专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

151. 发明授权

US08129594B2 Bacillus thuringiensis gene with lepidopteran activity 有权
公开(公告)号：US08129594B2
公开(公告)日：2012-03-06
申请号：US12477393
申请日：2009-06-03
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32 , C12N1/21 , C12P21/04 , A01N37/18
CPC分类号： C12N15/8286 , C07K14/32 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

152. 发明申请

US20120047607A1 Novel Bacillus thuringiensis Gene with Coleopteran Activity 有权
标题翻译：具有鞘翅目活性的新型苏云金芽孢杆菌基因
公开(公告)号：US20120047607A1
公开(公告)日：2012-02-23
申请号：US13211466
申请日：2011-08-17
申请人： André R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： André R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
IPC分类号： A01H5/00 , C12N15/63 , C12N1/21 , C12N5/10 , A01P7/04 , C07K14/325 , A01N63/02 , C12P21/02 , C12N15/82 , C12N15/32 , A01H5/10
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编码具有杀虫活性的昆虫的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其包括鞘翅目。本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，DNA构建体和转化的微生物的分离的核酸和包含实施方案的核酸的植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

153. 发明授权

US08101826B2 Expression of Cry3B insecticidal protein in plants 有权
标题翻译： Cry3B杀虫蛋白在植物中的表达
公开(公告)号：US08101826B2
公开(公告)日：2012-01-24
申请号：US12749943
申请日：2010-03-30
申请人： Charles P. Romano
发明人： Charles P. Romano
IPC分类号： A01H5/00 , C12N15/82 , C12N15/32 , C12N1/21
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The present invention discloses methods and compositions comprising a group of novel expression cassettes which provide significantly improved levels of accumulation of Coleopteran inhibitory Cry3B and Cry3B variant amino acid sequences when these are expressed in plants. The preferred embodiments of the invention provide at least up to ten fold higher levels of insect controlling protein relative to the highest levels obtained using prior compositions. In particular, transgenic maize expressing higher levels of a protein designed to exhibit increased toxicity toward Coleopteran pests deliver superior levels of insect protection and are less likely to sponsor development of populations of target insects that are resistant to the insecticidally active protein.
摘要翻译：本发明公开了包含一组新型表达盒的方法和组合物，当这些表达盒在植物中表达时，提供显着提高的鞘翅目昆虫抑制性Cry3B和Cry3B变体氨基酸序列的积累水平。本发明的优选实施方案相对于使用现有组合物获得的最高水平提供至少高达10倍的昆虫控制蛋白质水平。特别地，表达更高水平的表达更高水平的蛋白质的转基因玉米被设计为对鞘翅目害虫呈现增加的毒性提供了更高水平的昆虫保护，并且不太可能赞助对杀虫活性蛋白质具有抗性的目标昆虫种群的发育。

154. 发明授权

US08101825B2 AXMI-004, a delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-004，一种δ-内毒素基因及其使用方法
公开(公告)号：US08101825B2
公开(公告)日：2012-01-24
申请号：US12032445
申请日：2008-02-15
申请人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
发明人： Nadine Carozzi , Tracy Hargiss , Michael G. Koziel , Nicholas B. Duck , Brian Carr
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3 and 5, and the nucleotide sequences set forth in SEQ ID NOS:1, 2, and 4, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含δ-内毒素多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的δ-内毒素核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：3和5所示的氨基酸序列的核苷酸序列和SEQ ID NO：1,2和4所示的核苷酸序列的分离的核酸分子，以及作为其变体和片段。

155. 发明申请

US20110301223A1 COMPOSITIONS AND METHODS FOR INSECTICIDAL CONTROL OF STINKBUGS 有权
标题翻译： STINKBUGS的杀虫剂控制组合物和方法
公开(公告)号：US20110301223A1
公开(公告)日：2011-12-08
申请号：US13152795
申请日：2011-06-03
申请人： Karen E. Broglie , David C. Cerf , Rafael Herrmann , Albert L. Lu , Brian McGonigle , James K. Presnail
发明人： Karen E. Broglie , David C. Cerf , Rafael Herrmann , Albert L. Lu , Brian McGonigle , James K. Presnail
IPC分类号： A01N57/16 , A01P7/04 , C12N5/10 , C12N15/113 , C12N15/63
CPC分类号： C12N15/8286 , A01N57/16 , A01N65/00 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N2310/14 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini, and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当被害虫摄入时，例如五子科植物害虫或N. viridula，Acrosternum hilare，Piezodorus guildini和/或Halymorpha halys植物害虫，降低靶的表达序列在害虫中。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-292或302-304中任一项所述的各种靶多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是五角科。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

156. 发明授权

US08071736B2 Nucleic acid molecules encodng a cathepsin L-like cysteine protease 有权
标题翻译：核酸分子编码组织蛋白酶L样半胱氨酸蛋白酶
公开(公告)号：US08071736B2
公开(公告)日：2011-12-06
申请号：US12512425
申请日：2009-07-30
申请人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
IPC分类号： C07H21/04 , C12N1/20 , C12N15/63 , C12P21/00
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

157. 发明授权

US08067673B2 Methods for plant regeneration, transformation and production of insect resistant transgenic Okra 失效
标题翻译：植物再生，转化和产生抗虫转基因秋葵的方法
公开(公告)号：US08067673B2
公开(公告)日：2011-11-29
申请号：US11574007
申请日：2005-08-16
申请人： Usha Barwale Zehr , Madhavan Narendran Nair , Satish Govindrao Deole
发明人： Usha Barwale Zehr , Madhavan Narendran Nair , Satish Govindrao Deole
IPC分类号： C12N15/84 , A01H5/00 , A01H5/10 , A01H1/02
CPC分类号： C12N15/8286 , A01H4/005 , A01H4/008 , C12N15/8205 , C12N15/8207 , Y02A40/162
摘要： The present description concerns methods for regeneration of whole plant from the explants obtained from the Abelmoschus species preferably A. esculentus. In addition the present description also concerns methods for transforming okra plant, plant cells and tissues either with the use of recombinant Agrobacterium strain or by bombarding the explants with tungsten or gold particles coated with DNA sequences of interest. An efficient method to isolate embryos from imbibed seeds of okra is also described which enables the use of young meristematic cells of plumule tip for efficient regeneration and transformation of okra plants. Further, transformed okra plants, plant cells and tissues for improved agronomic/non agronomic traits and insect resistance are produced either by using marker based or marker free systems.
摘要翻译：本说明书涉及从Abelmoschus物种优选A. esculentus获得的外植体中再生整株植物的方法。此外，本说明书还涉及使用重组农杆菌菌株转化秋葵植物，植物细胞和组织的方法，或通过用感兴趣的DNA序列包被的钨或金颗粒轰击外植体。还描述了从秋葵的吸收种子中分离胚胎的有效方法，其使得能够使用胎头的幼嫩分生细胞来有效地再生和转化秋葵植物。此外，通过使用基于标记或无标记的系统产生转化的秋葵植物，植物细胞和组织以改善农学/非农学性状和昆虫抗性。

158. 发明申请

US20110289626A1 METHODS AND MATERIALS FOR CONFERRING RESISTANCE TO PESTS AND PATHOGENS OF PLANTS 失效
标题翻译：对植物和植物病原体的抵抗力的方法和材料
公开(公告)号：US20110289626A1
公开(公告)日：2011-11-24
申请号：US13171106
申请日：2011-06-28
申请人： CHARLES L. NIBLETT
发明人： CHARLES L. NIBLETT
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8286 , C12N15/8218 , C12N15/8279 , C12N15/8281 , C12N15/8282 , C12N15/8285 , Y02A40/162 , Y02A40/164
摘要： Methods and materials for conferring pest resistance to plants are provided. Plants are transformed with a silencing construct homologous to a gene of a plant pest that is essential for the survival, development, or pathogenicity of the pest. This results in the plant producing RNAi to the selected gene, which, when ingested by the pest results in silencing of the gene and a subsequent reduction of the pest's ability to harm the plant. In other embodiments, the pest's reduced ability to harm the plant is passed on to pest progeny. Methods and materials for depathogenesis of pests is also provided.
摘要翻译：提供了赋予植物抗虫性的方法和材料。用与害虫的存活，发育或致病性所必需的植物害虫基因同源的沉默构建体转化植物。这导致植物对所选择的基因产生RNAi，当被害虫摄入时，导致基因的沉默并随后降低害虫对植物的伤害能力。在其他实施方案中，害虫对害虫的损伤能力被传递给害虫后代。还提供了病虫害发病的方法和材料。

159. 发明申请

US20110288272A1 MODIFIED CRY3A TOXINS AND NUCLEIC ACID SEQUENCES CODING THEREFOR 有权
公开(公告)号：US20110288272A1
公开(公告)日：2011-11-24
申请号：US13189966
申请日：2011-07-25
申请人： Eric Chen , Cheryl Stacy
发明人： Eric Chen , Cheryl Stacy
IPC分类号： C07K14/325 , C12P21/02 , C12N5/10 , C12N15/32 , C12N15/63
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Methods for making a modified Cry3A toxin are disclosed. Such methods include the insertion of a protease recognition site that is recognized by a gut protease of a target insect, such as corn rootworm, into at least one position of a Cry3A toxin so that a modified Cry3A toxin is thus designed. The coding sequence of the modified Cry3A toxin may be transformed into a host cell and the host cell grown under conditions that allow the host cell to produce the modified Cry3A toxin. The host cell may be a plant cell and the plant may be comprised in a transgenic plant. Thus, the transgenic plant may be used to produce the modified Cry3A toxin.

160. 发明申请

US20110277180A1 Expression of Cry3B Insecticidal Protein in Plants 有权
标题翻译： Cry3B杀虫蛋白在植物中的表达
公开(公告)号：US20110277180A1
公开(公告)日：2011-11-10
申请号：US12749943
申请日：2010-03-30
申请人： Charles P. Romano
发明人： Charles P. Romano
IPC分类号： A01H1/00 , C12N15/63 , C12N5/10 , A61K36/899 , A01H5/00 , A01H5/10 , C12N15/82 , C12N15/74 , C07H21/04 , C12N1/21
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The present invention discloses methods and compositions comprising a group of novel expression cassettes which provide significantly improved levels of accumulation of Coleopteran inhibitory Cry3B and Cry3B variant amino acid sequences when these are expressed in plants. The preferred embodiments of the invention provide at least up to ten fold higher levels of insect controlling protein relative to the highest levels obtained using prior compositions. In particular, transgenic maize expressing higher levels of a protein designed to exhibit increased toxicity toward Coleopteran pests deliver superior levels of insect protection and are less likely to sponsor development of populations of target insects that are resistant to the insecticidally active protein.
摘要翻译：本发明公开了包含一组新型表达盒的方法和组合物，当这些表达盒在植物中表达时，提供显着提高的鞘翅目昆虫抑制性Cry3B和Cry3B变体氨基酸序列的积累水平。本发明的优选实施方案相对于使用现有组合物获得的最高水平提供至少高达10倍的昆虫控制蛋白质水平。特别地，表达更高水平的表达更高水平的蛋白质的转基因玉米被设计为对鞘翅目害虫呈现增加的毒性提供了更高水平的昆虫保护，并且不太可能赞助对杀虫活性蛋白质具有抗性的目标昆虫种群的发育。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式