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1. 发明申请

US20050125849A1 Stem cell-specific promoters and their use 有权
标题翻译：干细胞特异性启动子及其用途
公开(公告)号：US20050125849A1
公开(公告)日：2005-06-09
申请号：US10974265
申请日：2004-10-27
申请人： Sergey Shmelkov , Shahin Rafii , Jun Lin
发明人： Sergey Shmelkov , Shahin Rafii , Jun Lin
IPC分类号： C12N15/85 , A01K67/027 , C07H21/04 , C12N5/08 , C12N9/10 , C12Q1/68
CPC分类号： C12N15/85 , A01K2217/05 , C12N2830/008 , C12N2830/46
摘要： The present invention relates to a method of isolating stem cells from a mixed population of different cell types. This method involves selecting a promoter which functions only in said stem cells and not in the other cell types. The promoter can have a nucleotide sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, and SEQ ID NO: 8. A nucleic acid molecule encoding a marker protein under control of said promoter is introduced into all cell types of said mixed population and only the stem cells, but not the other cell types, within said mixed population are allowed to express said marker protein. The cells of said mixed population of cell types that are expressing the marker protein, which are restricted to the stem cells are identified and separated from the mixed population, where the separated cells are restricted to the stem cells. The promoter, nucleic acid constructs comprising it, and the resulting stem cells are also disclosed. The nucleic constructs can be incorporated in transgenic animals in order to monitor stem cell movement. A disorder mediated by stem cell proliferation can be carried out by administering both (1) a nucleic acid construct comprising the promoter of the present application and an enzyme capable of converting a prodrug to a cytotoxic drug and (2) the prodrug.
摘要翻译：本发明涉及从不同细胞类型的混合群体中分离干细胞的方法。该方法包括选择仅在所述干细胞中起作用但不在其它细胞类型中起作用的启动子。启动子可以具有选自SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO：7和SEQ ID NO：8的核苷酸序列。将所述启动子控制下的标记蛋白引入所述混合群体的所有细胞类型中，并且仅允许所述混合群体内的干细胞，而不是其他细胞类型表达所述标记蛋白。将所述混合的表达限制于干细胞的标记蛋白的细胞类型的细胞群从混合群体中鉴定和分离，其中分离的细胞限于干细胞。还公开了启动子，包含其的核酸构建体和所得干细胞。核酸构建体可以并入转基因动物中以监测干细胞运动。通过施用（1）包含本申请的启动子的核酸构建体和能够将前药转化为细胞毒性药物的酶和（2）前药，可以进行由干细胞增殖介导的病症。

2. 发明授权

US07396680B2 Stem cell-specific promoters and their use 有权
标题翻译：干细胞特异性启动子及其用途
公开(公告)号：US07396680B2
公开(公告)日：2008-07-08
申请号：US10974265
申请日：2004-10-27
申请人： Sergey V. Shmelkov , Shahin Rafii , Jun Lin
发明人： Sergey V. Shmelkov , Shahin Rafii , Jun Lin
IPC分类号： C12N15/00 , C12N5/02
CPC分类号： C12N15/85 , A01K2217/05 , C12N2830/008 , C12N2830/46
摘要： The present invention relates to a method of isolating stem cells from a mixed population of different cell types. This method involves selecting a promoter which functions only in said stem cells and not in the other cell types. The promoter can have a nucleotide sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, and SEQ ID NO: 8. A nucleic acid molecule encoding a marker protein under control of said promoter is introduced into all cell types of said mixed population and only the stem cells, but not the other cell types, within said mixed population are allowed to express said marker protein. The cells of said mixed population of cell types that are expressing the marker protein, which are restricted to the stem cells are identified and separated from the mixed population, where the separated cells are restricted to the stem cells. The promoter, nucleic acid constructs comprising it, and the resulting stem cells are also disclosed. The nucleic constructs can be incorporated in transgenic animals in order to monitor stem cell movement. A disorder mediated by stem cell proliferation can be carried out by administering both (1) a nucleic acid construct comprising the promoter of the present application and an enzyme capable of converting a prodrug to a cytotoxic drug and (2) the prodrug.
摘要翻译：本发明涉及从不同细胞类型的混合群体中分离干细胞的方法。该方法包括选择仅在所述干细胞中起作用但不在其它细胞类型中起作用的启动子。启动子可以具有选自SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO：7和SEQ ID NO：8的核苷酸序列。将所述启动子控制下的标记蛋白引入所述混合群体的所有细胞类型中，并且仅允许所述混合群体内的干细胞，而不是其他细胞类型表达所述标记蛋白。将所述混合的表达限制于干细胞的标记蛋白的细胞类型的细胞群从混合群体中鉴定和分离，其中分离的细胞限于干细胞。还公开了启动子，包含其的核酸构建体和所得干细胞。核酸构建体可以并入转基因动物中以监测干细胞运动。通过施用（1）包含本申请的启动子的核酸构建体和能够将前药转化为细胞毒性药物的酶和（2）前药，可以进行由干细胞增殖介导的病症。

3. 发明授权

US08974898B2 Coated substrate having enhanced scratch and mar resistance 有权
标题翻译：涂层基材具有增强的耐擦伤性和耐擦伤性
公开(公告)号：US08974898B2
公开(公告)日：2015-03-10
申请号：US11818460
申请日：2007-06-14
申请人： Jun Lin
发明人： Jun Lin
IPC分类号： B32B5/16 , B32B9/04 , B32B15/08 , C09D7/12 , B82Y30/00 , C09C1/30 , C09D133/06 , C09D143/04 , C09D201/02 , C08K3/36 , C08K5/00 , C08K9/04 , C08K9/08
CPC分类号： C09D7/1225 , B82Y30/00 , C01P2004/62 , C01P2004/64 , C08K3/36 , C08K5/0025 , C08K9/04 , C08K9/08 , C09C1/3081 , C09D7/62 , C09D7/67 , C09D7/68 , C09D7/69 , C09D133/066 , C09D143/04 , C09D201/02 , Y10T428/25 , Y10T428/254 , Y10T428/31663 , Y10T428/31692
摘要： Disclosed herein is a substrate comprising a coating composition of silica nano-particles having enhanced mar and scratch resistance. In particular, the invention relates to a coating composition that contains silica nano-particle dispersion for use in automotive coating applications.
摘要翻译：本文公开了一种基材，其包含具有增强的耐划痕性和耐擦伤性的二氧化硅纳米颗粒的涂料组合物。特别地，本发明涉及包含用于汽车涂料应用的二氧化硅纳米颗粒分散体的涂料组合物。

4. 发明申请

US20140375765A1 Method and system for transmitting 3D picture in multimedia message service message 审中-公开
标题翻译：用于在多媒体消息服务消息中传输3D图像的方法和系统
公开(公告)号：US20140375765A1
公开(公告)日：2014-12-25
申请号：US14344940
申请日：2011-11-01
申请人： Wen Fang , Yongqi Li , Jun Lin
发明人： Wen Fang , Yongqi Li , Jun Lin
IPC分类号： H04W4/12 , H04N13/00
CPC分类号： H04W4/12 , H04N13/178 , H04N13/194 , H04N21/235 , H04N21/431 , H04N21/435 , H04N21/4786 , H04N21/8153 , H04N21/8543
摘要： A method for transmitting a three-dimensional (3D) picture in an MMS message is disclosed. The method includes: in a Synchronized Multimedia Integration Language (SMIL) file, a sender assigns a same Identifier (ID) for two different pictures, sets an attribute of each of the two pictures as a 3D display picture, and sends the two pictures to a receiver; or, a sender synthesizes two different pictures in the SMIL file into a file, assigns an ID for the synthesized file, sets an attribute of the ID as a 3D display picture, and sends the synthesized file to a receiver; and the receiver displays the two pictures in a 3D mode. A system for transmitting a 3D picture in an MMS message is also disclosed. With the technical solution of the disclosure, the problem that transmission of a 3D picture cannot be supported in an existing protocol can be solved.
摘要翻译：公开了一种用于在MMS消息中发送三维（3D）图像的方法。该方法包括：在同步多媒体集成语言（SMIL）文件中，发送者为两个不同的图片分配相同的标识符（ID），将两个图像中的每一个的属性设置为3D显示图像，并将两个图像发送到接收器或者，发送者将SMIL文件中的两个不同图片合成为文件，为合成文件分配ID，将ID的属性设置为3D显示图片，并将合成文件发送给接收者; 并且接收器以3D模式显示两张照片。还公开了一种用于在MMS消息中发送3D图像的系统。通过本公开的技术方案，可以解决现有协议中不能支持3D图像传输的问题。

5. 发明授权

US08784555B2 Surface active blocked isocyanates and coating compositions thereof 有权
标题翻译：表面活性封闭异氰酸酯及其涂料组合物
公开(公告)号：US08784555B2
公开(公告)日：2014-07-22
申请号：US13054828
申请日：2009-07-23
申请人： Jun Lin
发明人： Jun Lin
IPC分类号： C09D7/00 , C07F7/18
CPC分类号： C09D7/00 , C07F7/0838 , C07F7/18 , C08G77/045 , C08G77/26 , C09D4/00 , C09D183/04 , C09J183/04
摘要： Disclosed herein is a surface active adhesion promoting agent and a coating composition comprising the surface active adhesion promoting agent. The adhesion promoting agent is a blocked isocyanate functional siloxane containing material. Addition of small amount of the adhesion promoting agent to a coating composition, especially clearcoat compositions, helps to provide adhesion to a variety of materials that may be subsequently applied to the coating composition.
摘要翻译：本文公开了表面活性粘合促进剂和包含表面活性粘合促进剂的涂料组合物。粘合促进剂是封端的异氰酸酯官能的含硅氧烷的材料。向涂料组合物，特别是透明涂料组合物中加入少量的粘合促进剂有助于提供对可随后施用于涂料组合物的各种材料的粘附。

6. 发明申请

US20110207852A1 SELF-ASSEMBLED SILICA CONDENSATES 有权
标题翻译：自组装二氧化硅凝结剂
公开(公告)号：US20110207852A1
公开(公告)日：2011-08-25
申请号：US13126524
申请日：2009-07-28
申请人： Jun Lin
发明人： Jun Lin
IPC分类号： C09D183/00 , C09D163/00 , B05D3/02
CPC分类号： C09D183/04 , C08G18/6295 , C08G77/14 , C08G77/20 , C08G77/26 , C08K3/36 , C08L2312/00 , C09D133/04 , C09D175/04
摘要： Self-assembled silica condensates are described as well as their use in coating compositions. The self-assembled silica condensates can be formed from the hydrolysis of medium to long chain trialkoxy silane compounds. Coating compositions containing the self-assembled silica condensates can provide coatings having improved scratch and mar resistance and can have excellent recoat adhesion.
摘要翻译：描述了自组装二氧化硅冷凝物以及它们在涂料组合物中的用途。自组装二氧化硅缩合物可以由中长链三烷氧基硅烷化合物的水解形成。含有自组装二氧化硅冷凝物的涂料组合物可以提供具有改善的耐划痕性和抗划痕性的涂层，并且可以具有优异的重涂层附着力。

7. 发明授权

US07947821B2 Optimized DNA sequences encoding recombinant human bone morphogenetic protein-2 (rhBMP-2), preparation method and the uses thereof 有权
标题翻译：编码重组人骨形态发生蛋白-2（rhBMP-2）的优化DNA序列，其制备方法及其用途
公开(公告)号：US07947821B2
公开(公告)日：2011-05-24
申请号：US12623527
申请日：2009-11-23
申请人： Changshen Liu , Jun Lin , Jiangchao Qian , Yuan Yuan
发明人： Changshen Liu , Jun Lin , Jiangchao Qian , Yuan Yuan
IPC分类号： C07H21/02 , C07H21/04 , C12N15/63 , C12N15/85 , C12N15/86 , C12N1/20
CPC分类号： C07K14/51 , A61K38/00
摘要： The invention discloses an optimized DNA sequence of recombinant human bone morphogenetic protein-2 (rhBMP-2) based on the Escherichia coli expression system and a method for the preparation of the rhBMP-2. Specifically, the invention provides the optimal DNA sequences suitable for Escherichia coli expression system, the methods for efficient preparation of the rhBMP-2, and the related construction of the recombinant bacteria, the expression and purification technologies. Compared with the traditional hBMP-2 gene without optimizing, the rhBMP-2 expression level of the optimized gene in Escherichia coli is increased by 50%. Additionally, this invention also provides a method for preparation long chain rhBMP-2 with enhanced renaturation efficiency and yield of purification.
摘要翻译：本发明公开了基于大肠杆菌表达系统的重组人骨形态发生蛋白-2（rhBMP-2）的优化DNA序列和rhBMP-2的制备方法。具体而言，本发明提供了适用于大肠杆菌表达系统的最佳DNA序列，rhBMP-2的有效制备方法，以及重组细菌的相关构建，表达和纯化技术。与未经优化的传统hBMP-2基因相比，大肠杆菌中优化基因的rhBMP-2表达水平提高了50％。此外，本发明还提供了具有增强的复性效率和纯化产率的长链rhBMP-2的制备方法。

8. 发明申请

US20100331250A1 ANTI-ANGIOGENESIS FUSION PROTEINS 有权
标题翻译：抗血栓形成融合蛋白
公开(公告)号：US20100331250A1
公开(公告)日：2010-12-30
申请号：US12727188
申请日：2010-03-18
申请人： Joe ZHOU , Jianyang Zhao , Jiuru Sun , Jun Wang , Shaoxiong Wang , Xinxing Ma , Jun Lin
发明人： Joe ZHOU , Jianyang Zhao , Jiuru Sun , Jun Wang , Shaoxiong Wang , Xinxing Ma , Jun Lin
IPC分类号： A61K38/18 , C12P21/06 , C12N5/10 , C12N1/19 , C12N15/63 , C07K14/705 , C07K16/22 , C07H21/04
CPC分类号： C07K14/71 , A61K38/00 , C07K2319/30
摘要： The disclosure provides a novel anti-angiogenesis fusion protein. The present invention combines a chimeric vascular endothelial cell growth factor (VEGF) receptor or a fragment thereof with a multimerizing component, which have a superior binding capacity with human VEGF and placental growth factor (PIGF). The fusion protein has improved stability, prolonged half-life and the ability to form multivalent interactions with VEGF, and can be used for anti-angiogenesis, treating VEGF related diseases and inhibiting tumor growth.
摘要翻译：本公开提供了一种新的抗血管生成融合蛋白。本发明将嵌合血管内皮细胞生长因子（VEGF）受体或其片段与多聚化成分组合，其与人VEGF和胎盘生长因子（PIGF）具有优异的结合能力。融合蛋白具有改善的稳定性，延长的半衰期和与VEGF形成多价相互作用的能力，并且可用于抗血管生成，治疗VEGF相关疾病和抑制肿瘤生长。

9. 发明授权

US07838078B2 Clearcoat composition compatible with both waterborne and solventborne basecoats 有权
标题翻译：透明涂料组合物与水性和溶剂型底漆兼容
公开(公告)号：US07838078B2
公开(公告)日：2010-11-23
申请号：US10929239
申请日：2004-08-30
申请人： Jun Lin
发明人： Jun Lin
IPC分类号： B05D1/36
CPC分类号： C08G18/792 , C08G18/2825 , C09D175/04 , C09D201/025 , C09D201/10 , C08L2666/16
摘要： A curable film-forming coating composition, typically a clearcoat, having improved compatibility over waterborne and solventborne basecoats. The composition includes a film-forming binder comprising a carbamate material, a curing agent, typically a monomeric melamine curing agent, and a hydroxy functional silane component. When used as a clearcoat over a standard pigmented basecoat, the resulting coating provides a substantially wrinkle free appearance and excellent adhesion to both waterborne and solventborne basecoats.
摘要翻译：一种可固化的成膜涂料组合物，通常为透明涂料，具有改善的相对于水性和溶剂型底涂层的相容性。组合物包括含有氨基甲酸酯材料，固化剂，通常为单体三聚氰胺固化剂和羟基官能的硅烷组分的成膜粘合剂。当用作标准着色底漆时的透明涂层时，所得涂层提供基本上无皱纹的外观和对水性和溶剂型底涂层的优异粘合性。

10. 发明授权

US07600592B2 Variable configuration articulated tracked vehicle 有权
标题翻译：可变配置铰接跟踪车辆
公开(公告)号：US07600592B2
公开(公告)日：2009-10-13
申请号：US11498981
申请日：2006-08-04
申请人： Andrew A. Goldenberg , Jun Lin
发明人： Andrew A. Goldenberg , Jun Lin
IPC分类号： B62D55/02
CPC分类号： B62D55/075 , B62D55/10 , B62D55/12 , B62D55/14
摘要： A variable configuration articulated tracked vehicle comprises a chassis, a pair of right and left drive pulleys, a right and left planetary wheel, a right and left track, a right and left arm mechanism, and a right and left drive motor. The drive pulleys are rotatably attached to the chassis and each pair of drive pulleys is in the same plane. The planetary wheels are movable relative to the chassis such that each planetary wheel is in the same plane as its respective drive pulleys. The tracks extend around the pair of drive pulleys and the planetary wheel on the respective sides. The arm mechanisms connect the respective planetary wheel to the chassis. Each arm mechanism is rotatably attached to the chassis with a cam. The cam defines a motion path of one end of the arm whereby the motion of the planetary wheel provides a path for the planetary wheel such that the track path defined by the respective drive pulleys and the planetary wheel is a constant track length. The motors are each operably connected to the respective pair of drive pulleys for driving the track.
摘要翻译：可变配置的铰接跟踪车辆包括底架，左右一对驱动滑轮，左右行星轮，左右轨道，右侧和左侧臂机构以及左右驱动马达。驱动滑轮可旋转地附接到底盘，并且每对驱动滑轮在同一平面内。行星轮相对于底盘可移动，使得每个行星轮与其相应的驱动滑轮处于同一平面。轨道围绕该对驱动滑轮和相应侧面上的行星轮延伸。臂机构将相应的行星轮连接到底盘。每个臂机构用凸轮可旋转地附接到底盘。凸轮限定臂的一端的运动路径，由此行星轮的运动为行星轮提供路径，使得由相应的驱动滑轮和行星轮限定的轨道路径是恒定的轨道长度。电动机各自可操作地连接到相应的一对驱动滑轮，用于驱动轨道。

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