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91. 发明申请

US20120122201A1 Gas Flow Rate And Liquid Level Monitoring Apparatus 审中-公开
标题翻译：气体流量和液位监测仪
公开(公告)号：US20120122201A1
公开(公告)日：2012-05-17
申请号：US13384767
申请日：2010-07-12
申请人： Stephen James Butler , Stephen Mark Joseph Wilkins , David Wingate
发明人： Stephen James Butler , Stephen Mark Joseph Wilkins , David Wingate
IPC分类号： C12M1/00 , G01F13/00
CPC分类号： C12M23/22 , C12M41/00 , C12M41/12 , C12M41/14 , C12M41/34 , C12M41/44 , G01F23/00 , G01F23/02
摘要： An incubator (10) comprising an incubator housing (14), at least one sealable incubation chamber (16) in the housing (14), means for controlling temperature, humidity and gas composition within the incubation chamber (16), a liquid container (26), and a window (24) for observing the gas flow rate and liquid level of said a liquid container (26), said container having at least one light transmissible side wall (38), at least one gas inlet conduit (28) having a gas outlet at or adjacent to the said at least one light transmissible side wall (38), a gas outlet conduit (32) having a gas inlet which is spaced above the gas outlet of the said at least one gas inlet conduit (28), and a light emitting device (34) for directing a beam of light to be incident with or adjacent to an interior surface (46) of the said at least one light transmissible side wall (38) so as to in use illuminate at least one gas bubble (44) discharged from the gas outlet.
摘要翻译：一种培养箱（10），包括培养箱壳体（14），所述壳体（14）中的至少一个可密封的孵育室（16），用于控制所述孵育室（16）内的温度，湿度和气体组成的装置，液体容器 26），以及用于观察所述液体容器（26）的气体流量和液面的窗口（24），所述容器具有至少一个透光侧壁（38），至少一个气体入口导管（28）在所述至少一个透光侧壁（38）处或邻近所述至少一个透光侧壁（38）处具有气体出口，气体出口导管（32），其具有在所述至少一个气体入口导管（28）的气体出口上方间隔开的气体入口）和用于引导与所述至少一个透光侧壁（38）的内表面（46）入口或邻近所述至少一个透光侧壁（38）的光束的发光装置（34），以便在使用中照亮至少从气体出口排出一个气泡（44）。

92. 发明申请

US20120083030A1 INCUBATOR 有权
公开(公告)号：US20120083030A1
公开(公告)日：2012-04-05
申请号：US13241619
申请日：2011-09-23
申请人： Hiroki Busujima , Yoshihiro Takahashi
发明人： Hiroki Busujima , Yoshihiro Takahashi
IPC分类号： C12M1/00
CPC分类号： C12M41/14 , C12M37/00
摘要： An incubator comprising: a culture chamber configured to accommodate culture; a dish structure configured to contain a liquid; an ultrasonic vibrator provided in a part of the dish structure, the ultrasonic vibrator configured to atomize the liquid; and a gas-liquid contact structure configured to bring the atomized liquid into contact with air in the chamber to be cultured.
摘要翻译：一种培养箱，包括：构造成适应培养的培养室; 配置为容纳液体的盘结构; 超声波振动器，设置在所述碟形结构的一部分中，所述超声波振动器被配置为使所述液体雾化; 以及气液接触结构，其构造成使所述雾化液体与待培养的所述室内的空气接触。

93. 发明申请

US20110171724A1 Apparatus and Method for Removing Microbial Contaminants From a Flowing Fluid 审中-公开
标题翻译：从流动流体中去除微生物污染物的装置和方法
公开(公告)号：US20110171724A1
公开(公告)日：2011-07-14
申请号：US12620519
申请日：2009-11-17
申请人： Dan M. Sheldon
发明人： Dan M. Sheldon
IPC分类号： C12M1/12
CPC分类号： C12M37/02 , C12M41/14
摘要： Methods and apparatuses for removing microbial contaminants from a flowing fluid in a cell culture incubator are disclosed. Some embodiments of the invention provide a cell culture incubator including a chamber, an airflow passage through which gasses circulate within the chamber, a filter configured to filter gasses that flow through the airflow passage and chamber, and a blower for circulating gasses through the airflow passage, chamber and filter. The blower includes a structural component at least partially formed from an anti-microbial material.
摘要翻译：公开了从细胞培养培养箱中流动的流体中除去微生物污染物的方法和装置。本发明的一些实施方案提供了一种细胞培养培养箱，其包括室，气体在气室内循环的气流通道，被配置为过滤流过气流通道和室的气体的过滤器和用于使气体循环通过气流通道的鼓风机，室和过滤器。鼓风机包括至少部分地由抗微生物材料形成的结构部件。

94. 发明授权

US07968335B2 Cell culturing method using biomechanical stimulation loading and system therefor 有权
标题翻译：使用生物力学刺激装载的细胞培养方法及其系统
公开(公告)号：US07968335B2
公开(公告)日：2011-06-28
申请号：US11629619
申请日：2005-06-16
申请人： Ken Nakata
发明人： Ken Nakata
IPC分类号： C12N5/00
CPC分类号： C12M35/04 , C12M23/50 , C12M41/14
摘要： A cell culturing method employing biomechanical stimulation loading and a system for applying, in in vitro culture, stimulation similar to vertical weight load stimulation or sideward shear stress stimulation acting on tissues in an aboveground living body as repeated biomechanical stimulation to culture cells, culture tissues or a construct including the culture cells. The system includes a mechanical stimulation loading device and a culture receptacle that are placed in a carbon dioxide incubator, and a control computer. The device includes a weight loading piston, a piston raising and lowering stage supporting piston to allow vertical movement of a piston within a designated range, and a stage lifting and lowering mechanism for vertically moving the stage. A computer drives the mechanism in a specified cycle to effect the vertical movement of the stage. When the stage is lowered, a culture in the receptacle is weighted with the piston.
摘要翻译：使用生物力学刺激加载的细胞培养方法和用于在体外培养中应用类似于在地上生物体中的组织上作用的垂直重量负荷刺激或侧向剪切应力刺激的刺激的系统，作为对培养细胞，培养组织或重复的生物力学刺激的系统包括培养细胞的构建体。该系统包括放置在二氧化碳培养箱中的机械刺激加载装置和培养容器以及控制计算机。该装置包括重量装载活塞，活塞升降台支撑活塞以允许活塞在指定范围内的垂直运动，以及用于垂直移动平台的台升降机构。计算机在指定的周期内驱动机构以实现平台的垂直运动。当舞台下降时，容器中的培养物用活塞加重。

95. 发明申请

US20110070631A1 Container transportation case and culture treating device 审中-公开
标题翻译：集装箱运输箱和文化处理装置
公开(公告)号：US20110070631A1
公开(公告)日：2011-03-24
申请号：US12923388
申请日：2010-09-17
申请人： Yasujiro Kiyota
发明人： Yasujiro Kiyota
IPC分类号： C12M3/00
CPC分类号： C12M23/54 , C12M37/00 , C12M41/14
摘要： A container transportation case is used to carry in/out a culture container to/from a chamber of a culture treating device. A case body of the container transportation case has a container housing room in which the culture container can be arranged and the container housing room is in communication with the outside by an opening part. A joint unit of the container transportation case can be attached/removed to/from a carrying-in/out opening of the culture treating device, and forms a space that is isolated from an outside atmosphere by joining the container housing room and the carrying-in/out opening. An airtight member of the container transportation case closes the opening part to block the container housing room from the outside atmosphere.
摘要翻译：容器运输箱用于将培养容器携带到培养处理装置的室中或从培养处理装置的室中进出。容器运输箱的箱体具有容纳容纳室，培养容器可以布置在容器容纳室中，并且容器容纳室通过开口部与外部连通。容器运输箱的联合单元可以连接/移出文化处理装置的进/出开口，并且通过将容器容纳室和携带物体接合而形成与外部气氛隔离的空间，进/出口。集装箱运输箱的密封件关闭开口部分，以从外部大气阻挡容器容纳室。

96. 发明申请

US20110002525A1 Method for distinguishing living cells during cell observation, image processing program for cell observation, and image processing device 有权
标题翻译：细胞观察期间区分活细胞的方法，细胞观察用图像处理程序和图像处理装置
公开(公告)号：US20110002525A1
公开(公告)日：2011-01-06
申请号：US12923144
申请日：2010-09-03
申请人： Masafumi Mimura , Kei Ito
发明人： Masafumi Mimura , Kei Ito
IPC分类号： G06K9/00
CPC分类号： G06K9/0014 , C12M23/48 , C12M41/14 , C12M41/36 , C12M41/46 , G06T7/0012 , G06T7/254 , G06T2207/10056 , G06T2207/30024
摘要： An image processing program for cell observation in which living cells can be distinguished from foreign matter other than living cells during cell observation under wide observation conditions. The image processing program includes a step for capturing first and second images of an object positioned in the observation field of view at a predetermined time interval, a step for extracting a representation of the object shown in the obtained images, steps for aligning the rotational angular orientation of the object in the image plane and computing a correlation value, a step for determining whether the object is a living cell on the basis of the computed correlation value, and steps for outputting the result of the decision made with regard to the object.
摘要翻译：一种用于细胞观察的图像处理程序，其中在广泛的观察条件下，细胞观察期间活细胞可以与活细胞以外的异物区分开。图像处理程序包括以预定时间间隔捕获位于观察视野中的物体的第一和第二图像的步骤，用于提取所获得图像中所示的对象的表示的步骤，用于对准旋转角并且计算相关值，基于计算的相关值来确定对象是活细胞的步骤，以及用于输出关于对象的决定结果的步骤。

97. 发明申请

US20100328434A1 Microscope apparatus and cell culture apparatus 有权
标题翻译：显微镜装置和细胞培养装置
公开(公告)号：US20100328434A1
公开(公告)日：2010-12-30
申请号：US12923004
申请日：2010-08-27
申请人： Yasujiro Kiyota
发明人： Yasujiro Kiyota
IPC分类号： H04N13/02 , G02B21/36
CPC分类号： C12M41/14 , C12M41/46 , G01N1/312 , G01N35/0099 , G02B21/241 , G02B21/26 , G02B21/367
摘要： An imaging section of a microscope apparatus captures a plurality of microscope images each having the focal position which differs in the field being same with a light flux having passed through a microscopic optical system. A region separating section separates a cellular region from a non-cellular region by using the plurality of the microscope images. A focusing position calculating section finds a focusing position in a target pixel included in the cellular region based on a brightness change in the position being same in the plurality of the microscope images. A three dimensional information generating section generates three dimensional information of a cultured cell based on a position of the cellular region and the focusing position in the target pixel.
摘要翻译：显微镜装置的成像部分捕获多个显微镜图像，每个显微镜图像具有与通过微观光学系统的光通量相同的场中的焦点位置。区域分离部分通过使用多个显微镜图像将细胞区域与非细胞区域分离。聚焦位置计算部基于多个显微镜图像中的相同位置的亮度变化来求出包含在细胞区域中的目标像素中的聚焦位置。三维信息生成部基于目标像素中的细胞区域的位置和聚焦位置，生成培养细胞的三维信息。

98. 发明授权

US07838286B2 Incubator 有权
标题翻译：孵化器
公开(公告)号：US07838286B2
公开(公告)日：2010-11-23
申请号：US10947553
申请日：2004-09-23
申请人： Yuichi Tamaoki , Yasushi Sakata , Hidetoshi Shinya , Hiroki Busujima
发明人： Yuichi Tamaoki , Yasushi Sakata , Hidetoshi Shinya , Hiroki Busujima
IPC分类号： C12M1/00 , C12M3/00
CPC分类号： C12M41/14
摘要： An entry of air from the outside of an incubator into an incubation room is prevented. The incubator comprises an adiabatic box main body having an opening on a front face thereof, an adiabatic door mounted to the adiabatic box main body to be opened/closed, a transparent inner door which closes the opening to be opened, and a storeroom surrounded with the inner door and the adiabatic box main body to incubate samples such as cells or microbes. The adiabatic box main body comprises a metal outer box, a metal inner box, a heat insulating material arranged in the outer box between the outer box and the inner box, and an air layer arranged more inside than the heat insulating material. Pressure of the storeroom is set higher than that of an outside space of the incubator.
摘要翻译：防止空气从孵化器外进入孵化室。培养箱包括：绝热箱主体，其前表面具有开口;绝热门，其安装在绝热箱主体上以打开/关闭;封闭打开的开口的透明内门;以及包围着的储藏室内门和绝热盒主体，以孵育细胞或微生物样品。绝热箱主体包括金属外箱，金属内箱，布置在外箱和内箱之间的外箱中的隔热材料以及比隔热材料更内侧布置的空气层。储藏室的压力高于孵化器的外部空间。

99. 发明申请

US20100167383A1 CULTURE APPARATUS 有权
标题翻译：文化装置
公开(公告)号：US20100167383A1
公开(公告)日：2010-07-01
申请号：US12703532
申请日：2010-02-10
申请人： Hiroki Busujima , Yasuhiro Kikuchi , Kenichi Ito , Kuniyoshi Kobayashi
发明人： Hiroki Busujima , Yasuhiro Kikuchi , Kenichi Ito , Kuniyoshi Kobayashi
IPC分类号： C12M1/36
CPC分类号： C12M41/14 , A61L2/10 , C12M37/00 , C12M37/06 , Y10S435/809
摘要： A culture apparatus comprising: a housing including a culture chamber inside, culture being cultured in the culture chamber; a door configured to seal the culture chamber; an ultraviolet lamp configured to generate an ultraviolet ray for disinfecting air in the culture chamber; a lighting device configured to allow the ultraviolet lamp to be on for a predetermined time period, when the air in the culture chamber is disinfected; a detecting device configured to detect deterioration of disinfection capability of the ultraviolet lamp; and a controller configured to correct the predetermined time period, during which the ultraviolet lamp is on, so as to be longer according to the deterioration of the disinfection capability of the ultraviolet lamp detected by the detecting device.
摘要翻译：一种培养装置，其特征在于，包括：在培养室内培养的培养室内的培养室，构造成密封培养室的门; 紫外灯，其构造成产生用于对培养室中的空气进行消毒的紫外线; 照明装置，被配置为当所述培养室中的空气被消毒时允许所述紫外线灯处于预定时间段; 检测装置，其被配置为检测所述紫外线灯的消毒能力的劣化; 以及控制器，其被配置为根据由所述检测装置检测到的所述紫外线灯的消毒能力的劣化来校正所述紫外线灯在其中导通的所述预定时间段。

100. 发明授权

US07678568B2 Incubator 有权
标题翻译：孵化器
公开(公告)号：US07678568B2
公开(公告)日：2010-03-16
申请号：US11330201
申请日：2006-01-12
申请人： Hiroshi Yamamoto , Yasuhiko Yokoi , Mikio Houjou , Daisuke Etou , Ayako Michida , Akihiko Yamada , Akira Sakaguchi , Masaki Harada , Hiroki Busujima , Yuichi Tamaoki
发明人： Hiroshi Yamamoto , Yasuhiko Yokoi , Mikio Houjou , Daisuke Etou , Ayako Michida , Akihiko Yamada , Akira Sakaguchi , Masaki Harada , Hiroki Busujima , Yuichi Tamaoki
IPC分类号： C12M1/00 , C12M3/00
CPC分类号： C12M41/14 , C12M23/48 , C12M23/50 , C12M41/36 , C12M41/48 , Y10S435/809
摘要： The invention provides an incubator 1 wherein stackers 3 having a plurality of microplate accommodating portions are arranged in a chamber 11, and a microplate transport device 5 is arranged for transporting a microplate 31 within the chamber 11 and moving the microplate 31 into or out of a desired microplate accommodating portion. A camera 7 is provided on a position opposed to a microplate accommodating portion of an uppermost stage in the stacker 3. The camera 7 faces said microplate accommodating portion, whereby a sample on the microplate 31 can be photographed.
摘要翻译：本发明提供了一种培养箱1，其中具有多个微孔板容纳部分的堆积器3布置在室11中，并且微孔板传送装置5布置成用于运送室11内的微板31并将微孔板31移入或移出所需的微孔板容纳部分。相机7设置在与堆叠器3中的最上层的微孔板容纳部分相对的位置上。相机7面对所述微孔板容纳部分，从而可以拍摄微孔板31上的样品。

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