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1. 发明授权

US5162200A Method for determination of a peroxidate-acting substance with a diarylimidazole compound 失效
标题翻译：用二胺咪唑化合物测定过氧化物作用物质的方法
公开(公告)号：US5162200A
公开(公告)日：1992-11-10
申请号：US530661
申请日：1990-05-30
申请人： Hans-Peter Braun , Ulfert Deneke , Werner Guethlein , Rolf Nagel
发明人： Hans-Peter Braun , Ulfert Deneke , Werner Guethlein , Rolf Nagel
IPC分类号： G01N31/22 , C07D233/54 , C07D233/64 , C07D455/04 , C12Q1/26 , C12Q1/28 , C12Q1/50
CPC分类号： C07D233/64 , C12Q1/28 , C12Q1/50 , C12Q2326/00
摘要： Diarylimidazoles of the general formula: ##STR1## wherein R.sup.1 is a radical of the general formula: ##STR2## in which R.sup.3 and R.sup.6 are hydrogen atoms, R.sup.4 and R.sup.5, which can be the same or different, are lower alkyl radicals or R.sup.3 /R.sup.4 and/or R.sup.5 /R.sup.6 in each case together represent a lower alkylene radical and R.sup.2 is a lower alkyl radical, and/or of a salt thereof, are used as redox indicators in the colorimetric diarylimidazoles are not influenced by the presence of a SH group-containing substance. The peroxidate-acting substance can be used to determine the presence of creatine kinase.
摘要翻译：具有以下通式的二芳基咪唑：其中R 1是具有以下通式的基团：其中R 3和R 6是氢原子，R 4和R 5可以相同或不同，在各种情况下，低级烷基或R 3 / R 4和/或R 5 / R 6一起代表低级亚烷基，R 2为低级烷基，和/或其盐用作比色二芳基咪唑中的氧化还原指示剂不受影响通过存在含SH基团的物质。过氧化物作用物质可用于测定肌酸激酶的存在。

2. 发明授权

US5274095A Diarylimidazoles 失效
标题翻译：二芳基咪唑
公开(公告)号：US5274095A
公开(公告)日：1993-12-28
申请号：US928000
申请日：1992-08-11
申请人： Hans-Peter Braun , Ulfert Deneke , Werner Guethlein , Rolf Nagel
发明人： Hans-Peter Braun , Ulfert Deneke , Werner Guethlein , Rolf Nagel
IPC分类号： C07D233/54 , C12Q1/28 , C12Q1/50 , C07D401/04 , C07D233/64
CPC分类号： C07D233/64 , C12Q1/28 , C12Q1/50 , C12Q2326/00
摘要： Diarylimidazoles of the general formula: ##STR1## wherein R.sup.1 is a radical of the general formula: ##STR2## in which R.sup.3 and R.sup.6 are hydrogen atoms, R.sup.4 and R.sup.5, which can be the same or different, are lower alkyl radicals or R.sup.3 /R.sup.4 and /or R.sup.5 /R.sup.6 in each case together represent a lower alkylene radical and R.sup.2 is a lower alkyl radical, and/or of a salt thereof, are useful as redox indicators, particularly in the presence of a SH group-containing substance.
摘要翻译：具有以下通式的二芳基咪唑：其中R 1是具有以下通式的基团：其中R 3和R 6是氢原子，R 4和R 5可以相同或不同，在各种情况下，低级烷基或R 3 / R 4和/或R 5 / R 6一起代表低级亚烷基，R 2为低级烷基，和/或其盐可用作氧化还原指示剂，特别是在含SH组的物质。

3. 发明授权

US5374546A Process for stabilizing 1-methylhydantoinase, use and method of it for determining an analyte and agents suitable for this 失效
标题翻译：用于稳定1-甲基乙内酰脲酶的方法，其用于测定分析物的用途和方法以及适用于此的试剂
公开(公告)号：US5374546A
公开(公告)日：1994-12-20
申请号：US822176
申请日：1992-01-16
申请人： Rolf Nagel , Ulfert Deneke , Jurgen Mistele
发明人： Rolf Nagel , Ulfert Deneke , Jurgen Mistele
IPC分类号： C12N9/86 , C12N9/96 , C12Q1/26 , C12Q1/28 , C12Q1/34 , C12N9/78
CPC分类号： C12N9/86 , C12Q1/34 , G01N2333/986
摘要： The invention concerns a process for stabilizing the enzyme 1-methylhydantoinase (NMHase) which is characterized in that the NMHase is treated with divalent metal ions, a nucleoside triphosphate and a complexing agent which complexes these divalent metal ions. In addition the invention concerns the use of an enzyme stabilized in this way in a method for the determination of an analyte in which 1-methylhydantoin is converted, as well as a corresponding reagent.
摘要翻译：本发明涉及一种稳定酶1-甲基乙内酰脲酶（NMHase）的方法，其特征在于用二价金属离子，核苷三磷酸和与这些二价金属离子复合的络合剂处理NMHase。此外，本发明涉及以这种方式稳定的酶用于测定其中转化有1-甲基乙内酰脲的分析物以及相应试剂的方法。

4. 发明授权

US4876067A Multilayer test strip device with a dissolvable reagent layer 失效
标题翻译：具有可溶解试剂层的多层试片装置
公开(公告)号：US4876067A
公开(公告)日：1989-10-24
申请号：US89352
申请日：1987-08-25
申请人： Ulfert Deneke , Rolf Nagel , Anselm Rothe , Helmut Freitag
发明人： Ulfert Deneke , Rolf Nagel , Anselm Rothe , Helmut Freitag
IPC分类号： C12M1/34 , C12Q1/40 , G01N31/22 , G01N33/52
CPC分类号： G01N33/525 , Y10S435/805
摘要： The present invention provides a multi-layer test carrier (1) for the analytical determination of a component in a liquid sample, especially in a body fluid, having a first carrier layer (2) and a second carrier layer (10), at least one of which is transparent, a liquid-absorbing layer (3) being applied to the first carrier layer (2) and a reagent layer (11) containing at least one reagent being applied to the second carrier layer (10), the carrier layers being so arranged and fixed that the surfaces (3a, 11a) of the absorbing layer and of the reagent layer facing away from the carrier layer can be brought into contact with one another and can be pressed against one another, wherein the reagent layer (11) is soluble substantially without residue in the sample liquid and the take-up ability of the liquid-absorbing layer (3) for the sample liquid is so great that an amount of liquid absorbed therein suffices in order to dissolve the reagent layer free of residues so that substances contained in the reagent layer (11) pass over from the reagent layer (11) into the liquid-absorbing layer (3) when the two layers are pressed against one another.
摘要翻译：本发明提供了一种多层测试载体（1），用于分析确定具有第一载体层（2）和第二载体层（10）的液体样品中特别是体液中的组分，至少其中一个是透明的，液体吸收层（3）被施加到第一载体层（2）和试剂层（11），其中至少一个试剂被施加到第二载体层（10），载体层使得吸收层的表面（3a，11a）和试剂层背离载体层的表面（3a，11a）彼此接触并且可以彼此压靠，其中试剂层（11 ）在样品液体中基本上无残留地溶解，并且用于样品液体的液体吸收层（3）的吸收能力如此之大，使得其中吸收的液体量足以溶解没有残留物的试剂层使物质含量当所述两层被相互压靠时，所述试剂层（11）从所述试剂层（11）流入所述液体吸收层（3）。

5. 发明授权

US4603107A Reagent and process for the determination of the activity of the enzyme gamma-glutamyl transferase 失效
标题翻译：用于测定γ-谷氨酰转移酶酶活性的试剂和方法
公开(公告)号：US4603107A
公开(公告)日：1986-07-29
申请号：US532907
申请日：1983-09-16
申请人： Ulfert Deneke , Rolf Nagel , Walter Rittersdorf
发明人： Ulfert Deneke , Rolf Nagel , Walter Rittersdorf
IPC分类号： G01N33/50 , C12Q1/28 , C12Q1/48 , C12Q1/00 , C12Q1/36
CPC分类号： C12Q1/48 , Y10S435/805 , Y10S435/81
摘要： The present invention provides a process for the kinetic determination of .gamma.-glutamyl transferase activity by the reaction of a .gamma.-glutamylarylamide with the formation of an arylamine at a pH value of from 6 to 10, wherein the arylamine is simultaneously reacted with an aromatic amine or phenol appropriate as a coupling component and an appropriate oxidation agent to give a colored compound with an absorption maximum above 565 nm, the rate of formation of which is monitored photometrically and is used as a measure for the .gamma.-glutamyl transferase activity.The present invention also provides a reagent for the kinetic determination of .gamma.-GT activity, wherein it contains the following reagents:.gamma.-GT-arylamide:1-100 mmol/l..gamma.-glutamyl acceptor (glycylglycine):10-500 mmol/l.coupling component:1-500 mmol/l.oxidation agent:0.1-500 mmol/l.buffer:10-1000 mmol/l.
摘要翻译：本发明提供了一种通过γ-谷氨酰基芳基酰胺与pH6至10的芳胺形成反应来动态测定γ-谷氨酰转移酶活性的方法，其中芳胺与芳族胺同时反应或苯酚作为偶联组分和适当的氧化剂，得到最大吸收峰值高于565nm的着色化合物，其形成速率通过光度法监测并用作γ-谷氨酰转移酶活性的量度。本发明还提供用于γ-GT活性的动力学测定的试剂，其中它含有以下试剂：γ-GT-芳基酰胺：1-100mmol / l。 γ-谷氨酰受体（甘氨酰甘氨酸）：10-500mmol / l。偶联组分：1-500 mmol / l。氧化剂：0.1-500mmol / l。缓冲液：10-1000mmol / l。

6. 发明授权

US4812356A Coating composition for flexible substrates and the use thereof, and a method for the production of a protective coating 失效
标题翻译：柔性基材的涂料组合物及其用途，以及制备保护涂层的方法
公开(公告)号：US4812356A
公开(公告)日：1989-03-14
申请号：US3404
申请日：1987-03-11
申请人： Rainer-Leo Meyer , Reinmar John , Rolf Nagel , Gunter Muller
发明人： Rainer-Leo Meyer , Reinmar John , Rolf Nagel , Gunter Muller
IPC分类号： C08G18/08 , C08G18/10 , C08G18/30 , C08G18/72 , C08K5/521 , C08K13/02 , C08K13/04 , C09D175/00 , B05D3/02 , B32B27/40 , B32B9/00 , C08L75/04
CPC分类号： C08K13/04 , C08G18/0885 , C08G18/10 , C08G18/307 , C08G18/724 , C08K13/02 , C08K5/521 , C09D175/00 , C08G2150/90 , Y10T428/31551 , Y10T428/31554 , Y10T428/31565 , Y10T428/31576 , Y10T428/3158
摘要： A one-component coating composition of35 to 60 wt.-% isocyanate prepolymer8 to 15 wt.-% plasticizer0.3 to 2 wt.-% antisettling agent2 to 10 wt.-% flaky extender5 to 25 wt.-% colorant pigments1 to 3 wt.-% moisture-binding agent, and10 to 20 wt.-% aliphatic estersprovides, on a flexible substrate such as artifical leather, plastic film, paper, roofing felt, polyurethane hard and soft foams, metal foils, concrete and the like, an extraordinarily elastic, tightly adherent coating film of high covering power and light resistance. Furthermore, it has good resistance to abrasion and to weathering, even to aggressive media such as sea water and chemicals.
摘要翻译： PCT No.PCT / EP85 / 00210 Sec。 371日期1987年3月11日 102（e）1987年3月11日PCT PCT。1985年5月8日PCT公布。公开号WO86 / 06737 日期为1986年11月20日。具有35至60重量％异氰酸酯预聚物的单组分涂料组合物8至15重量％增塑剂0.3至2重量％的抗沉降剂2至10重量％片状延长剂5 至25重量％的着色剂颜料1至3重量％的湿粘结剂和10至20重量％的脂肪族酯在柔性基材上提供，例如人造革，塑料膜，纸，屋顶毡，聚氨酯硬和软泡沫，金属箔，混凝土等，具有高覆盖力和耐光性的非常弹性，紧密粘合的涂膜。此外，它具有良好的耐磨性和耐候性，甚至对海水和化学品等侵蚀性介质也是如此。

7. 发明授权

US4567230A 1-Component coating composition, utilization thereof and process for manufacturing a protection coating 失效
标题翻译： 1组分涂料组合物，其用途和制备保护涂层的方法
公开(公告)号：US4567230A
公开(公告)日：1986-01-28
申请号：US611009
申请日：1984-05-14
申请人： Rainer-Leo Meyer , Reinmar John , Rolf Nagel , Gunter Muller
发明人： Rainer-Leo Meyer , Reinmar John , Rolf Nagel , Gunter Muller
IPC分类号： C08G18/08 , C08G18/32 , C08G18/64 , C09D175/04 , E04B1/66 , E04D7/00 , E04F13/02 , C08K3/08
CPC分类号： E04F13/02 , C08G18/0885 , C08G18/3215 , C08G18/6476 , C08K3/0033 , C09D175/04 , E04B1/66 , E04D7/00 , C08G2150/90
摘要： A moisture-setting single-component coating composition has a content of 15 to 70 wt.-% of polyurethane-forming isocyanate, 5 to 25 wt.-% of light-colored, liquid, aromatic hydrocarbon resin of low molecular weight containing hydroxyl groups, 0.2 to 2 wt.-% of antisettling agent, 10 to 35 wt.-% of foliated filler, 2 to 25 wt.-% of tinting pigments, 1 to 5 wt.-% of moisture-binding agent, remainder, but no less than 5 wt.-% of conventional paint solvent. This coating composition can be applied in a conventional manner to a dirt- and dust-free substrate in a thickness of at least 20 microns, and sets of itself by the access of moisture from the ambient atmosphere. The coating composition is suitable especially for the protection of roofs of all kinds, for coating concrete, for protecting reinforced concrete against corrosion, for the painting of steel roofs and/or for the sealing of flat roofs covered with roofing felt.
摘要翻译： PCT No.PCT / EP83 / 00246 Sec。 371日期1984年5月14日 102（e）日期1984年5月14日PCT提交1983年9月15日PCT公布。出版物WO84 / 01158 日期：1984年3月29日。湿度调节单组分涂料组合物的含量为聚氨酯形成异氰酸酯的15-70重量％，浅色液体芳族烃树脂为5至25重量％含有羟基的低分子量，0.2至2重量％的抗沉降剂，10至35重量％的叶酸填料，2至25重量％的着色颜料，1至5重量％的水分结合剂，余量，但不小于5重量％的常规涂料溶剂。该涂料组合物可以以常规方式施用至厚度为至少20微米的无尘和无尘的基材，并且可以通过从环境气氛中获得水分而将其自身组合。涂料组合物特别适用于保护各种屋顶，用于涂层混凝土，用于保护钢筋混凝土免受腐蚀，用于钢屋顶的涂漆和/或用于覆盖屋顶毡的平屋顶的密封。

8. 发明授权

US09540702B2 Stabilization of enzymes with stable coenzymes 有权
标题翻译：用稳定的辅酶稳定酶
公开(公告)号：US09540702B2
公开(公告)日：2017-01-10
申请号：US13400209
申请日：2012-02-20
申请人： Wolfgang Roedel , Carina Horn , Nelli Steinke , Nadine Bucci , Thomas Meier , Rainer Schmuck , Rolf Nagel , Dieter Heindl
发明人： Wolfgang Roedel , Carina Horn , Nelli Steinke , Nadine Bucci , Thomas Meier , Rainer Schmuck , Rolf Nagel , Dieter Heindl
IPC分类号： C12N9/04
CPC分类号： C12N9/96 , C12N9/0006 , C12N9/0036 , C12Q1/26 , C12Q1/32 , C12Y101/01001 , C12Y101/01002 , C12Y101/01047 , C12Y101/01049 , C12Y106/05002 , C12Y106/99
摘要： Methods for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme are disclosed. In addition, an enzyme stabilized with a stabilized coenzyme as well as the use thereof in test elements for detecting analytes are also disclosed. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.
摘要翻译：公开了通过在稳定的辅酶的存在下储存酶来稳定酶的方法。此外，还公开了用稳定的辅酶稳定的酶以及其用于检测分析物的测试元件中的用途。其他方面包括独特的组合物，方法，技术，涉及酶稳定化的系统和装置。

9. 发明申请

US20070178546A1 STABILIZED CHOLINESTERASE SUBSTRATE SOLUTION 审中-公开
标题翻译：稳定的胆碱酯底物溶液
公开(公告)号：US20070178546A1
公开(公告)日：2007-08-02
申请号：US11566788
申请日：2006-12-05
申请人： Friederike Weber , Norbert Gottschalk , Hans-Joachim Kytzia , Matthias Muecke , Rolf Nagel
发明人： Friederike Weber , Norbert Gottschalk , Hans-Joachim Kytzia , Matthias Muecke , Rolf Nagel
IPC分类号： C12Q1/34
CPC分类号： C12Q1/46
摘要： The present invention relates to the use of a polar organic solvent for the stabilization of a cholinesterase substrate solution, wherein at least one substrate is stabilized by at least one species of a polar organic solvent in a buffered solution, and to the use of said solution for determining the activity of cholinesterase in a sample. Also within the scope of the present invention is a stabilized cholinesterase substrate solution for determining the activity of a cholinesterase in a sample, wherein at least one substrate is stabilized by at least one species of a polar organic solvent. Furthermore, the invention relates to a method for determining the activity of a cholinesterase in a sample, as well as a kit for determining the activity of a cholinesterase in a sample and a kit for conducting a method for determining the activity of a cholinesterase in a sample.
摘要翻译：本发明涉及极性有机溶剂用于稳定胆碱酯酶底物溶液的用途，其中至少一种底物由缓冲溶液中的至少一种极性有机溶剂稳定，并且使用所述溶液用于确定样品中胆碱酯酶的活性。在本发明的范围内也是用于测定样品中胆碱酯酶活性的稳定化胆碱酯酶底物溶液，其中至少一种底物由至少一种极性有机溶剂稳定。此外，本发明涉及一种用于测定样品中胆碱酯酶的活性的方法，以及用于测定样品中胆碱酯酶的活性的试剂盒和用于进行确定胆碱酯酶活性的方法的试剂盒样品。

10. 发明授权

US06413733B1 Stabilized reagent and method for determining creatine kinase 有权
标题翻译：稳定试剂和测定肌酸激酶的方法
公开(公告)号：US06413733B1
公开(公告)日：2002-07-02
申请号：US09581357
申请日：2000-06-09
申请人： Rolf Nagel , Juergen Mistele , Norbert Schroeder
发明人： Rolf Nagel , Juergen Mistele , Norbert Schroeder
IPC分类号： C12Q150
CPC分类号： C12Q1/50
摘要： A stabilized reagent for the photometric enzymatic determination of creatine kinase (CK) in biological sample material by forming ATP from creatine phosphate and ADP and detecting the ATP formed, containing an organic or inorganic sulphur compound preferably in a submolar amount relative to the added CK activator. A corresponding reagent in a liquid form is stable at 2 to 8° C. for up to 12 months without significant loss of function.
摘要翻译：一种用于通过从磷酸肌酸和ADP形成ATP并检测形成的ATP而测定生物样品材料中的肌酸激酶（CK）的稳定化试剂，所述ATP含有相对于添加的CK激活剂优选为亚摩尔量的有机或无机硫化合物。液体形式的相应试剂在2至8℃下稳定长达12个月，不会明显损失功能。

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