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1. 发明申请

US20050148072A1 Human liver progenitors 审中-公开
标题翻译：人肝脏祖细胞
公开(公告)号：US20050148072A1
公开(公告)日：2005-07-07
申请号：US10944919
申请日：2004-09-21
申请人： Lola Reid , Nicholas Moss , Hiroshi Kubota
发明人： Lola Reid , Nicholas Moss , Hiroshi Kubota
IPC分类号： G01N33/53 , A61K35/12 , A61K35/407 , A61K48/00 , A61P1/16 , A61P7/00 , A61P35/00 , C12N5/00 , C12N5/074 , C12N15/09 , C12Q1/02 , C12Q1/68 , C12N5/08
CPC分类号： C12N5/0672 , A61K35/12 , A61K2039/55594 , C12N2500/20 , C12N2500/22 , C12N2500/25 , C12N2500/36 , C12N2503/02
摘要： Methods of isolating and cryopreserving progenitors from human liver are disclosed which include processing human liver tissue to provide a substantially single cell suspension comprising progenitors and non-progenitors of one or more cell lineages found in human liver; subjecting the suspension to a debulking step, which reduces substantially the number of non-progenitors in the suspension, and which provides a debulked suspension enriched in progenitors exhibiting one or more markers associated with at least one of the one or more cell lineages; and selecting from said debulked suspension those cells, which themselves, their progeny, or more mature forms thereof express one or more markers associated with at least one of the one or more cell lineages. Among these markers are CD14, CD34, CD38, CD45, and ICAM. Hepatic progenitors are characterized as being 6-15μ in diameter, diploid, glycophorin A−, CD45−, AFP+++, ALB+, ICAM+, and with subpopulations varying in expression of CD14+. CD34++, CD38++, CD117+. These progenitor subpopulations have characteristics expected for cells that are particularly useful in liver cell and gene therapies and for establishing bioartificial organs.
摘要翻译：公开了从人肝脏分离和冷冻保存祖细胞的方法，其包括加工人肝组织以提供包含在人肝脏中发现的一种或多种细胞谱系的祖细胞和非祖细胞的基本上单细胞悬浮液; 使悬浮液经历减压步骤，其减少了悬浮液中的非祖细胞的数量，并且提供了富含前体细胞的减数分裂的悬浮液，其显示出与一种或多种细胞系中的至少一种相关联的一种或多种标志物; 以及从所述减粘悬浮液中选择这些细胞，其本身，其子代或更成熟的形式表达与所述一种或多种细胞谱系中的至少一种相关联的一种或多种标志物。这些标记物中有CD14，CD34，CD38，CD45和ICAM。肝祖细胞的特征在于直径为6-15μm，二倍体，血型糖蛋白，CD45 - ，AFP +++，ALB + > + ，ICAM ，以及与CD14 + + / / SUP的表达变化的亚群。 CD34 ++ ++，CD38 ++，CD117 + + / / 这些祖细胞亚群对于在肝细胞和基因治疗中特别有用的细胞以及用于建立生物人造器官的细胞具有预期的特征。

2. 发明申请

US20070238172A1 METHODS OF ISOLATING BIPOTENT HEPATIC PROGENITOR CELLS 审中-公开
标题翻译：分离BIPOTEHHEPATIC PROGENITOR细胞的方法
公开(公告)号：US20070238172A1
公开(公告)日：2007-10-11
申请号：US11757168
申请日：2007-06-01
申请人： Hiroshi Kubota , Lola Reid
发明人： Hiroshi Kubota , Lola Reid
IPC分类号： C12N5/06
CPC分类号： G01N33/5091 , C12N5/0672 , C12N2501/50 , C12N2501/58 , C12N2501/599
摘要： A method of obtaining a mixture of cells enriched in hepatic progenitors is developed which comprises methods yielding suspensions of a mixture of cell types, and selecting those cells that are classical MHC class I antigen(s) negative and ICAM-1 antigen positive. The weak or dull expression of nonclassical MHC class I antigen(s) can be used for further enrichment of hepatic progenitors. Furthermore, the progenitors can be selected to have a level of side scatter, a measure of granularity or cytoplasmic droplets, that is higher than that in non-parenchymal cells, such as hemopoietic cells, and lower than that in mature parenchymal cells, such as hepatocytes. Furthermore, the progeny of the isolated progenitors can express alpha-fetoprotein and/or albumin and/or CK19. The hepatic progenitors, so isolated, can grow clonally, that is an entire population of progeny can be derived from one cell. The clones of progenitors have a growth pattern in culture of piled-up aggregates or clusters. These methods of isolating the hepatic progenitors are applicable to any vertebrates including human. The hepatic progenitor cell population is expected to be useful for cell therapies, for bioartificial livers, for gene therapies, for vaccine development, and for myriad toxicological, pharmacological, and pharmaceutical programs and investigations.
摘要翻译：开发了获得富含肝祖细胞的细胞混合物的方法，其包括产生细胞类型混合物的悬浮液的方法，并选择那些经典的MHC I类抗原和ICAM-1抗原阳性的细胞。非典型MHC I类抗原的弱或暗的表达可用于进一步富集肝祖细胞。此外，可以选择祖细胞具有高于非实质细胞例如造血细胞的侧向散射，粒度或细胞质液滴的量级，并且低于成熟实质细胞中的祖细胞，例如肝细胞。此外，分离的祖细胞的后代可以表达甲胎蛋白和/或白蛋白和/或CK19。如此分离的肝祖细胞可以克隆生长，即可以从一个细胞中获得后代的全部群体。祖先的克隆在堆积聚集体或簇的培养中具有增长模式。这些分离肝祖细胞的方法适用于包括人在内的任何脊椎动物。预期肝祖细胞群可用于细胞疗法，生物人工肝，基因治疗，疫苗开发和无数毒理学，药理学和药物程序和调查。

3. 发明申请

US20080009057A1 HEPATIC STELLATE CELL PRECURSORS AND METHODS OF ISOLATING SAME 有权
标题翻译： HEPATIC STELLATE CELL PRECURSORS和隔离方法
公开(公告)号：US20080009057A1
公开(公告)日：2008-01-10
申请号：US11753326
申请日：2007-05-24
申请人： Hiroshi Kubota , Lola Reid
发明人： Hiroshi Kubota , Lola Reid
IPC分类号： C12N5/00
CPC分类号： C12N5/0018 , C12N5/067 , C12N5/0672 , C12N2500/00 , C12N2500/05 , C12N2500/20 , C12N2500/25 , C12N2500/32 , C12N2500/36 , C12N2500/38 , C12N2500/90 , C12N2501/00 , C12N2501/11 , C12N2501/235 , C12N2501/33 , G01N33/56966
摘要： The present invention relates to precursor cells to hepatic stellate cells, compositions comprising same and methods of isolating same. The surface antigenic profile of the precursors is MHC class Ia negative, ICAM-1+, VCAM-1+, β3-integrin+. In addition to expression of these surface markers, the cells also express the intracellular markers desmin, vimentin, smooth muscle α-actin, nestin, hepatocyte growth factor, stromal derived factor-1α and H1x homeobox transcriptional factor.
摘要翻译：本发明涉及肝星状细胞的前体细胞，包含其的组合物及其分离方法。前体的表面抗原谱是MHC Ia类阴性，ICAM-1 +，VCAM-1 +，β3-整联蛋白+。除了这些表面标志物的表达外，细胞还表达细胞内标志物结蛋白，波形蛋白，平滑肌α-肌动蛋白，巢蛋白，肝细胞生长因子，基质衍生因子-1α和H1x同源异体转录因子。

4. 发明申请

US20080131900A1 VARIANTS OF ALPHA-FETOPROTEIN CODING AND EXPRESSION SEQUENCES 有权
标题翻译： ALPHA-FETOPROTEIN编码和表达序列的变体
公开(公告)号：US20080131900A1
公开(公告)日：2008-06-05
申请号：US11963207
申请日：2007-12-21
申请人： Hiroshi Kubota , Robert Storms , Lola Reid
发明人： Hiroshi Kubota , Robert Storms , Lola Reid
IPC分类号： C12Q1/68 , C07K16/00 , C12N5/00
CPC分类号： C07K14/4715
摘要： The invention discloses the sequences of variant forms of alpha-fetoprotein transcripts that have been identified in human hemopoietic progenitors but not in differentiated mature cells. The variant forms of AFP (vAFP) cDNA sequences isolated from a multipotent hemopoietic cell line, K562, differ from the authentic AFP transcript, consisting of 15 exons, by lacking only exon 1. Instead of exon 1, vAFP transcripts use an additional one or two exons located in the 5′-untranslated region of the AFP gene. K562 expressed selectively vAFP, whereas a hepatocellular carcinoma cell line, HepG2, showed no detectable expression of vAFP. In normal adult tissues, vAFP transcripts is detected in the bone marrow, thymus and brain, but not the spleen, suggesting the expression occurs in normal hemopoietic progenitors. Moreover, CD34+Lin− hemopoietic stem/progenitor cells purified by flow cytometric sorting also express the variant transcripts.
摘要翻译：本发明公开了已经在人造血祖细胞中鉴定但不在分化的成熟细胞中的甲胎蛋白转录物的变体形式的序列。从多能造血细胞系K562分离的AFP（vAFP）cDNA序列的变体形式与仅由外显子1缺失的15个外显子组成的真正的AFP转录物不同。代替外显子1，vAFP转录物使用另外一个或位于AFP基因的5'非翻译区的两个外显子。 K562选择性表达vAFP，而肝细胞癌细胞系HepG2则显示无可检测的vAFP表达。在正常的成年组织中，在骨髓，胸腺和脑中检测到vAFP转录物，而不是脾脏，表明在正常造血祖细胞中发生表达。此外，通过流式细胞分选纯化的CD34 +造血干细胞/祖细胞也表达变体转录物。

5. 发明申请

US20070231853A1 METHODS OF ISOLATING BIPOTENT HEPATIC PROGENITOR CELLS 审中-公开
公开(公告)号：US20070231853A1
公开(公告)日：2007-10-04
申请号：US11758593
申请日：2007-06-05
申请人： Hiroshi Kubota , Lola Reid
发明人： Hiroshi Kubota , Lola Reid
IPC分类号： C12Q1/06 , C12N5/08
CPC分类号： G01N33/5091 , C12N5/0672 , C12N2501/50 , C12N2501/58 , C12N2501/599
摘要： A method of obtaining a mixture of cells enriched in hepatic progenitors is developed which comprises methods yielding suspensions of a mixture of cell types, and selecting those cells that are classical MHC class I antigen(s) negative and ICAM-1 antigen positive. The weak or dull expression of nonclassical MHC class I antigen(s) can be used for further enrichment of hepatic progenitors. Furthermore, the progenitors can be selected to have a level of side scatter, a measure of granularity or cytoplasmic droplets, that is higher than that in non-parenchymal cells, such as hemopoietic cells, and lower than that in mature parenchymal cells, such as hepatocytes. Furthermore, the progeny of the isolated progenitors can express alpha-fetoprotein and/or albumin and/or CK19. The hepatic progenitors, so isolated, can grow clonally, that is an entire population of progeny can be derived from one cell. The clones of progenitors have a growth pattern in culture of piled-up aggregates or clusters. These methods of isolating the hepatic progenitors are applicable to any vertebrates including human. The hepatic progenitor cell population is expected to be useful for cell therapies, for bioartificial livers, for gene therapies, for vaccine development, and for myriad toxicological, pharmacological, and pharmaceutical programs and investigations.

6. 发明申请

US20100197015A1 HUMAN LIVER PROGENITORS 审中-公开
标题翻译：人类生殖器
公开(公告)号：US20100197015A1
公开(公告)日：2010-08-05
申请号：US12695855
申请日：2010-01-28
申请人： Lola M. Reid , Nicholas Moss , Hiroshi Kubota
发明人： Lola M. Reid , Nicholas Moss , Hiroshi Kubota
IPC分类号： C12N5/071
CPC分类号： C12N5/0672 , A61K35/12 , A61K2039/55594 , C12N2500/20 , C12N2500/22 , C12N2500/25 , C12N2500/36 , C12N2503/02
摘要： Methods of isolating and cryopreserving progenitors from human liver are disclosed which include processing human liver tissue to provide a substantially single cell suspension comprising progenitors and non-progenitors of one or more cell lineages found in human liver; subjecting the suspension to a debulking step, which reduces substantially the number of non-progenitors in the suspension, and which provides a debulked suspension enriched in progenitors exhibiting one or more markers associated with at least one of the one or more cell lineages; and selecting from said debulked suspension those cells, which themselves, their progeny, or more mature forms thereof express one or more markers associated with at least one of the one or more cell lineages. Among these markers are CD14, CD34, CD38, CD45, and ICAM. Hepatic progenitors are characterized as being 6-15μ in diameter, diploid, glycophorin A−, CD45−, AFP+++, ALB+, ICAM+, and with subpopulations varying in expression of CD14+. CD34++, CD38++, CD117+. These progenitor subpopulations have characteristics expected for cells that are particularly useful in liver cell and gene therapies and for establishing bioartificial organs.
摘要翻译：公开了从人肝脏分离和冷冻保存祖细胞的方法，其包括加工人肝组织以提供包含在人肝脏中发现的一种或多种细胞谱系的祖细胞和非祖细胞的基本上单细胞悬浮液; 使悬浮液经历减压步骤，其减少了悬浮液中的非祖细胞的数量，并且提供了富含前体细胞的减数分裂的悬浮液，其显示出与一种或多种细胞系中的至少一种相关联的一种或多种标志物; 以及从所述减粘悬浮液中选择这些细胞，其本身，其子代或更成熟的形式表达与所述一种或多种细胞谱系中的至少一种相关联的一种或多种标志物。这些标记物中有CD14，CD34，CD38，CD45和ICAM。肝祖细胞的特征是直径为6-15μ，二倍体，血型糖蛋白A-，CD45-，AFP +++，ALB +，ICAM +，以及与CD14 +表达不同的亚群。 CD34 ++，CD38 ++，CD117 +。这些祖细胞亚群具有特别适用于肝细胞和基因疗法以及建立生物人造器官的细胞的特征。

7. 发明授权

US11828522B2 Cryogenic refrigerator and biomagnetic measurement apparatus 有权
公开(公告)号：US11828522B2
公开(公告)日：2023-11-28
申请号：US17207967
申请日：2021-03-22
申请人： Jun Kondo , Kunio Kazami , Hiroshi Kubota , Shunichi Matsumoto , Takahiro Umeno , Syoji Takami , Takuji Ito
发明人： Jun Kondo , Kunio Kazami , Hiroshi Kubota , Shunichi Matsumoto , Takahiro Umeno , Syoji Takami , Takuji Ito
IPC分类号： F25D19/00 , H01F6/04
CPC分类号： F25D19/006 , H01F6/04
摘要： A cryogenic refrigerator includes a cooling unit and a magnetic shielding unit. The cooling unit is configured to cool a refrigerant. The magnetic shielding unit covers around the cooling unit.

8. 发明授权

US08451579B2 Metalized film capacitor 有权
标题翻译：金属化电容器
公开(公告)号：US08451579B2
公开(公告)日：2013-05-28
申请号：US12992946
申请日：2009-06-30
申请人： Hiroki Takeoka , Hiroshi Kubota , Hiroshi Fujii , Yukikazu Ohchi , Yukihiro Shimasaki
发明人： Hiroki Takeoka , Hiroshi Kubota , Hiroshi Fujii , Yukikazu Ohchi , Yukihiro Shimasaki
IPC分类号： H01G2/12 , H01G2/22 , H01G4/255
CPC分类号： H01G4/012 , H01G4/015 , H01G4/18 , Y02T10/7022
摘要： A metalized film capacitor includes a first dielectric film, a first metal thin-film electrode provided on a surface of the first dielectric film, a second dielectric film provided on the first metal thin-film electrode, and a second metal thin-film electrode provided on the second dielectric film, such that the second metal thin-film electrode faces the first metal thin-film electrode across the second dielectric film. The surface of the first dielectric film has a surface energy ranging from 25 mN/m to 40 mN/m. The metalized film capacitor exhibits high heat resistance and a preferable self-healing effect.
摘要翻译：金属化薄膜电容器包括第一电介质膜，设置在第一电介质膜的表面上的第一金属薄膜电极，设置在第一金属薄膜电极上的第二电介质膜和设置在第一金属薄膜电极上的第二金属薄膜电极使得第二金属薄膜电极在第二介电膜上面对第一金属薄膜电极。第一电介质膜的表面的表面能为25mN / m〜40mN / m。金属化薄膜电容器具有高耐热性和优选的自愈效果。

9. 发明申请

US20120199097A1 LUBRICATION APPARATUS FOR FOUR-STROKE ENGINE 有权
标题翻译：四冲程发动机润滑装置
公开(公告)号：US20120199097A1
公开(公告)日：2012-08-09
申请号：US13502799
申请日：2010-06-23
申请人： Masaki Sugiyama , Toshihiro Tomita , Masaki Kurimoto , Hiroshi Kubota , Tetsuya Hasegawa
发明人： Masaki Sugiyama , Toshihiro Tomita , Masaki Kurimoto , Hiroshi Kubota , Tetsuya Hasegawa
IPC分类号： F01M1/04
CPC分类号： F16N7/32 , F01M1/04 , F01M3/04 , F01M11/02
摘要： A lubrication apparatus for a four-stroke engine (1) is provided that prevents lubrication failure from occurring around the crankshaft and reliably prevents oil from remaining in a valve operating chamber (30). The lubrication apparatus for the four-stroke engine (1) includes: an intake part (55) positioned below the level of an oil A in an oil reservoir (7) even if the oil reservoir (7) is tilted and the level of the oil A changes; an oil feeding passageway (54) that sucks up the oil A from the intake part (55) and supplies the oil A to the crank chamber (5a); and an direct passageway (47) that allows communication between a valve operating chamber (30) and a crank chamber (5a) when a negative pressure is created in the crank chamber (5a). Open end parts (47a, 54a) of the oil feeding passageway (54) and the direct passageway (47) which are open in the crank chamber (5a) are provided to open as the piston (13) moves from a position near the top dead center to the top dead center. It is possible to control the flow rate of oil supplied to the crank chamber (5a) via the oil supply passageway (54) by means of a flow rate control passageway (67) that allows communication between a supply passageway (51) to supply oil mist from the oil reservoir (7) to the valve operating chamber (30) and the oil feeding passageway (54).
摘要翻译：提供一种用于四冲程发动机（1）的润滑装置，其防止在曲轴周围发生润滑故障，并且可靠地防止油残留在阀操作室（30）中。用于四冲程发动机（1）的润滑装置包括：即使储油器（7）倾斜并且油位（7）的水平位于油藏（7）中的位于油A的高度之下的进气部分（55）油A变化; 供油通路（54），其从所述进气部（55）吸入油A，并将油A供给到所述曲柄室（5a）; 以及当在曲柄室（5a）中产生负压时允许阀操作室（30）和曲柄室（5a）之间的连通的直接通道（47）。供油通道（54）的开口端部（47a，54a）和在曲柄室（5a）中敞开的直接通道（47）设置成随着活塞（13）从靠近顶部死中心到上死点。可以通过流量控制通道（67）来控制经由供油通道（54）供应到曲柄室（5a）的油的流量，流量控制通道（67）允许供给通道（51）从油箱（7）到阀操作室（30）和供油通道（54）的雾。

10. 发明申请

US20120187870A1 LIGHTING DEVICE AND LUMINAIRE 审中-公开
标题翻译：照明设备和灯具
公开(公告)号：US20120187870A1
公开(公告)日：2012-07-26
申请号：US13354771
申请日：2012-01-20
申请人： Naoko Iwai , Hiroshi Kubota , Masahiko Kamata , Hiroshi Terasaka
发明人： Naoko Iwai , Hiroshi Kubota , Masahiko Kamata , Hiroshi Terasaka
IPC分类号： H05B37/02
CPC分类号： H05B33/0884 , H05B37/03 , H05B41/2851
摘要： According to one embodiment, a lighting device includes a lighting circuit and a control circuit. The lighting circuit includes an output end to which an illumination lamp is connected, and lights the illumination lamp by a direct-current power. The control circuit controls the lighting circuit so that power consumption caused by a contact resistance at a connection part between the illumination lamp and the output end of the lighting circuit during whole light lighting of the illumination lamp does not exceed a specified value set to 8 W or less.
摘要翻译：根据一个实施例，照明装置包括点亮电路和控制电路。照明电路包括连接照明灯的输出端，并通过直流电照明照明灯。控制电路控制照明电路，使得在照明灯的整个点亮期间，照明灯与照明电路的输出端之间的连接部分处的接触电阻引起的功率消耗不超过设定为8W的规定值或更少。

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