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    • 2. 发明授权
    • Networked RF Tag for tracking people by means of loyalty cards
    • US07675422B2
    • 2010-03-09
    • US11677037
    • 2007-02-20
    • John K StevensM Jason AugustPaul WaterhouseKenneth TruongChristopher W VergeMichael J Vandenberg
    • John K StevensM Jason AugustPaul WaterhouseKenneth TruongChristopher W VergeMichael J Vandenberg
    • G08B21/22G08B21/18
    • A01K11/008A01K11/004A01K15/021G06Q10/08G06Q10/087G06Q50/02G06Q50/30G07C9/00111
    • The invention disclosed provides a method, system, and associated tag for detection and tracking of inanimate and animate objects. The novel method broadly comprises the steps of: a) attaching a low radio frequency detection tag to each of the objects, each tag comprising a tag antenna operable at a low radio frequency not exceeding 1 megahertz (preferably not exceeding 300 kilohertz), a transceiver operatively connected to the tag's antenna, the transceiver being operable to transmit and receive data signals at the low radio frequency, a data storage device operable to store data comprising identification data for identifying said detection tag, a programmed data processor operable to process data received from the transceiver and the data storage device and to send data to cause the transceiver to emit an identification signal based upon the identification data stored in said data storage device, and an energy source for activating the tag's transceiver and data processor; b) storing, in the data storage device of each tag, shipping data selected from object description data, address-of-origin data, destination address data, object vulnerability data, and object status data; c) commingling the objects in a repository selected from a warehouse and a truck, the repository being provided with at least one large loop field antenna operable at said low radio frequency; the field antenna being disposed at a distance from each object that permits effective communication therewith at the low radio frequency, d) reading the identification data and shipping data from the transceiver of each tag by interrogating all tags commingled in said repository with data signals, such as specific IP addresses or other identification codes, via said field antenna; and e) transmitting the identification data and shipping data from each tag to a central data processor to provide a tally of the objects in said repository.
    • 6. 发明授权
    • Nucleic acid sequencing with simultaneous quantitation
    • 同时进行核酸测序
    • US07655443B1
    • 2010-02-02
    • US09565249
    • 2000-05-05
    • Thomas D YagerM Jason August
    • Thomas D YagerM Jason August
    • C12P19/34C07H21/04
    • C12Q1/6851C12Q1/6869C12Q2545/101C12Q2535/101
    • Simultaneous sequencing and quantitation of a nucleic acid analyte in a sample using the same reagents for both assays is achieved by processing a sample containing, or suspected of containing the nucleic acid analyte of interest using a single set of reagents through a plurality of thermocycles to obtain a mixture of labeled polynucleotides which are used for the determination of both sequence information about the target nucleic acid and the amount of target nucleic acid present in the sample. The fragments are separated on the basis of size, for example by electrophoresis, and the label associated with the separated fragments is detected. The positions of the separated nucleic acid fragments are evaluated to obtain information about the sequence of the target nucleic acid analyte, and the intensity of a signal derived from the label associated with one or more of the separated fragments is evaluated to determine the quantity of the target nucleic acid analyte in the sample. Only one label is needed for both sequencing and quantitation, although two or more labels may be used if bidirectional sequencing is concurrently performed.
    • 使用相同试剂同时测定和定量样品中的两种测定是通过使用单个试剂通过多个热循环来处理含有或怀疑含有目的核酸分析物的样品的样品来获得的, 标记的多核苷酸的混合物,其用于确定关于靶核酸的序列信息和样品中存在的靶核酸的量。 基于大小分离片段,例如通过电泳分离,并且检测与分离的片段相关的标记。 评估分离的核酸片段的位置以获得关于靶核酸分析物的序列的信息,并且评估从与一个或多个分离的片段相关联的标签得到的信号的强度,以确定 目标核酸分析物在样品中。 排序和定量都只需要一个标签,尽管如果同时执行双向排序,则可能会使用两个或多个标签。