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11. 发明申请

US20050032097A1 Method for processing samples containing sperm and non-sperm cells for subsequent analysis of the sperm DNA 审中-公开
标题翻译：用于处理含有精子和非精子细胞的样品用于随后分析精子DNA的方法
公开(公告)号：US20050032097A1
公开(公告)日：2005-02-10
申请号：US10870639
申请日：2004-06-17
申请人： Alex Garvin
发明人： Alex Garvin
IPC分类号： C12N15/10 , C12Q1/68 , C12N1/08
CPC分类号： C12N15/1017
摘要： In various aspects, the present invention provides novel and effective methods and kits for the isolation of sperm and sperm DNA from samples having at least one other cell type, or having the DNA of one other cell type. More specifically, a process for isolating sperm DNA from a mixture of sperm and non-sperm cells by filtration is provided. DNA from non-sperm cells is solubilized by selective lysis, and the intact sperm are retained on a filter, washed, and then treated in situ with a reducing agent to solubilize the sperm DNA. Significantly, centrifugation steps are not required, the process can be easily automated, and can be performed on many samples in parallel. The novel methods and kits are based on filtering selectively solubilized samples through filters that retain sperm by virtue of having uniform pore diameters that: are smaller than sperm, large enough to allow passage of cell debris and solubilized DNA; and are stable to pressure. The inventive methods and kits have broad utility, particularly in the forensic art.
摘要翻译：在各个方面，本发明提供了用于从具有至少一种其它细胞类型或具有另一种细胞类型的DNA的样品中分离精子和精子DNA的新型和有效的方法和试剂盒。更具体地，提供了通过过滤从精子和非精子细胞的混合物中分离精子DNA的方法。通过选择性裂解溶解非精子细胞的DNA，将完整的精子保留在过滤器上，洗涤，然后用还原剂原位处理以溶解精子DNA。重要的是，不需要离心步骤，该方法可以容易地自动化，并且可以并行地在许多样品上进行。新颖的方法和试剂盒基于通过过滤器过滤选择性溶解的样品，其通过具有均匀的孔径而保留精子，所述孔径小于精子，其足够大以允许细胞碎片和溶解的DNA通过; 并且压力稳定。本发明的方法和试剂盒具有广泛的用途，特别是在法医学领域。

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