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1. 发明申请

US20180127839A1 ASSAY FOR DETECTING CLOSELY-RELATED SEROTYPES OF HUMAN PAPILLOMAVIRUS (HPV) 审中-公开
公开(公告)号：US20180127839A1
公开(公告)日：2018-05-10
申请号：US15843957
申请日：2017-12-15
申请人： BECTON DICKINSON AND COMPANY
发明人： Chi Chen , Hugh J. Peck , Michael Porter , Gregory A. Richart , Ray A. McMillian
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708
摘要： A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

2. 发明授权

US11913083B2 Assay for detecting closely-related serotypes of human papillomavirus (HPV) 有权
公开(公告)号：US11913083B2
公开(公告)日：2024-02-27
申请号：US17094908
申请日：2020-11-11
申请人： BECTON DICKINSON AND COMPANY
发明人： Chi Chen , Hugh J. Peck , Michael Porter , Gregory A. Richart , Ray A. McMillian
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708
摘要： A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

3. 发明申请

US20210071272A1 ASSAY FOR DETECTING CLOSELY-RELATED SEROTYPES OF HUMAN PAPILLOMAVIRUS (HPV) 有权
公开(公告)号：US20210071272A1
公开(公告)日：2021-03-11
申请号：US17094908
申请日：2020-11-11
申请人： BECTON DICKINSON AND COMPANY
发明人： Chi Chen , Hugh J. Peck , Michael Porter , Gregory A. Richart , Ray A. Mc Millian
IPC分类号： C12Q1/70
摘要： A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

4. 发明授权

US09868997B2 Assay for detecting closely-related serotypes of human papillomavirus (HPV) 有权
公开(公告)号：US09868997B2
公开(公告)日：2018-01-16
申请号：US14179113
申请日：2014-02-12
申请人： Becton, Dickinson and Company
发明人： Chi Chen , Hugh J. Peck , Michael Porter , Gregory A. Richart , Ray A. McMillian
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708
摘要： A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

5. 发明公开

US20240229169A1 ASSAY FOR DETECTING CLOSELY-RELATED SEROTYPES OF HUMAN PAPILLOMAVIRUS (HPV) 审中-公开
公开(公告)号：US20240229169A1
公开(公告)日：2024-07-11
申请号：US18418143
申请日：2024-01-19
申请人： Becton, Dickinson and Company
发明人： Chi Chen , Hugh J. Peck , Michael Porter , Gregory A. Richart , Ray A. Mc Millian
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708
摘要： A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

6. 发明授权

US10865455B2 Assay for detecting closely-related serotypes of human papillomavirus (HPV) 有权
公开(公告)号：US10865455B2
公开(公告)日：2020-12-15
申请号：US15843957
申请日：2017-12-15
申请人： BECTON DICKINSON AND COMPANY
发明人： Chi Chen , Hugh J. Peck , Michael Porter , Gregory A. Richart , Ray A. McMillian
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708
摘要： A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

7. 发明申请

US20140256580A1 ASSAY FOR DETECTING CLOSELY-RELATED SEROTYPES OF HUMAN PAPILLOMAVIRUS (HPV) 有权
标题翻译：用于检测人类斑马鱼（HPV）的闭塞相关血清的测定
公开(公告)号：US20140256580A1
公开(公告)日：2014-09-11
申请号：US14179113
申请日：2014-02-12
申请人： BECTON, DICKINSON AND COMPANY
发明人： Chi Chen , Hugh J. Peck , Michael Porter , Gregory A. Richart , Ray A. Mc Millian
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708
摘要： A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.
摘要翻译：用于检测人乳头状瘤病毒（HPV）的多种血清型的实时Taq-Man PCR测定法，其中检测的血清型数量超过用于检测的比色通道的数量。将生物样品与三种寡核苷酸引物/探针组合，使得探针和引物与靶序列退火。每个引物/探针组对于生物的特定血清型至少是优选的。第一和第二引物/探针组相对于彼此退化。第三引物/探针组相对于第一和第二引物/探针组不退化，并鉴别第三血清型。第三引物/探针组具有在与简并引物/探针组探针的信号部分发射的波长相同或不同的波长处发射信号的信号部分。靶序列（如果存在）被扩增和检测。

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