专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20130108590A1 METHOD FOR PRODUCING CELL SHEET 审中-公开
标题翻译：生产细胞片的方法
公开(公告)号：US20130108590A1
公开(公告)日：2013-05-02
申请号：US13697180
申请日：2011-05-10
申请人： Masayo Takahashi , Tsutomu Yasukawa
发明人： Masayo Takahashi , Tsutomu Yasukawa
IPC分类号： C12N5/071
CPC分类号： C12N5/0602 , A61L27/3808 , A61L27/3869 , A61L2430/16
摘要： The present invention provides a method for producing a cell sheet, including(1) a step of preparing mammal-derived cells, and (2) a step of seeding the prepared cells on a plane substrate in high density, and culturing the cells, a cell sheet produced by the method, a material for transplantation, Bruch's membrane and the like.
摘要翻译：本发明提供一种细胞片的制造方法，其包括（1）制备哺乳动物来源的细胞的步骤，（2）将制备的细胞以高密度接种在平面基质上并培养细胞的步骤通过该方法生产的细胞片，用于移植的材料，Bruch's膜等。

2. 发明申请

US20070037282A1 Method of producing lens cell and lens cell obtained by the method 审中-公开
标题翻译：通过该方法获得的透镜细胞和透镜细胞的制造方法
公开(公告)号：US20070037282A1
公开(公告)日：2007-02-15
申请号：US10551378
申请日：2004-03-22
申请人： Masayo Takahashi , Yoshiki Sasai , Hiroshi Kawasaki , Sotaro Ooto
发明人： Masayo Takahashi , Yoshiki Sasai , Hiroshi Kawasaki , Sotaro Ooto
IPC分类号： C12N5/08
CPC分类号： C12N5/0621 , C12N2501/115 , C12N2506/02
摘要： A method of producing a lens cell is disclosed. The method includes: an ES cell maintenance step of maintaining an ES cell by using a medium containing a fibroblast growth factor FGF-2 at a concentration of 2 ng/ml to 50 ng/ml; and a differentiation inducing step, carried out after the ES cell maintenance step, of inducing differentiation of the ES cell into a lens cell by implanting and culturing the ES cell on a mouse-skull-cell PA6 at a cell density of 2 colonies/cm2 to 6.5 colonies/cm2. In one embodiment, a washing step, carried out between the ES cell maintenance step and the differentiation inducing step, of washing the maintained ES cell once with an ES differentiation medium is included.
摘要翻译：公开了一种制造透镜单元的方法。该方法包括：通过使用浓度为2ng / ml至50ng / ml的含有成纤维细胞生长因子FGF-2的培养基来维持ES细胞的ES细胞维持步骤; 以及在ES细胞维持步骤之后进行的分化诱导步骤，通过以2个菌落/ cm 2的细胞密度将ES细胞植入和培养到小鼠颅骨细胞PA6上来诱导ES细胞分化成晶状体细胞 2至6.5个菌落/ cm 2。在一个实施方案中，包括在ES细胞维持步骤和分化诱导步骤之间进行的洗涤步骤，用ES分化培养基洗涤维持的ES细胞一次。

3. 发明授权

US10519422B2 Method of producing human retinal pigment epithelial cells 有权
公开(公告)号：US10519422B2
公开(公告)日：2019-12-31
申请号：US13408642
申请日：2012-02-29
申请人： Masayo Takahashi , Satoshi Okamoto , Noriko Sakai
发明人： Masayo Takahashi , Satoshi Okamoto , Noriko Sakai
IPC分类号： C12N5/079 , A61K35/30
摘要： The invention relates to a method of producing a retinal pigment epithelial cell from a human pluripotent stem cell, and a method of treating or preventing a retinal disease by using the produced cell. The retinal pigment epithelial cell is prepared by (a) inducing differentiation of a human pluripotent stem cell into a pigment cell by adhesion cultivation of a human pluripotent stem cell in a medium containing a Nodal signal inhibitor and a Wnt signal inhibitor in the absence of a feeder cell to give a culture containing the pigment cell, (b) subjecting the obtained culture to further adhesion culture to give a culture containing a pigment cell colony, and (c) isolating the pigment cell from the obtained culture and culturing the cell to give a retinal pigment epithelial cell.

4. 发明申请

US20140057281A1 METHOD OF PRODUCING RETINAL PIGMENT EPITHELIAL CELL SHEET 有权
标题翻译：生产细胞色素上皮细胞片的方法
公开(公告)号：US20140057281A1
公开(公告)日：2014-02-27
申请号：US14001108
申请日：2012-02-24
申请人： Masayo Takahashi , Satoshi Okamoto , Hiroyuki Kamao
发明人： Masayo Takahashi , Satoshi Okamoto , Hiroyuki Kamao
IPC分类号： C12N5/079 , G01N33/50
CPC分类号： C12N5/0621 , A61K35/00 , C12N2506/02 , C12N2506/45 , C12N2509/00 , C12N2533/54 , G01N33/5005
摘要： The present invention provides a method of producing a cell sheet including the following steps (1) seeding and culturing retinal pigment epithelial cells on a collagen gel to form a cell sheet composed of the retinal pigment epithelial cells, and (2) degrading the collagen gel with collagenase to detach the cell sheet composed of the retinal pigment epithelial cells, and the like.
摘要翻译：本发明提供一种生产细胞片的方法，包括以下步骤（1）将胶原凝胶上的视网膜色素上皮细胞接种培养以形成由视网膜色素上皮细胞组成的细胞片，和（2）降解胶原凝胶用胶原酶分离由视网膜色素上皮细胞组成的细胞片等。

5. 发明授权

US09902933B2 Method of producing retinal pigment epithelial cell sheet 有权
公开(公告)号：US09902933B2
公开(公告)日：2018-02-27
申请号：US14001108
申请日：2012-02-24
申请人： Masayo Takahashi , Satoshi Okamoto , Hiroyuki Kamao
发明人： Masayo Takahashi , Satoshi Okamoto , Hiroyuki Kamao
IPC分类号： C12N5/079 , G01N33/50 , A61K35/00
CPC分类号： C12N5/0621 , A61K35/00 , C12N2506/02 , C12N2506/45 , C12N2509/00 , C12N2533/54 , G01N33/5005
摘要： The present invention provides a method of producing a cell sheet including the following steps(1) seeding and culturing retinal pigment epithelial cells on a collagen gel to form a cell sheet composed of the retinal pigment epithelial cells, and(2) degrading the collagen gel with collagenase to detach the cell sheet composed of the retinal pigment epithelial cells, and the like.

6. 发明授权

US06767738B1 Method of isolating adult mammalian CNS-derived progenitor stem cells using density gradient centrifugation 失效
标题翻译：使用密度梯度离心法分离成年哺乳动物CNS来源的祖细胞干细胞的方法
公开(公告)号：US06767738B1
公开(公告)日：2004-07-27
申请号：US09913192
申请日：2002-02-12
申请人： Fred H. Gage , Theo Palmer , Francis G. Safar , Jun Takahashi , Masayo Takahashi
发明人： Fred H. Gage , Theo Palmer , Francis G. Safar , Jun Takahashi , Masayo Takahashi
IPC分类号： C12N502
CPC分类号： C12N5/0623 , A61K35/12 , A61K48/00 , C12N2501/115 , C12N2510/00
摘要： The present invention is directed to methods of repairing damaged or diseased, specialized or differentiated tissue in mature animals, particularly neuronal tissue such as retinas. In particular, the invention relates to transplantation of adult, hippocampus-derived progenitor cells into a selected neural tissue site of a recipient. These cells can functionally integrate into mature and immature neural tissue. The invention encompasses, in one aspect, repopulating a retina of a dystrophic animal with neurons, by injecting clonally derived, adult central nervous system derived stem cells (ACSC) derived from a healthy donor animal into an eye of the dystrophic recipient. Herein disclosed is the first successful and stable integration of clonally derived ACSC into same-species but different strain recipients (e. g., Fischer rat-derived adult hippocampal derived progenitor cells (AHPCs) into dystrophic RCS rats). Surprisingly, AHPCs were also found to integrate successfully into a xenogeneic recipient (e.g., rat AHPCs into the retina of dystropic rd-I mice).
摘要翻译：本发明涉及修复成熟动物特别是神经元组织如视网膜的受损或患病，专门或分化组织的方法。特别地，本发明涉及将成年的海马衍生的祖细胞移植到接受者的所选择的神经组织部位。这些细胞可以功能整合到成熟和未成熟的神经组织中。本发明在一个方面包括通过将衍生自健康供体动物的克隆衍生的成体中枢神经系统干细胞（ACSC）注射到营养不良的接受者的眼睛中，将营养不良的动物的视网膜与神经元重新填充。本文公开了克隆衍生的ACSC在相同物种但不同的菌株接收者（例如，Fischer大鼠衍生的成年海马衍生的祖细胞（AHPC））到营养不良的RCS大鼠中的第一次成功和稳定的整合。令人惊讶的是，还发现AHPC成功地融合到异种受体（例如，大鼠AHPC进入dystropic rd-I小鼠的视网膜）中。

7. 发明授权

US09133435B2 Method for induction/differentiation into photoreceptor cell 有权
标题翻译：诱导/分化成感光细胞的方法
公开(公告)号：US09133435B2
公开(公告)日：2015-09-15
申请号：US12523444
申请日：2008-01-11
申请人： Masayo Takahashi , Fumitaka Osakada , Michiko Mandai , Hanako Ikeda
发明人： Masayo Takahashi , Fumitaka Osakada , Michiko Mandai , Hanako Ikeda
IPC分类号： C12N15/00 , C12N5/0793 , C12N5/079 , C12N5/0797 , A61K35/12
CPC分类号： C12N5/062 , A61K35/12 , C12N5/0621 , C12N5/0623 , C12N2500/33 , C12N2500/90 , C12N2500/99 , C12N2501/113 , C12N2501/115 , C12N2501/16 , C12N2501/385 , C12N2501/415 , C12N2501/999 , C12N2506/02 , C12N2506/08 , C12N2533/00
摘要： The present invention provides a method of producing primate retinal progenitor cells, comprising culturing primate embryonic stem cells as suspended aggregates in a serum-free medium, and obtaining retinal progenitor cells from the culture. The present invention further provides a method of producing photoreceptor precursor cells, comprising culturing isolated retinal progenitor cells differentiated from embryonic stem cells, under adhesive conditions, in the presence of a gamma secretase inhibitor, and obtaining a photoreceptor precursor from the culture.
摘要翻译：本发明提供了一种产生灵长类动物视网膜祖细胞的方法，包括在无血清培养基中培养灵长类动物胚胎干细胞作为悬浮聚集体，并从培养物中获得视网膜祖细胞。本发明还提供了一种制备感光体前体细胞的方法，包括在粘附条件下，在γ分泌酶抑制剂的存在下培养与胚胎干细胞分化的分离的视网膜祖细胞，并从培养物中获得光感受器前体。

8. 发明申请

US20100105137A1 METHOD FOR INDUCTION/DIFFERENTIATION INTO PHOTORECEPTOR CELL 有权
标题翻译：用于诱导/分化成光电子细胞的方法
公开(公告)号：US20100105137A1
公开(公告)日：2010-04-29
申请号：US12523444
申请日：2008-01-11
申请人： Masayo Takahashi , Fumitaka Osakada , Michiko Mandai , Hanako Ikeda
发明人： Masayo Takahashi , Fumitaka Osakada , Michiko Mandai , Hanako Ikeda
IPC分类号： C12N5/0735
CPC分类号： C12N5/062 , A61K35/12 , C12N5/0621 , C12N5/0623 , C12N2500/33 , C12N2500/90 , C12N2500/99 , C12N2501/113 , C12N2501/115 , C12N2501/16 , C12N2501/385 , C12N2501/415 , C12N2501/999 , C12N2506/02 , C12N2506/08 , C12N2533/00
摘要： The present invention provides a method of producing primate retinal progenitor cells, comprising culturing primate embryonic stem cells as suspended aggregates in a serum-free medium, and obtaining retinal progenitor cells from the culture. The present invention further provides a method of producing photoreceptor precursor cells, comprising culturing isolated retinal progenitor cells differentiated from embryonic stem cells, under adhesive conditions, in the presence of a gamma secretase inhibitor, and obtaining a photoreceptor precursor from the culture.
摘要翻译：本发明提供了一种产生灵长类动物视网膜祖细胞的方法，包括在无血清培养基中培养灵长类动物胚胎干细胞作为悬浮聚集体，并从培养物中获得视网膜祖细胞。本发明还提供了一种制备感光体前体细胞的方法，包括在粘附条件下，在γ分泌酶抑制剂的存在下培养与胚胎干细胞分化的分离的视网膜祖细胞，并从培养物中获得光感受器前体。

9. 发明申请

US20090203134A1 REMEDY FOR NERVE CELL REGENERATION 审中-公开
标题翻译：补救神经细胞再生
公开(公告)号：US20090203134A1
公开(公告)日：2009-08-13
申请号：US11814157
申请日：2006-01-17
申请人： Masayo Takahashi , Michiko Mandai , Sotaro Ooto , Fumitaka Osakada
发明人： Masayo Takahashi , Michiko Mandai , Sotaro Ooto , Fumitaka Osakada
IPC分类号： C12N5/06 , C07K14/47
CPC分类号： A61K31/00 , A61K38/1709
摘要： Wnt3a proteins represented by SEQ ID NO:2, etc. are used as the active ingredient of remedy for nerve cell regeneration, in particular, remedy for retinal cell regeneration which is useful in treating retinitis pigmentosa, age-related macular degeneration, cone dystrophy and so on.
摘要翻译：用于由SEQ ID NO：2表示的Wnt3a蛋白等用作神经细胞再生的补救活性成分，特别是可用于治疗色素性视网膜炎，年龄相关性黄斑变性，锥形营养不良的视网膜细胞再生的治疗方法所以。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式