专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

91. 发明申请

US20110266225A1 CHROMATOGRAPHY LIGAND 有权
标题翻译：色谱仪
公开(公告)号：US20110266225A1
公开(公告)日：2011-11-03
申请号：US13158780
申请日：2011-06-13
申请人： BO-LENNART JOHANSSON , JEAN-LUC MALOISEL , HANS J. JOHANSSON , ASA EK , MARIA HOLM , JAFAR SAIDZADEH
发明人： BO-LENNART JOHANSSON , JEAN-LUC MALOISEL , HANS J. JOHANSSON , ASA EK , MARIA HOLM , JAFAR SAIDZADEH
IPC分类号： B01D15/08 , B23P17/04
CPC分类号： B01D15/327 , B01D15/363 , B01D15/3804 , B01J20/28033 , B01J20/286 , B01J20/3219 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/16 , C07K1/18 , C07K1/36 , C07K16/00 , C07K16/065 , Y10T29/49826
摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
摘要翻译：本发明涉及由下式R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。

92. 发明申请

US20110177601A1 METHOD FOR CONCENTRATION OF LOW-MOLECULAR-WEIGHT PROTEINS AND PEPTIDES IN BODY FLUID SAMPLE 有权
标题翻译：浓度低分子量蛋白质和体内液体样品的方法
公开(公告)号：US20110177601A1
公开(公告)日：2011-07-21
申请号：US12672677
申请日：2008-08-08
申请人： Yoshio Kodera , Tadakazu Maeda , Yusuke Kawashima
发明人： Yoshio Kodera , Tadakazu Maeda , Yusuke Kawashima
IPC分类号： G01N1/28
CPC分类号： G01N1/34 , C07K1/145 , C07K1/30 , C07K1/36 , G01N1/4044 , Y10T436/107497 , Y10T436/25 , Y10T436/25375 , Y10T436/255
摘要： A method for extracting low-molecular-weight proteins/peptides contained in a body fluid sample, particularly, in serum or plasma. The method includes the steps of (a) to (e): (a) adding reagent 1 containing urea and thiourea and reagent 2 containing a reducing agent to the body fluid sample, mixing them, subsequently dropping the mixture into reagent 3 containing 90% or more of an organic solvent, and mixing them; (b) stirring at a low temperature the mixed solution obtained in step (a); (c) centrifuging at a low temperature the stirred solution obtained in step (b) and removing the supernatant; (d) adding reagent 4 containing an organic solvent and an acid to the precipitate obtained in step (c) and mixing them; (e) stirring at a low temperature the mixed solution obtained in step (d); and (f) centrifuging at a low temperature the stirred solution obtained in step (e) and recovering the supernatant.
摘要翻译：用于提取体液样品中特别是血清或血浆中所含的低分子量蛋白质/肽的方法。该方法包括以下步骤：（a）将含有尿素和硫脲的试剂1和含有还原剂的试剂2加入到体液样品中，混合，然后将混合物倒入含有90％或更多的有机溶剂，并将它们混合; （b）在低温下搅拌步骤（a）中得到的混合溶液; （c）在低温下离心步骤（b）中获得的搅拌溶液并除去上清液; （d）向步骤（c）中得到的沉淀物中加入含有有机溶剂和酸的试剂4并混合; （e）在低温下搅拌步骤（d）中得到的混合溶液; 和（f）在低温下离心步骤（e）中获得的搅拌溶液并回收上清液。

93. 发明授权

US07795010B2 Fractionator and method of fractionation 有权
$Fractionator and method of fractionation$
标题翻译：分馏器和分馏方法
公开(公告)号：US07795010B2
公开(公告)日：2010-09-14
申请号：US10586678
申请日：2005-01-20
申请人： Kazuhiro Tanahashi , Ichiro Kumo , Nobuyuki Kuroki , Hiroyuki Sugaya , Satoko Yamada , Shigehisa Wada , Giman Jung , Toshihiko Kuroda , Shuji Sekiguchi
发明人： Kazuhiro Tanahashi , Ichiro Kumo , Nobuyuki Kuroki , Hiroyuki Sugaya , Satoko Yamada , Shigehisa Wada , Giman Jung , Toshihiko Kuroda , Shuji Sekiguchi
IPC分类号： C12M3/00
CPC分类号： B01D63/04 , B01D61/142 , B01D61/20 , B01D63/08 , B01D2313/243 , B01D2319/022 , C07K1/22 , C07K1/34 , C07K1/36
摘要： The following is disclosed: (1) a membrane fractionator including a filtration section, a concentrating section, a recovery section and a liquid feed pump, wherein a flow channel connecting the filtration section, concentrating section and recovery section to each other constitutes a closed circuitry; (2) a method of biocomponent separation, characterized in that a sample derived from biocomponents is fed into an antibody adsorption membrane separation system having an antibody capable of adsorbing specified protein internally accommodated in the middle or a rear part of a membrane separation system that in the absence of antibodies adsorbing proteins, exhibits a permeation ratio between human alpha1-microglobulin and human albumin of 1.5 to 1000, thereby separating part of the biocomponents; and (3) a method of protein fractionation, comprising bringing a solution containing two or more types of proteins and water into contact with a hollow yarn separation membrane to thereby attain protein fractionation, characterized in that the fractionation solution contains an organic solvent.
摘要翻译：以下公开了：（1）包括过滤部分，浓缩部分，回收部分和液体供给泵的膜分馏器，其中将过滤部分，浓缩部分和回收部分彼此连接的流动通道构成闭合电路 ; （2）一种生物成分分离方法，其特征在于，将来自生物成分的样品加入抗体吸附膜分离系统中，所述抗体吸附膜分离系统具有能够吸附在膜分离系统的中部或后部内部容纳的特定蛋白质的抗体，不存在吸附蛋白质的抗体，人α1-微球蛋白与人白蛋白之间的渗透比为1.5〜1000，从而分离部分生物成分; （3）蛋白质分级法的方法，其特征在于，使含有2种以上蛋白质和水的溶液与中空丝分离膜接触，从而进行蛋白质分馏，其特征在于，分离液含有有机溶剂。

94. 发明申请

US20100228011A1 BENIGN SOLVENTS FOR FORMING PROTEIN STRUCTURES 有权
标题翻译：用于形成蛋白质结构的固体溶剂
公开(公告)号：US20100228011A1
公开(公告)日：2010-09-09
申请号：US12571043
申请日：2009-09-30
申请人： Bin Dong , Olivier Arnoult , Gary Wnek , Lingui Meng
发明人： Bin Dong , Olivier Arnoult , Gary Wnek , Lingui Meng
IPC分类号： C07K1/14 , B01F1/00
CPC分类号： C07K1/36 , C07K1/145
摘要： A benign solvent for dissolving proteins comprises alcohol, salt and water. The ratio by volume of water to alcohol is between about ninety-nine-to-one and about one-to-ninety-nine. A salt concentration is between near zero moles per liter and the maximum salt concentration soluble in water. The amount of protein by weight as compared to the mixture of water and alcohol is between near zero percent and about 25 percent. A method for forming a protein structure from a benign solvent comprises forming a benign solvent from water, alcohol, and salt; and dissolving a protein in the benign solvent to form a protein solution. The method further comprises extracting the protein from the protein solution; and arranging the protein into a protein structure.
摘要翻译：用于溶解蛋白质的良性溶剂包括酒精，盐和水。水与酒精的体积比为约九十九比一至约九十九分之一。盐浓度在接近零摩尔/升和最大盐浓度溶于水。与水和醇的混合物相比，重量比的蛋白质的量在接近零％和约25％之间。从良性溶剂形成蛋白质结构的方法包括从水，醇和盐形成良溶剂; 并将蛋白质溶解在良溶剂中以形成蛋白质溶液。该方法还包括从蛋白质溶液中提取蛋白质; 并将蛋白质排列成蛋白质结构。

95. 发明申请

US20100228006A1 Process for purifying lipopeptides 有权
标题翻译：脂肽纯化方法
公开(公告)号：US20100228006A1
公开(公告)日：2010-09-09
申请号：US12708539
申请日：2010-02-19
申请人： Martin Mansson , Eli Karin Dale , Sissel Hauge , Carsten Overballe-Petersen , Kjersti Aastorp Hirth , Dennis Brian Hansen
发明人： Martin Mansson , Eli Karin Dale , Sissel Hauge , Carsten Overballe-Petersen , Kjersti Aastorp Hirth , Dennis Brian Hansen
IPC分类号： C07K1/36
CPC分类号： C12P17/188 , C07K1/36 , C12P21/02
摘要： The present invention relates to a process for purifying lipopeptides. More particular, the invention provides an improved method for purifying daptomycin.
摘要翻译：本发明涉及一种纯化脂肽的方法。更具体地，本发明提供了一种用于纯化达托霉素的改进方法。

96. 发明授权

US07759117B2 Use of depth filtration in series with continuous centrifugation to clarify mammalian cell cultures 有权
标题翻译：使用深度过滤串联连续离心澄清哺乳动物细胞培养
公开(公告)号：US07759117B2
公开(公告)日：2010-07-20
申请号：US11616772
申请日：2006-12-27
申请人： Christine Y. Pham
发明人： Christine Y. Pham
IPC分类号： C12S3/14
CPC分类号： C12N1/02 , C07K1/30 , C07K1/34 , C07K1/36 , C12M47/02 , Y10S435/803
摘要： Methods for clarification of cell samples using centrifugation in combination with depth filtration.
摘要翻译：使用离心与深度过滤相结合的细胞样品的澄清方法。

97. 发明申请

US20100041611A1 Rapid Isolation of Osteoinductive Protein Mixtures From Mammalian Bone Tissue 有权
标题翻译：从哺乳动物骨组织快速分离骨诱导蛋白混合物
公开(公告)号：US20100041611A1
公开(公告)日：2010-02-18
申请号：US12582476
申请日：2009-10-20
申请人： Kevin Thorne , Rama AKELLA
发明人： Kevin Thorne , Rama AKELLA
IPC分类号： A61K38/16 , A61P19/00
CPC分类号： C07K1/34 , A61K35/32 , A61K38/1875 , C07K1/36 , C07K14/51 , Y10S530/84
摘要： A method for purifying bone-derived osteoinductive proteins including a demineralization process, a protein extraction process, a high molecular weight ultrafiltration process, a low molecular weight ultrafiltration process, and a recover process. The high and low ultrafiltration processes preferably select proteins having a nominal molecular weight between approximately 8 kilodaltons and approximately 100 kilodaltons. Processes of the present invention may be used to recover osteoinductive proteins from bone demineralization waste streams.
摘要翻译：一种用于纯化骨衍生的骨诱导蛋白的方法，包括脱矿质过程，蛋白质提取法，高分子量超滤法，低分子量超滤法和恢复法。高和低超滤方法优选选择标称分子量在约8千道尔顿和约100千道尔顿之间的蛋白质。本发明的方法可用于从骨脱矿质废物流中回收骨诱导蛋白。

98. 发明申请

US20080306249A1 METHOD OF SELECTIVELY REMOVING PROTEIN FROM BIOLOGICAL SAMPLES USING CHEMICALS 审中-公开
标题翻译：使用化学品从生物样品中选择性去除蛋白质的方法
公开(公告)号：US20080306249A1
公开(公告)日：2008-12-11
申请号：US12107498
申请日：2008-04-22
申请人： Myo-yong LEE , Ki-woong HAN
发明人： Myo-yong LEE , Ki-woong HAN
IPC分类号： C07K1/00
CPC分类号： C07K1/36 , C07K1/20 , C12Q1/6806 , C12Q2527/125
摘要： Provided is a method of removing protein while not removing nucleic acids from a biological sample containing protein, the method including: adding a compound of formula I below and a protein nucleating agent to the biological sample containing protein: where at least two of R1, R2, and R3 substituents are substituted or unsubstituted C1-C6 alkyl groups and the other substituent is a hydrogen atom or a substituted or unsubstituted C1-C6 alkyl group, a is an integer of 1 to 6 and b is 0 or 1, wherein b is 0 when a is not 1; treating the resultant mixture with a hydrophobic surface material in order to obtain a protein-free mixture; and separating the protein-free mixture from the hydrophobic surface material to which the protein is bound. By using the method, the protein can be selectively, effectively removed from the biological sample containing the protein while a nucleic acid is maintained in the sample.
摘要翻译：提供一种除去蛋白质而不从含有生物样品的蛋白质中除去核酸的方法，该方法包括：将下面的式I化合物和蛋白质成核剂加入到含有蛋白质的生物样品中：其中R1，R2中的至少两个和R3取代基是取代或未取代的C1-C6烷基，另一个取代基是氢原子或取代或未取代的C1-C6烷基，a是1〜6的整数，b是0或1，其中b是当a不为1时为0; 用疏水性表面材料处理所得混合物以获得无蛋白质的混合物; 并将蛋白质与蛋白质结合的疏水表面材料分离出来。通过使用该方法，可以在将样品中保持核酸的同时，从含有蛋白质的生物样品中有效地除去蛋白质。

99. 发明授权

US07439337B2 Three-phase partitioning method for purifying a protein 失效
标题翻译：用于纯化蛋白质的三相分配方法
公开(公告)号：US07439337B2
公开(公告)日：2008-10-21
申请号：US11832830
申请日：2007-08-02
申请人： William W. Ward
发明人： William W. Ward
IPC分类号： A23J1/00
CPC分类号： C07K1/14 , C07K1/36
摘要： The present invention is a kit and method for purifying a protein of interest.
摘要翻译：本发明是用于纯化目的蛋白质的试剂盒和方法。

100. 发明申请

US20080221310A1 Extraction of Cellular Components with Fatty Acid Derivatives 有权
标题翻译：用脂肪酸衍生物提取细胞成分
公开(公告)号：US20080221310A1
公开(公告)日：2008-09-11
申请号：US11915773
申请日：2006-05-30
申请人： Valerie J. O'Sullivan , Edward J. Conklin
发明人： Valerie J. O'Sullivan , Edward J. Conklin
IPC分类号： C07K1/14
CPC分类号： C07K1/30 , C07H13/06 , C07K1/36
摘要： A method of treating biologic material with a composition containing at least one fatty acid derivative and/or at least one fatty alcohol, a buffer, and a salt to result in protein partitioning into an aqueous phase and an organic phase. The protein may be a membrane protein. The cells may be plant cells and/or animal cells. The proteins can be thereafter be used for downstream applications.
摘要翻译：一种用含有至少一种脂肪酸衍生物和/或至少一种脂肪醇，缓冲剂和盐的组合物处理生物材料以产生蛋白质分配成水相和有机相的方法。蛋白质可以是膜蛋白质。细胞可以是植物细胞和/或动物细胞。此后可以将蛋白质用于下游应用。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式