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101. 发明申请

US20050032238A1 Vented microfluidic separation devices and methods 审中-公开
标题翻译：通气微流控分离装置及方法
公开(公告)号：US20050032238A1
公开(公告)日：2005-02-10
申请号：US10927570
申请日：2004-08-25
申请人： Christoph Karp , Ronald Gamble
发明人： Christoph Karp , Ronald Gamble
IPC分类号： B01L3/00 , G01N30/16 , G01N30/40 , G01N30/46 , G01N30/56 , G01N30/60 , G01N30/00
CPC分类号： G01N30/466 , B01L3/5025 , B01L3/502707 , B01L3/502723 , B01L3/50273 , B01L3/502753 , B01L2300/0816 , B01L2300/0864 , B01L2300/0887 , B01L2400/0487 , G01N30/16 , G01N30/603 , G01N30/6095 , G01N2030/565 , Y10T436/25375
摘要： A pressure-driven microfluidic device for separating chemical or biological species from a sample provides an on-board stationary phase packing manifold or distribution network for simultaneously packing multiple separation channels. The packing manifold or distribution may include both a stationary phase inlet port and a vent port, and the vent port may include an associated porous material or frit. Methods for operating pressure-driven microfluidic separation devices include the steps of venting the packing manifold to an environment having a lower pressure than that present in the separation columns to allow any retained sample portions to migrate away from the separation channels, thereby minimizing or eliminating cross-talk and sample contamination.
摘要翻译：用于从样品中分离化学或生物物质的压力驱动的微流体装置提供了一种用于同时包装多个分离通道的板上固定相填充歧管或分配网络。包装歧管或分配可以包括固定相入口和通气口，并且排气口可以包括相关联的多孔材料或玻璃料。用于操作压力驱动的微流体分离装置的方法包括以下步骤：将包装歧管排放到比分离柱中存在的压力低的环境中，以允许任何保留的样品部分离开分离通道迁移，从而最小化或消除交叉 -talk和样品污染。

102. 发明申请

US20050014278A1 Acid-labile isotope-coded extractant (ALICE) and its use in quantitative mass spectrometric analysis of protein mixtures 失效
标题翻译：酸不稳定同位素编码提取物（ALICE）及其在蛋白质混合物的定量质谱分析中的应用
公开(公告)号：US20050014278A1
公开(公告)日：2005-01-20
申请号：US10919365
申请日：2004-08-17
申请人： Yongchang Qiu , Jack Wang , Rodney Hewick
发明人： Yongchang Qiu , Jack Wang , Rodney Hewick
IPC分类号： G01N27/62 , C07D405/12 , G01N1/28 , G01N30/46 , G01N30/72 , G01N30/88 , G01N33/532 , G01N33/58 , G01N33/68 , G01N33/00
CPC分类号： G01N33/6848 , C07D405/12 , G01N30/461 , G01N30/463 , G01N30/468 , G01N30/7233 , G01N33/532 , G01N33/58 , G01N33/6803 , Y10T436/10 , Y10T436/105831 , Y10T436/13 , Y10T436/24 , Y10T436/25125 , B01D15/362 , B01D15/325
摘要： The method of the invention provides novel compounds, termed acid-labile isotope-coded extractants (ALICE), for quantitative mass spectrometric analysis of protein mixtures. The compounds contain a thiol-reactive group that is used to capture cysteine-containing peptides from all peptide mixtures, an acid-labile linker, and a non-biological polymer. One of the two acid-labile linkers is isotopically labeled and therefore enables the direct quantitation of peptides/proteins through mass spectrometric analysis. Because no functional proteins are required to capture peptides, a higher percentage of organic solvent can be used to solubilize the peptides, particularly hydrophobic peptides, through the binding, washing and eluting steps, thus permitting much better recover of peptides. Moreover, since the peptides are covalently linked to the non-biological polymer (ALICE), more stringent washing is allowed in order to completely remove non-specifically bound species. Finally, peptides captured by ALICE are readily eluted from the polymer support under mild acidic condition with high yield and permit the direct down stream mass spectrometric analysis without any further sample manipulation. In combination with our novel dual column two dimensional liquid chromatography- mass spectrometry (2D-LC-MS/MS) design, the ALICE procedure proves to a general approach for quantitative mass spectrometric analysis of protein mixtures with better dynamic range and sensitivity.
摘要翻译：本发明的方法提供了新的化合物，称为酸不稳定同位素编码的提取物（ALICE），用于蛋白质混合物的定量质谱分析。该化合物含有硫醇反应性基团，其用于从所有肽混合物，酸不稳定接头和非生物聚合物捕获含半胱氨酸的肽。两个酸不稳定接头之一是同位素标记的，因此能够通过质谱分析直接定量肽/蛋白质。由于不需要功能蛋白来捕获肽，所以可以使用较高百分比的有机溶剂来通过结合，洗涤和洗脱步骤来增溶肽，特别是疏水性肽，因此可以更好地恢复肽。此外，由于肽与非生物聚合物（ALICE）共价连接，所以允许更严格的洗涤以完全除去非特异性结合的物质。最后，ALICE捕获的肽在温和的酸性条件下以高产率容易地从聚合物载体上洗脱，并允许直接的下游质谱分析，无需进一步的样品操作。结合我们的双柱二维液相色谱 - 质谱（2D-LC-MS / MS）设计，ALICE方法证明了具有更好的动态范围和灵敏度的蛋白质混合物的定量质谱分析的一般方法。

103. 发明授权

US06843918B2 Protection of the chromatographic bed in dynamic axial compression chromatography devices 失效
标题翻译：动态轴向压缩色谱装置中色谱床的保护
公开(公告)号：US06843918B2
公开(公告)日：2005-01-18
申请号：US10366689
申请日：2003-02-14
申请人： Wilhelm Hauck , Harlene Marks , Jean Blehaut , Roger Marc Nicoud
发明人： Wilhelm Hauck , Harlene Marks , Jean Blehaut , Roger Marc Nicoud
IPC分类号： G01N27/22 , B01D15/08 , B01J20/291 , G01N30/46 , G01N30/52 , G01N30/60 , G01N30/80 , G01N30/88
CPC分类号： B01D15/08 , G01N30/52 , G01N30/6004 , G01N30/6021 , G01N2030/522 , G01N30/62
摘要： The invention relates to a chromatography device comprising: a column (12) intended to house, between its two ends (16a, 16b) a chromatographic bed (14) and a fluid to be chromatographed, a fluid layer (48) being defined between a surface of the bed (14) and one (16a) of the ends of the column (12); a piston (36) sliding in the column (12), the piston (36) being one of the ends (16a, 16b) of the column (12); at least a first probe (45, 46) for detecting the surface of the bed (14), the probe (45, 46) being mounted on the end (16a) of the column (12) defining the layer (48); and means (52) for adjusting the position of the piston (36) according to the detection of the surface of the bed (14) by the probe (45, 46). The invention also relates to processes for separating at least two compounds of a fluid to be chromatographed and to methods of adjusting the position of the piston with respect to the surface of the chromatographic bed using the chromatography device.The invention has the advantage of allowing effective separation of the compounds of the fluid to be chromatographed while ensuring that the bed is protected.
摘要翻译：本发明涉及一种色谱装置，其包括：用于在其两端（16a，16b）之间容纳色谱床（14）和要进行色谱分离的流体的柱（12），流体层（48）定义在床（14）的表面和柱（12）的端部的一个（16a）;活塞（36），其在柱（12）中滑动，活塞（36）是其中一个端部（16a，16b）至少一个用于检测床（14）的表面的第一探针（45,46），探针（45,46）安装在柱（12）的端部（16a）上，所述探针（45,46）限定层（48）; 和用于通过探针（45,46）检测床（14）表面的活塞（36）的位置的装置（52）。本发明还涉及用于分离至少两种化合物待层析的流体和使用色谱装置调节活塞相对于色谱床表面的位置的方法。本发明的优点在于允许有效分离要进行色谱分离的流体的化合物，同时确保床被保护。

104. 发明授权

US6103199A Capillary electroflow apparatus and method 失效
标题翻译：毛细管电流装置及方法
公开(公告)号：US6103199A
公开(公告)日：2000-08-15
申请号：US153566
申请日：1998-09-15
申请人： Torleif Ove Bjornson , Randy M. McCormick , David S. Soane
发明人： Torleif Ove Bjornson , Randy M. McCormick , David S. Soane
IPC分类号： B01J19/00 , B01L3/00 , B01L3/02 , C40B60/14 , G01N27/447 , G01N30/28 , G01N30/46 , G01N30/60 , G01N35/00
CPC分类号： G01N27/44791 , B01J19/0046 , B01J19/0093 , B01L3/0268 , B01L3/5025 , B01L3/5027 , B01L3/50273 , G01N27/44743 , B01J2219/00317 , B01J2219/00369 , B01J2219/00783 , B01J2219/00889 , B01J2219/00891 , B01L2200/10 , B01L2300/0829 , B01L2300/0838 , B01L2300/0883 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0442 , B01L2400/0481 , C40B60/14 , G01N2030/285 , G01N2035/00237 , G01N30/466 , G01N30/6095
摘要： The present invention concerns an apparatus for conducting a microfluidic process. The apparatus comprises integral first and second plates. The first plate comprises an array of sample receiving elements for receiving a plurality of samples from an array of sample containers and dispensing the samples. The second plate comprises a planar array of microfluidic networks of cavity structures and channels for conducting a microfluidic process. Also disclosed is a method for processing an array of samples. At least a portion of each sample in an array of sample wells is simultaneously transferred to a corresponding array of microfluidic networks of cavity structures and channels by means of a corresponding array of sample receiving elements that is in integral fluid communication with the array of microfluidic networks. The samples are then processed. Also disclosed is a device for conducting a microfluidic process wherein the device comprising a planar substrate having a planar array of microfluidic networks of cavity structures and channels for conducting a microfluidic process. A plurality of such devices may be present on a continuous sheet. The invention further includes kits for carrying out microfluidic processes comprising an apparatus as described above.
摘要翻译：本发明涉及用于进行微流程的装置。该装置包括集成的第一和第二板。第一板包括用于从样品容器阵列接收多个样品并分配样品的样品接收元件阵列。第二板包括用于进行微流程的空腔结构和通道的微流体网络的平面阵列。还公开了一种用于处理样本阵列的方法。样品孔阵列中的每个样品的至少一部分通过与微流控网络阵列整体流体连通的相应的样品接收元件阵列同时转移到腔结构和通道的相应阵列的微流体网络。然后处理样品。还公开了一种用于进行微流程过程的装置，其中该装置包括具有用于进行微流程的空腔结构和通道的微流体网络的平面阵列的平面基板。多个这样的装置可以存在于连续片材上。本发明还包括用于进行微流程工艺的试剂盒，其包括如上所述的装置。

105. 发明授权

US6099654A Process for recovering betaine 失效
标题翻译：回收甜菜碱的方法
公开(公告)号：US6099654A
公开(公告)日：2000-08-08
申请号：US367690
申请日：1999-08-19
申请人： Kikuzo Kaneko , Takayuki Masuda , Fumihiko Matsuda , Kohei Sato , Kouji Tanikawa
发明人： Kikuzo Kaneko , Takayuki Masuda , Fumihiko Matsuda , Kohei Sato , Kouji Tanikawa
IPC分类号： B01D15/08 , C07C227/28 , C07C227/40 , C07C227/42 , C07C229/02 , C07C229/12 , G01N30/46 , G01N30/88 , C07B63/00
CPC分类号： C07C227/42 , C07C227/28
摘要： A starting solution material in the form of waste produced in the course of separation and recovery of sucrose from sugar beet extract or sugar beet molasses with a 2-component separation simulated moving bed chromatographic separator (using a strongly acidic cation exchange resin in a salt form as chromatographic packing) or its concentrate is used to separate therefrom a crude betaine fraction with a 2-component separation simulated moving bed chromatographic separator using as chromatographic packing a strongly acidic cation exchange resin in an ionic form equilibrated with that of the starting solution material. The crude betaine fraction or its concentrate is used to separate therefrom a betaine fraction with a 2-component separation simulated moving bed chromatographic separator using as chromatographic packing a strongly acidic cation exchange resin having at least 10% (based on the ion exchange capacity) of the ionic form thereof changed to the Ca form. The betaine fraction may be concentrated and subjected to crystallization, whereby high-purity betaine can be obtained.
摘要翻译： PCT No.PCT / JP98 / 05843 Sec。 371日期1999年8月19日 102（e）1999年8月19日PCT 1998年12月24日PCT PCT。出版物WO99 / 33784 日期1999年7月8日在使用2-组分分离模拟移动床色谱分离器（使用强酸性阳离子交换器）从甜菜提取物或甜菜糖蜜分离和回收蔗糖过程中产生的废物形式的起始溶液材料树脂为盐形式作为色谱填充）或其浓缩物用于将粗制甜菜碱部分与2-组分分离模拟移动床层析分离器分离，使用与酸性阳离子交换树脂平衡的离子形式的强酸性阳离子交换树脂起始溶液材料。使用粗制甜菜碱级分或其浓缩物将其与2-组分分离模拟移动床层析分离器的甜菜碱级分分离，使用色谱填料将具有至少10％（基于离子交换容量）的强酸性阳离子交换树脂其离子形式变为Ca形式。可以将甜菜碱级分浓缩并进行结晶，由此可以获得高纯度的甜菜碱。

106. 发明授权

US06007602A Apparatus and method for chemical modulation 失效
标题翻译：用于化学调制的装置和方法
公开(公告)号：US06007602A
公开(公告)日：1999-12-28
申请号：US51535
申请日：1998-06-12
申请人： Edward B. Ledford, Jr. , John B. Phillips
发明人： Edward B. Ledford, Jr. , John B. Phillips
IPC分类号： G01N30/02 , G01N30/30 , G01N30/46 , G01N30/60 , G01N30/88 , B01D15/08
CPC分类号： G01N30/465 , G01N30/463 , G01N2030/025 , G01N2030/8881 , G01N30/30 , G01N30/461 , G01N30/6047 , G01N30/6091
摘要： An apparatus and a method are described for forming a chemical modulation of a substance present in a fluid stream, which utilize a movable device, such as a movable heater, to induce changes in the retention of a chemical substance flowing through the modulator tube. The modulator tube has an inlet, a first portion in communication with the inlet, a second portion in communication with said first portion, and an outlet in communication with said second portion. The method involves causing a chemical modulation by moving retention alteration device relative to a modulator tube.
摘要翻译： PCT No.PCT / US96 / 16376 Sec。 371日期：1998年6月12日 102（e）1998年6月12日PCT PCT 1996年10月10日PCT公布。公开号WO97 / 13570 日期1997年4月17日描述了一种用于形成流体流中存在的物质的化学调节的装置和方法，其利用诸如可移动加热器的可移动装置来引起流过的化学物质的保留的变化调制管。调制器管具有入口，与入口连通的第一部分，与所述第一部分连通的第二部分和与所述第二部分连通的出口。该方法包括通过相对于调制器管移动保持改变装置来进行化学调节。

107. 发明授权

US6000274A Method for automatically detecting total hydrocarbon content and individual volatile organic compound concentrations of waste gas 失效
标题翻译：自动检测总烃含量和废气中各种挥发性有机化合物浓度的方法
公开(公告)号：US6000274A
公开(公告)日：1999-12-14
申请号：US012589
申请日：1998-01-23
申请人： Ching-Chih Lai , Hung-Chiao Cheng , Han-Wen Chu , Hsiang-Hsien Hsiao
发明人： Ching-Chih Lai , Hung-Chiao Cheng , Han-Wen Chu , Hsiang-Hsien Hsiao
IPC分类号： G01N30/20 , G01N30/24 , G01N30/46 , G01N30/62 , G01N30/88 , G01N33/00 , G01N30/00 , G01N33/18
CPC分类号： G01N30/20 , G01N30/24 , G01N30/468 , G01N33/0047 , G01N2030/201 , G01N2030/623 , G01N2030/8881
摘要： A method for automatically detecting waste gas. The waste gas is led into a multiple switching valve through which the waste gas is sampled. Then, the waste gas sample is conveyed to an empty column and/or a chromatographic column, via the multiple switching valve. Then, the total hydrocarbon content of the waste gas sample is measured when the waste gas sample is conveyed to the empty column, and the waste gas sample is separated for measuring individual volatile organic compound concentrations of the waste gas sample when the waste as sample is conveyed to the chromatographic column.
摘要翻译：一种自动检测废气的方法。废气被引入多个切换阀，通过该多重切换阀对废气进行采样。然后，废气样品通过多重切换阀输送到空柱和/或色谱柱。然后，当将废气样品输送到空塔时测量废气样品的总烃含量，并且当废样作为样品时废气样品分离用于测量废气样品的各种挥发性有机化合物浓度输送到色谱柱。

108. 发明授权

US5918273A Unitary one-piece sample mini-column 失效
标题翻译：一体式单件样品迷你柱
公开(公告)号：US5918273A
公开(公告)日：1999-06-29
申请号：US802511
申请日：1997-02-20
申请人： Marcus J. Horn
发明人： Marcus J. Horn
IPC分类号： B01J20/285 , B01J20/281 , B01J20/283 , B01L3/00 , B01L9/06 , G01N30/02 , G01N30/22 , G01N30/24 , G01N30/36 , G01N30/46 , G01N30/60 , G01N30/86 , G01N30/88 , G01N35/00 , G01N35/10
CPC分类号： G01N35/00732 , B01L3/5025 , B01L3/50255 , B01L3/50855 , B01L9/06 , G01N30/88 , G01N2030/027 , G01N2030/8804 , G01N2030/8831 , G01N2030/8881 , G01N2035/00752 , G01N30/24 , G01N30/36 , G01N30/466 , G01N30/6043 , Y10T436/119163
摘要： This invention is directed to a unitary one-piece sample mini-column for a cassette chemical immobilization and treatment system for enabling the performance of various complex chemistries. The cassette assembly comprises a plurality of analyte sample columns ("mini-columns"), reagent wells containing pre-packaged reagents including powdered, microencapsulated, liquid or lyophillized reagents, analyte loading funnels, alignment mechanisms for the analyte sample columns, and a machine readable instruction code set for identfying a chemical treatment protocol. The mini-columns are improved columns having a high pressure interface capability to permit direct insertion of the mini-column into a high-pressure solvent line for use as a support column for HPLC analysis. The same chemistries may be performed at all sample mini-column addresses, or a separate, completely independent set of protocols may be defined for each address, or for each block of addresses pursuant to the instructions contained in the machine readable code on the cassette.
摘要翻译：本发明涉及一种用于能够实现各种复杂化学物质的盒式化学固定和处理系统的整体式单片式样品微型柱。盒组件包括多个分析物样品柱（“微型柱”），包含预包装试剂（包括粉末状，微胶囊化的，液体或冻干试剂）的试剂孔，分析物加载漏斗，分析物样品柱的对准机构和机器用于识别化学处理协议的可读指令代码集。迷你柱是具有高压力界面能力的改进的柱，以允许将微型塔直接插入高压溶剂管线中，用作HPLC分析的支柱。可以在所有样品迷你列地址处执行相同的化学物质，或者可以根据包含在盒式机器可读代码中的指令为每个地址或每个地址块定义单独的完全独立的协议集合。

109. 发明授权

US5914025A Ion chromatographic method and apparatus using ion reflux 失效
标题翻译：离子色谱法和离子回流装置
公开(公告)号：US5914025A
公开(公告)日：1999-06-22
申请号：US781537
申请日：1997-01-09
申请人： Hamish Small
发明人： Hamish Small
IPC分类号： G01N30/02 , B01J39/26 , B01J41/20 , G01N30/34 , G01N30/46 , G01N30/60 , G01N30/96 , B01D61/48
CPC分类号： G01N30/96 , B01J39/26 , B01J41/20 , G01N30/34 , G01N2030/965 , G01N30/461
摘要： A method and apparatus for generating an acid or base eluent in an aqueous stream solely from an ion exchange bed for liquid chromatography of anions or cations and for simultaneously suppressing conductivity of the eluent in the ion exchange bed after chromatographic separation. For a system in which a base is generated for the analysis of anions by ion chromatography, the method uses a bed of cation exchange material with first and second bed sections arranged in series. For anion analysis, the method includes:(a) flowing an aqueous feed stream through the first bed section while applying an electrical potential to form an aqueous eluent stream comprising a cation hydroxide base,(b) flowing a liquid sample stream containing anions to be detected and the eluent through a chromatographic separator portion of said first bed section, to separate said anions to be detected,(c) flowing said aqueous separated anion stream through said second bed section substantially free of anion exchange material and including exchangeable hydronium ions, while applying an electrical potential to convert said base to weakly ionized form, and displacing some of said exchangeable hydronium ions with cations from said base, cations electromigrating from said second bed section to said first bed section in the opposite direction to said aqueous feed stream to replenish exchangeable cations displaced from said first bed in step (a), and(d) flowing said suppressor effluent stream past a detector in which the separated anions in said suppressor effluent are detected.
摘要翻译：一种仅在离子交换床中进行阴离子或阳离子液相色谱的含水流中产生酸或碱洗脱液的方法和装置，并同时抑制色谱分离后离子交换床中的洗脱液的电导率。对于通过离子色谱分析阴离子产生碱的系统，该方法使用具有串联排列的第一和第二床部分的阳离子交换材料床。对于阴离子分析，所述方法包括：（a）在施加电势的同时使含水进料流流过第一床部分以形成包含阳离子氢氧化物碱的含水洗脱液流，（b）使含有阴离子的液体样品流流动为检测和洗脱液通过所述第一床部分的色谱分离部分分离，以分离待检测的阴离子，（c）使所述含水分离的阴离子物流通过所述第二床部分基本上不含阴离子交换材料并且包括可交换的水合氢离子，同时施加电位以将所述碱转化为弱电离形式，以及使来自所述碱的阳离子将一些所述可交换的水合氢离子置换，所述阳离子以与所述含水进料流相反的方向从所述第二床部分电解至所述第一床部分，以补充在步骤（a）中从所述第一床排出的可交换阳离子，和（d）使所述抑制剂流出物st 通过其中检测到所述抑制流出物中分离的阴离子的检测器。

110. 发明授权

US5904749A Gas chromatographic method 失效
标题翻译：气相色谱法
公开(公告)号：US5904749A
公开(公告)日：1999-05-18
申请号：US186900
申请日：1994-01-27
申请人： Christopher Huk-Shi Chen , Shinn-Der Chang , Chengwen Weng , Yuanming Tang , Ingo Bernd Holzhueter , Samuel Hugh Hawthorne
发明人： Christopher Huk-Shi Chen , Shinn-Der Chang , Chengwen Weng , Yuanming Tang , Ingo Bernd Holzhueter , Samuel Hugh Hawthorne
IPC分类号： B01D59/26 , G01N30/02 , G01N30/12 , G01N30/16 , G01N30/30 , G01N30/46 , G01N30/54 , G01N30/80 , B01D15/08
CPC分类号： G01N30/461 , B01D59/26 , G01N30/16 , G01N30/30 , G01N2030/025 , G01N2030/122 , G01N2030/165 , G01N2030/3015 , G01N2030/3023
摘要： A gas chromatographic method utilizes columns having either ascending or descending temperature profiles to effect separation even under column overloading conditions. A fixed ascending temperature profile column allows for good separation of components of a mixture under conditions of column overloading. A mixture to be separated is chromatographed on at least one such column prior to chromatography on a column maintained with a descending temperature profile. This latter column has surprisingly been found to greatly reduce tailing of less volatile species. The invention also includes a variable temperature descending column chromatography to provide an initial ordering of a large quantity of components prior to chomatography on the fixed ascending temperature profile column, or on the serial fixed ascending and descending temperature profile pair of columns.
摘要翻译：气相色谱法利用具有上升或下降温度曲线的色谱柱，即使在色谱柱过载条件下也能进行分离。固定的上升温度曲线柱允许在柱过载的条件下良好分离混合物的组分。待分离的混合物在至少一个这样的色谱柱上进行色谱分离，然后色谱法在保持下降温度曲线的色谱柱上进行。令人惊讶地发现后一列极大地减少了挥发性较低的物种的拖尾。本发明还包括可变温度降序柱色谱，以在固定升温温度曲线柱上的色谱法之前或在串联固定的升序和降序温度曲线对对上提供大量组分的初始排序。

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