专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

91. 发明申请

US20080318867A1 Virulent Phages to Control Listeria Monocytogenes in Foodstuffs and in Food Processing Plants 有权
标题翻译：有毒的噬菌体在食品和食品加工厂控制李斯特菌单核细胞增多
公开(公告)号：US20080318867A1
公开(公告)日：2008-12-25
申请号：US12200375
申请日：2008-08-28
申请人： Martin LOESSNER , Richard M. Carlton
发明人： Martin LOESSNER , Richard M. Carlton
IPC分类号： A61K38/00 , C12Q1/70 , C07K14/00 , A23L3/3463
CPC分类号： C07K14/005 , A23G9/30 , A23G9/305 , A23L3/34635 , A23L3/3571 , C12N7/00 , C12N9/14 , C12N9/503 , C12N15/74 , C12N2795/10122 , C12N2795/10143
摘要： The present invention relates to virulent (lytic) Listeria monocytogenes phage from the Myoviridae family, preferably P100, alone or in combination with other virulent phages. P100 and the endolysin from P100 can be administered to food products, to the components that will be added to food products, and/or to the infrastructure of the food processing plants within which such food products are processed, or the containers or wraps in which such foods are stored and/or shipped, in order to reduce Listeria monocytogenes contamination. P100 can also be used in the present invention to identify Listeria monocytogenes bacteria present on (or within) foodstuffs, as well as those Listeria monocytogenes bacteria present in the equipment or the general environment of the food processing plants in which the foodstuffs are being processed and in animals infected with Listeria monocytogenes. The phage and the endolysin of the present invention can also be used to treat animals infected with Listeria monocytogenes. P100 will kill the bacteria that are within its host range with great efficiency and will propagate to high titer thereon. P100 can be combined with other lytic phage, and/or with other antimicrobial agents to reduce or eliminate Listeria.
摘要翻译：本发明涉及来自病毒科（Myoviridae）的毒性（溶解性）单核细胞增生利斯特氏菌（Listeria monocytogenes）噬菌体，优选P100，单独或与其它有毒噬菌体组合。 P100和来自P100的细胞内溶素可以施用于食品，将被添加到食品中的组分和/或对这些食品加工的食品加工厂的基础设施，或其中的容器或包装这些食物被储存和/或运输，以便减少单核细胞增生利斯特氏菌的污染。本发明中也可以使用P100来鉴别存在于食品中（或内部）的单核细胞增生李斯特细菌，以及存在于食品加工厂的设备或一般环境中的单核细胞增生李斯特菌，其中正在处理食品，在感染单核细胞增多性李斯特菌的动物体内。本发明的噬菌体和细胞内溶素也可用于治疗感染单核细胞增多性李斯特菌的动物。 P100将以很高的效率杀死其主体范围内的细菌，并在其上传播高滴度。 P100可以与其他溶解性噬菌体和/或与其他抗微生物剂组合以减少或消除李斯特菌。

92. 发明授权

US07438901B2 Virulent phages to control Listeria monocytogenes in foodstuffs and in food processing plants 有权
标题翻译：有毒的噬菌体在食品和食品加工厂中控制单核细胞增多性李斯特菌
公开(公告)号：US07438901B2
公开(公告)日：2008-10-21
申请号：US10516507
申请日：2003-07-07
申请人： Martin Loessner , Richard M. Carlton
发明人： Martin Loessner , Richard M. Carlton
IPC分类号： A01N63/00
CPC分类号： C07K14/005 , A23G9/30 , A23G9/305 , A23L3/34635 , A23L3/3571 , C12N7/00 , C12N9/14 , C12N9/503 , C12N15/74 , C12N2795/10122 , C12N2795/10143
摘要： The present invention relates to virulent (lytic) Listeria monocytogenes phage from the Myoviridae family, preferably P100, alone or in combination with other virulent phages. P100 and the endolysin from P100 can be administered to food products, to the components that will be added to food products, and/or to the infrastructure of the food processing plants within which such food products are processed, or the containers or wraps in which such foods are stored and/or shipped, in order to reduce Listeria monocytogenes contamination. P100 can also be used in the present invention to identify Listeria monocytogenes bacteria present on (or within) foodstuffs, as well as those Listeria monocytogenes bacteria present in the equipment or the general environment of the food processing plants in which the foodstuffs are being processed and in animals infected with Listeria monocytogenes. The phage and the endolysin of the present invention can also be used to treat animals infected with Listeria monocytogenes. P100 will kill the bacteria that are within its host range with great efficiency and will propagate to high titer thereon. P100 can be combined with other lytic phage, and/or with other antimicrobial agents to reduce or eliminate Listeria.
摘要翻译：本发明涉及来自病毒科（Myoviridae）的毒性（溶解性）单核细胞增生利斯特氏菌（Listeria monocytogenes）噬菌体，优选P100，单独或与其它有毒噬菌体组合。 P100和来自P100的细胞内溶素可以施用于食品，将被添加到食品中的组分和/或对这些食品加工的食品加工厂的基础设施，或其中的容器或包装这些食物被储存和/或运输，以便减少单核细胞增生利斯特氏菌的污染。本发明中也可以使用P100来鉴别存在于食品中（或内部）的单核细胞增生李斯特细菌，以及存在于食品加工厂的设备或一般环境中的单核细胞增生李斯特菌，其中正在处理食品，在感染单核细胞增多性李斯特菌的动物体内。本发明的噬菌体和细胞内溶素也可用于治疗感染单核细胞增多性李斯特菌的动物。 P100将以很高的效率杀死其主体范围内的细菌，并在其上传播高滴度。 P100可以与其他溶解性噬菌体和/或与其他抗微生物剂组合以减少或消除李斯特菌。

93. 发明授权

US07416847B1 In vitro peptide or protein expression library 失效
标题翻译：体外肽或蛋白质表达文库
公开(公告)号：US07416847B1
公开(公告)日：2008-08-26
申请号：US09331808
申请日：1998-02-18
申请人： Bjorn H. Lindqvist , David Andrews , Elizabeth Haggard-Ljungquist , Morten Isaksen
发明人： Bjorn H. Lindqvist , David Andrews , Elizabeth Haggard-Ljungquist , Morten Isaksen
IPC分类号： G01N33/53 , C40B30/04
CPC分类号： C07K14/005 , C12N15/1075 , C12N2795/10122
摘要： The present invention relates to methods of producing peptide or protein expression libraries. In such a library, a population of nucleotide sequences is expressed. The resulting peptides or proteins encoded by those nucleotide sequences are then screened to identify those peptides or proteins having a desired property such as the ability to bind a selected ligand. In the construction and screening of such libraries, it is desired to ensure that the nucleotide encoding a particular protein remains connected in some way with that protein so that once a desired protein having a selected property has been isolated, its encoding nucleotide sequence may be specifically recovered for subsequent manipulation for example using PCR or sequencing. The present invention makes use of a property not previously described in constructing an expression library namely the use of proteins which covalently bind DNA. DNA is expressed in such a way that the protein so expressed binds to its own encoding DNA therefore allowing the DNA to be associated only with its encoding protein.
摘要翻译：本发明涉及产生肽或蛋白质表达文库的方法。在这样的文库中，表达了核苷酸序列的群体。然后筛选由所述核苷酸序列编码的所得肽或蛋白质以鉴定具有所需性质的那些肽或蛋白质，例如结合所选择的配体的能力。在这样的文库的构建和筛选中，希望确保编码特定蛋白的核苷酸以某种方式与该蛋白质相连，使得一旦已经分离了具有选定性质的所需蛋白质，其编码核苷酸序列可以是特异性的回收用于随后的操作，例如使用PCR或测序。本发明利用以前在构建表达文库中所述的性质，即使用共价结合DNA的蛋白质。以这样的方式表达DNA，使得如此表达的蛋白质与其自身的编码DNA结合，从而允许DNA与其编码蛋白质相关联。

94. 发明申请

US20060257889A1 Ribonuclease resistant RNA preparation and utilization 审中-公开
公开(公告)号：US20060257889A1
公开(公告)日：2006-11-16
申请号：US11316340
申请日：2005-12-21
申请人： Brittan Pasloske , Dwight DuBois , David Brown , Matthew Winkler
发明人： Brittan Pasloske , Dwight DuBois , David Brown , Matthew Winkler
IPC分类号： C12Q1/68
CPC分类号： C12N7/00 , C07K14/005 , C12N15/10 , C12N15/86 , C12N15/88 , C12N2740/16022 , C12N2740/16062 , C12N2740/16222 , C12N2770/00022 , C12N2770/00023 , C12N2770/00042 , C12N2770/34022 , C12N2770/34023 , C12N2770/34042 , C12N2770/36123 , C12N2770/36143 , C12N2795/10122 , C12N2795/10142 , C12N2795/18122 , C12N2795/18123 , C12P19/34 , C12Q1/6851 , C12Q1/702 , C12Q1/706 , C12Q1/707 , C12Q2600/158 , C12Q2545/113
摘要： The present invention relates to nuclease resistant nucleic acids in general and ribonuclease resistant RNAs in particular. Methods of making and using such nucleic acids are disclosed.

95. 发明申请

US20050118719A1 Nucleic acid delivery and expression 审中-公开
标题翻译：核酸递送和表达
公开(公告)号：US20050118719A1
公开(公告)日：2005-06-02
申请号：US10494880
申请日：2002-11-07
申请人： Michael Schmidt , David Schofield , Caroline Westwater , Joseph Dolan , Brian Hoel , Philip Werner , James Norris , Laura Kasman
发明人： Michael Schmidt , David Schofield , Caroline Westwater , Joseph Dolan , Brian Hoel , Philip Werner , James Norris , Laura Kasman
IPC分类号： C07K14/01 , C12N1/21 , C12N15/63 , C12N15/73 , C12N15/74 , C07H21/04
CPC分类号： C12N15/74 , C07K14/005 , C12N15/63 , C12N15/635 , C12N15/73 , C12N2795/10122
摘要： The invention provides methods and materials involved in delivering nucleic acid to cells and regulating expression of nucleic acid in cells.
摘要翻译：本发明提供了将核酸递送至细胞并调节核酸在细胞中的表达所涉及的方法和材料。

96. 发明申请

US20040039168A1 Method for manufacture of nanostructures using staged-assembly 审中-公开
标题翻译：使用分段组装制造纳米结构的方法
公开(公告)号：US20040039168A1
公开(公告)日：2004-02-26
申请号：US10371073
申请日：2003-02-21
发明人： Edward B. Goldberg
IPC分类号： C07K014/005 , C12P021/06
CPC分类号： C12N7/00 , B82Y5/00 , C12N2795/10122 , C12N2795/10131
摘要： Nanostructures formed from a plurality of structural units in which the positions of the structural units relative to each other are established in a defined geometry, are formed by sequentially adding structural units to a growing structure to build up the nanostructure. The nanostructure includes two or more species of protein structural units, and each structural unit is added to the growing structure in a separate structural unit-addition step. Structural units not incorporated in the growing structure is removed at the end of each structural unit-addition step. Each species of structural unit has the ability to assemble non-covalently with the growing nanostructure to which it is added but cannot self-assemble with other structural units of the same species.
摘要翻译：由多个结构单元形成的纳米结构通过依次将结构单元添加到生长结构以形成纳米结构而形成，其中结构单元相对于彼此的位置在确定的几何形状中建立。纳米结构包括两种或更多种蛋白质结构单元，并且在单独的结构单元添加步骤中将每个结构单元添加到生长结构中。不结合生长结构的结构单元在每个结构单元添加步骤结束时被去除。每种结构单元都具有与其添加的生长的纳米结构非共价组装的能力，但不能与相同物种的其它结构单元自组装。

97. 发明申请

US20030092134A1 Nucleic acid encoding 3'-5' exonuclease of bacteriophage RM 378 审中-公开
标题翻译：编码噬菌体RM378的5'-5'核酸外切酶的核酸
公开(公告)号：US20030092134A1
公开(公告)日：2003-05-15
申请号：US10270859
申请日：2002-10-11
申请人： Prokaria ltd.
发明人： Sigridur Hjorleifsdottir , Gudmundur O. Hreggvidsson , Olafur H. Fridjonsson , Arnthor Aevarsson , Jakob K. Kristjansson
IPC分类号： C12Q001/68 , C12P021/06 , C12P019/34 , A61K039/12
CPC分类号： C07K14/005 , C12N7/00 , C12N2795/10121 , C12N2795/10122 , Y10T436/143333
摘要： A novel bacteriophage RM 378 of Rhodothermus marinus, the nucleic acids of its genome, nucleic acids comprising nucleotide sequences of open reading frames (ORFs) of its genome, and polypeptides encoded by the nucleic acids, are described.
摘要翻译：描述了Rhodothermus marinus的新型噬菌体RM378，其基因组的核酸，其包含其基因组的开放阅读框（ORF）的核苷酸序列的核酸和由核酸编码的多肽。

98. 发明授权

US5871976A Autocatalytic replication of recombinant RNA 失效
标题翻译：重组RNA的自动催化复制
公开(公告)号：US5871976A
公开(公告)日：1999-02-16
申请号：US467816
申请日：1995-06-06
申请人： Fred Russell Kramer , Eleanor Anne Miele , Donald Robert Mills
发明人： Fred Russell Kramer , Eleanor Anne Miele , Donald Robert Mills
IPC分类号： C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： C12N7/00 , C12N15/10 , C12P19/34 , C12Q1/6867 , C12N2795/10122 , C12N2795/18122
摘要： This invention provides a method for producing a mutant virus or viroid, or a mutant recombinant RNA molecule comprising a single stranded virus genome or a single stranded viroid genome, a circular virus genome or a circular viroid genome, or a segment of a virus genome or a segment of a viroid genome which comprises incubating a recombinant single-stranded RNA molecule comprising a recognition sequence for the binding of an RNA directed RNA polymerase, a sequence for the initiation of product strand synthesis by the polymerase and a heterologous sequence of interest derived from a different RNA molecule inserted at a specific site in the internal region of the recombinant molecule under appropriate selective conditions and for a sufficient period of time permitting the selection of a mutant population, the heterologous sequence of interest comprising the RNA of the single stranded virus genome or the single stranded viroid genome, the circular virus genome or the circular viroid genome or a segment of the virus genome or a segment of the viroid genome.
摘要翻译：本发明提供了用于产生突变病毒或病毒的方法或包含单链病毒基因组或单链病毒基因组，环状病毒基因组或环状病毒基因组或病毒基因组的片段的突变型重组RNA分子或一种病毒基因组的片段，其包括孵育包含用于RNA定向RNA聚合酶结合的识别序列的重组单链RNA分子，通过聚合酶引发产物链合成的序列和衍生自在合适的选择性条件下插入重组分子的内部区域的特定位点的不同RNA分子，并且允许选择突变群体足够长的时间段，包含单链病毒基因组的RNA的异源感兴趣序列或单链类病毒基因组，环状病毒基因组或环状病毒基因组我或病毒基因组的一段或病毒基因组的片段。

99. 发明授权

US5864013A Materials for the production of nanometer structures and use thereof 失效
公开(公告)号：US5864013A
公开(公告)日：1999-01-26
申请号：US542003
申请日：1995-10-12
申请人： Edward B. Goldberg
发明人： Edward B. Goldberg
IPC分类号： C12N15/09 , B82B1/00 , C07H21/04 , C07K14/005 , C07K14/01 , C07K14/195 , C07K19/00 , C12N7/00 , C12N15/74 , C12P21/02 , C12R1/19 , C07K14/00 , C12P21/06
CPC分类号： C07K14/005 , C07K2319/00 , C07K2319/735 , C12N2795/10122 , Y10S977/70 , Y10S977/802 , Y10S977/803 , Y10S977/804 , Y10S977/84 , Y10S977/918 , Y10S977/92
摘要： The present invention pertains to nanostructures, i.e., nanometer sized structures useful in the construction of microscopic and macroscopic structures. In particular, the present invention pertains to nanostructures based on bacteriophage T4 tail fiber proteins and variants thereof.

100. 发明授权

US5602001A Cell-free method for synthesizing a protein 失效
标题翻译：无细胞合成蛋白的方法
公开(公告)号：US5602001A
公开(公告)日：1997-02-11
申请号：US235199
申请日：1994-04-29
申请人： Fred R. Kramer , Eleanor A. Miele , Donald R. Mills
发明人： Fred R. Kramer , Eleanor A. Miele , Donald R. Mills
IPC分类号： C12N15/10 , C12P19/34 , C12Q1/68 , C12P21/02 , C12N15/09
CPC分类号： C12N7/00 , C12N15/10 , C12P19/34 , C12Q1/6867 , C12N2795/10122 , C12N2795/18122
摘要： This invention concerns recombinant RNA molecules comprising a recognition sequence for the binding of an RNA-directed RNA polymerase, a sequence for the initiation of product strand synthesis-and a heterologous sequence of interest inserted at a specific site in the internal region of the recombinant molecule. Such recombinant RNA molecules are capable of serving as a template for the synthesis of complementary single-stranded molecules by RNA-directed RNA polymerase. The product molecules so formed are also capable of serving as a template for the synthesis of additional copies of the original recombinant RNA molecule. In a preferred embodiment of the invention Q.beta. replicase is used as the RNA-directed RNA polymerase. The invention also concerns methods of selectively cleaving RNA molecules at specific sites, methods of constructing recombinant RNA molecules, and methods for the autocatalytic in vitro replication of recombinant RNA molecules. The recombinant RNA molecules of this invention, or fragments thereof, are useful in methods of sequencing nucleic acids, as hybridization probes, as labels, in cell-free methods of protein synthesis, in methods for identifying or characterizing RNA processing enzymes, in methods for producing mutant viruses or viroids and in isolating from mRNA mixtures a desired mRNA in the form of a recombinant mRNA molecule.
摘要翻译：本发明涉及重组RNA分子，其包含用于结合RNA指导的RNA聚合酶的识别序列，用于引发产物链合成的序列和在重组分子的内部区域中的特定位点插入的异源感兴趣的序列。这样的重组RNA分子能够作为通过RNA指导的RNA聚合酶合成互补单链分子的模板。如此形成的产物分子也能够用作合成原始重组RNA分子的额外拷贝的模板。在本发明的优选实施方案中，使用Qβ复制酶作为RNA指导的RNA聚合酶。本发明还涉及在特定部位选择性切割RNA分子的方法，构建重组RNA分子的方法以及重组RNA分子的自动催化体外复制的方法。本发明的重组RNA分子或其片段可用于测序核酸的方法，作为杂交探针，作为标记，在无细胞蛋白质合成方法中，用于鉴定或表征RNA加工酶的方法中，在产生突变病毒或类病毒，并从mRNA混合物中分离出重组mRNA分子形式的所需mRNA。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式