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    • 1. 发明授权
    • Method for determining the coagulation potential of a plasma sample
    • 确定血浆样品凝固电位的方法
    • US06838251B1
    • 2005-01-04
    • US09601073
    • 1999-02-01
    • Thomas Exner
    • Thomas Exner
    • G01N33/86C12Q1/56
    • C12Q1/56G01N2333/462
    • A method of determining the coagulation potential of a plasma sample be pre-incubating the plasma sample with a reagent such that endogenous protein C in the plasma is at least partially converted into activated protein C by the reagent, adding factor Xa which is progressively inactivated by antithrombin III/heparin cofactor 2 during the preincubation, adding an exogenous reagent which activates factor X to Xa or prothrombin to thrombin in a factor V-dependent manner, monitoring a reaction indicative of the rate of coagulation of the plasma sample, comparing that rate of coagulation control, or the equivalent rate determined for an individual without impaired coagulation control, or the equivalent rate determined for the plasma sample in the absence of protein C activator, and determining the coagulation potential of the plasma sample from one or other of the compairsons.
    • 确定血浆样品的凝血电位的方法是用试剂预先培养血浆样品,使得血浆中的内源性蛋白C至少部分地被试剂转化成活化的蛋白质C,加入通过下述方法逐渐失活的添加因子Xa 抗凝血酶III /肝素辅因子2,加入外因试剂,以因子V依赖性方式将X因子激活至Xa或凝血酶凝血酶,监测血浆样品凝血速率的反应, 凝血控制或对于没有受损凝血控制的个体而确定的等效速率,或者在不存在蛋白C激活剂的情况下为血浆样品确定的等效速率,以及确定来自一个或其它比较物的血浆样品的凝固电位。