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11. 发明申请

US20140206006A1 SINGLE CELL CLASSIFICATION METHOD, GENE SCREENING METHOD AND DEVICE THEREOF 审中-公开
标题翻译：单细胞分类方法，基因筛选方法及其装置
公开(公告)号：US20140206006A1
公开(公告)日：2014-07-24
申请号：US14239650
申请日：2012-08-24
申请人： Xun Xu , Li Bao , Weiming He , Yong Hou , Ye Tao
发明人： Xun Xu , Li Bao , Weiming He , Yong Hou , Ye Tao
IPC分类号： G06F19/22
CPC分类号： G16B30/00 , C12Q1/6827 , C12Q1/6869 , G16B20/00
摘要： Provided are a single cell classification method, a gene screening method and a device for implementing the method. In that, the single cell classification method includes the following steps: sequencing the whole genomes of a plurality of single cell samples from the same group, respectively, so as to obtain reads from each single cell sample; aligning the reads from each single cell sample to the sequence of a reference genome, respectively, and performing data filtering on said reads; on the basis of the filtered reads, determining a consistent genotype of each single cell sample, in which consistent genotypes of all the single cell samples constitute an SNP dataset of said group; aimed at said each single cell, on the basis of the SNP dataset of said group, determining a corresponding genotype for each cell at a site corresponding to a position in an SNP dataset of the reference genome; and selecting an SNP site associated with cell mutation, and on the basis of the genotype of said single cell at the site, classifying said single cell.
摘要翻译：提供单细胞分类方法，基因筛选方法和实施该方法的装置。其中，单细胞分类方法包括以下步骤：从同一组分别测序多个单细胞样品的全基因组，从而获得每个单细胞样品的读数; 将每个单个细胞样本的读数分别对准参考基因组的序列，并对所述读取执行数据过滤; 基于经过滤的读数，确定每个单细胞样品的一致的基因型，其中所有单细胞样品的一致基因型构成所述组的SNP数据集; 针对所述每个单细胞，基于所述组的SNP数据集，在对应于参照基因组的SNP数据集中的位置的位点处确定每个细胞的相应基因型; 并选择与细胞突变相关的SNP位点，并且基于所述单细胞在所述位点处的基因型，对所述单细胞进行分类。

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