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101. 发明授权

US06020138A Methods for detecting nucleic acids by ligation of multiple oligomers 有权
标题翻译：通过连接多个寡聚体来检测核酸的方法
公开(公告)号：US06020138A
公开(公告)日：2000-02-01
申请号：US241979
申请日：1999-02-02
申请人： Hashem Akhavan-Tafti
发明人： Hashem Akhavan-Tafti
IPC分类号： C12N15/10 , C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12N15/10 , C12Q1/6827
摘要： Methods of synthesizing polynucleotides are disclosed involving the simultaneous ligation of a set of oligomer 5'-phosphates onto a template-bound primer. The set of these oligomers can be preselected to contain oligomers which are complementary to the template strand or the oligomers can be supplied as a library and allowed to self select. The synthesis by ligation can proceed unidirectionally or bidirectionally from the primer and can be used to synthesize both strands simultaneously by the use of two primers. The ligation is preferably performed with a ligase enzyme. The methods of synthesis are useful in a variety of applications, including cloning, amplification, labeling, diagnostic assays, mutation analysis and screening, gene expression monitoring and sequence analysis.
摘要翻译：公开了合成多核苷酸的方法，其涉及将一组低聚物5'-磷酸酯同时连接到模板结合的引物上。这些低聚物的一组可以预选为含有与模板链互补的寡聚物，或者可以将寡聚物作为文库提供并允许自我选择。通过连接的合成可以从引物单向或双向进行，并且可以通过使用两种引物同时合成两条链。连接优选用连接酶进行。合成方法可用于各种应用，包括克隆，扩增，标记，诊断测定，突变分析和筛选，基因表达监测和序列分析。

102. 发明授权

US05998175A Methods of synthesizing and amplifying polynucleotides by ligation of multiple oligomers 失效
标题翻译：通过多个寡聚体的连接合成和扩增多核苷酸的方法
公开(公告)号：US05998175A
公开(公告)日：1999-12-07
申请号：US121887
申请日：1998-07-24
申请人： Hashem Akhavan-Tafti
发明人： Hashem Akhavan-Tafti
IPC分类号： C12N15/10 , C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12N15/10 , C12Q1/6827
摘要： Methods of synthesizing polynucleotides are disclosed involving the simultaneous ligation of a set of oligomer 5'-phosphates onto a template-bound primer. The set of these oligomers can be preselected to contain oligomers which are complementary to the template strand or the oligomers can be supplied as a library and allowed to self select. The synthesis by ligation can proceed unidirectionally or bidirectionally from the primer and can be used to synthesize both strands simultaneously by the use of two primers. The ligation is preferably performed with a ligase enzyme. The methods of synthesis are useful in a variety of applications, including cloning, amplification, labeling, diagnostic assays, mutation analysis and screening, gene expression monitoring and sequence analysis.
摘要翻译：公开了合成多核苷酸的方法，其涉及将一组低聚物5'-磷酸酯同时连接到模板结合的引物上。这些低聚物的一组可以预选为含有与模板链互补的寡聚物，或者可以将寡聚物作为文库提供并允许自我选择。通过连接的合成可以从引物单向或双向进行，并且可以通过使用两种引物同时合成两条链。连接优选用连接酶进行。合成方法可用于各种应用，包括克隆，扩增，标记，诊断测定，突变分析和筛选，基因表达监测和序列分析。

103. 发明授权

US5866045A Water soluble tri-substituted 1,2-dioxetane compounds and assay compositions having increased storage stability 失效
标题翻译：水溶性三取代的1,2-二氧杂环丁烷化合物和具有增加的储存稳定性的测定组合物
公开(公告)号：US5866045A
公开(公告)日：1999-02-02
申请号：US896150
申请日：1997-07-17
申请人： Hashem Akhavan-Tafti , Zahra Arghavani , Renuka DeSilva , Kumar Thakur
发明人： Hashem Akhavan-Tafti , Zahra Arghavani , Renuka DeSilva , Kumar Thakur
IPC分类号： G01N33/535 , C07C69/00 , C07D305/08 , C07D321/00 , C07F9/12 , C07F9/655 , C09K11/06 , C09K11/07 , G01N21/76 , G01N33/53 , G01N33/543 , G01N33/58 , C09K3/00
CPC分类号： C07C69/734 , C07C67/343 , C07D321/00 , C07F9/12 , C07F9/65512 , G01N33/5308 , G01N33/54326 , G01N33/582 , C07C2103/74
摘要： Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility and storage stability are provided. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure and an additional fluorine atom or lower alkyl group provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. These dioxetanes display substantially improved stability on storage. Compositions comprising these dioxetanes, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.
摘要翻译：提供了具有改善的水溶性和储存稳定性的稳定的，酶促触发的化学发光1,2-二氧杂环丁烷。二氧杂环丁烷进一步被设置在二氧杂环丁烷结构上的水溶性基团和另外的氟原子或低级烷基取代，当用于胶囊化学分析系统的测定中时，通过消除试剂残留的问题而提供优异的性能。这些二氧杂环丁烷在储存时显示出显着提高的稳定性。还提供了包含这些二氧杂环丁烷的组合物，非聚合阳离子表面活性剂增强剂和任选的荧光剂，用于提供增强的化学发光。

104. 发明授权

US5843666A Chemiluminescent detection methods using dual enzyer-labeled binding partners 失效
标题翻译：使用双酶标记结合配偶体的化学发光检测方法
公开(公告)号：US5843666A
公开(公告)日：1998-12-01
申请号：US749595
申请日：1996-11-15
申请人： Hashem Akhavan-Tafti , Katsuaki Sugioka , Yumiko Sugioka , Lekkala V. Reddy
发明人： Hashem Akhavan-Tafti , Katsuaki Sugioka , Yumiko Sugioka , Lekkala V. Reddy
IPC分类号： G01N33/535 , C07D219/02 , C07D219/04 , C07D219/06 , C09B15/00 , C09K11/07 , C12Q1/28 , C12Q1/34 , C12Q1/68 , G01N21/78 , G01N33/53 , G01N33/543 , G01N33/58
CPC分类号： C07D219/02 , C07D219/04 , C07D219/06 , C09B15/00 , C12Q1/28 , G01N33/581 , G01N33/582
摘要： Methods of detecting analytes or target species using two enzyme-labeled specific binding partners where the two enzymes function in concert to produce a detectable chemiluminescent signal are disclosed. The methods use a specific binding partner labeled with a hydrolytic enzyme to produce a phenolic enhancer in close proximity to a peroxidase-labeled second specific binding partner. The method is useful to detect and quantitate with improved specificity various biological molecules including antigens and antibodies by the technique of immunoassay, proteins by Western blotting, DNA by Southern blotting, RNA by Northern blotting. The method may also be used to detect DNA mutations and juxtaposed gene segments in chromosomal translocations and particularly to unambiguously identify heterozygous genotypes in a single test.
摘要翻译：公开了使用两种酶标记的特异性结合配偶体检测分析物或靶物种的方法，其中两种酶一致地产生可检测的化学发光信号。该方法使用标记有水解酶的特异性结合配偶体以产生邻近过氧化物酶标记的第二特异性结合配偶体的酚增强剂。该方法通过免疫测定技术，蛋白质免疫分析技术，Southern印迹DNA，Northern印迹法检测和定量检测和定量检测和定量，包括抗原和抗体在内的各种生物分子。该方法还可用于检测染色体易位中的DNA突变和并置基因区段，特别是在单次测试中明确地鉴定杂合基因型。

105. 发明授权

US5686258A Chemiluminescent detection of hydrolytic enzymes using an acridan 失效
标题翻译：使用吖啶的化学发光检测水解酶
公开(公告)号：US5686258A
公开(公告)日：1997-11-11
申请号：US300367
申请日：1994-09-02
申请人： Hashem Akhavan-Tafti , Zahra Arghavani , Renuka DeSilva
发明人： Hashem Akhavan-Tafti , Zahra Arghavani , Renuka DeSilva
IPC分类号： G01N33/535 , C07D219/02 , C07D219/04 , C07D219/06 , C09B15/00 , C09K11/07 , C12Q1/28 , C12Q1/34 , G01N21/78 , G01N33/543 , G01N33/58
CPC分类号： C07D219/02 , C07D219/04 , C07D219/06 , C09B15/00 , C12Q1/28 , G01N33/581 , G01N33/582 , Y10S435/966 , Y10S435/968 , Y10S435/975
摘要： A chemiluminescent assay method, compositions and kits are described which use a protected phenolic enhancer compound which is deprotected by a hydrolytic enzyme and then enhances a chemiluminescent reaction. The reaction involves an acridan compound, preferably a derivative of an N-alkylacridan-9-carboxylic acid, which is activated to produce light by a peroxide compound and a peroxidase enzyme in the presence of the deprotected enhancer. The result is enhanced generation of light from the reaction. The hydrolytic enzyme is present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the hydrolytic enzyme is bound to a reporter molecule.
摘要翻译：描述了化学发光测定方法，组合物和试剂盒，其使用被水解酶脱保护，然后增强化学发光反应的受保护的酚类增强剂化合物。反应包括吖啶化合物，优选N-烷基吖啶-9-羧酸的衍生物，其在去保护的增强子存在下被过氧化物化合物和过氧化物酶活化以产生光。结果是增强了反应产生的光。水解酶在免疫测定，DNA探针测定或其他水解酶与报道分子结合的其他测定中单独存在或连接到特异性结合对的成员。

106. 发明授权

US5670644A Acridan compounds 失效
标题翻译：吖啶化合物
公开(公告)号：US5670644A
公开(公告)日：1997-09-23
申请号：US647383
申请日：1996-05-09
申请人： Hashem Akhavan-Tafti , Zahra Arghavani , Renuka DeSilva
发明人： Hashem Akhavan-Tafti , Zahra Arghavani , Renuka DeSilva
IPC分类号： C07D219/02 , C07D219/04 , C07D219/06 , C12Q1/28 , C07D285/38 , C07D295/00 , G01N33/532 , G01N33/533
CPC分类号： C07D219/02 , C07D219/04 , C07D219/06 , C12Q1/28
摘要： A chemiluminescent assay method, compositions, kits and chemiluminescent acridan compounds are described which use a two-step chemiluminescent reaction process. The reaction involves an acridan compound, preferably a derivative of an N-alkylacridan-9-carboxylic acid, which undergoes a reaction with a peroxide compound, a peroxidase enzyme and an enhancer under conditions of time, temperature and pH which permit the accumulation of an intermediate compound, which is subsequently induced to produce a burst of light by raising the pH. The result is generation of very high intensity light from the reaction. The peroxidase enzyme is present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the hydrolytic enzyme is bound to a reporter molecule. The method is particularly amenable to automated assays because of the separation of the incubation and light generating steps.
摘要翻译：描述了使用两步化学发光反应过程的化学发光测定方法，组合物，试剂盒和化学发光的吖啶化合物。该反应涉及吖啶化合物，优选N-烷基吖啶-9-羧酸的衍生物，其在时间，温度和pH条件下与过氧化物，过氧化物酶和增强剂发生反应，这允许积聚中间体化合物，其随后通过提高pH而被诱导产生突发性的光。结果是从反应产生非常高强度的光。过氧化物酶在免疫测定，DNA探针测定或其他测定中单独存在或连接到特异性结合对的成员，其中水解酶与报道分子结合。由于孵育和光生成步骤的分离，该方法特别适用于自动化测定。

107. 发明授权

US5552298A Enzyme-catalyzed chemiluminescence from hydroxyaryl cyclic diacylhydrazide compounds 失效
标题翻译：来自羟基芳基环酰基二酰肼化合物的酶催化化学发光
公开(公告)号：US5552298A
公开(公告)日：1996-09-03
申请号：US965231
申请日：1992-10-23
申请人： Hashem Akhavan-Tafti
发明人： Hashem Akhavan-Tafti
IPC分类号： C12Q1/28 , C12Q1/68 , G01N33/58 , G01N33/53
CPC分类号： G01N33/581 , C12Q1/28 , C12Q1/6816 , Y10S435/966 , Y10S435/968 , Y10S436/819
摘要： An assay method, compositions and test kits using a hydroxyaryl cyclic diacylhydrazide is described. A hydrogen peroxide and peroxidase enzyme. The preferred compositions incorporate enhancer compounds and a chelating agent which suppresses light production prior to addition of a peroxidase enzyme. The assay method can test for a peroxidase enzyme, a peroxide or can be used in immunoassays and probe assays.
摘要翻译：描述了使用羟基芳基环二酰基酰肼的测定方法，组合物和测试试剂盒。过氧化氢和过氧化物酶。优选的组合物掺入增强剂化合物和在加入过氧化物酶之前抑制光产生的螯合剂。测定方法可以测试过氧化物酶，过氧化物或可用于免疫测定和探针测定。

108. 发明授权

US5474725A Polymeric phosphonium sales providing enhanced chemiluminescence from 1, 2-dioxetanes 失效
标题翻译：聚合物鏻销售提供1,2-二氧杂环丁烷增强的化学发光
公开(公告)号：US5474725A
公开(公告)日：1995-12-12
申请号：US195439
申请日：1994-02-14
申请人： Hashem Akhavan-Tafti
发明人： Hashem Akhavan-Tafti
IPC分类号： C09K11/07 , C08F8/40 , C08F30/02 , C08F230/02 , C08K5/156 , C08L43/00 , C08L43/02 , G01N21/76 , G01N33/533 , G01N33/58 , C09K3/00 , C08C19/24
CPC分类号： G01N33/582 , G01N21/76 , G01N33/533
摘要： Polymeric phosphonium salts useful for enhancing the chemiluminescence produced by 1,2-dioxetanes are described. The polymers are preferably water soluble and used in immunoassays and nucleic acid assays with enzyme triggerable 1,2-dioxetanes.
摘要翻译：描述了可用于增强由1,2-二氧杂环丁烷产生的化学发光的聚合鏻盐。聚合物优选是水溶性的，并且用于具有酶触发性1,2-二氧杂环丁烷的免疫测定和核酸测定中。

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