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11. 发明授权

US06753156B1 Homogeneous method for detecting and/or determining phosphorylating activity in a biological material 失效
标题翻译：用于检测和/或确定生物材料中磷酸化活性的均相方法
公开(公告)号：US06753156B1
公开(公告)日：2004-06-22
申请号：US09486701
申请日：2000-03-06
申请人： Gérard Mathis , Eric Trinquet , Marc Preaudat
发明人： Gérard Mathis , Eric Trinquet , Marc Preaudat
IPC分类号： G01N33573
CPC分类号： G01N33/6842 , G01N33/533 , G01N33/68 , Y10S435/975 , Y10S436/815
摘要： The invention relates to a novel homogeneous method of detecting and/or determining the phosphorylating activity of a biological material towards a substrate containing tyrosine and/or serine and/or threonine, and to a kit for carrying out this method.
摘要翻译：本发明涉及一种用于检测和/或确定生物材料朝向含有酪氨酸和/或丝氨酸和/或苏氨酸的底物的磷酸化活性的新型均相方法，以及用于实施该方法的试剂盒。

12. 发明授权

US08697372B2 Method for determining the binding of a given compound to a membrane receptor 有权
标题翻译：确定给定化合物与膜受体结合的方法
公开(公告)号：US08697372B2
公开(公告)日：2014-04-15
申请号：US13388150
申请日：2010-08-12
申请人： Eric Trinquet , Jurriaan Zwier , Gérard Mathis , Jean-Philippe Pin , Thierry Durroux
发明人： Eric Trinquet , Jurriaan Zwier , Gérard Mathis , Jean-Philippe Pin , Thierry Durroux
IPC分类号： G01N33/566 , C07K19/00 , C07K14/705
CPC分类号： G01N33/542 , G01N33/74
摘要： The invention relates to a method for determining whether a test compound binds preferentially to a membrane receptor R1 or to a membrane receptor R2, these receptors being known to be expressed on the surface of the cells in monomeric, homodimeric or heterodimeric form. This method is applied using one or two FRET partner pairs.
摘要翻译：本发明涉及一种用于确定测试化合物优先与膜受体R1或膜受体R2结合的方法，已知这些受体以单体，同二聚体或异二聚体形式在细胞表面表达。使用一个或两个FRET伙伴对应用此方法。

13. 发明授权

US08470523B2 Method for detecting intracellular interaction between biomolecules 有权
标题翻译：检测生物分子之间细胞内相互作用的方法
公开(公告)号：US08470523B2
公开(公告)日：2013-06-25
申请号：US12065706
申请日：2006-09-04
申请人： Iris Pribilla , Hervé Bazin , Sraboni Ghose , Norbert Tinel , Michel Fink , Eric Trinquet , Gérard Mathis
发明人： Iris Pribilla , Hervé Bazin , Sraboni Ghose , Norbert Tinel , Michel Fink , Eric Trinquet , Gérard Mathis
IPC分类号： C12Q1/00
CPC分类号： G01N33/542
摘要： The invention relates to a quantitative non-microscopic method of detecting intracellular interactions between biomolecules in living cells, in response to a biological or pharmacological stimulation, by a time-resolved proximate energy transfer effect between two members of a fluorescence donor/acceptor pair.
摘要翻译：本发明涉及通过荧光供体/受体对的两个成员之间的时间分辨的接近能量转移效应来检测响应于生物或药理学刺激的活细胞中生物分子之间的细胞内相互作用的定量非微观方法。

14. 发明申请

US20120115176A1 METHOD FOR DETECTING COMPOUNDS MODULATING DIMERS OF VFT DOMAIN MEMBRANE PROTEINS 有权
标题翻译：检测化合物调节VFT域膜蛋白二聚体的方法
公开(公告)号：US20120115176A1
公开(公告)日：2012-05-10
申请号：US13266222
申请日：2010-04-29
申请人： Etienne Doumazane , Jurriaan Zwier , Eric Trinquet , Jean-Phillippe Pin
发明人： Etienne Doumazane , Jurriaan Zwier , Eric Trinquet , Jean-Phillippe Pin
IPC分类号： C12Q1/02 , C12Q1/34 , C12Q1/48
CPC分类号： G01N33/542 , G01N33/9426 , G01N2333/726 , G01N2500/04
摘要： The invention relates to a method for selecting compounds having a modulating effect on the activation state of a dimer of VFT-domain proteins expressed in cell membranes present in a measuring medium, said dimer consisting of a first protein and of a second protein, said proteins being identical or different, wherein this method comprises the following steps: (a) labeling the first and second proteins in the N-terminal portion of their VFT domains with the members of a pair of FRET partners, the Förster radius (R0) of said pair being between 20 and 55 Å; (b) measuring the FRET signal in the absence and in the presence of the test compound within a predetermined time window; (c) selecting the test compound as a modulating compound if a difference in FRET signal in the absence and in the presence of test compound is measured in step (b). The invention can be used in the search for new medicaments and new taste modulators.
摘要翻译：本发明涉及一种选择对存在于测量介质中的细胞膜中表达的VFT结构域蛋白的二聚体的活化状态具有调节作用的化合物的方法，所述二聚体由第一种蛋白质和第二种蛋白质组成，所述蛋白质相同或不同，其中该方法包括以下步骤：（a）用一对FRET伴侣的成员将其VFT结构域的N末端部分中的第一和第二蛋白质标记，所述第一和第二蛋白质的所述F 对在20和55之间; （b）在预定时间窗内测试化合物不存在和存在时测量FRET信号; （c）如果在步骤（b）中测定了在不存在和存在测试化合物的情况下FRET信号的差异，则选择测试化合物作为调节化合物。本发明可用于寻找新的药物和新的调味剂。

15. 发明授权

US07998694B2 Method of revealing a biological process using a FRET measurement 有权
标题翻译：使用FRET测量揭示生物过程的方法
公开(公告)号：US07998694B2
公开(公告)日：2011-08-16
申请号：US12065210
申请日：2006-08-29
申请人： Eric Trinquet , Hervé Ansanay , Gérard Mathis
发明人： Eric Trinquet , Hervé Ansanay , Gérard Mathis
IPC分类号： G01N33/567
CPC分类号： G01N33/542 , G01N33/6872 , Y10S435/968
摘要： The present invention relates to a method of revealing a biological process using a FRET measurement, which comprises the following steps: incorporating, into a measurement medium containing a lipid membrane, a biological entity X coupled with a first member of a pair of FRET partners and a second biological entity Y coupled with the second member of the pair of FRET partners, the energy-donating member of the pair of FRET partners having a long lifetime and the members of said pair of FRET partners being located on either side of the lipid membrane; exciting the measurement medium at the excitation wavelength of the energy-donating member; and measuring the FRET signal or the variations in said signal emitted in said culture medium.
摘要翻译：本发明涉及使用FRET测定揭示生物过程的方法，其包括以下步骤：将含有脂质膜的生物体X与一对FRET伴侣的第一成员结合，与该对FRET伴侣的第二成员结合的第二生物实体Y，该对FRET伴侣中的能量供给成员具有长寿命，并且所述一对FRET伴侣的成员位于脂质膜的任一侧 ; 在能量供给构件的激发波长下激发测量介质; 以及测量FRET信号或在所述培养基中发射的所述信号的变化。

16. 发明申请

US20100086943A1 METHOD FOR THE DETECTION OF POST-TRANSLATIONAL MODIFICATIONS 有权
标题翻译：检测翻译后修改的方法
公开(公告)号：US20100086943A1
公开(公告)日：2010-04-08
申请号：US12516333
申请日：2007-11-27
申请人： Eric Trinquet , Achim Brinker , Emmanuel Claret , Gérard Mathis
发明人： Eric Trinquet , Achim Brinker , Emmanuel Claret , Gérard Mathis
IPC分类号： G01N33/53 , C12N5/00 , C12N5/071
CPC分类号： G01N33/542 , C12Q1/37 , C12Q1/48 , C12Q1/485
摘要： Method for the detection in homogeneous medium of a post-translational modification of a protein substrate catalyzed by a cell enzyme, characterized in that the post-translational modification reaction takes place in intact living cells, in that these cells comprise a heterologous expression vector coding for a fusion protein comprising the protein substrate and a first coupling domain and in that it comprises the following stages: (v) Incubation of the cells in the presence or in the absence of a compound to be tested capable of modulating the activity of said enzyme, (vi) Addition to the reaction medium of a first fluorescent compound member of a first pair of FRET partners covalently bonded to a coupling agent capable of binding specifically to the first coupling domain present on the protein substrate, (vii) Addition to the reaction medium of a second fluorescent compound member of this first pair of FRET partners, and covalently bonded to a binding domain specific to the site of the protein substrate having undergone the post-translational modification and not binding to the non-modified protein substrate, (i) Measurement of the FRET signal emitted by the sample, this signal being representative of the quantity of protein substrate having undergone said post-translational modification; and cells for the implementation of said method.
摘要翻译：用于在均匀培养基中检测由细胞酶催化的蛋白质底物的翻译后修饰的方法，其特征在于翻译后修饰反应在完整的活细胞中进行，因为这些细胞包含编码包含蛋白质底物和第一偶联结构域的融合蛋白，并且其包含以下阶段：（v）在待测化合物存在或不存在下能够调节所述酶的活性的细胞的孵育，（vi）向第一对FRET配偶体的第一荧光化合物成员的反应介质中加入共价结合到能够特异性结合蛋白质底物上存在的第一偶联结构域的偶联剂，（vii）加入到反应介质中的第一对FRET配偶体的第二荧光化合物成员，并且共价键合到特异于t位点的结合结构域已经经历了翻译后修饰并且不结合未修饰的蛋白质底物的蛋白质底物，（i）测量由样品发射的FRET信号，该信号代表已经经历所述翻译后蛋白质底物的量修改; 以及用于执行所述方法的单元。

17. 发明授权

US08361715B2 Method for suppressing a FRET signal, FRET signal suppressor agents and use in a method for multiplexing biological events 有权
标题翻译：用于抑制FRET信号，FRET信号抑制剂的方法和用于复用生物事件的方法中的应用
公开(公告)号：US08361715B2
公开(公告)日：2013-01-29
申请号：US12296790
申请日：2007-04-10
申请人： Gérard Mathis , Eric Trinquet , Patrizia Alberti , Michel Laget
发明人： Gérard Mathis , Eric Trinquet , Patrizia Alberti , Michel Laget
IPC分类号： C12Q1/68 , G01N33/53 , G01N21/76 , C12P19/54
CPC分类号： C12Q1/6818 , G01N33/542 , G01N33/582 , G01N2458/40 , C12Q2537/149 , C12Q2537/143 , C12Q2537/137
摘要： The invention relates to a method for suppressing the FRET emitted by a reaction medium containing a pair of fluorescent FRET partner conjugates specific for a biological event, characterized in that a FRET signal killer which does not disturb said biological event is introduced into this medium.
摘要翻译：本发明涉及一种抑制由含有对生物事件特异的一对荧光FRET配偶体共轭物的反应介质发射的FRET的方法，其特征在于将不干扰所述生物事件的FRET信号抑制剂引入该介质中。

18. 发明申请

US20090294691A1 Method for Improving the Detection of Fluorescence Signals During a Resonance Energy Transfer 有权
标题翻译：在共振能量转移过程中改进荧光信号检测的方法
公开(公告)号：US20090294691A1
公开(公告)日：2009-12-03
申请号：US11571331
申请日：2005-06-27
申请人： Eric Trinquet , Gerard Mathis
发明人： Eric Trinquet , Gerard Mathis
IPC分类号： G01N21/64
CPC分类号： G01N21/6428 , G01N21/6445
摘要： The invention concerns the use of the fluorescence polarization phenomenon to improve detection of fluorescent signals during a fluorescence resonance energy transfer (FRET). In particular, the invention concerns a method for improving signal/noise ratio in a FRET measurement. The invention also concerns an apparatus for measuring fluorescence following an energy transfer between a donor fluorescent compound and an acceptor fluorescence compound in a measurement medium.
摘要翻译：本发明涉及使用荧光偏振现象来改善在荧光共振能量转移（FRET）期间荧光信号的检测。特别地，本发明涉及一种在FRET测量中改善信号/噪声比的方法。本发明还涉及用于在测量介质中的供体荧光化合物和受体荧光化合物之间的能量转移之后测量荧光的装置。

19. 发明申请

US20090215106A1 METHOD FOR DETECTING INTRACELLULAR INTERACTION BETWEEN BIOMOLECULES 有权
标题翻译：用于检测生物分子之间的细胞间相互作用的方法
公开(公告)号：US20090215106A1
公开(公告)日：2009-08-27
申请号：US12065706
申请日：2006-09-04
申请人： Iris Pribilla , Herve Bazin , Sraboni Ghose , Norbert Tinel , Michel Fink , Eric Trinquet , Gerard Mathis
发明人： Iris Pribilla , Herve Bazin , Sraboni Ghose , Norbert Tinel , Michel Fink , Eric Trinquet , Gerard Mathis
IPC分类号： C12Q1/02
CPC分类号： G01N33/542
摘要： The present invention relates to a quantitative non-microscopic method of detecting intracellular interactions between biomolecules, on living cells, in response to a biological or pharmacological stimulation, by a time-resolved proximate energy transfer effect between two members of a fluorescence donor/acceptor pair.
摘要翻译：本发明涉及通过荧光供体/受体对的两个成员之间的时间分辨的接近能量转移效应来检测响应于生物或药理学刺激的生物分子，活细胞之间的细胞内相互作用的定量非微观方法。

20. 发明申请

US20090162861A1 METHOD FOR SUPPRESSING A FRET SIGNAL, FRET SIGNAL SUPPRESSOR AGENTS AND USE IN A METHOD FOR MULTIPLEXING BIOLOGICAL EVENTS 有权
标题翻译：用于抑制FRET信号，FRET信号抑制剂的方法和用于多重生物活性的方法
公开(公告)号：US20090162861A1
公开(公告)日：2009-06-25
申请号：US12296790
申请日：2007-04-10
申请人： Gérard Mathis , Eric Trinquet , Patrizia Alberti , Michel Laget
发明人： Gérard Mathis , Eric Trinquet , Patrizia Alberti , Michel Laget
IPC分类号： C12Q1/68 , G01N33/53 , C12Q1/48 , C07K2/00 , C07K14/00 , C07K16/00
CPC分类号： C12Q1/6818 , G01N33/542 , G01N33/582 , G01N2458/40 , C12Q2537/149 , C12Q2537/143 , C12Q2537/137
摘要： The invention relates to a method for suppressing the FRET emitted by a reaction medium containing a pair of fluorescent FRET partner conjugates specific for a biological event, characterized in that a FRET signal killer which does not disturb said biological event is introduced into this medium.
摘要翻译：本发明涉及一种抑制由含有对生物事件特异的一对荧光FRET配偶体共轭物的反应介质发射的FRET的方法，其特征在于将不干扰所述生物事件的FRET信号抑制剂引入该介质中。

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