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    • 12. 发明授权
    • Xylitol dehydrogenase-inactivated and arabinose reductase-inhibited mutant of Candida tropicalis, method of producing high-yield of xylitol using the same, and xylitol produced thereby
    • 木糖醇脱氢酶灭活和热带假丝酵母的阿拉伯糖还原酶抑制突变体,使用其产生高产量的木糖醇的方法和由此生产的木糖醇
    • US07820414B2
    • 2010-10-26
    • US11771081
    • 2007-06-29
    • Jung Hoe KimByoung Sam Ko
    • Jung Hoe KimByoung Sam Ko
    • C12P7/18C12P19/02C12N1/10
    • C12N1/16C12P7/18
    • Disclosed herein are Xylitol dehydrogenase-inactivated and arabinose reductase-inhibited mutant of Candida tropicalis, a method of producing a high yield of xylitol using the same, and xylitol produced by the method. More specifically, disclosed are a method for producing a high yield of xylitol, in which a high concentration of xylose contained in a biomass hydrolyzate is converted to xylitol using xylitol dehydrogenase-inactivated mutant of Candida tropicalis, without controlling dissolved oxygen to a low level, as well as xylitol produced according to the method. Also disclosed are a xylitol production method, in which the production of byproduct arabitol, which is produced when using a biomass as a substrate and adversely affects the yield of xylitol, is significantly reduced through the use of Candida tropicalis mutant ara-89 (KCTC 11136bp) having an inhibited activity of arabinose reductase converting arabinose to arabitol, thus increasing xylitol productivity, as well as xylitol produced by the method.
    • 本文公开了热带假丝酵母的木糖醇脱氢酶失活和阿拉伯糖还原酶抑制突变体,使用其产生高产率的木糖醇的方法,以及通过该方法生产的木糖醇。 更具体地,公开了一种生产高产率的木糖醇的方法,其中将生物质水解产物中所含的高浓度的木糖转化为木糖醇,使用热带假丝酵母的木糖醇脱氢酶灭活的突变体,而不将溶解氧控制在低水平, 以及根据该方法生产的木糖醇。 还公开了一种木糖醇生产方法,其中通过使用热带假丝酵母突变体ara-89(KCTC 11136bp)显着降低在使用生物质作为底物时产生的副产物阿拉伯糖醇的产生并且不利地影响木糖醇的产率 )具有抑制阿拉伯糖还原酶的活性,其将阿拉伯糖转化成阿糖醇,从而增加木糖醇生产率,以及通过该方法产生的木糖醇。