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101. 发明申请

US20050005332A1 Plants having high plant map values 有权
标题翻译：植物植物地图值高
公开(公告)号：US20050005332A1
公开(公告)日：2005-01-06
申请号：US10909860
申请日：2004-08-02
申请人： Karen Fincher , Stanislaw Flasinski , Jack Wilkinson
发明人： Karen Fincher , Stanislaw Flasinski , Jack Wilkinson
IPC分类号： A01H5/00 , C12N15/09 , C12N15/82 , C07H21/04 , C12N15/87 , C12N15/63
CPC分类号： C12N15/8275 , A01N57/20 , C12N7/00 , C12N9/1092 , C12N15/8216 , C12N15/8221 , C12N15/8222 , C12N15/8223 , C12N15/823 , C12N15/8261 , C12N15/8286 , C12N2710/00043 , Y02A40/146 , Y02A40/162
摘要： The present invention relates to novel plant expression constructs. More specifically the present invention provides DNA constructs comprising 5′ regulatory sequences for modulating the expression of operably linked genes in plants.
摘要翻译：本发明涉及新型植物表达构建体。更具体地，本发明提供了包含用于调节植物中可操作连接的基因的表达的5'调节序列的DNA构建体。

102. 发明授权

US06793927B1 Construction of Pasteurella haemolytica vaccines 有权
标题翻译：建立溶血性巴氏杆菌疫苗
公开(公告)号：US06793927B1
公开(公告)日：2004-09-21
申请号：US09210747
申请日：1998-12-15
申请人： Robert E. Briggs , Fred M. Tatum
发明人： Robert E. Briggs , Fred M. Tatum
IPC分类号： A61K39102
CPC分类号： C12N9/1092 , A61K39/102 , C12N9/1007 , C12N9/22 , C12N15/102 , C12N15/74
摘要： Methylation of DNA can be a critical step in the introduction of DNA into P. haemolytica. A methyltransferase has been isolated and molecularly cloned for this purpose. Use of the methyltransferase has allowed construction of defined, attenuated mutants for use as vaccines to protect cattle.
摘要翻译： DNA的甲基化可能是将DNA引入溶血杆菌中的关键步骤。为此目的已经分离并分子克隆了甲基转移酶。使用甲基转移酶已经允许构建定义的，减毒的突变体，用作疫苗以保护牛。

103. 发明申请

US20040082770A1 Novel glyphosate N-acetyltransferase (GAT) genes 失效
标题翻译：新型草甘膦N-乙酰转移酶（GAT）基因
公开(公告)号：US20040082770A1
公开(公告)日：2004-04-29
申请号：US10427692
申请日：2003-04-30
申请人： Verdia, Inc. , Pioneer Hi-Bred International, Inc.
发明人： Linda A. Castle , Dan Siehl , Lorraine Giver , Jeremy Minshull , Cristina Ivy , Yong Hong Chen , Phillip A. Patten , Rebecca Gorton , Nicholas B. Duck , Billy Fred McCutchen , Roger Kemble
IPC分类号： C12Q001/68 , C07H021/04 , C12N009/10 , C12N005/04
CPC分类号： C12N15/8275 , C12N9/1029 , C12N9/1092 , C12N15/8209 , C12Y205/01019
摘要： Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.
摘要翻译：本文提供了新的蛋白质，包括能够催化草甘膦和其他结构相关蛋白质的乙酰化的蛋白质。还提供了能够编码这些蛋白质的新型多核苷酸，包含这些新蛋白质和/或多核苷酸中的一种或多种的组合物，包含这些新化合物的重组细胞和转基因植物，涉及新化合物的多样化方法，以及使用该化合物的方法。本文提供的一些新颖的方法和化合物可以用于产生对草甘膦有抗性的生物，如植物。

104. 发明授权

US06566587B1 Mutated 5-enolpyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene 失效
标题翻译：突变的5-烯醇丙酮酸莽草酸-3-磷酸合酶，编码所述蛋白质的基因和含有所述基因的转化植物
公开(公告)号：US06566587B1
公开(公告)日：2003-05-20
申请号：US08945144
申请日：1998-01-20
申请人： Michel Lebrun , Alain Sailland , Georges Freyssinet , Eric Degryse
发明人： Michel Lebrun , Alain Sailland , Georges Freyssinet , Eric Degryse
IPC分类号： C12N1582
CPC分类号： C12N9/1092 , C12N15/8275
摘要： The present invention relates to DNA molecules of plant origin encoding a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme wherein the a first EPSPS coding sequence that normally encodes a threonine residue of a mature EPSPS sequence is modified to encode isoleucine of the mature EPSPS sequence, and a second EPSPS coding sequence that normally encodes a proline residue of a mature EPSPS sequence is modified to encode serine of the mature EPSPS sequence, wherein the first and second residues are respectively located at relatively positions 102 and 106 of a mature EPSPS sequence encoded by said DNA molecule; and the production of a transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The mutated enzyme, which substantially maintains the catalytic activity of the wild-type enzyme, allows for increased tolerance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells.
摘要翻译：本发明涉及编码经修饰的5-烯醇丙酮酸莽草酸-3-磷酸合成酶（EPSPS）酶的植物来源的DNA分子，其中通常将编码成熟EPSPS序列的苏氨酸残基的第一EPSPS编码序列修饰为编码修饰成熟的EPSPS序列，并且通常编码成熟EPSPS序列的脯氨酸残基的第二EPSPS编码序列被修饰以编码成熟EPSPS序列的丝氨酸，其中第一和第二残基分别位于成熟的EPSPS序列的相对位置102和106 由所述DNA分子编码的EPSPS序列; 以及产生对膦酰基甲基甘氨酸家族例如草甘膦的除草剂具有抗性或耐受性的转基因植物。基本上保持野生型酶的催化活性的突变酶允许植物对膦酰基甲基甘氨酸家族的除草剂的耐受性或耐受性增加，并允许植物，其器官的基本正常生长或发育，组织或细胞。

105. 发明申请

US20030084473A1 Non-transgenic herbicide resistant plants 审中-公开
标题翻译：非转基因除草剂抗性植物
公开(公告)号：US20030084473A1
公开(公告)日：2003-05-01
申请号：US10214766
申请日：2002-08-09
申请人： VALIGEN
发明人： Greg Gocal , Patricia Avissar , Mark Knuth , Peter Beetham , Keith Walker
IPC分类号： A01H001/00 , C12N009/10 , C12N015/82 , C12N005/04
CPC分类号： C12N15/8274 , C12N9/1092 , C12N15/8275
摘要： The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. The present invention also relates to a non-transgenic plant cell in which the EPSPS gene has been mutated, a non-transgenic plant regenerated therefrom, as well as a plant resulting from a cross using a regenerated non-transgenic plant having a mutated EPSPS gene. The amino acids at the positions 126, 177, 207, 438, 479, 480 and/or 505 are changed tp produce a mutant EPSPS gene product.
摘要翻译：本发明涉及对膦酰甲基甘氨酸家族例如草甘膦的除草剂具有抗性或耐受性的非转基因植物的生产。本发明还涉及重组型寡核苷酸在编码5-烯醇丙酮酸莽草酸-3-磷酸合成酶（EPSPS）的基因中的植物的染色体或附加型序列中进行所需的突变的用途。基本上保持野生型蛋白质的催化活性的突变蛋白质允许植物对膦酰基甲基甘氨酸家族除草剂的抗性或耐受性增加，并允许植物，其器官的基本正常生长或发育，组织或细胞，与野生型植物相比，无论是否存在除草剂。本发明还涉及其中EPSPS基因已被突变的非转基因植物细胞，从其再生的非转基因植物以及由使用具有突变的EPSPS基因的再生非转基因植物的交叉产生的植物。位置126,177,207,438,479,480和/或505处的氨基酸被改变tp产生突变体EPSPS基因产物。

106. 发明申请

US20020168680A1 GLYPHOSATE-TOLERANT 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASES 失效
标题翻译：甘油三酯5-磷酸三磷酸三磷酸酯合成酶
公开(公告)号：US20020168680A1
公开(公告)日：2002-11-14
申请号：US09464099
申请日：1999-12-16
发明人： GERARD FRANCIS BARRY , GANESH MURPHY KISHORE , STEPHEN ROGERS PADGETTE , WILLIAM CARLTON STALLINGS
IPC分类号： G01N033/53 , C07K016/00 , C12P021/08 , C12N001/00
CPC分类号： C12N9/1092 , C12N15/8275 , Y10S435/816 , Y10S435/822 , Y10S435/839 , Y10S435/874 , Y10S435/883 , Y10T436/143333
摘要： Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.
摘要翻译：公开了编码II类EPSPS酶的基因。这些基因可用于产生耐草甘膦除草剂的转化细菌和植物。 II类EPSPS基因与已知的I类EPSPS基因几乎没有同源性，并且不与I类EPSPS的探针杂交。 II类EPSPS酶的特征在于在草甘膦存在下比I类EPSPS更具动力学效力。还公开了用II类EPSPS基因转化的植物以及在种植的转基因作物田中选择性控制杂草的方法。

107. 发明申请

US20020062503A1 Glyphosate tolerant wheat plant 33391 and compositions and methods for detection thereof 有权
标题翻译：草甘膦耐受性小麦植物33391及其检测方法
公开(公告)号：US20020062503A1
公开(公告)日：2002-05-23
申请号：US09682597
申请日：2001-09-25
发明人： Guilan Chen , Catherine M. Hironaka , Hua-ping Zhou
IPC分类号： C12N015/82 , A01H005/00 , A01H005/10 , C12N015/10 , C12N015/11
CPC分类号： C12N9/1092 , C12N15/8275 , C12Y205/01019
摘要： The present invention provides a DNA construct composition that relates to transgenic glyphosate tolerant wheat plants. The invention relates to the wheat plant 33391, the progeny thereof and to methods for the detection of wheat plant 33391 and its progeny and compositions thereof.
摘要翻译：本发明提供了涉及转基因草甘膦耐受小麦植物的DNA构建组合物。本发明涉及小麦植物33391，其后代以及检测小麦植物33391及其后代及其组合物的方法。

108. 发明授权

US06362396B1 Chimeric gene for the transformation of plants 失效
标题翻译：用于植物转化的嵌合基因
公开(公告)号：US06362396B1
公开(公告)日：2002-03-26
申请号：US09013520
申请日：1998-01-26
申请人： Nicole Chaubet , Claude Gigot , Georges Freyssinet , Bernard Leroux , Michel Lebrun , Alain Sailland
发明人： Nicole Chaubet , Claude Gigot , Georges Freyssinet , Bernard Leroux , Michel Lebrun , Alain Sailland
IPC分类号： A01H500
CPC分类号： C12N9/1092 , C12N15/8274
摘要： 1) Chimeric gone for conferring to plants an increased tolerance to a herbicide. 2) It comprises, in the direction of transcription, a promoter region, a transit peptide region, a sequence encoding glyphosate tolerance and a polyadenylation signal region, wherein the promoter region consists of at least one promoter of a plant histone gene enabling the expression of the herbicide tolerance protein in the regions of glyphosate accumulation. 3) Production of glyphosate-tolerant plants.
摘要翻译： 1）嵌合去除赋予植物增加对除草剂的耐受性。 2）其在转录方向包含启动子区，转运肽区，编码草甘膦耐受的序列和多聚腺苷酸化信号区，其中启动子区由植物组蛋白基因的至少一个启动子组成，使得能够表达草甘膦积累区域的除草剂耐受性蛋白。 3）耐草甘膦植物的生产。

109. 发明授权

US06338961B1 Isolated DNA sequence capable of serving as regulatory element in a chimeric gene which can be used for the transformation of plants 失效
标题翻译：分离的DNA序列能够作为可用于植物转化的嵌合基因中的调节元件
公开(公告)号：US06338961B1
公开(公告)日：2002-01-15
申请号：US09000062
申请日：1998-05-29
申请人： Richard DeRose , Nicole Chaubet , Claude Gigot
发明人： Richard DeRose , Nicole Chaubet , Claude Gigot
IPC分类号： C07H2102
CPC分类号： C12N9/1092 , A01K2217/05 , C07K14/415 , C12N15/8216 , C12N15/8222 , C12N15/8223 , C12N15/8229 , C12N15/8231 , C12N15/8233 , C12N15/8275
摘要： An isolated DNA sequence capable of serving as regulatory element in a chimeric gene which can be used for the transformation of plants is disclosed. A chimeric gene for the transformation of plants is also disclosed. The gene comprises at least, in the direction of transcription, a promoter sequence, a transgene and a regulatory element, characterized in that the regulatory element consists of at least one intron 1 in the noncoding 5′ region of a plant histone gene allowing the expression of the protein in the zones and undergoing rapid growth. The production of transgenic plants is also disclosed.
摘要翻译：公开了能够用作植物转化的嵌合基因中作为调节元件的分离的DNA序列。还公开了用于转化植物的嵌合基因。该基因至少在转录方向上包含启动子序列，转基因和调节元件，其特征在于调节元件由植物组蛋白基因的非编码5'区域中的至少一个内含子1组成，允许表达的蛋白质在区域内并经历快速生长。还公开了转基因植物的生产。

110. 发明授权

US06248876B1 Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases 失效
标题翻译：耐草甘膦5-烯醇丙酮酸莽草酸-3-磷酸合成酶
公开(公告)号：US06248876B1
公开(公告)日：2001-06-19
申请号：US09137440
申请日：1998-08-20
申请人： Gerard Francis Barry , Ganesh Murthy Kishore , Stephen Rogers Padgette , William Carlton Stallings
发明人： Gerard Francis Barry , Ganesh Murthy Kishore , Stephen Rogers Padgette , William Carlton Stallings
IPC分类号： C07H2104
CPC分类号： C12N9/1092 , C12N15/8275 , Y10S435/816 , Y10S435/822 , Y10S435/839 , Y10S435/874 , Y10S435/883 , Y10T436/143333
摘要： Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.
摘要翻译：公开了编码II类EPSPS酶的基因。这些基因可用于产生耐草甘膦除草剂的转化细菌和植物。 II类EPSPS基因与已知的I类EPSPS基因几乎没有同源性，并且不与I类EPSPS的探针杂交。 II类EPSPS酶的特征在于在草甘膦存在下比I类EPSPS更具动力学效力。还公开了用II类EPSPS基因转化的植物以及在种植的转基因作物田中选择性控制杂草的方法。

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