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    • 115. 发明申请
    • Method of classifying counting leucocytes
    • 白细胞计数分类方法
    • US20050202400A1
    • 2005-09-15
    • US10517422
    • 2003-06-23
    • Tomohiro TsujiToshihiro MizukamiAya KonishiYusuke MoriYukie Nakazawa
    • Tomohiro TsujiToshihiro MizukamiAya KonishiYusuke MoriYukie Nakazawa
    • G01N15/00G01N15/14G01N33/52C12Q1/00
    • G01N33/52G01N2015/1477
    • There is provided a method for classifying and counting leukocytes with abnormal DNA amount, which comprises: (1) a step of staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent to lyse erythrocytes, with a fluorescent dye which can make a difference in the fluorescence intensity at least among mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes; (2) a step of introducing the sample containing the stained cells into a flow cytometer to measure scattered light and fluorescence of the respective cells; (3) a step of classifying leukocytes and coincidence cells/platelet clumps utilizing a difference in the intensity of a scattered light peak and a difference in the scattered light width; (4) a step of classifying and counting mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes, utilizing a difference in the scattered light intensity and a difference in the fluorescence intensity of leukocytes classified in the step (3).
    • 提供了一种用于对异常DNA量进行白细胞分类和计数的方法,其包括:(1)通过用溶血剂处理从血液样品获得的样品中的细胞染色红细胞裂解红细胞的步骤,可以使用荧光染料 至少在成熟白细胞,具有异常DNA量的白细胞和未成熟白细胞之间的荧光强度差异; (2)将含有染色细胞的样品引入流式细胞仪中以测量各细胞的散射光和荧光的步骤; (3)利用散射光峰的强度和散射光宽度的差异来分类白细胞和符合细胞/血小板结块的步骤; (4)利用步骤(3)中分类的散射光强度和白细胞的荧光强度差异,对成熟白细胞,具有异常DNA量的白细胞和未成熟白细胞进行分类和计数的步骤。