专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20030215899A1 Reversible oxidation of protein tyrosine phosphatases 审中-公开
标题翻译：蛋白酪氨酸磷酸酶的可逆氧化
公开(公告)号：US20030215899A1
公开(公告)日：2003-11-20
申请号：US10366547
申请日：2003-02-12
申请人： CEPTYR, Inc. , Cold Spring Harbor Laboratory
发明人： Tzu-Ching Meng , Nicholas K. Tonks , Deborah E. Cool
IPC分类号： C12Q001/42
CPC分类号： C12Q1/42
摘要： The invention relates to a method of identifying any protein tyrosine phosphatase (PTP) that undergoes reversible modification of PTP active site invariant cysteine within a cell, such that the phosphatase is transiently protected from irreversible active site invariant cysteine-directed PTP inactivating agents. Methods related to regulation of PTPs by reactive oxygen species (ROS) in a cellular environment are provided. Multiple PTPs are shown to be reversibly oxidized and inactivated following treatment of cells with H2O2 or with physiological stimuli that promote ROS formation, and inhibition of PTP function is shown to contribute to ROS-induced mitogenesis. Transient oxidation of the PTP catalytic site invariant cysteine is exploited in methods to identify which of multiple candidate PTPs are components of a given biological signal transduction pathway, without a requirement for first specifically purifying any particular candidate PTP.
摘要翻译：本发明涉及一种鉴定在细胞内进行PTP活性位点不变半胱氨酸的可逆修饰的任何蛋白质酪氨酸磷酸酶（PTP）的方法，使得磷酸酶被不可逆活性位点不变半胱氨酸指导的PTP灭活剂瞬时保护。提供了与细胞环境中活性氧（ROS）调节PTP有关的方法。在用H 2 O 2处理细胞后，或者促进ROS形成的生理刺激，多个PTP被显示为可逆的氧化和灭活，并且显示PTP功能的抑制有助于ROS诱导的有丝分裂。 PTP催化位点不变半胱氨酸的瞬时氧化被用于鉴定多种候选PTP中的哪一种是给定生物信号转导途径的组分的方法，而不需要首先特异性纯化任何特定的候选PTP。

IPRDB

热门服务

关于我们

友情链接

联系方式