会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 13. 发明授权
    • Signal enhancement system with multiple labeled-moieties
    • 具有多个标记部分的信号增强系统
    • US07972837B2
    • 2011-07-05
    • US12042762
    • 2008-03-05
    • Helen LeeLing HuangMagda Anastassova DinevaHsiang Yun Hu
    • Helen LeeLing HuangMagda Anastassova DinevaHsiang Yun Hu
    • G01N33/53
    • G01N33/558Y10S435/81Y10S436/807Y10S977/70Y10S977/773Y10S977/788Y10S977/918
    • Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.
    • 描述了用于检测分析物的浸渍试验。 在优选的实施方案中,能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合,并且将被认为含有分析物的测试溶液弄脏。 分析物,生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。 在捕获区存在胶体金标记表示测试溶液中存在分析物。 使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级,并且胶体金标记的抗生物素抗体被恶化 量油尺在一个单独的步骤。 还描述了用于执行本发明的测试的套件。
    • 15. 发明申请
    • CAPTURE AND DETECTION FORMAT VERSATILITY FOR DIPSTICK ASSAYS
    • 捕获和检测用于DIPSTICK测定的形式多样性
    • US20080160516A1
    • 2008-07-03
    • US11686580
    • 2007-03-15
    • Helen LEEHsiang Yun HuMagda Anastassova Dineva
    • Helen LEEHsiang Yun HuMagda Anastassova Dineva
    • C12Q1/68
    • C12Q1/6834C12Q1/689C12Q2565/625
    • Dipsticks for testing for the presence of a target nucleic acid in a sample solution are described the dipsticks comprise a universal capture probe immobilised at a capture zone of the dipstick. The universal capture probe is capable of hybridising to a hook capture probe which is hybridised to the target nucleic acid in the sample solution. A contact end of the dipstick is contacted with the sample solution to cause hook capture probe hybridised to the target nucleic acid to move by capillary action to the capture zone where the target nucleic acid can be detected. Use of the universal and hook capture probes allows dipsticks to be prepared which can be used to capture any target nucleic acid, thereby simplifying preparation of the dipsticks. Specificity of target nucleic acid capture is then achieved by use of an appropriate hook capture probe. Methods and kits are also described.
    • 描述了用于测试样品溶液中目标核酸存在的浸渍条,浸渍条包括固定在量油尺的捕获区的通用捕获探针。 通用捕获探针能够与钩状捕获探针杂交,其与样品溶液中的靶核酸杂交。 将量油尺的接触端与样品溶液接触,使钩捕捉探针与目标核酸杂交,通过毛细管作用移动到可以检测靶核酸的捕获区。 使用通用和钩捕获探针可以制备可以用于捕获任何靶核酸的骰子,从而简化骰子的制备。 然后通过使用适当的钩捕获探针实现靶核酸捕获的特异性。 还描述了方法和试剂盒。
    • 18. 发明申请
    • SIGNAL ENHANCEMENT SYSTEM WITH MULTIPLE LABELED-MOIETIES
    • 具有多个标签的信号增强系统
    • US20120094272A1
    • 2012-04-19
    • US13165624
    • 2011-06-21
    • Helen LeeLing HuangMagda Anastassova DinevaHsiang Yun Hu
    • Helen LeeLing HuangMagda Anastassova DinevaHsiang Yun Hu
    • G01N33/53G01N33/577C12Q1/70C07K16/46C07K1/107
    • G01N33/558Y10S435/81Y10S436/807Y10S977/70Y10S977/773Y10S977/788Y10S977/918
    • Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.
    • 描述了用于检测分析物的浸渍试验。 在优选的实施方案中,能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合,并且将被认为含有分析物的测试溶液弄脏。 分析物,生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。 在捕获区存在胶体金标记表示测试溶液中存在分析物。 使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级,并且胶体金标记的抗生物素抗体被恶化 量油尺在一个单独的步骤。 还描述了用于执行本发明的测试的套件。
    • 19. 发明申请
    • SIGNAL ENHANCEMENT SYSTEM WITH MULTIPLE LABELED-MOIETIES
    • 具有多个标签的信号增强系统
    • US20080206853A1
    • 2008-08-28
    • US12042762
    • 2008-03-05
    • Helen LeeLing HuangMagda Anastassova DinevaHsiang Yun Hu
    • Helen LeeLing HuangMagda Anastassova DinevaHsiang Yun Hu
    • C12M1/00B01J19/00
    • G01N33/558Y10S435/81Y10S436/807Y10S977/70Y10S977/773Y10S977/788Y10S977/918
    • Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.
    • 描述了用于检测分析物的浸渍试验。 在优选的实施方案中,能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合,并且将被认为含有分析物的测试溶液弄脏。 分析物,生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。 在捕获区存在胶体金标记表示测试溶液中存在分析物。 使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级,并且胶体金标记的抗生物素抗体被恶化 量油尺在一个单独的步骤。 还描述了用于执行本发明的测试的套件。
    • 20. 发明授权
    • Capture and detection format versatility for dipstick assays
    • 捕捉和检测格式通用性测试仪测试
    • US07192701B2
    • 2007-03-20
    • US10332131
    • 2001-07-06
    • Helen LeeHsiang Yun HuMagda Anastassova Dineva
    • Helen LeeHsiang Yun HuMagda Anastassova Dineva
    • C12Q1/68C07H21/02C07H21/04
    • C12Q1/6834C12Q1/689C12Q2565/625
    • Dipsticks for testing for the presence of a target nucleic acid in a sample solution are described the dipsticks comprise a universal capture probe immobilised at a capture zone of the dipstick. The universal capture probe is capable of hybridising to a hook capture probe which is hybridised to the target nucleic acid in the sample solution. A contact end of the dipstick is contacted with the sample solution to cause hook capture probe hybridised to the target nucleic acid to move by capillary action to the capture zone where the target nucleic acid can be detected. Use of the universal and hook capture probes allows dipsticks to be prepared which can be used to capture any target nucleic acid, thereby simplifying preparation of the dipsticks. Specificity of target nucleic acid capture is then achieved by use of an appropriate hook capture probe. Methods and kits are also described.
    • 描述了用于测试样品溶液中目标核酸存在的浸渍条,浸渍条包括固定在量油尺的捕获区的通用捕获探针。 通用捕获探针能够与钩状捕获探针杂交,其与样品溶液中的靶核酸杂交。 将量油尺的接触端与样品溶液接触,使钩捕捉探针与目标核酸杂交,通过毛细管作用移动到可以检测靶核酸的捕获区。 使用通用和钩捕获探针可以制备可以用于捕获任何靶核酸的骰子,从而简化骰子的制备。 然后通过使用适当的钩捕获探针实现靶核酸捕获的特异性。 还描述了方法和试剂盒。