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131. 发明申请

US20210198743A1 METHODS FOR SIMULTANEOUS AMPLIFICATION OF TARGET LOCI 有权
公开(公告)号：US20210198743A1
公开(公告)日：2021-07-01
申请号：US17196722
申请日：2021-03-09
申请人： Natera, Inc.
发明人： Joshua BABIARZ , Tudor Pompiliu CONSTANTIN , Lane A. EUBANK , George GEMELOS , Matthew Micah HILL , Huseyin Eser KIRKIZLAR , Matthew RABINOWITZ , Onur SAKARYA , Styrmir SIGURJONSSON , Bernhard ZIMMERMANN
IPC分类号： C12Q1/6883 , C12Q1/6811 , C12Q1/6848 , C12Q1/6809 , C12Q1/6851 , C12Q1/6844 , C12Q1/6869 , C12Q1/6855 , C12Q1/6874
摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

132. 发明申请

US20210071246A1 NOVEL PRIMERS AND USES THEREOF 有权
公开(公告)号：US20210071246A1
公开(公告)日：2021-03-11
申请号：US16959949
申请日：2019-01-11
申请人： Natera, Inc.
发明人： Bernhard ZIMMERMANN , Ryan SWENERTON , Scott DASHNER , Fei LU , Himanshu SETHI
IPC分类号： C12Q1/6853 , C12Q1/6858
摘要： Disclosed here is a composition comprising a primer that is (a) a loopable primer comprising a target-specific section, an adaptor section, and a stem-forming section, wherein the stem-forming section is hybridizable to a portion of the target-specific section to form a stem structure, or (b) a split primer comprising a first target-specific section, a second target-specific section, and an adaptor section positioned between the first target-specific section and the second target-specific section, or (c) a split-loopable primer comprising a first target-specific section, a second target-specific section, a stem-forming section positioned between the first target-specific section and the second target-specific section, and an adaptor section, or comprising a first adaptor section, a second adaptor section, a stem-forming section positioned between the first adaptor section and the second adaptor section, and a target-specific section. Also disclosed is a method for amplifying a target locus of interest from a template DNA, comprising at least two pre-amplification cycles using the loopable primer, the split primer and/or the split-loopable primer, wherein each amplification cycle comprises annealing the primer to the template DNA or pre-amplification product thereof and elongating the annealed primer. Further disclosed is a kit for amplifying a target locus of interest, comprising the loopable primer, the split primer, and/or the split-loopable primer.

133. 发明授权

US10894976B2 Compositions, methods, and kits for isolating nucleic acids 有权
公开(公告)号：US10894976B2
公开(公告)日：2021-01-19
申请号：US15898080
申请日：2018-02-15
申请人： Natera, Inc.
发明人： James Stray , Jason Tong , Xiaoke Wang
IPC分类号： C12Q1/6834 , C12N15/10
摘要： Disclosed here is a composition for isolating nucleic acids from a biological sample, comprising a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof. Also disclosed is a method for binding nucleic acids to a matrix, comprising: contacting the nucleic acids from a biological sample with the matrix in the presence of a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof, thereby binding the nucleic acids to the matrix. Further disclosed is a kit for isolating nucleic acids from a biological sample comprising a binding buffer, wherein the binding buffer comprises a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof.

134. 发明申请

US20210009990A1 METHODS FOR ISOLATING NUCLEIC ACIDS WITH SIZE SELECTION 有权
公开(公告)号：US20210009990A1
公开(公告)日：2021-01-14
申请号：US16969892
申请日：2019-02-15
申请人： Natera, Inc.
发明人： James STRAY , Jason TONG
IPC分类号： C12N15/10 , C12Q1/6853
摘要： Disclosed here is a method for isolating nucleic acids from a biological sample, comprising: (a) contacting a first composition comprising nucleic acids obtained from a biological sample with a first matrix under a low-stringency binding condition having less than 1% aliphatic alcohols that binds less than 5% of nucleic acids of shorter than about 118 bp and more than 30% of nucleic acids longer than about 194 bp to a first matrix; and (b) contacting a second composition comprising remainder of the first composition with a second matrix under a high-stringency binding condition having less than 1% aliphatic alcohol that binds more than 70% of nucleic acids longer than about 72 bp and 30% of nucleic acids longer than about 50 bp to the second matrix. Further disclosed is a kit for isolating nucleic acids from a biological sample, comprising (a) a first binding buffer for establishing a low-stringency binding condition having less than 1% aliphatic alcohols that binds less than 5% of nucleic acids shorter than about 118 bp and more than 30% of nucleic acids longer than about 194 bp to a matrix, and (b) a second binding buffer for establishing a high-stringency binding condition having less than 1% aliphatic alcohol that binds more than 70% of nucleic acids longer than about 72 bp and 30% of nucleic acids longer than about 50 bp to the matrix.

135. 发明授权

US10793912B2 Methods for simultaneous amplification of target loci 有权
公开(公告)号：US10793912B2
公开(公告)日：2020-10-06
申请号：US16399947
申请日：2019-04-30
申请人： Natera, Inc.
发明人： Joshua Babiarz , Tudor Popiliu Constantin , Lane A. Eubank , George Gemelos , Matthew Micah Hill , Huseyin Eser Kirkizlar , Matthew Rabinowitz , Onur Sakarya , Styrmir Sigurjonsson , Bernhard Zimmermann
IPC分类号： C12Q1/68 , C12Q1/6883 , C12Q1/6811 , C12Q1/6848 , C12Q1/6809 , C12Q1/6851 , C12Q1/6855 , C12Q1/6874
摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

136. 发明申请

US20200248266A1 METHOD OF DETECTING TUMOUR RECURRENCE 审中-公开
公开(公告)号：US20200248266A1
公开(公告)日：2020-08-06
申请号：US16347134
申请日：2017-11-01
申请人： Natera, Inc. , UCL BUSINESS PLC
发明人： Robert Charles SWANTON , Christopher ABBOSH
IPC分类号： C12Q1/6886 , C12Q1/686
摘要： The invention relates to subject-specific methods for detecting recurrence of tumours based on an understanding of the clonal/subclonal mutation profile of the subject's tumour and detection of the mutations in their cell-free DNA (cfDNA), typically by multiplex PCR of tumour mutations such as single nucleotide variants (SNVs).

137. 发明申请

US20200157629A1 METHODS FOR SIMULTANEOUS AMPLIFICATION OF TARGET LOCI 审中-公开
公开(公告)号：US20200157629A1
公开(公告)日：2020-05-21
申请号：US16777700
申请日：2020-01-30
申请人： Natera, Inc.
发明人： Joshua BABIARZ , Tudor Pompiliu CONSTANTIN , Lane A. EUBANK , George GEMELOS , Matthew Micah HILL , Huseyin Eser KIRKIZLAR , Matthew RABINOWITZ , Onur SAKARYA , Styrmir SIGURJONSSON , Bernhard ZIMMERMANN
IPC分类号： C12Q1/6883 , C12Q1/6811 , C12Q1/6848 , C12Q1/6809 , C12Q1/6851 , C12Q1/6855 , C12Q1/6874
摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

138. 发明授权

US10597723B2 Methods for simultaneous amplification of target loci 有权
公开(公告)号：US10597723B2
公开(公告)日：2020-03-24
申请号：US16288022
申请日：2019-02-27
申请人： Natera, Inc.
发明人： Joshua Babiarz , Tudor Pompiliu Constantin , Lane A. Eubank , George Gemelos , Matthew Micah Hill , Huseyin Eser Kirkizlar , Matthew Rabinowitz , Onur Sakarya , Styrmir Sigurjonsson , Bernhard Zimmermann
IPC分类号： C12Q1/68 , C12Q1/6883 , C12Q1/6811 , C12Q1/6848 , C12Q1/6855 , C12Q1/6874
摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

139. 发明授权

US10597708B2 Methods for simultaneous amplifications of target loci 有权
公开(公告)号：US10597708B2
公开(公告)日：2020-03-24
申请号：US16400023
申请日：2019-04-30
申请人： Natera, Inc.
发明人： Bernhard Zimmermann , Matthew Hill , Philippe Lacroute , Michael Dodd , Alexander Wong
IPC分类号： C12Q1/68 , C12Q1/686 , C12Q1/6874 , C12Q1/6883 , C12Q1/6811 , C12Q1/6848
摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

140. 发明授权

US10533219B2 Compositions and methods for identifying nucleic acid molecules 有权
公开(公告)号：US10533219B2
公开(公告)日：2020-01-14
申请号：US15716058
申请日：2017-09-26
申请人： Natera, Inc.
发明人： Bernhard Zimmermann , Ryan Swenerton , Matthew Rabinowitz , Styrmir Sigurjonsson , George Gemelos , Apratim Ganguly , Himanshu Sethi
IPC分类号： C12Q1/68 , C12Q1/6869 , C12Q1/6806
摘要： The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

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