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11. 发明授权

US5389547A Pretreating reagent, pretreatment method, assay with pretreated samples, assay kit and method of diagnosing infectious diseases 失效
标题翻译：预处理试剂，预处理方法，预处理样品测定，测定试剂盒和诊断传染病的方法
公开(公告)号：US5389547A
公开(公告)日：1995-02-14
申请号：US58306
申请日：1993-05-10
申请人： Shigenori Tanaka , Hiroshi Tamura
发明人： Shigenori Tanaka , Hiroshi Tamura
IPC分类号： C12Q1/04 , C12Q1/37 , G01N33/531 , G01N33/579 , G01N33/00
CPC分类号： G01N33/579 , Y10T436/10 , Y10T436/14 , Y10T436/143333 , Y10T436/25 , Y10T436/25125
摘要： A reagent denatures or eliminates factors interfering with biochemical reactions by simple treatment without requiring separation of any denatured product precipitate. The reagent makes it possible to assay, in particular, .beta.-glucan and endotoxin in blood-derived samples rapidly and efficiently with high sensitivity. The reagent includes a hexadimethrine compound and an alkali metal hydroxide or an alkali metal hydroxide as a main component. A method for assaying a substance specifically reacting with a Limulus reagent utilizing the reagent, an assay kit including at least the reagent and a Limulus reagent, and a method of diagnosing infectious diseases based on the results obtained by the assay method are also provided.
摘要翻译：试剂通过简单的处理变性或消除干扰生化反应的因素，而不需要分离任何变性产物沉淀物。该试剂使得可以以高灵敏度快速有效地测定血液来源的样品中的β-葡聚糖和内毒素。该试剂含有己二胺化合物和碱金属氢氧化物或碱金属氢氧化物作为主要成分。还提供了一种使用试剂测定与鲎试剂特异性反应的物质的方法，至少包含试剂和鲎试剂的测定试剂，以及基于通过测定方法获得的结果诊断感染性疾病的方法。

12. 发明授权

US5378610A Method for assaying endotoxin in serum or plasma using limulus amoebocyte lysate 失效
标题翻译：使用鲎变形细胞裂解液测定血清或血浆中的内毒素的方法
公开(公告)号：US5378610A
公开(公告)日：1995-01-03
申请号：US859398
申请日：1992-05-28
申请人： Shigenori Tanaka , Hiroshi Tamura
发明人： Shigenori Tanaka , Hiroshi Tamura
IPC分类号： G01N33/579 , C12Q1/34 , C12Q1/00 , C12Q1/02
CPC分类号： G01N33/579
摘要： The present invention has been made in order to solve the problems in conventional methods of assaying an endotoxin in a specimen such as plasma or serum by the limulus test, which requires a complicated pretreatment procedure such as centrifugation for removing denatured precipitates formed with an acid treatment. According to the assay method of the present invention, an endotoxin adsorbed by proteins, lipids and platelets can be efficiently liberated simply by adding a mixed aqueous solution having a specific composition according to the present invention without any separation procedure and thus a sample solution of good qualities can be prepared. After adding the mixed aqueous solution containing a specific surfactant, a compound having an imidazolyl group or an amino group and an alkaline earth metal salt and an alkaline metal hydroxide to the specimen, followed by addition of the limulus amoebocyte lysate components, the endotoxin can be easily, quickly and precisely assayed. In particular, gram-negative bacterial septicemia, which can be hardly diagnosed, can be quickly assayed thereby.
摘要翻译： PCT No.PCT / JP91 / 01309 Sec。 371日期：1992年5月28日 102（e）日期1992年5月28日PCT 1991年9月27日PCT公布。出版物WO92 / 06382 日期为1992年4月16日。本发明是为了解决通过鲎试验来测定血浆或血清等标本中的内毒素的常规方法中的问题，需要复杂的预处理步骤，例如离心分离用酸处理形成变性沉淀。根据本发明的测定方法，可以简单地通过添加根据本发明的具有特定组成的混合水溶液而无需任何分离程序，有效地释放被蛋白质，脂质和血小板吸附的内毒素，因此，可以有良好的样品溶液品质可以做好准备。将含有特定表面活性剂的混合水溶液，具有咪唑基或氨基的化合物和碱土金属盐和碱金属氢氧化物加入到样品中，然后加入鲎变形细胞裂解物组分，内毒素可以是容易，快速和准确地测定。特别地，可以快速测定可以很难诊断的革兰氏阴性细菌性败血症。

13. 发明授权

US5861520A Glycolipid analogs 失效
标题翻译：糖脂类似物
公开(公告)号：US5861520A
公开(公告)日：1999-01-19
申请号：US454630
申请日：1995-05-31
申请人： Seiichiro Ogawa , Hidetoshi Tsunoda , Jinichi Inokuchi
发明人： Seiichiro Ogawa , Hidetoshi Tsunoda , Jinichi Inokuchi
IPC分类号： C07C233/18 , C07C233/36 , C07C323/41 , C07C233/00
CPC分类号： C07C323/41 , C07C233/18 , C07C233/36 , C07C2101/16
摘要： The present invention provides a compound which is a glycolipid analog having a novel structure represented by the formula (1): ##STR1## wherein Z represents an imino group, an oxygen atom or a sulfur atom; m is an integer of from 3 to 12; and n is an integer of from 4 to 22; and shows a potent activity of inhibiting glycosidase and has potential physiological activities, for example, antiviral activity. The invention also provides a glycosidase inhibitor which comprises said glycolipid analog as an active ingredient.
摘要翻译：本发明提供了具有式（1）所示新结构的糖脂类似物的化合物：其中Z表示亚氨基，氧原子或硫原子; m为3〜12的整数， n为4〜22的整数。并且显示出抑制糖苷酶的有效活性并具有潜在的生理活性，例如抗病毒活性。本发明还提供一种糖苷酶抑制剂，其包含所述糖脂类似物作为活性成分。

14. 发明授权

US5718723A Artificial blood vessel and process for producing the same 失效
标题翻译：人造血管及其制造方法
公开(公告)号：US5718723A
公开(公告)日：1998-02-17
申请号：US305377
申请日：1994-09-13
申请人： Takehisa Matsuda , Nobuyuki Nakajima , Hiroyuki Kito
发明人： Takehisa Matsuda , Nobuyuki Nakajima , Hiroyuki Kito
IPC分类号： A61L27/00 , A61F2/06 , A61L27/34 , A61L27/50 , A61L33/00 , A61L33/08 , A61L33/12 , A61F2/04
CPC分类号： A61L33/124 , A61L27/34 , A61L27/507 , A61L33/08 , A61F2/06 , Y10S623/921
摘要： This invention provides a more functionalized artificial blood vessel which can be organized by independently designing its inner and outer surfaces and endowing them with respectively different biocompatibilities, as well as a process for producing the same. The artificial blood vessel comprises a tubular support having a layer of photogelled cinnamic acid-bound chondroitin sulfate coated on the inner surface thereof and a layer of photogelled coumarin-bound gelatin coated on the outer surface thereof. The process for producing the above artificial blood vessel comprises coating a layer of coumarin-bound gelatin on the outer surface of a tubular support and a layer of cinnamic acid-bound chondroitin sulfate on the inner surface of the support and irradiating each of the layers with light.
摘要翻译：本发明提供了一种更具功能化的人造血管，其可以通过独立地设计其内表面和外表面并赋予它们分别具有不同的生物相容性以及其制造方法来组织。人造血管包括管状支持体，其具有涂覆在其内表面上的光凝胶状肉桂酸结合的硫酸软骨素层和涂覆在其外表面上的光凝胶化香豆素结合明胶层。制造上述人造血管的方法包括在支撑体的内表面上在管状载体的外表面上涂布香豆素结合的明胶层，并在载体的内表面上涂布肉桂酸结合的硫酸软骨素层，并用光。

15. 发明授权

US5695948A Reagent for endotoxin assay and method for endotoxin assay using the same 失效
标题翻译：用于内毒素测定的试剂和使用其的内毒素测定方法
公开(公告)号：US5695948A
公开(公告)日：1997-12-09
申请号：US661705
申请日：1996-06-11
申请人： Shigenori Tanaka , Hiroshi Tamura , Kazuhiro Aita
发明人： Shigenori Tanaka , Hiroshi Tamura , Kazuhiro Aita
IPC分类号： G01N33/543 , G01N33/579 , C12Q1/56 , C12Q1/00 , C12Q1/34 , C12Q1/44
CPC分类号： G01N33/579 , G01N2333/811 , Y10S435/967
摘要： This invention provides (1) a reagent for endotoxin assay which comprises aprotinin and a limulus amebocyte lysate reagent, (2) a kit for endotoxin assay which comprises the limulus amebocyte lysate reagent and a reagent containing aprotinin, (3) a method for assaying endotoxin in a sample using the limulus amebocyte lysate reagent in which aprotinin is added to the lysate reagent and/or the sample, (4) a method for assaying endotoxin in a serine protease-containing sample using the limulus amebocyte lysate reagent in which the sample is allowed to contact with an aprotinin-immobilized insoluble carrier in advance of endotoxin assay, (5) a carrier for pretreating a serine protease-containing sample on which aprotinin is immobilized, (6) a method for inhibiting factor G activation in which aprotinin is added to the limulus amebocyte lysate reagent and (7) a factor G activation inhibitor which comprises aprotinin as an active ingredient. Endotoxin assay can be effected based on the factor C system reaction, without influences of factor G contained in the limulus amebocyte lysate reagent and/or serine proteases contained in samples.
摘要翻译：本发明提供（1）含有抑肽酶和鲎变形细胞裂解物试剂的内毒素试剂，（2）包含鲎变形细胞裂解物试剂和含有抑肽酶的试剂的内毒素试剂盒，（3）检测内毒素的方法在使用鲎变形细胞裂解物试剂的样品中，其中将抑肽酶加入到裂解物试剂和/或样品中，（4）使用鲎变形细胞裂解物试剂测定含丝氨酸蛋白酶样品中的内毒素的方法，其中样品是在内毒素测定之前允许与抑肽酶固定的不溶性载体接触，（5）用于预处理含有抑肽酶的丝氨酸蛋白酶的样品的载体，（6）抑制因子G激活的方法，其中加入抑肽酶到鲎变形细胞裂解物试剂和（7）包含抑肽酶作为活性成分的因子G激活抑制剂。内毒素测定可以基于因子C系统反应进行，而不受样品中包含的鲎变形细胞裂解物试剂和/或丝氨酸蛋白酶中所含因子G的影响。

16. 发明授权

US5684026A Aminocyclopentane derivative 失效
标题翻译：氨基环戊烷衍生物
公开(公告)号：US5684026A
公开(公告)日：1997-11-04
申请号：US561947
申请日：1995-11-22
申请人： Seiichiro Ogawa , Chikara Uchida , Hiroshi Kimura , Jin-ichi Inokuchi
发明人： Seiichiro Ogawa , Chikara Uchida , Hiroshi Kimura , Jin-ichi Inokuchi
IPC分类号： C07D263/52 , A01N43/76 , A61K31/42 , C07D261/20 , C07D263/62
CPC分类号： C07D263/52
摘要： The present invention provides aminocyclopentane derivatives which are saccharide analogs having extremely high .alpha.-glucosidase inhibitory effects and novel structures and expected to be usable or applicable to drugs or agricultural chemicals. An aminocyclopentane derivative represented by the formula (1), wherein R.sub.1 represents H while R.sub.2 represents CH.sub.2 OH, or R.sub.1 represents CH.sub.2 OH while R.sub.2 represents H and R.sub.3 represents a substituted or unsubstituted aryl group or an alkyl, alkenyl, alkynyl or hydroxyalkyl group having 1 to 10 carbon atoms, intermediates for the synthesis of the same, a process for producing the intermediates and a process for producing the aminocyclopentane derivative. ##STR1##
摘要翻译：本发明提供了具有非常高的α-葡糖苷酶抑制效果和新颖结构并且预期可用于或适用于药物或农药的糖类似物的氨基环戊烷衍生物。由式（1）表示的氨基环戊烷衍生物，其中R1表示H，R2表示CH 2 OH，或R 1表示CH 2 OH，而R 2表示H，R 3表示取代或未取代的芳基或烷基，烯基，炔基或羟烷基， 10个碳原子，其合成中间体，中间体的制备方法和氨基环戊烷衍生物的制备方法。

17. 发明授权

US5605806A Reagent for assaying endotoxin 失效
标题翻译：用于测定内毒素的试剂
公开(公告)号：US5605806A
公开(公告)日：1997-02-25
申请号：US427260
申请日：1995-04-24
申请人： Shigenori Tanaka , Hiroshi Tamura
发明人： Shigenori Tanaka , Hiroshi Tamura
IPC分类号： G01N33/579 , G01N33/569 , G01N33/543
CPC分类号： G01N33/579 , Y10S435/962
摘要： The present invention provides a reagent for assaying an endotoxin which comprises limulus amoebocyte lysate and an antibody to (1 .fwdarw. 3)-.beta.-D-glucan sensitive factor or a reagent which comprises a lysate substantially free from (1 .fwdarw. 3)-.beta.-D-glucan sensitive factor. The reagent of the present invention makes it possible to assay an endotoxin originating from gram-negative bacteria contained in a biological sample such as blood, urine and cerebrospinal fluid at an extremely high sensitivity without being affected by (1 .fwdarw. 3)-.beta.-D-glucan.
摘要翻译：本发明提供了一种用于测定内毒素的试剂，其包含鲎变形细胞裂解物和（1→3）-β-D-葡聚糖敏感因子的抗体或包含基本上不含（1-3） - β-D-葡聚糖敏感因子。本发明的试剂使得可以以极高的灵敏度测定来源于生物样品如血液，尿液和脑脊液中的革兰氏阴性细菌的内毒素，而不受（1-3）-β- D-葡聚糖。

18. 发明授权

US5550030A Reagent for endotoxin-specific assay 失效
标题翻译：内毒素特异性检测试剂
公开(公告)号：US5550030A
公开(公告)日：1996-08-27
申请号：US120469
申请日：1993-09-14
申请人： Shigenori Tanaka , Hiroshi Tamura , Makoto Ohki
发明人： Shigenori Tanaka , Hiroshi Tamura , Makoto Ohki
IPC分类号： G03C5/50 , G01N33/545 , G01N33/548 , G01N33/579 , G03C7/407 , C12Q1/37 , C12N11/08 , C12N11/12 , C12Q1/34
CPC分类号： G01N33/579
摘要： Disclosed are a reagent for endotoxin-specific assay which comprises an insoluble carrier having immobilized thereon at least an endotoxin-sensitive factor derived from a limulus amebocyte; a kit for endotoxin-specific assay containing said reagent and a substrate for activated factor C or a substrate for clotting enzyme; a method for assaying endotoxin comprising applying a sample solution to said reagent to cause endotoxin in the sample to react with factor C in said reagent and determining a change of a substrate; and a process for preparing said reagent which comprises physically or chemically immobilizing at least an endotoxin-sensitive factor derived from a limulus amebocyte on an insoluble carrier. Endotoxin in a sample, even turbid or colored, can be specifically assayed with ease and rapidness without the influence of a (1.fwdarw.3)-.beta.-glucan.
摘要翻译：公开了一种用于内毒素特异性测定的试剂，其包含其上固定有至少一种源自鲎变形细胞的内毒素敏感因子的不溶性载体; 含有所述试剂和活化因子C的底物或用于凝固酶的底物的内毒素特异性测定试剂盒; 一种用于测定内毒素的方法，包括将样品溶液施加到所述试剂以使样品中的内毒素与所述试剂中的因子C反应并确定底物的变化; 以及制备所述试剂的方法，其包括在不溶性载体上物理或化学地固定来自鲎变形细胞的至少一种内毒素敏感因子。样品中的内毒素，甚至浑浊或有色，可以在不受（1-> 3）-β-葡聚糖的影响的情况下容易且快速地进行特异性测定。

19. 发明授权

US5789462A Photocured crosslinked-hyaluronic acid contact lens 失效
标题翻译：光固化交联透明质酸隐形眼镜
公开(公告)号：US5789462A
公开(公告)日：1998-08-04
申请号：US712515
申请日：1996-09-13
申请人： Yoshihiro Motani , Satoshi Miyauchi
发明人： Yoshihiro Motani , Satoshi Miyauchi
IPC分类号： G02B1/04 , G02C7/04 , C08F290/10
CPC分类号： G02B1/043
摘要： A photocured crosslinked-hyaluronic acid contact lens which comprises a photocured crosslinked-hyaluronic acid derivative produced by the formation of a crosslinked cyclobutane ring by light irradiation from mutual photoreactive crosslinking groups of a photoreactive hyaluronic acid derivative in which the photoreactive crosslinking groups are linked to hyaluronic acid, wherein the photoreactive crosslinking groups are introduced into functional groups of hyaluronic acid via a spacer group, and the contact lens has a water content of 80 to 99% and shape compatibility and tissue affinity for the eyeball. A process for preparing a photocured crosslinked-hyaluronic acid contact lens which comprises molding the photoreactive hyaluronic acid derivative into a shape that fits to the eyeball, and subsequently irradiating the shaped product with light to effect crosslinking between the mutual photoreactive crosslinking groups.
摘要翻译：一种光固化交联透明质酸隐形眼镜，其包含通过光反应性交联基团的光反应性交联基团的光照射形成交联的环丁烷环而制备的光固化交联透明质酸衍生物，其中光反应性交联基团与透明质酸酸，其中光反应性交联基团通过间隔基团引入透明质酸的官能团，并且隐形眼镜具有80至99％的水含量和眼球的形状相容性和组织亲和力。一种制备光固化交联透明质酸隐形眼镜的方法，其包括将光反应性透明质酸衍生物模塑成适合于眼球的形状，随后用光照射成形产品以实现相互光反应性交联基团之间的交联。

20. 发明授权

US5763205A Producing pure chondroitinase ABC from Proteus vulgaris ATCC 6896 失效
标题翻译：从普通变形菌ATCC 6896生产纯软骨素酶ABC
公开(公告)号：US5763205A
公开(公告)日：1998-06-09
申请号：US443675
申请日：1995-05-18
申请人： Nobukazu Hashimoto , Hideo Mochizuki , Akio Hamai
发明人： Nobukazu Hashimoto , Hideo Mochizuki , Akio Hamai
IPC分类号： A61K38/43 , A61K38/00 , C12N9/16 , C12N9/20 , C12N9/88 , C12N9/44
CPC分类号： C12N9/88 , A61K38/00 , G03C2001/0471 , Y10S435/873
摘要： A crystallizable, purified chondroitinase ABC having a molecular weight of about 100,000 dalton by the measurement of the SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and the measurement by the gel permeation chromatography method, having alanine as the N-terminal amino acid and proline as the C-terminal amino acid is disclosed. A process for the purification of the crystallizable purified chondroitinase ABC comprising removing nucleic acid from an surfactant solution extract obtained from cells of chondroitinase ABC-producing microorganisms and chromatographically treating by concentration gradient elution using a weak cation exchange resin or a strong cation exchange resin is disclosed. A composition comprising a chondroitinase and serum albumin, gelatin, or a nonionic surfactant is disclosed. The enzyme is isolated from Proteus vulgaris ATCC 6896.
摘要翻译：通过SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）的测量和通过凝胶渗透色谱法测定的具有约100,000道尔顿分子量的可结晶纯化的软骨素酶ABC，其具有丙氨酸作为N-末端氨基酸和脯氨酸因为公开了C-末端氨基酸。公开了一种纯化可结晶纯化软骨素酶ABC的方法，包括从由软骨素酶ABC产生的微生物的细胞获得的表面活性剂溶液提取物中除去核酸，并使用弱阳离子交换树脂或强阳离子交换树脂进行色谱法处理。。公开了包含软骨素酶和血清白蛋白，明胶或非离子表面活性剂的组合物。该酶从普通变形杆菌ATCC 6896中分离。

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