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1. 发明授权

US08415116B2 Method for pre-treating specimen and method for analyzing specimen 失效
公开(公告)号：US08415116B2
公开(公告)日：2013-04-09
申请号：US12113795
申请日：2008-05-01
申请人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu , Kazuhiro Ban
发明人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu , Kazuhiro Ban
IPC分类号： C12Q1/02 , G01N1/30 , G01N1/00
CPC分类号： G01N1/30 , G01N1/31 , G01N2001/282 , G01N2001/317
摘要： A pre-treatment method for analyzing a specified portion in a specimen composed of a biological tissue includes the steps of preparing a specimen to be analyzed, determining a specified portion in the specimen; and applying an analysis inhibitor to a portion except the specified portion of the specimen using a droplet spray method.

2. 发明申请

US20080179512A1 METHOD FOR OBTAINING INFORMATION AND DEVICE THEREFOR 有权
标题翻译：获取信息的方法及其设备
公开(公告)号：US20080179512A1
公开(公告)日：2008-07-31
申请号：US12017584
申请日：2008-01-22
申请人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
发明人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
IPC分类号： B01D59/44
CPC分类号： H01J49/142
摘要： A method for obtaining information on a mass of an object by time-of-flight mass spectrometry. This method includes placing colloidal metal particles for promoting ionization of the object inside the object at a depth ranging from 0.1 nm to 100 nm in opposition to a primary beam for the ionization; irradiating the object with the primary beam selected from the group of ions, neutral particles, and electrons, which can be focused, pulsed, and are capable of scanning, and laser beams, which can be focused, pulsed, and are capable of scanning to ionize a constituent of the object and to allow the ionized constituent to fly out of the object; and obtaining information on the mass of the flying constituent of the object by time-of-flight mass spectrometry.
摘要翻译：一种通过飞行时间质谱法获得物体质量信息的方法。该方法包括将胶体金属颗粒放置在与离子化的主光束相反的0.1nm至100nm范围内的物体内部物体的离子化; 用可以被聚焦，脉冲和能够扫描的离子，中性粒子和电子组中选择的主要束照射物体，以及可以被聚焦，脉冲且能够扫描的激光束电离物体的成分并使离子化成分从物体飞出; 并通过飞行时间质谱法获得关于物体飞行成分质量的信息。

3. 发明授权

US07560689B2 High-sensitivity mass spectrometer and method 失效
标题翻译：高灵敏度质谱仪及方法
公开(公告)号：US07560689B2
公开(公告)日：2009-07-14
申请号：US11682500
申请日：2007-03-06
申请人： Kazuhiro Ban , Hiroyuki Hashimoto , Manabu Komatsu , Norihiko Utsunomiya , Yohei Murayama
发明人： Kazuhiro Ban , Hiroyuki Hashimoto , Manabu Komatsu , Norihiko Utsunomiya , Yohei Murayama
IPC分类号： B01D59/44 , H01J49/00 , H01J49/40 , G01N31/00 , G01N33/00 , G01N23/00 , G21K7/00
CPC分类号： H01J49/0418
摘要： A sample is sliced obliquely with a blade provided with a metal thin film on the surface, and the slice of the sample adhering to the blade surface is subjected to mass spectrometry. As a result, the section of the sliced sample can be analyzed immediately by mass spectrometry with high sensitivity.
摘要翻译：将样品用表面上设置有金属薄膜的刀片倾斜切片，并且将附着在刀片表面上的样品切片进行质谱分析。因此，可以通过高灵敏度的质谱立即分析切片样品的部分。

4. 发明授权

US09086404B2 Method for treating living samples and analyzing the same 有权
标题翻译：处理活体样本并进行分析的方法
公开(公告)号：US09086404B2
公开(公告)日：2015-07-21
申请号：US11867276
申请日：2007-10-04
申请人： Manabu Komatsu , Hiroyuki Hashimoto , Kazuhiro Ban , Yohei Murayama
发明人： Manabu Komatsu , Hiroyuki Hashimoto , Kazuhiro Ban , Yohei Murayama
IPC分类号： G01N33/50 , G01N35/10
CPC分类号： G01N33/5082 , G01N35/1002 , G01N2035/1041
摘要： A method is provided by which a reagent is provided to a living sample, including contacting a thin-film containing a porous body with a living sample containing cells or tissues, and discharging a solution containing at least one or more reagents to a non-contact side of the thin-film by ink-jet to provide the solution to the specific area of the living sample through pores on the thin-film, wherein the smaller diameter of the pores on the porous body relative to the diameter of cells in the living sample enables the reagent to be provided to each individual cell separately. According to this method, the reagent and the reaction product can be fixed while maintaining positioning information of the target substance in the living sample, as a pretreatment for analyzing with high positioning accuracy the target substance in each individual cell in the living sample.
摘要翻译：提供了一种将试剂提供给活体样品的方法，包括使含有多孔体的薄膜与含有细胞或组织的活体样品接触，并将含有至少一种或多种试剂的溶液排出至非接触通过喷墨使薄膜的一侧通过薄膜上的孔提供到活体样品的特定区域的溶液，其中多孔体上的孔的直径相对于活体中细胞的直径较小样品使得可以分别向每个单独的细胞提供试剂。根据该方法，可以在维持活体样品中的目标物质的定位信息的同时固定试剂和反应产物，作为用于以高定位精度分析活体样品中各个细胞中的目标物质的预处理。

5. 发明授权

US07851749B2 Method for obtaining information and device therefor 有权
标题翻译：获取信息的方法及其设备
公开(公告)号：US07851749B2
公开(公告)日：2010-12-14
申请号：US12017584
申请日：2008-01-22
申请人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
发明人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
IPC分类号： H01J49/00
CPC分类号： H01J49/142
摘要： A method for obtaining information on a mass of an object by time-of-flight mass spectrometry. This method includes placing colloidal metal particles for promoting ionization of the object inside the object at a depth ranging from 0.1 nm to 100 nm in opposition to a primary beam for the ionization; irradiating the object with the primary beam selected from the group of ions, neutral particles, and electrons, which can be focused, pulsed, and are capable of scanning, and laser beams, which can be focused, pulsed, and are capable of scanning to ionize a constituent of the object and to allow the ionized constituent to fly out of the object; and obtaining information on the mass of the flying constituent of the object by time-of-flight mass spectrometry.
摘要翻译：一种通过飞行时间质谱法获得物体质量信息的方法。该方法包括将胶体金属颗粒放置在与离子化的主光束相反的0.1nm至100nm范围内的物体内部物体的离子化; 用可以被聚焦，脉冲和能够扫描的离子，中性粒子和电子组中选择的主要束照射物体，以及可以被聚焦，脉冲且能够扫描的激光束电离物体的成分并使离子化成分从物体飞出; 并通过飞行时间质谱法获得关于物体飞行成分质量的信息。

6. 发明授权

US07795579B2 Information obtaining method 有权
标题翻译：信息获取方法
公开(公告)号：US07795579B2
公开(公告)日：2010-09-14
申请号：US12120512
申请日：2008-05-14
申请人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
发明人： Manabu Komatsu , Hiroyuki Hashimoto , Yohei Murayama , Kazuhiro Ban
IPC分类号： B01D59/44
CPC分类号： H01J49/145
摘要： An information obtaining method for obtaining information about a mass of a component of an analyte using a time of flight mass spectrometer and obtaining information about a distribution state of the component based on the obtained information about the mass includes the step of: (1) adding a self-reactive substance to the analyte on a base to facilitate ionization of the component; (2) irradiating the analyte with a primary beam in the presence of the self-reactive substance, thereby ionizing the components and allowing resulting ions to fly; (3) obtaining information about mass of the flying ions using the time of flight mass spectrometer; and (4) obtaining information about the distribution state of the component on the base based on the information about the mass.
摘要翻译：使用飞行时间质谱仪获取关于分析物的成分的质量的信息的信息获取方法，基于获得的关于质量的信息获得关于部件的分布状态的信息，包括以下步骤：（1）添加对基底上的分析物的自反应物质，以促进组分的离子化; （2）在存在自反应物质的情况下用主光束照射分析物，从而电离成分并允许所得离子飞行; （3）使用飞行时间质谱仪获取关于飞行离子质量的信息; 和（4）基于关于质量的信息获得关于组件在基础上的分布状态的信息。

7. 发明申请

US20080278706A1 METHOD FOR PRE-TREATING SPECIMEN AND METHOD FOR ANALYZING SPECIMEN 失效
标题翻译：预处理样本的方法和分析样本的方法
公开(公告)号：US20080278706A1
公开(公告)日：2008-11-13
申请号：US12113795
申请日：2008-05-01
申请人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu , Kazuhiro Ban
发明人： Yohei Murayama , Hiroyuki Hashimoto , Manabu Komatsu , Kazuhiro Ban
IPC分类号： G01N1/28
CPC分类号： G01N1/30 , G01N1/31 , G01N2001/282 , G01N2001/317
摘要： A pre-treatment method for analyzing a specified portion in a specimen composed of a biological tissue includes the steps of preparing a specimen to be analyzed, determining a specified portion in the specimen; and applying an analysis inhibitor to a portion except the specified portion of the specimen using a droplet spray method.
摘要翻译：用于分析由生物组织构成的试样中的指定部位的预处理方法包括准备待分析试样的步骤，确定试样的规定部位; 并使用液滴喷雾法将分析抑制剂施加到除了特定部分的部分之外的部分。

8. 发明申请

US20080073509A1 HIGH-SENSITIVITY MASS SPECTROMETER AND METHOD 失效
标题翻译：高灵敏度质谱仪和方法
公开(公告)号：US20080073509A1
公开(公告)日：2008-03-27
申请号：US11682500
申请日：2007-03-06
申请人： Kazuhiro Ban , Hiroyuki Hashimoto , Manabu Komatsu , Norihiko Utsunomiya , Yohei Murayama
发明人： Kazuhiro Ban , Hiroyuki Hashimoto , Manabu Komatsu , Norihiko Utsunomiya , Yohei Murayama
IPC分类号： B01D59/44
CPC分类号： H01J49/0418
摘要： A sample is sliced obliquely with a blade provided with a metal thin film on the surface, and the slice of the sample adhering to the blade surface is subjected to mass spectrometry. As a result, the section of the sliced sample can be analyzed immediately by mass spectrometry with high sensitivity.
摘要翻译：将样品用表面上设置有金属薄膜的刀片倾斜切片，并且将附着在刀片表面上的样品切片进行质谱分析。因此，可以通过高灵敏度的质谱立即分析切片样品的部分。

9. 发明申请

US20070105087A1 Biological tissue processing substrate, processing apparatus, processing method and processing kit 有权
标题翻译：生物组织加工基材，加工设备，加工方法和加工工具
公开(公告)号：US20070105087A1
公开(公告)日：2007-05-10
申请号：US11592313
申请日：2006-11-03
申请人： Kazuhiro Ban , Miki Ogawa , Norihiko Utsunomiya , Hiroyuki Hashimoto , Manabu Komatsu , Yohei Murayama
发明人： Kazuhiro Ban , Miki Ogawa , Norihiko Utsunomiya , Hiroyuki Hashimoto , Manabu Komatsu , Yohei Murayama
IPC分类号： C12Q1/00 , C12M3/00
CPC分类号： G01N33/6848 , G01N33/5082
摘要： A biological tissue processing substrate for fixing proteins in a biological tissue or degradation products of the proteins, the substrate comprising: a porous body that forms a contact surface with the biological tissue, the porous body holding in pores an enzyme for obtaining the proteins or the degradation products of the proteins from the biological tissue, wherein the proteins or the degradation products obtained by the action of the enzyme are brought into contact with a member consisting of a metal.
摘要翻译：一种用于将蛋白质固定在生物组织中的生物组织处理底物或蛋白质的降解产物，所述底物包括：与生物组织形成接触表面的多孔体，多孔体在孔中保留用于获得蛋白质的酶或使来自生物组织的蛋白质的降解产物与由金属构成的构件接触，其中通过酶的作用获得的蛋白质或降解产物与金属接触。

10. 发明授权

US08962302B2 Biological tissue processing substrate for fixing proteins or protein degradation products in tissue for analysis 有权
标题翻译：用于将蛋白质或蛋白质降解产物固定在组织中用于分析的生物组织处理底物
公开(公告)号：US08962302B2
公开(公告)日：2015-02-24
申请号：US11592313
申请日：2006-11-03
申请人： Kazuhiro Ban , Miki Ogawa , Norihiko Utsunomiya , Hiroyuki Hashimoto , Manabu Komatsu , Yohei Murayama
发明人： Kazuhiro Ban , Miki Ogawa , Norihiko Utsunomiya , Hiroyuki Hashimoto , Manabu Komatsu , Yohei Murayama
IPC分类号： C12M1/00 , C12M1/34 , C12Q1/00 , C12Q1/34 , C12Q1/37 , C12Q1/02 , C12N11/14 , C12N11/02 , C12N11/04 , G01N33/68 , G01N33/50
CPC分类号： G01N33/6848 , G01N33/5082
摘要： A biological tissue processing substrate for fixing proteins in a biological tissue or degradation products of the proteins, the substrate comprising: a porous body that forms a contact surface with the biological tissue, the porous body holding in pores an enzyme for obtaining the proteins or the degradation products of the proteins from the biological tissue, wherein the proteins or the degradation products obtained by the action of the enzyme are brought into contact with a member consisting of a metal.
摘要翻译：一种用于将蛋白质固定在生物组织中的生物组织处理底物或蛋白质的降解产物，所述底物包括：与生物组织形成接触表面的多孔体，多孔体在孔中保留用于获得蛋白质的酶或使来自生物组织的蛋白质的降解产物与由金属构成的构件接触，其中通过酶的作用获得的蛋白质或降解产物与金属接触。

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