用作核酸疫苗而不预先加载到抗原呈递细胞中。

16. 发明申请

US20120231509A1 Primers and Probes for the Amplification and Detection of HIV GAG, REV and NEF Polynucleotides 有权
标题翻译：用于扩增和检测HIV GAG，REV和NEF多核苷酸的引物和探针
公开(公告)号：US20120231509A1
公开(公告)日：2012-09-13
申请号：US13479335
申请日：2012-05-24
申请人： Irina Tcherepanova , Aijing Starr , Brad Lackford
发明人： Irina Tcherepanova , Aijing Starr , Brad Lackford
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C12Q1/703 , C12Q2525/143 , C12Q2521/119
摘要： The invention relates to improved methods and compositions for the nucleic acid amplification of one or multiple variants (strains) of Human Immunodeficiency Virus (HIV) present in a sample, and preferably in a sample from a pathogen infected individual. In particular, novel primers, methods and kits for the amplification of one or more species of HIV Rev, Gag and Nef nucleic acids are provided. The amplified HIV nucleic acid can be used to identify and/or quantitate HIV variants present in a sample. Nucleic acids produced by the methods of the invention or the proteins encoded thereby can also be used directly as vaccines or to transfect/load antigen presenting cells. The loaded antigen presenting cells can be used as a vaccine for the treatment or prevention of HIV infection.
摘要翻译：本发明涉及用于核酸扩增样品中存在的人免疫缺陷病毒（HIV）的一个或多个变体（菌株），优选来自病原体感染个体的样品的改进方法和组合物。特别地，提供用于扩增一种或多种HIV Rev，Gag和Nef核酸的新引物，方法和试剂盒。扩增的HIV核酸可用于鉴定和/或定量样品中存在的HIV变体。通过本发明的方法产生的核酸或由其编码的蛋白质也可以直接用作疫苗或转染/加载抗原呈递细胞。负载的抗原呈递细胞可用作用于治疗或预防HIV感染的疫苗。

17. 发明申请

US20120114680A1 Dendritic Cell Compositions and Methods 有权
标题翻译：树突状细胞组成和方法
公开(公告)号：US20120114680A1
公开(公告)日：2012-05-10
申请号：US13351510
申请日：2012-01-17
申请人： Rebecca Pogue-Caley , Tamara Monesmith , Irina Tcherepanova , Lois Dinterman
发明人： Rebecca Pogue-Caley , Tamara Monesmith , Irina Tcherepanova , Lois Dinterman
IPC分类号： A61K39/00 , C12N5/0786 , A61P37/04 , A61P35/00 , A61K35/14 , C12N5/02 , C12N15/85
CPC分类号： C12N5/0639 , A61K2039/5154 , C12N2501/02 , C12N2501/22 , C12N2501/23 , C12N2501/2301 , C12N2501/2304 , C12N2501/2306 , C12N2501/24 , C12N2501/25 , C12N2502/11 , C12N2506/11 , C12N2506/115 , C12N2523/00
摘要： Methods are provided for the production of dendritic cells from monocytes that have been incubated at a temperature of 1° C.-34° C. for a period of approximately 6 to 96 hours from the time they are isolated from a subject. After the incubation period, the monocytes can then be induced to differentiate into dendritic cells. Mature dendritic cells made by the methods of the invention have increased levels of one or more of CD80, CD83, CD86, MHC class I molecules, or MHC class II molecules as compared to mature dendritic cells prepared from monocytes that have not been held at 1° C.-34° C. for at least 6 hours from the time they were isolated from a subject. Dendritic cells made by the methods of the invention are useful for the preparation of vaccines and for the stimulation of T cells.
摘要翻译：提供了用于从单核细胞生产树突状细胞的方法，其已经在从受试者分离的时间内在1℃-34℃的温度下孵育约6至96小时。潜伏期后，可诱导单核细胞分化为树突状细胞。通过本发明的方法制备的成熟树突状细胞与从未保持在1的单核细胞制备的成熟树突状细胞相比，具有增加CD80，CD83，CD86，MHC I类分子或MHC II类分子中的一种或多种的水平从受试者分离出的时间至少6小时。通过本发明方法制备的树突状细胞可用于制备疫苗和刺激T细胞。

18. 发明申请

US20100136631A1 IMPROVED PRIMERS AND PROBES FOR THE AMPLIFICATION AND DETECTION OF HIV GAG,REV AND NEF 有权
标题翻译：改善艾滋病病毒GAG，REV和NEF的扩增和检测的引导剂和检测
公开(公告)号：US20100136631A1
公开(公告)日：2010-06-03
申请号：US12452580
申请日：2008-07-30
申请人： Irina Tcherepanova
发明人： Irina Tcherepanova
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C12Q1/703 , C12Q2525/143 , C12Q2521/119
摘要： The invention relates to improved methods and compositions for the nucleic acid amplification of one or multiple variants (strains) of Human Immunodeficiency Virus (HIV) present in a sample, and preferably in a sample from a pathogen infected individual. In particular, novel primers, methods and kits for the amplification of one or more species of HIV Rev, Gag and Nef nucleic acids are provided. The amplified HIV nucleic acid can be used to identify and/or quantitate HIV variants present in a sample. Nucleic acids produced by the methods of the invention or the proteins encoded thereby can also be used directly as vaccines or to transfect/load antigen presenting cells. The loaded antigen presenting cells can be used as a vaccine for the treatment or prevention of HIV infection.
摘要翻译：本发明涉及用于核酸扩增样品中存在的人免疫缺陷病毒（HIV）的一个或多个变体（菌株），优选来自病原体感染个体的样品的改进方法和组合物。特别地，提供用于扩增一种或多种HIV Rev，Gag和Nef核酸的新引物，方法和试剂盒。扩增的HIV核酸可用于鉴定和/或定量样品中存在的HIV变体。通过本发明的方法产生的核酸或由其编码的蛋白质也可以直接用作疫苗或转染/加载抗原呈递细胞。负载的抗原呈递细胞可用作用于治疗或预防HIV感染的疫苗。

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