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92. 发明授权

US06461853B1 Method for surface culture of microorganisms and cells in flexible culture bags 失效
标题翻译：柔性培养袋中微生物和细胞表面培养的方法
公开(公告)号：US06461853B1
公开(公告)日：2002-10-08
申请号：US09858451
申请日：2001-05-17
申请人： Hong Zhu
发明人： Hong Zhu
IPC分类号： C12N100
CPC分类号： C12M23/14 , C12M23/46 , C12M23/48 , C12N1/00
摘要： A method of using flexible bags for surface culture of microorganisms and cells on a stationary interface between a gaseous phase and a liquid medium and the devices upon which the method are practiced are disclosed. A flexible culture bag is first filled with a liquid growth medium, a sample inoculum, and a predetermined amount of air to create a headspace. The bag is then sealed and placed on a stationary platform, and mounted with an external self-support device on the top web of the bag. The external support device holds the top web of the bag in a fixed position and, thus, prevents the bag from deflating so that a stationary interface between the headspace and the liquid medium in the bag is maintained throughout the cultivation period.
摘要翻译：公开了一种在气相和液体介质之间的固定界面上使用柔性袋用于表面培养微生物和细胞的方法以及实施该方法的装置。首先将柔性培养袋填充液体生长培养基，样品接种物和预定量的空气以产生顶部空间。然后将袋密封并放置在固定的平台上，并在袋的顶部网上安装有外部自支撑装置。外部支撑装置将袋的顶部网保持在固定位置，并且因此防止袋放气，使得整个培养期间保持袋中的顶部空间和液体介质之间的固定界面。

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