专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

11. 发明授权

US07056502B2 Recombinant aav vectors with AAV5 capsids and AAV5 vectors pseudotyped in heterologous capsids 失效
标题翻译：具有AAV5衣壳的重组aav载体和在异源衣壳中假型的AAV5载体
公开(公告)号：US07056502B2
公开(公告)日：2006-06-06
申请号：US10257961
申请日：2001-04-23
申请人： Markus Hildinger , James M. Wilson , Alberto Auricchio
发明人： Markus Hildinger , James M. Wilson , Alberto Auricchio
IPC分类号： A61K48/00 , C12N15/864
CPC分类号： C12N15/86 , A61K48/00 , C12N7/00 , C12N2750/14122 , C12N2750/14143 , C12N2750/14145 , C12N2750/14162 , C12N2830/008 , C12N2830/48
摘要： A pseudotyped rAAV is described, which contains sequences derived from AAV5. A method for producing rAAV pseudotyped with AAV5 capsid is described in which the rep proteins of the serotype or any cross-reactive serotype of the AAV ITRs are utilized. A similar method may be provided to generate a pseudotyped rAAV in which minigenes carrying AAV5 ITRs are packaged in capsids of a heterologous AAV serotype. Also provided are pharmaceutical compositions containing the pseudotyped rAAV and methods of using them for gene delivery.
摘要翻译：描述了一种假型rAAV，其包含衍生自AAV5的序列。描述了用AAV5衣壳制备rAAV假型的方法，其中使用AAV ITR的血清型或任何交叉反应性血清型的rep蛋白。可以提供类似的方法来产生假型rAAV，其中将携带AAV5 ITR的小基因包装在异源AAV血清型的衣壳中。还提供了含有假型rAAV的药物组合物及其用于基因递送的方法。

12. 发明申请

US20050154636A1 Method and system for selling and/ or distributing digital audio files 审中-公开
标题翻译：用于销售和/或分发数字音频文件的方法和系统
公开(公告)号：US20050154636A1
公开(公告)日：2005-07-14
申请号：US10707770
申请日：2004-01-11
申请人： Markus Hildinger , Michael Hildinger
发明人： Markus Hildinger , Michael Hildinger
IPC分类号： G06Q30/00 , G06F17/60
CPC分类号： G06Q30/02 , G06Q30/0277
摘要： The advent of audio compression algorithms in combination with high-speed Internet access has enabled consumers to download digital audio files such as music single tracks from the Internet within seconds to minutes. Whereas legitimate service providers offer music files for download on payment, many consumers download music files illegally at no direct cost by using file sharing or similar services. This leads to a potential loss in revenues for the creators, owners, sellers, and/or distributors of digital audio content. The present invention relates to a method and system for selling and/or distributing digital audio files comprising at least one advertisement message part in addition to at least one music entertainment part. By incorporating an advertisement message part, digital audio files can be sold at a lower price due to additional advertising revenues, which in turn will induce more consumers to legally purchase digital audio files, thus potentially increasing overall revenues for the creators, owners, sellers, and/or distributors of digital audio content.
摘要翻译：音频压缩算法与高速互联网接入的出现使消费者能够在几分钟内从互联网下载数字音频文件，例如音乐单曲。虽然合法的服务提供商提供音乐文件以进行付款下载，但许多消费者通过使用文件共享或类似服务，无需直接费用非法下载音乐文件。这导致数字音频内容的创作者，所有者，销售商和/或经销商的收入潜在的损失。本发明涉及除了至少一个音乐娱乐部分之外还包括至少一个广告消息部分的销售和/或分发数字音频文件的方法和系统。通过结合广告消息部分，由于额外的广告收入，数字音频文件可以以更低的价格出售，这反过来又将导致更多消费者合法购买数字音频文件，从而可能增加创作者，所有者，卖家，和/或数字音频内容的分发者。

13. 发明授权

US07319002B2 Method for purification of viral vectors having proteins which bind sialic acid 有权
标题翻译：用于纯化具有结合唾液酸的蛋白质的病毒载体的方法
公开(公告)号：US07319002B2
公开(公告)日：2008-01-15
申请号：US10485893
申请日：2002-08-06
申请人： James M. Wilson , Alberto Auricchio , Markus Hildinger
发明人： James M. Wilson , Alberto Auricchio , Markus Hildinger
IPC分类号： C12N15/864 , C12N7/02 , C12N1/02 , C07K1/14 , C07K1/22 , C07K17/02 , A61K48/00
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151
摘要： A method for isolating, from a mixture, a virus having a surface protein with a binding site for sialic acid is provided. The method involves contacting the mixture with mucin which has been linked to a solid support and washing the solid support to remove material from the mixture is non-specifically bound to the mucin-linked support. Thereafter, the specifically bound virus (e.g., AAV4 or AAV5) may be removed in a further washing step utilizing a concentrated slat or solution with low pH. Also described are pharmaceutical kits containing solid supports linked to mucin for use in isolating virus having a surface protein with a binding site for sialic acid, or detecting the presence of the virus in a biological sample.
摘要翻译：提供了从混合物中分离具有表达蛋白质的具有唾液酸结合位点的病毒的方法。该方法包括使已连接到固体支持物上的混合物与粘蛋白接触，并洗涤固体支持物以从混合物中除去材料是非特异性结合到与粘蛋白缔合的支持物。此后，可以在进一步的洗涤步骤中利用具有低pH的浓缩板条或溶液去除特异性结合的病毒（例如，AAV4或AAV5）。还描述了含有与粘蛋白连接的固体支持物的药物试剂盒，用于分离具有表达蛋白质的具有唾液酸结合位点的病毒，或检测生物样品中病毒的存在。

14. 发明申请

US20070042462A1 SUPER-SIZE ADENO-ASSOCIATED VIRAL VECTOR HARBORING A RECOMBINANT GENOME LARGER THAN 5.7 KB 失效
标题翻译：超大型亚洲相关病毒载体重组重组基因组大于5.7 KB
公开(公告)号：US20070042462A1
公开(公告)日：2007-02-22
申请号：US11161890
申请日：2005-08-21
申请人： Markus Hildinger
发明人： Markus Hildinger
IPC分类号： C12P21/06 , C12N7/00 , C12N15/861 , C12N5/06
CPC分类号： C12N15/86 , A61K48/00 , C12N2750/14143
摘要： Prior art teaches an effective packaging capacity for adeno-associated virus and adeno-associated viral vectors of 4.1 kb to 4.9 kb as well as a packaging limit of 5.2 kb to 5.6 kb. However, the inventor discovered that this packaging limit as well as that effective packaging capacity does not apply to all AAV serotypes: Whereas it is true that efficient packaging of AAV serotype 2 is limited to less than 5 kb, the inventor discovered that one can efficiently package more than 6 kb of genetic information into AAV capsids of other AAV serotypes, particularly into capsids of AAV serotype 5 and—to a lesser extent—into capsids of AAV serotype 7. This discovery will be useful in the context of gene therapy where large transgenes will have to be transferred such as the ABCA4 coding sequence, the Factor VIII coding sequence, the B-deleted Factor VIII coding sequence or minidystrophin genes.
摘要翻译：现有技术教导了4.1kb至4.9kb的腺相关病毒和腺相关病毒载体的有效包装能力以及5.2kb至5.6kb的包装限制。然而，发明人发现，这种包装限制以及有效的包装能力并不适用于所有AAV血清型：鉴于AAV血清型2的有效包装确实被限制在小于5kb，发明人发现可以有效地将超过6kb的遗传信息包装到其他AAV血清型的AAV衣壳中，特别是AAV血清型5的衣壳中，并且在较小程度上包装到AAV血清型7的衣壳中。该发现将在基因治疗的背景下有用，其中大的必须转移转基因，如ABCA4编码序列，因子VIII编码序列，缺失B因子VIII编码序列或微小营养不良蛋白基因。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式