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91. 发明申请

US20050196768A1 Monomeric and dimeric fluorescent protein variants and methods for making same 有权
标题翻译：单体和二聚体荧光蛋白变体及其制备方法
公开(公告)号：US20050196768A1
公开(公告)日：2005-09-08
申请号：US10931304
申请日：2004-08-30
申请人： Robert Campbell , Nathan Shaner , Roger Tsien
发明人： Robert Campbell , Nathan Shaner , Roger Tsien
IPC分类号： C07K14/435 , C12Q1/25 , G01N33/58 , G01N33/84 , C12Q1/68 , C07H21/04 , C12N5/06 , C12N15/09
CPC分类号： C07K14/43595 , C12Q1/25 , G01N33/585 , G01N33/84 , G01N2500/02
摘要： The present invention relates generally to fluorescent proteins and fluorescent protein variants, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomeric structures. In a further aspect, the present invention provides variants of fluorescent proteins, the variants being characterized by more efficient maturation than corresponding fluorescent proteins from which they are derived. The invention also relates to methods of making and using such fluorescent proteins and fluorescent protein variants, including fluorescent protein monomers and dimers.
摘要翻译：本发明一般涉及荧光蛋白和荧光蛋白变体，更具体地涉及Anthozoan荧光蛋白的单体和二聚体形式。在一个方面，本发明提供荧光蛋白的变体，其中变体具有降低的四聚体倾向并形成二聚体或单体结构。在另一方面，本发明提供荧光蛋白的变体，所述变体的特征在于比它们衍生的相应荧光蛋白更有效的成熟。本发明还涉及制备和使用这些荧光蛋白和荧光蛋白变体（包括荧光蛋白单体和二聚体）的方法。

92. 发明申请

US20050180994A1 Methods and compositions for the identification of anti-poxvirus agents 有权
标题翻译：用于鉴定抗痘病毒剂的方法和组合物
公开(公告)号：US20050180994A1
公开(公告)日：2005-08-18
申请号：US10975285
申请日：2004-10-27
申请人： Henry Lu , Jianing Huang , Donald Payan
发明人： Henry Lu , Jianing Huang , Donald Payan
IPC分类号： A01N61/00 , A61K39/275 , C12P21/00 , C12Q1/25 , C12Q1/37 , C12Q1/48 , C12Q1/70 , G01N20060101
CPC分类号： C12Q1/25 , G01N2333/065 , G01N2333/9015 , G01N2510/00
摘要： The invention provides methods for identifying an anti-poxviral agents. In many embodiments, the methods involve contacting a poxviral p28 polypeptide with a candidate agent, and determining an effect of the agent on a ubiquitin ligase activity of the p28 polypeptide. The effect of the agent may be determined using a variety of different cell based or biochemical assays, such as polyubiquitylation assays and cell viability assays. The invention also provides methods for modulating poxvirus pathogenicity in a cell, and methods of treating an individual infected with a poxvirus. The subject methods find use in a variety of drug discovery, research and military applications.
摘要翻译：本发明提供鉴定抗痘病毒药物的方法。在许多实施方案中，所述方法包括使痘病毒p28多肽与候选试剂接触，并确定试剂对p28多肽的泛素连接酶活性的影响。可以使用多种不同的基于细胞或生物化学测定法，例如多泛素化测定和细胞活力测定来确定试剂的作用。本发明还提供调节细胞中痘病毒致病性的方法，以及治疗感染痘病毒的个体的方法。主题方法可用于各种药物发现，研究和军事应用。

93. 发明授权

US06930180B2 Chemically modified proteins with a carbohydrate moiety 失效
标题翻译：具有碳水化合物部分的化学修饰的蛋白质
公开(公告)号：US06930180B2
公开(公告)日：2005-08-16
申请号：US10062970
申请日：2002-02-01
申请人： J. Bryan Jones , Benjamin G. Davis
发明人： J. Bryan Jones , Benjamin G. Davis
IPC分类号： C12N15/09 , C07K14/00 , C12N9/54 , C12N9/96 , C12Q1/25 , C12Q1/37 , C07G3/00
CPC分类号： B82Y5/00 , C12N9/54
摘要： The present invention relates to a chemically modified mutant protein including a cysteine residue substituted for a residue other than cysteine in a precursor protein, the substituted cysteine residue being subsequently modified by reacting the cysteine residue with a glycosylated thiosulfonate. Also, a method of producing the chemically modified mutant protein is provided. The present invention also relates to a glycosylated methanethiosulfonate. Another aspect of the present invention is a method of modifying the functional characteristics of a protein including providing a protein and reacting the protein with a glycosylated methanethiosulfonate reagent under conditions effective to produce a glycoprotein with altered functional characteristics as compared to the protein. In addition, the present invention relates to methods of determining the structure-function relationships of chemically modified mutant proteins.
摘要翻译：本发明涉及一种化学修饰的突变蛋白，包括在前体蛋白中取代半胱氨酸以外的残基的半胱氨酸残基，随后通过半胱氨酸残基与糖基化硫代磺酸酯反应来修饰取代的半胱氨酸残基。此外，提供了一种生产化学修饰的突变蛋白的方法。本发明还涉及糖基化甲磺酸盐。本发明的另一方面是一种改变蛋白质的功能特征的方法，包括提供蛋白质并使蛋白质与糖基化甲磺酸盐试剂在与蛋白质相比产生具有改变的功能特征的糖蛋白的条件下反应。此外，本发明涉及确定化学修饰的突变蛋白的结构 - 功能关系的方法。

94. 发明授权

US06890752B2 Synthases 有权
标题翻译：合成酶
公开(公告)号：US06890752B2
公开(公告)日：2005-05-10
申请号：US09893820
申请日：2001-06-28
申请人： Joseph Chappell , Kathleen R. Manna , Joseph P. Noel , Courtney M. Starks
发明人： Joseph Chappell , Kathleen R. Manna , Joseph P. Noel , Courtney M. Starks
IPC分类号： A01H5/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/10 , C12N9/12 , C12N9/88 , C12N15/09 , C12N15/82 , C12Q1/25 , C12R1/91 , G01N33/68 , C12N5/00
CPC分类号： C12N9/1085 , C12N9/00 , C12N9/88 , C12N15/8243
摘要： Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e.g., the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase. with or without a substrate bound in the active site.
摘要翻译：本文公开了新型合酶和编码此类合酶的相应核酸。这种合酶具有活性位点口袋，其包括被修饰以产生所需萜类化合物中间体和产物的关键氨基酸残基。基于例如烟草5-epi-aristolochene合酶的三维坐标设计合成酶修饰。有或没有底物结合在活性部位。

95. 发明申请

US20050064531A1 Methods for screening and identifying compounds 失效
标题翻译：筛选和鉴定化合物的方法
公开(公告)号：US20050064531A1
公开(公告)日：2005-03-24
申请号：US10911242
申请日：2004-08-04
申请人： James Landro , David Osterman , Walter Pickett
发明人： James Landro , David Osterman , Walter Pickett
IPC分类号： C12Q20060101 , C12Q1/00 , C12Q1/25 , C12Q1/26 , C12Q1/48 , G01N33/53
CPC分类号： C12Q1/25 , C12Q1/48 , G01N2500/00
摘要： The present invention relates to methods for assessing enzyme activity utilizing a phase partition system. In addition, the present invention also relates to methods for screening and identifying compounds that may be used, for example, for the treatment of diabetes, diabetes-related disorders, obesity, cardiovascular disease, cancer, and other diseases or disorders, using this phase partition system.
摘要翻译：本发明涉及利用相分配系统评估酶活性的方法。此外，本发明还涉及用于筛选和鉴定可用于例如用于治疗糖尿病，糖尿病相关疾病，肥胖症，心血管疾病，癌症和其它疾病或病症的化合物的方法，使用该相分区系统

96. 发明申请

US20050059803A1 Immunosuppressant target proteins 审中-公开
标题翻译：免疫抑制剂靶蛋白
公开(公告)号：US20050059803A1
公开(公告)日：2005-03-17
申请号：US10877320
申请日：2004-06-24
申请人： Vivian Berlin , Maria Chiu , Guillaume Cottarel , Veronique Damagnez
发明人： Vivian Berlin , Maria Chiu , Guillaume Cottarel , Veronique Damagnez
IPC分类号： G01N33/53 , C07K14/40 , C07K14/47 , C07K16/18 , C12N1/19 , C12N1/21 , C12N9/00 , C12N9/12 , C12N15/09 , C12N15/12 , C12N15/31 , C12N15/62 , C12N15/81 , C12P21/02 , C12Q1/25 , C12Q1/68 , G01N33/566 , G01N33/68 , G01N33/94 , C07K14/74
CPC分类号： G01N33/5091 , A01K2217/05 , C07K14/40 , C07K14/4702 , C07K2319/00 , C12N9/1205 , C12N9/93 , C12Q1/25 , C12Q1/6883 , C12Q1/6897 , C12Q2600/158 , G01N33/5005 , G01N33/5008 , G01N33/5011 , G01N33/502 , G01N33/68 , G01N33/6845 , G01N33/9493 , G01N2500/00
摘要： The present invention relates to the discovery of novel proteins of mammalian origin which are immediate downstream targets for FKBP/rapamycin complexes.
摘要翻译：本发明涉及哺乳动物来源的新型蛋白质的发现，其是FKBP /雷帕霉素复合物的即时下游靶标。

97. 发明申请

US20050059058A1 Compositions and methods for screening antimicrobials 失效
标题翻译：筛选抗菌药物的组合物和方法
公开(公告)号：US20050059058A1
公开(公告)日：2005-03-17
申请号：US10901897
申请日：2004-07-29
申请人： Piet de Boer , Cynthia Hale
发明人： Piet de Boer , Cynthia Hale
IPC分类号： C12N15/09 , C07K5/097 , C07K5/10 , C07K7/06 , C07K7/08 , C07K14/245 , C07K19/00 , C12N15/00 , C12N15/31 , C12Q1/25 , C12R1/19 , G01N33/15 , G01N33/53 , G01N33/94 , C12Q1/68 , C07H21/04 , G01N33/554 , G01N33/569
CPC分类号： C07K14/245 , G01N33/9446 , Y10S530/81 , Y10S530/811 , Y10S530/814 , Y10S530/817 , Y10S530/825 , Y10S930/28
摘要： A method for screening compounds for antimicrobial activity is described that utilizes bacterial protein-protein binding in vitro. The method may be performed using immobilized elements and the immobilization may be carried out using a variety of immobilization means (e.g., columns, beads, adsorbents, nitrocellulose paper, etc.) in order to screen large libraries of compounds.
摘要翻译：描述了筛选化合物用于抗微生物活性的方法，其在体外利用细菌蛋白质 - 蛋白质结合。该方法可以使用固定化元件进行，并且可以使用各种固定装置（例如，柱，珠，吸附剂，硝化纤维素纸等）进行固定以筛选大量化合物文库。

98. 发明申请

US20050054022A1 Methods and compositions comprising FATP5 for use in the diagnosis and treatment of metabolic disorders 审中-公开
标题翻译：包含用于诊断和治疗代谢紊乱的FATP5的方法和组合物
公开(公告)号：US20050054022A1
公开(公告)日：2005-03-10
申请号：US10919608
申请日：2004-08-17
申请人： Ruth Gimeno , Brian Hubbard
发明人： Ruth Gimeno , Brian Hubbard
IPC分类号： C12N20060101 , C12Q1/25 , G01N33/53 , G01N33/537 , G01N33/543 , G01N33/68 , G01N33/92
CPC分类号： G01N33/6893 , C12Q1/25 , G01N33/92 , G01N2333/9015 , G01N2500/04 , G01N2800/042 , G01N2800/044
摘要： The present invention provides methods for the identification of agents, e.g., therapeutic agents, that inhibit Fatty Acid Transport 5 (FATP5) activity, and methods of treating diseases or conditions associated with FATP5 function, e.g., obesity, insulin resistance, type 2 diabetes, dyslipidemia, fatty liver disease, and cardiovascular disease. Further aspects of the invention provide a transgenic FATP5 non-human knockout mammal, e.g., mouse, useful for elucidating the function of FATP5 in intact animals whose genomes comprise a wild-type FATP5 gene.
摘要翻译：本发明提供了用于鉴定抑制脂肪酸转运5（FATP5）活性的药剂，例如治疗剂的方法，以及治疗与FATP5功能相关的疾病或病症的方法，例如肥胖，胰岛素抵抗，2型糖尿病，血脂异常，脂肪肝疾病和心血管疾病。本发明的其它方面提供了转基因FATP5非人敲除哺乳动物（例如小鼠），其用于阐明在其基因组包含野生型FATP5基因的完整动物中FATP5的功能。

99. 发明申请

US20050032085A1 Novel chromophores/fluorophores and methods for using the same 失效
标题翻译：新型发色团/荧光团及其使用方法
公开(公告)号：US20050032085A1
公开(公告)日：2005-02-10
申请号：US10757356
申请日：2004-01-13
申请人： Yulii Labas , Nadezda Gurskaya , Yuriy Yanushevich , Arcady Fradkov , Konstantin Lukyanov , Sergey Lukyanov , Mikhail Matz
发明人： Yulii Labas , Nadezda Gurskaya , Yuriy Yanushevich , Arcady Fradkov , Konstantin Lukyanov , Sergey Lukyanov , Mikhail Matz
IPC分类号： A01K67/027 , C07H21/04 , C07K14/435 , C07K16/18 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12P21/02 , C12Q1/25 , G01N33/58 , C12Q1/68 , G01N33/53
CPC分类号： C07K14/43595
摘要： Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, as well as the proteins encoded the same, are provided. The proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from Anthozoan non-Pennatulacean (sea pen) species. Specific proteins of interest include the following specific proteins: hcriGFP; dendGFP; zoanRFP; scubGFP1; scubGFP2; rfloRFP; rfloGFP; mcavRFP; mcavGFP; cgigGFP; afraGFP; rfloGFP2; mcavGFP2; and mannFP. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.
摘要翻译：提供了编码新色氨酸/荧光素蛋白及其突变体的核酸组合物，以及编码它们的蛋白质。感兴趣的蛋白质是着色和/或荧光的蛋白质，其特征来自蛋白质的两个或多个残基的相互作用。主题蛋白的进一步特征在于它们是从非生物发光的Cnidarian（例如Anthozoan）物种获得的，或者获自Anthozoan non-Pennatulacean（海笔）物种。感兴趣的特异性蛋白质包括以下特异性蛋白质：hcriGFP; dendGFP; 动物园 scubGFP1; scubGFP2; rfloRFP; rfloGFP; mcavRFP; mcavGFP; cgigGFP; afGGFP; rfloGFP2; mcavGFP2; 和甘草苷。还感兴趣的是与上述特定蛋白质基本相似或突变的蛋白质。还提供了核酸的片段和由其编码的肽，以及针对受试者蛋白质和转基因细胞和生物体的抗体。主题蛋白质和核酸组合物可用于各种不同的应用。最后，提供了用于此类应用的试剂盒，例如包括本发明的核酸组合物。

100. 发明授权

US06844148B1 Alzheimer's disease secretase, APP substrates therefor, and uses therefor 失效
标题翻译：阿尔茨海默病分泌酶，APP底物及其用途
公开(公告)号：US06844148B1
公开(公告)日：2005-01-18
申请号：US09668314
申请日：2000-09-22
申请人： Mark Gurney , Michael Jerome Bienkowski
发明人： Mark Gurney , Michael Jerome Bienkowski
IPC分类号： A61P25/28 , C07K14/47 , C12N1/21 , C12N9/64 , C12N15/57 , C12Q1/37 , C12Q1/00 , C07K14/00 , C12Q1/25
CPC分类号： C12N9/6478 , C07K14/4711 , C07K2319/00 , C12N2799/026 , C12Q1/37 , G01N2333/96472
摘要： The present invention provides the enzyme and enzymatic procedures for cleaving the β secretase cleavage site of the APP protein and associated nucleic acids, peptides, vectors, cells and cell isolates and assays. An enzyme that cleaves the α-secretase site of APP also is provided. The invention further provides a modified APP protein and associated nucleic acids, peptides, vectors, cells, and cell isolates, and assays that are particularly useful for identifying candidate therapeutics for treatment or prevention of Alzheimer's disease.
摘要翻译：本发明提供用于切割APP蛋白和相关核酸，肽，载体，细胞和细胞分离物和测定的β分泌酶切割位点的酶和酶程序。还提供了切割APP的α-分泌酶位点的酶。本发明还提供了修饰的APP蛋白和相关核酸，肽，载体，细胞和细胞分离物以及特别可用于鉴定用于治疗或预防阿尔茨海默病的候选治疗剂的测定。

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