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    • 92. 发明申请
    • Optical scanning microscope with linear scanning
    • 光学扫描显微镜,线性扫描
    • US20060012868A1
    • 2006-01-19
    • US10967345
    • 2004-10-19
    • Ralf Wolleschensky
    • Ralf Wolleschensky
    • G02B21/06
    • G02B21/0032G02B21/0036
    • Optical scanning microscope with linear scanning and with illumination of a specimen via a beam splitter, which is arranged in an objective pupil and consists of at least a reflecting first portion and at least a transmitting second portion, whereby the reflecting portion serves to couple in the illumination light and the transmitting portion serves to pass the detection light in the detection direction or the transmitting portion serves to couple in the illumination light and the reflecting portion serves to couple out the detection light, with a first scanning arrangement, whereby means are provided in the detection light path for the overlay of at least one further scanning arrangement for illumination and detection.
    • 光学扫描显微镜,其具有线性扫描和通过分束器照射样本,该分束器被布置在物镜光瞳中并且由至少反射的第一部分和至少透射的第二部分组成,由此反射部分用于在 照明光和透射部分用于使检测光在检测方向上通过,或者透射部分用于在照明光中耦合,并且反射部分用于以第一扫描装置耦合出检测光,由此装置 用于覆盖用于照明和检测的至少一个另外的扫描装置的检测光路。
    • 93. 发明申请
    • Light raster microscope and its use
    • 光栅显微镜及其用途
    • US20060012867A1
    • 2006-01-19
    • US10967339
    • 2004-10-19
    • Ralf Wolleschensky
    • Ralf Wolleschensky
    • G02B21/06
    • G02B21/0064G02B21/0032
    • In a light raster scanning microscope with a spot illumination arrangement (2) which provides an illumination beam for the illumination of a sample (23) in the form of points or point groups, a scanning arrangement (3, 4) which guides the illumination beam in the form of points or point groups over the sample in a manner so as to scan, a spot detector arrangement (5) which images, via the scanning arrangement (3, 4), the illuminated point or point group spot of the sample (23) by means of at least one confocal aperture (26) on at least one detector unit (28), and a control unit which controls the scanning arrangement (3, 4) and reads out the spot detector arrangement (5), it is provided that, in addition, a wide-field illumination source (29, 34) is provided which illuminates the sample (23) and that the control unit controls the scanning arrangement (3, 4) during the operation of the wide-field illumination source (29, 34) and reads out the spot detector arrangement (5) in such a manner that an image of the sample (23) subject to wide-field illumination is obtained.
    • 在具有点照明装置(2)的光栅扫描显微镜中,该点阵照明装置(2)提供用于以点或点组的形式照射样品(23)的照明光束,引导照明光束的扫描装置(3,4) 以扫描方式在样本上的点或点组的形式,经由扫描装置(3,4)将样本的照亮点或点组点图像(3)进行成像的点检测器装置(5) 至少一个检测器单元(28)上的至少一个共焦孔(26),以及控制扫描装置(3,4)并读出点检测装置(5)的控制单元, 此外,另外还提供了一种照明样品(23)的宽场照明源(29,34),并且在宽场照明源的操作期间控制单元控制扫描装置(3,4) (29,34),并读出点检测器ar (5),以获得经受广场照明的样本(23)的图像。
    • 94. 发明申请
    • Arrangement for microscopic observation and/or detection and use
    • 微观观察和/或检测和使用的布置
    • US20060012866A1
    • 2006-01-19
    • US10967322
    • 2004-10-19
    • Ralf Wolleschensky
    • Ralf Wolleschensky
    • G02B21/06
    • G02B21/002G02B21/082
    • Arrangement for microscopic observation and/or detection of a sample that is at least partially transparent by way of a microscope objective, whereby an illumination of the sample outside the objective is carried out from at least from one side at an angle to the optical axis of the objective and the illumination light is focused on the sample with a smaller aperture than that of the viewing objective and that a coupling of the illumination light over a beam splitter, preferably in the objective pupil, is carried out for coupling, at its circumference, slightly expanding transmitting or reflecting areas for steering the illumination light to the sample, but otherwise is designed so that it is reflecting or transmitting for the sample light on the rest of the area.
    • 用于显微镜观察和/或检测通过显微镜物镜至少部分透明的样品的布置,由此从物镜外部的样品的至少一侧与光轴的一个角度 物镜和照明光聚焦在具有比观察物体小的光圈的样品上,并且在光束分离器(优选在物镜光瞳)中的照明光的耦合被执行以在其圆周处的耦合, 略微扩大用于将照明光转向样品的透射或反射区域,但是否则被设计成使得其在该区域的其余部分上的样品光的反射或透射。
    • 97. 发明授权
    • Arrangement for the optical capture of excited and/or back scattered light beam in a sample
    • 用于光学捕获样品中激发和/或反向散射光束的布置
    • US06947127B2
    • 2005-09-20
    • US10313649
    • 2002-12-06
    • Ralf WolleschenskyMichael Kempe
    • Ralf WolleschenskyMichael Kempe
    • G02B21/00G02B21/06G02B21/16G01N21/00G01J1/42
    • G02B21/0076G02B21/06G02B21/16
    • An arrangement for the optical detection of light radiation which is excited and/or backscattered in a specimen in a microscope, wherein the specimen illumination is focused in and/or in the vicinity of pupil plane of the beam path between the specimen plane and detection plane and apparatus is provided in this plane for spatially separating the illumination light from the detection light, wherein fluorescent light and/or luminescent light and/or phosphorescent light and/or diffusely scattered illumination light coming from the specimen is detected, and the apparatus for spatial separation comprises at least a reflecting first portion and at least a transmitting second portion, wherein the reflecting portion serves to couple in the illumination light and the transmitting portion serves to pass the detection light in the detection direction or the transmitting portion serves to couple in the illumination light and the reflecting portion serves to couple out the detection light.
    • 一种用于在显微镜中在样本中被激发和/或反向散射的光辐射的光学检测装置,其中所述检体照射被聚焦在所述检测平面和检测平面之间的光束路径的光瞳面附近和/或附近 并且在该平面中设置用于将来自检测光的照明光空间分离的装置,其中检测来自样本的荧光和/或发光和/或磷光和/或漫散射的照明光,并且用于空间的装置 分离至少包括反射第一部分和至少透射第二部分,其中反射部分用于在照明光中耦合,并且透射部分用于使检测光在检测方向上通过,或者透射部分用于在 照明光和反射部分用于耦合出检测光。
    • 100. 发明授权
    • Confocal incident-light scanning microscope with means for frequency conversion
    • 共焦点光扫描显微镜,具有变频功能
    • US09588326B2
    • 2017-03-07
    • US14130966
    • 2012-07-05
    • Daniel SchwedtRalf Wolleschensky
    • Daniel SchwedtRalf Wolleschensky
    • G02B26/00G02F1/29G02B21/00G02F1/35
    • G02B21/0076G02B21/0032G02F1/29G02F1/353
    • A microscope which makes possible a spectrally-flexible excitation and detection of fluorescence in an economical manner. For this purpose, means for frequency conversion are arranged in the common beam path and a filter for excitation light is arranged in addition to the main beam splitter in the detection beam path. The frequency conversion achieves a spectral delimitation between illumination light, which is emitted by the light source, and excitation light which brings about fluorescence excitation in the specimen. Because the frequency conversion takes place in the common beam path after the main beam splitter, it is possible for both a spatial separation of illumination light, and excitation light and fluorescent light (detection light) emitted by the specimen, to be carried out in an economical manner at the main beamsplitter according to spectral bands because of the spectral difference between illumination light and excitation light.
    • 一种显微镜,可以以经济的方式进行光谱灵活的荧光激发和检测。 为此,在公共光束路径中布置用于频率转换的装置,并且除了检测光束路径中的主分束器之外还布置有用于激发光的滤光器。 频率转换实现了由光源发射的照明光与在样本中产生荧光激发的激发光之间的光谱定界。 由于在主分束器之后的共同光束路径中发生频率转换,所以可以在照射光的空间分离和由样本发射的激发光和荧光(检测光)两者中进行 由于照明光和激发光之间的光谱差异,根据光谱带在主分束器处经济。