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    • 94. 发明授权
    • Probe and primer for tubercle bacillus detection, and method of detecting human tubercle bacillus therewith
    • 用于结核杆菌检测的探针和底物,以及用于检测人类结核杆菌的方法
    • US08628926B2
    • 2014-01-14
    • US13281421
    • 2011-10-25
    • Tomokazu Ishikawa
    • Tomokazu Ishikawa
    • C12Q1/68C07H21/04
    • C12Q1/689C12Q2600/16Y10S435/975
    • Primers and probes are provided for selectively detecting tubercle bacillus, and methods for detecting low copy numbers of human tubercle bacillus (Mycobacterium tuberculosis) using the same are presented. Oligonucleotides comprising a part of or an entire sequence of the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:S, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8, or a part of or an entire sequence of the complementary sequence thereof are disclosed, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis. Primers and probes containing such oligonucleotides, and methods for detecting Mycobacterium tuberculosis using the primers and the probes are presented.
    • 提供了引物和探针用于选择性检测结核杆菌,并提出了使用该结核杆菌检测人结核杆菌(Mycobacterium tuberculosis)的低拷贝数的方法。 包含SEQ ID NO:1,SEQ ID NO:2,SEQ ID NO:3,SEQ ID NO:4,SEQ ID NO:S,SEQ ID NO:3所示的核酸序列的一部分或全部序列的寡核苷酸 6,SEQ ID NO:7或SEQ ID NO:8,或其互补序列的一部分或全部序列,其中寡核苷酸具有与结核分枝杆菌中IS6110基因的核酸序列杂交的性质。 提供了含有此类寡核苷酸的引物和探针,以及使用引物和探针检测结核分枝杆菌的方法。
    • 99. 发明申请
    • Primer and Probe for Use In Detection of Mycobacterium Kansasii and Method for Detection of Mycobacterium Kansasii Using The Same
    • 用于检测堪萨斯分枝杆菌的引物和探针及其使用的堪萨斯分枝杆菌检测方法
    • US20130065231A1
    • 2013-03-14
    • US13572392
    • 2012-08-10
    • Tomokazu Ishikawa
    • Tomokazu Ishikawa
    • C12Q1/68G01N21/64G01N21/76
    • C12Q1/04C12Q1/689G01N33/56922G01N2333/35
    • The present invention discloses an oligonucleotide which comprises a part or the entire sequence of the nucleotide sequence depicted in SEQ ID NOS: 1-4, or a part or the entire sequence of a nucleotide sequence complementary to SEQ ID NOS: 1-4, wherein the oligonucleotide is capable of hybridizing with the nucleotide sequence of Mycobacterium kansasii; a primer and a probe for detecting M. kansasii comprising the oligonucleotide; and a method for detecting M. kansasii using the primer and/or probe.The methods enable the detection of M. kansasii more rapidly and with higher accuracy compared with conventional methods performed by culture. Further, the methods can exclude false positive results for the diagnosis and can also detect and diagnose M. kansasii with higher accuracy compared with methods performed by PCR using conventional primers and/or probes. Still further, the method can quantify M. kansasii cells.
    • 本发明公开了一种寡核苷酸,其包含SEQ ID NO:1-4所示的核苷酸序列的部分或全部序列,或与SEQ ID NO:1-4互补的核苷酸序列的部分或全部序列,其中 寡核苷酸能够与堪萨斯分枝杆菌的核苷酸序列杂交; 用于检测包含所述寡核苷酸的堪萨斯(Kansasii)的引物和探针; 以及使用引物和/或探针检测堪萨斯假单胞菌的方法。 与通过培养进行的常规方法相比,该方法能够更快速地检测堪萨斯大肠杆菌。 此外,与使用常规引物和/或探针的PCR进行的方法相比,该方法可以排除诊断的假阳性结果,并且还可以以更高的准确度检测和诊断堪萨斯堪萨斯州。 此外,该方法可以量化堪萨斯群岛细胞。