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    • 91. 发明授权
    • Method for increasing seed yield or biomass by expressing RNA binding proteins in transgenic plants
    • 通过在转基因植物中表达RNA结合蛋白来提高种子产量或生物量的方法
    • US08455719B2
    • 2013-06-04
    • US11660395
    • 2005-08-16
    • Valerie FrankardChristophe ReuzeauAna Isabel Sanz Molinero
    • Valerie FrankardChristophe ReuzeauAna Isabel Sanz Molinero
    • C12N15/82C07H21/04A01H5/00
    • C12N15/8262C07K14/415C12N15/8261Y02A40/146
    • The invention concerns a method for improving growth characteristics of plants by increasing activity in a plant of an RNA-binding protein, which is: (i) a polypeptide having RNA-binding activity and comprising 2 or 3 RNA recognition motifs (RRMs) and a motif having at least 75% identity to motif I: PYEAAVVALPVVVKERLVRILRLGIATRYD (SEQ ID NO: 12) and/or a motif having at least 50% identity to motif II: RFDPFTGEPYKFDP (SEQ ID NO: 13); or (ii) an RBP1 polypeptide having (a) RNA-binding activity; (b) two RRM domains, (c) the following two motifs: (i) KIFVGGL (SEQ ID NO: 41); and (ii) RPRGFGF (SEQ ID NO: 42), allowing for up to three amino acid substitutions and any conservative change in the motifs; and (d) having at least 20% identity to SEQ ID NO: 15. Also provided is transgenic plants introduced with an RNA-binding protein-encoding nucleic acid having improved growth characteristics and constructs useful in the methods.
    • 本发明涉及通过增加植物中的RNA结合蛋白的活性来提高植物的生长特性的方法,其是:(i)具有RNA结合活性并包含2或3个RNA识别基序(RRM)的多肽和 基序与基序I具有至少75%的同一性:PYEAAVVALPVVKERLVRILRLGIATRYD(SEQ ID NO:12)和/或与基序II具有至少50%同一性的基序:RFDPFTGEPYKFDP(SEQ ID NO:13); 或(ii)具有(a)RNA结合活性的RBP1多肽; (b)两个RRM结构域,(c)以下两个基序:(i)KIFVGGL(SEQ ID NO:41); 和(ii)RPRGFGF(SEQ ID NO:42),允许多达3个氨基酸取代和图案中任何保守的变化; 和(d)与SEQ ID NO:15具有至少20%的同一性。还提供了引入具有改善的生长特性的RNA结合蛋白质编码核酸的转基因植物和在该方法中有用的构建体。