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    • 94. 发明授权
    • Method and apparatus for performing hydrostatic correction in a pipette
    • 用于在移液管中进行静水校正的方法和装置
    • US5024109A
    • 1991-06-18
    • US477314
    • 1990-02-08
    • Hugo RomeroRobert Anderson
    • Hugo RomeroRobert Anderson
    • B01L3/02G01F11/02G01F25/00
    • G01F11/021B01L3/0227G01F25/0092
    • This invention provides a method and apparatus for correcting for the hydrostatic dead volume error which may occur in a pipette system and may also include method and apparatus for correcting for a lost fluid volume error. The technique involves determining the height of fluid in the tip for a desired fluid volume, determining the change in the dead volume resulting from having fluid of such height in the tip and determining the piston displacement required to achieve a volume equal to the sum of the desired volume and the determined change in dead volume, this determined displacement being the piston movement required to achieve the desired fluid volume. The height is determined from the volume multiplied by values which are constant for a given tip size and geometry. The change in dead volume varies as a function of the desired fluid height times a constant for a given fluid, pipette system and tip. Where a plurality of fluid samples are in the tip, and at least some of the samples are separated by a gas, the height, dead volume change and piston displacement determinations are made for each measured piston displacement to either intake or dispense. For multiple samples, height determinations for each sample are made utilizing only fluid heights. Where appropriate, a lost fluid volume correction value may also be added to the corrected volume utilized to perform piston displacement calculations.
    • 本发明提供了一种用于校正可能在移液管系统中发生的流体静力学死体积误差的方法和装置,并且还可以包括用于校正损失的流体体积误差的方法和装置。 该技术涉及确定尖端中的流体的高度以达到期望的流体体积,确定由于在尖端中具有这种高度的流体而导致的死体积的变化,并确定达到等于 所需体积和确定的死体积的变化,该确定的位移是实现所需流体体积所需的活塞运动。 根据体积乘以给定尖端尺寸和几何的常数值确定高度。 死体积的变化随着给定流体,移液管系统和尖端的期望流体高度乘以常数而变化。 在多个流体样品位于尖端中,并且至少一些样品被气体分离的情况下,对于每个测量的活塞位移进行进入或分配的高度,死体积变化和活塞位移测定。 对于多个样品,仅使用流体高度制备每个样品的高度测定。 在适当的情况下,还可以将损失的流体体积校正值加到用于执行活塞位移计算的校正体积中。