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1. 发明授权

US06504084B1 Maize NPR1 polynucleotides and methods of use 有权
公开(公告)号：US06504084B1
公开(公告)日：2003-01-07
申请号：US09551778
申请日：2000-04-18
申请人： Edmund H. Crane, III , Douglas A. Rice , Carl R. Simmons , John T. Tossberg , Gary A. Sandahl , Lingyu Zhang
发明人： Edmund H. Crane, III , Douglas A. Rice , Carl R. Simmons , John T. Tossberg , Gary A. Sandahl , Lingyu Zhang
IPC分类号： A01H500
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8237 , C12N15/8279 , C12N15/8282
摘要： The invention provides isolated NPR1 nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering NPR1 concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Additionally, the present invention provides promoter elements capable of initiating constitutive expression in a plant. Further, the present invention provides for methods for screening putative activators of a plant resistance pathway.

2. 发明授权

US06713665B2 Maize NPR1 promoter and methods of use in plant gene expression 失效
标题翻译：玉米NPR1启动子及其在植物基因表达中的应用
公开(公告)号：US06713665B2
公开(公告)日：2004-03-30
申请号：US10047593
申请日：2002-01-15
申请人： Edmund H. Crane, III , Douglas A. Rice
发明人： Edmund H. Crane, III , Douglas A. Rice
IPC分类号： A01H500
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8237 , C12N15/8279 , C12N15/8282
摘要： The invention provides isolated NPR1 nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering NPR1 concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Additionally, the present invention provides NPR1 promoter elements capable of initiating constitutive expression in a plant expression constructs comprising them, cells, plants and seeds comprising the constructs, and methods of using them to express heterologous nucleic acids in a plant. Further, the present invention provides for methods for screening putative activators of a plant resistance pathway.
摘要翻译：本发明提供分离的NPR1核酸及其编码的蛋白质。本发明提供了与改变植物的NPR1浓度和/或组成有关的方法和组合物。本发明还提供了重组表达盒，宿主细胞和转基因植物。另外，本发明提供了能够在包含它们的植物表达构建体，包含构建体的细胞，植物和种子中启动组成型表达的NPR1启动子元件，以及使用它们在植物中表达异源核酸的方法。此外，本发明提供了筛选植物抗性途径的推定活化剂的方法。

3. 发明授权

US07538261B2 Maize promoter active in silks, stalk nodes, roots and leaf sheaths 有权
标题翻译：玉米启动子在丝绸，茎节，根和叶鞘中活跃
公开(公告)号：US07538261B2
公开(公告)日：2009-05-26
申请号：US11584866
申请日：2006-10-23
申请人： Virginia Crane , Douglas A. Rice , Jeanne Sandahl
发明人： Virginia Crane , Douglas A. Rice , Jeanne Sandahl
IPC分类号： A01H5/00 , C07H21/04 , C12N15/63 , C12N5/14 , C12N15/82
CPC分类号： C12N15/8222 , C07K14/415
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于组织优选的玉米启动子的新的核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

4. 发明授权

US07605302B2 Maize promoter active in silks, stalk nodes, roots and leaf sheaths 有权
公开(公告)号：US07605302B2
公开(公告)日：2009-10-20
申请号：US12424179
申请日：2009-04-15
申请人： Virginia Crane , Douglas A. Rice , Jeanne Sandahl
发明人： Virginia Crane , Douglas A. Rice , Jeanne Sandahl
IPC分类号： C12N15/82 , C12N15/84 , C12N15/87 , C12N15/90
CPC分类号： C12N15/8222 , C07K14/415
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

5. 发明授权

US06177611B1 Maize promoters 有权
标题翻译：玉米促进剂
公开(公告)号：US06177611B1
公开(公告)日：2001-01-23
申请号：US09257584
申请日：1999-02-25
申请人： Douglas A. Rice
发明人： Douglas A. Rice
IPC分类号： C12N504
CPC分类号： C12N9/0008 , C07K14/415 , C07K14/825 , C12N15/8216 , C12N15/8237
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for constitutive promoters isolated from maize genes encoding histone H2B, metallothionein, alpha-tubulin 3, elongation factor efla, ribosomal protein rps8, chlorophyll a/b binding protein, and glyceraldehyde-3-phosphate dehydrogenase. A method for constitutively expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the constitutive promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物是从编码组蛋白H2B，金属硫蛋白，α-微管蛋白3，延伸因子efla，核糖体蛋白rps8，叶绿素a / b结合蛋白和甘油醛-3-磷酸脱氢酶的玉米基因中分离的组成型启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中组成型表达异源核苷酸序列的方法。该方法包括将植物细胞转化为包含可操作地连接到本发明的组成型启动子之一的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

6. 发明申请

US20090229012A1 Maize Promoter Active in Silks, Stalk Nodes, Roots and Leaf Sheaths 有权
标题翻译：玉米促进剂在丝绸，茎节，根和叶鞘中活跃
公开(公告)号：US20090229012A1
公开(公告)日：2009-09-10
申请号：US12424179
申请日：2009-04-15
申请人： Virginia Crane , Douglas A. Rice , Jeanne Sandahl
发明人： Virginia Crane , Douglas A. Rice , Jeanne Sandahl
IPC分类号： C12N15/82
CPC分类号： C12N15/8222 , C07K14/415
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于组织优选的玉米启动子的新的核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

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