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11. 发明申请

US20050009187A1 Environmental stress-responsive promoter and genes encoding transcriptional factor 审中-公开
标题翻译：环境胁迫响应启动子和编码转录因子的基因
公开(公告)号：US20050009187A1
公开(公告)日：2005-01-13
申请号：US10495918
申请日：2002-11-15
申请人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
发明人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
IPC分类号： A01H5/00 , C07K14/415 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/82 , C07H21/04 , C12N15/63 , C12N15/87
CPC分类号： C07K14/415 , C12N15/8237 , C12N15/8238 , C12N15/8273
摘要： The present invention provides a stress responsive promoter. The environmental stress responsive promoter of the present invention comprises DNA of the following (a), (b) or (c):(a) DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90; (b) DNA consisting of a nucleotide sequence comprising a deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter; and (c) DNA hybridizing under stringent conditions to DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter.
摘要翻译：本发明提供一种应激反应启动子。本发明的环境胁迫响应启动子包含以下（a），（b）或（c）的DNA：（a）由选自SEQ ID NO：1至90的任何核苷酸序列组成的DNA; （b）由相对于选自SEQ ID NO：1至90的任何核苷酸序列缺失，取代或添加一个或多个核苷酸的核苷酸序列组成的DNA，并且起到环境胁迫响应启动子的作用; 和（c）在严格条件下与由选自SEQ ID NO：1至90的任何核苷酸序列组成的DNA杂交并用作环境胁迫响应启动子的DNA。

12. 发明申请

US20120272401A1 PLANT OVEREXPRESSING ABSCISIC ACID TRANSPORTER PROTEIN AND METHOD FOR PRODUCING THE SAME 审中-公开
标题翻译：植物过氧化物载体运输蛋白及其生产方法
公开(公告)号：US20120272401A1
公开(公告)日：2012-10-25
申请号：US13518303
申请日：2010-12-21
申请人： Takashi Kuromori , Kazuo Shinozaki
发明人： Takashi Kuromori , Kazuo Shinozaki
IPC分类号： A01H5/00 , A01H5/10 , A01H1/00 , C12N5/10
CPC分类号： C12N15/8271 , C07K14/415 , C12N15/8262
摘要： The present invention relates to a transgenic plant tolerant to environmental stress that comprises DNA encoding an exogenous abscisic acid (ABA) transporter protein in an expressible manner, a progeny thereof, or a cell, tissue or seed from such plant. The present invention also relates to a method for producing such a plant.
摘要翻译：本发明涉及耐受环境胁迫的转基因植物，其包含以可表达的方式编码外源脱落酸（ABA）转运蛋白的DNA，其后代或来自该植物的细胞，组织或种子。本发明还涉及这种植物的制造方法。

13. 发明申请

US20070174937A1 PLANT HAVING TOLERANCE TO ENVIRONMENTAL STRESS 审中-公开
标题翻译：具有环境压力的工厂
公开(公告)号：US20070174937A1
公开(公告)日：2007-07-26
申请号：US11677428
申请日：2007-02-21
申请人： Taishi Umezawa , Kazuo Shinozaki
发明人： Taishi Umezawa , Kazuo Shinozaki
IPC分类号： A01H1/00 , C07H21/04 , C07K14/415
CPC分类号： C12N15/8273 , C12N9/12 , C12N15/8271
摘要： This invention provides a stress tolerant transgenic plant with the utilization of a signal transduction factor located upstream of the gene group associated with environmental responses. This invention provides a gene encoding the following protein (a) or (b) and a stress tolerant transgenic plant to which such gene has been introduced: (a) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 2 in the Sequence Listing; or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 2 in the Sequence Listing by deletion. substitution, or addition of one or several amino acid residues and having activity of inducing expression of stress responsive transcription factors and activity of protein kinase.
摘要翻译：本发明提供了一种利用位于与环境反应相关的基因组上游的信号转导因子的胁迫耐受转基因植物。本发明提供了编码以下蛋白质（a）或（b）的基因和已经引入该基因的胁迫耐受性转基因植物：（a）由SEQ ID NO：2所示的氨基酸序列组成的蛋白质序列表; 或（b）由序列表中SEQ ID NO：2所示的氨基酸序列的氨基酸序列构成的蛋白质，通过缺失。取代或添加一个或几个氨基酸残基，并具有诱导应激反应转录因子表达和蛋白激酶活性的活性。

14. 发明申请

US20060200876A1 Method for increasing stress-resistance to a plant 有权
公开(公告)号：US20060200876A1
公开(公告)日：2006-09-07
申请号：US11405477
申请日：2006-04-18
申请人： Chieko Ohsumi , Teruaki Taji , Kazuo Shinozaki
发明人： Chieko Ohsumi , Teruaki Taji , Kazuo Shinozaki
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N9/1051 , C12N15/8271 , C12N15/8273
摘要： The present invention enables production of a plant resistant to environmental stresses including drought stress. The invention increases stress resistance to a plant by increasing raffinose content in the plant.

15. 发明申请

US20050221356A1 Process for producing ligand binding protein with the use of cell-free protein synthesis system and utilization thereof 审中-公开
标题翻译：使用无细胞蛋白质合成系统产生配体结合蛋白的方法及其利用
公开(公告)号：US20050221356A1
公开(公告)日：2005-10-06
申请号：US11081914
申请日：2005-03-17
申请人： Takanori Kigawa , Takayoshi Matsuda , Masaaki Aoki , Shigeyuki Yokoyama , Kazuo Shinozaki , Motoaki Seki
发明人： Takanori Kigawa , Takayoshi Matsuda , Masaaki Aoki , Shigeyuki Yokoyama , Kazuo Shinozaki , Motoaki Seki
IPC分类号： G01N33/50 , C12N15/09 , C12P19/34 , C12P21/00 , C12P21/02 , C12P21/06 , C12Q1/00 , C12Q1/68 , G01N33/15 , G01N33/53 , G01N33/566
CPC分类号： C12P21/02 , G01N2500/04
摘要： It is intended to provide a process for efficiently producing a ligand binding protein characterized in that this protein or its domain is synthesized in a cell-free protein synthesis system containing a ligand that binds to the protein or its domain. In a preferred embodiment, the above-described ligand is a substance selected from the group consisting of DNAs, RNAs, nucleosides, nucleotides, proteins, peptides, aminoacids, lipids, carbohydrates, derivatives thereof, alkaloids, terpenes, low-molecular weight compounds, coenzymes, low-molecular weight metal complexes and metal ions, while the ligand binding protein or the ligand binding domain thereof is synthesized as a soluble protein.
摘要翻译：旨在提供一种有效产生配体结合蛋白的方法，其特征在于该蛋白质或其结构域在含有与蛋白质或其结构域结合的配体的无细胞蛋白质合成系统中合成。在优选的实施方案中，上述配体是选自DNA，RNA，核苷，核苷酸，蛋白质，肽，氨基酸，脂质，碳水化合物，其衍生物，生物碱，萜烯，低分子量化合物，辅酶，低分子金属络合物和金属离子，而配体结合蛋白或其配体结合结构域作为可溶性蛋白质合成。

16. 发明申请

US20090282582A1 ENVIRONMENTAL STRESS RESPONSIVE PROMOTER AND METHOD OF TISSUE-SPECIFIC GENE EXPRESSION USING THE SAME 审中-公开
标题翻译：环境应激反应促进剂及使用其组织特异性基因表达的方法
公开(公告)号：US20090282582A1
公开(公告)日：2009-11-12
申请号：US12311900
申请日：2007-10-19
申请人： Motoaki Seki , Jong-Myong Kim , Kazuo Shinozaki , Miki Fujita
发明人： Motoaki Seki , Jong-Myong Kim , Kazuo Shinozaki , Miki Fujita
IPC分类号： C12N15/82 , C07H21/04 , C12N15/63 , A01H5/00 , C12N5/10
CPC分类号： C07K14/415 , C12N15/8237
摘要： This invention concerns induction of gene expression specifically in given tissue with the use of a novel promoter that has environmental stress responsiveness and functions specifically in given tissue. It is realized by a method comprising: a step of preparing a plant having an arbitrary gene downstream of the environmental stress responsive promoter comprising DNA (a), (b), or (c) below; (a) DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8; (b) DNA comprising a nucleotide sequence derived from the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 by deletion, substitution, or addition of one or a plurality of nucleotides and functioning as an environmental stress responsive promoter; or (c) DNA hybridizing under stringent conditions to DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 and functioning as an environmental stress responsive promoter; and a step of cultivating the plant in the presence of environmental stress, the method comprising inducing expression of the gene located downstream of the environmental stress responsive promoter in a tissue-specific manner.
摘要翻译：本发明涉及使用具有环境应激反应性和特定于给定组织中功能的新型启动子来特异性诱导给定组织中的基因表达。通过以下方法实现：包括下述DNA（a），（b）或（c）的环境胁迫响应启动子下游具有任意基因的植物的步骤; （a）包含SEQ ID NO：1至8中任一个所示的核苷酸序列的DNA; （b）通过缺失，取代或添加一个或多个核苷酸并起到环境应激反应启动子的作用，其包含衍生自SEQ ID NO：1至8中任一项所示核苷酸序列的核苷酸序列的DNA; 或（c）在严格条件下与包含SEQ ID NO：1至8中任一所示的核苷酸序列的DNA杂交并用作环境胁迫响应启动子的DNA的DNA; 以及在存在环境胁迫的情况下培育植物的步骤，所述方法包括以组织特异性方式诱导位于环境胁迫响应启动子下游的基因的表达。

17. 发明授权

US07608758B2 Plant system for comprehensive gene function analysis with the use of full-length cDNA 失效
标题翻译：使用全长cDNA进行综合基因功能分析的植物系统
公开(公告)号：US07608758B2
公开(公告)日：2009-10-27
申请号：US10488087
申请日：2002-08-29
申请人： Minami Matsui , Takanari Ichikawa , Miki Nakazawa , Motoaki Seki , Miki Fujita , Kazuo Shinozaki
发明人： Minami Matsui , Takanari Ichikawa , Miki Nakazawa , Motoaki Seki , Miki Fujita , Kazuo Shinozaki
IPC分类号： C12N15/82
CPC分类号： C12N15/8216
摘要： A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function includes (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to introduce the cDNA into the plants; (b) selecting a plant complying with desired selection requirements from the plants into which the cDNA has been introduced; (c) isolating the cDNA from the selected plant; and (d) re-introducing the thus isolated cDNA into a plant of the same species as the plant into which the cDNA has been introduced, to reconfirm a phenotype according to the selection requirements.
摘要翻译：一个易于指定表型的基因，从而全面分析基因功能。用于分析基因功能的方法包括（a）用含有表达调控序列的全长cDNA文库感染植物组，以将cDNA引入植物中; （b）从已经引入cDNA的植物中选择符合所需选择要求的植物; （c）从所选择的植物中分离cDNA; 和（d）将如此分离的cDNA重新引入与引入cDNA的植物相同物种的植物中，以根据选择要求重新确认表型。

18. 发明授权

US07368630B2 Environmental stress-responsive promoter and a gene encoding environmental stress-responsive transcriptional factor 失效
公开(公告)号：US07368630B2
公开(公告)日：2008-05-06
申请号：US11470154
申请日：2006-09-05
申请人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
发明人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
IPC分类号： C12N15/82 , A01H1/00
CPC分类号： C12N15/8238 , C07K14/415 , C12N15/8237 , C12N15/8273
摘要： The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.

19. 发明申请

US20130019340A1 PLANT RESISTANT TO ENVIRONMENTAL STRESS AND METHOD FOR PRODUCING THE SAME 审中-公开
标题翻译：耐环境应力的植物及其生产方法
公开(公告)号：US20130019340A1
公开(公告)日：2013-01-17
申请号：US13635390
申请日：2011-03-01
申请人： Kaoru Maruyama , Kazuo Shinozaki
发明人： Kaoru Maruyama , Kazuo Shinozaki
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8269 , C12N15/8271 , C12N15/8273
摘要： An object of the present invention is to provide a plant having environmental stress resistance. The present invention provides a transgenic plant having environmental stress resistance, in which an exogenous DNA encoding a transcription factor selected from the group consisting of transcription factors from Arabidopsis thaliana TCP13, TCP5 and TCP17, and homologous transcription factors thereof from other plants is overexpressed, and a method for producing such a plant.
摘要翻译：本发明的目的是提供一种具有耐环境胁迫性的植物。本发明提供了具有环境胁迫性的转基因植物，其中编码选自拟南芥TCP13，TCP5和TCP17的转录因子的转录因子的外源DNA以及来自其他植物的同源转录因子被过表达，一种生产这种植物的方法。

20. 发明申请

US20080216196A1 Environmental stress-responsive promoter and a gene encoding environmental stress-responsive transcriptional factor 审中-公开
标题翻译：环境应激反应启动子和编码环境应激反应转录因子的基因
公开(公告)号：US20080216196A1
公开(公告)日：2008-09-04
申请号：US12076932
申请日：2008-03-25
申请人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
发明人： Kazuo Shinozaki , Motoaki Seki , Miki Fujita
IPC分类号： C12N15/29 , C12N15/82
CPC分类号： C12N15/8238 , C07K14/415 , C12N15/8237 , C12N15/8273
摘要： The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.
摘要翻译：本发明提供一种通过（a）用具有SEQ ID NO：27的非生物环境胁迫应答启动子和其下游基因的表达载体制备重组植物细胞系，植物组织或植物来调节基因表达的方法 ; 和（b）在非生物环境胁迫下培养和培养重组植物细胞，植物组织或植物，其中所述启动子调节所述基因在非生物环境胁迫下的表达。

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