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1. 发明申请

US20140154675A1 Flash and Glow 1,2-Dioxetanes 审中-公开
标题翻译：闪光和辉光1,2-二氧杂环丁烷
公开(公告)号：US20140154675A1
公开(公告)日：2014-06-05
申请号：US14114975
申请日：2012-04-30
申请人： Albana Mihali , Brooks Edwards , Zhixian Wang
发明人： Albana Mihali , Brooks Edwards , Zhixian Wang
IPC分类号： C12Q1/42 , G01N33/573
CPC分类号： C12Q1/42 , C07D321/00 , C07D407/12 , G01N33/573 , H01L21/02491
摘要： Compounds having chemiluminescent flash and glow properties. Also disclosed are methods using the compounds to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. Also disclosed are kits relating to these compounds.
摘要翻译：具有化学发光闪光和辉光性质的化合物。还公开了使用该化合物产生光，检测和/或定量酶，抗原和/或核酸的方法。还公开了与这些化合物有关的试剂盒。

2. 发明授权

US09067910B2 Chemiluminescent compositions, methods, assays and kits for oxidative enzymes 有权
标题翻译：用于氧化酶的化学发光组合物，方法，测定和试剂盒
公开(公告)号：US09067910B2
公开(公告)日：2015-06-30
申请号：US13254154
申请日：2010-03-01
申请人： Rouh-Rong Juo , Brooks Edwards , Melissa Gee , Zhixian Wang , Kathleen Skaare
发明人： Rouh-Rong Juo , Brooks Edwards , Melissa Gee , Zhixian Wang , Kathleen Skaare
IPC分类号： C07D305/14 , C07D493/00 , C07D321/00 , C07D493/10 , C07D493/04 , C07D407/04 , C12Q1/26
CPC分类号： C12Q1/26 , C07D305/14 , C07D321/00 , C07D323/00 , C07D407/04 , C07D409/04 , C07D493/04 , C07D493/10 , G01N2333/90245 , G01N2333/90251 , G01N2333/90258
摘要： Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron-withdrawing groups or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein R1 is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I. Kits, methods and assays are also disclosed that comprise the dioxetane compounds.
摘要翻译：描述了用于氧化酶的化学发光组合物，方法，测定和试剂盒。进一步公开的是以下形式的二氧杂环丁烷化合物：其中R可以独立地为任何为二氧杂环丁烷提供稳定化的支链烷基或环烷基，或者其中两个R基团一起形成与二氧杂环丁烷环结合的环烷基或多环烷基部分，其中每个R基团或螺结合部分可以是未取代的或被一个或多个吸电子基团或给电子基团或提供优先氧化同功酶底物识别的基团取代，并且其中R1是芳基或1-20个碳原子的烷基，其可以任选地被1个或多个卤素原子取代，并且其中T是在二氧杂环丁烷I的酶活化分解时能够发光的芳基或杂芳基环。还公开了包含二恶丁环化合物的方法和测定法。

3. 发明申请

US20060216768A1 Dioxetane-nanoparticle assemblies for energy transfer detection systems, methods of making the assemblies, and methods of using the assemblies in bioassays 审中-公开
标题翻译：用于能量转移检测系统的二氧杂环丁烷 - 纳米颗粒组件，制造组件的方法和在生物测定中使用组件的方法
公开(公告)号：US20060216768A1
公开(公告)日：2006-09-28
申请号：US11221895
申请日：2005-09-09
申请人： Alison Sparks , Zhixian Wang , Brooks Edwards , Rouh-Rong Juo
发明人： Alison Sparks , Zhixian Wang , Brooks Edwards , Rouh-Rong Juo
IPC分类号： G01N33/533
CPC分类号： G01N33/588 , B82Y15/00 , B82Y30/00 , G01N33/581
摘要： Assemblies comprising nanoparticles and chemiluminescent substrates such as dioxetanes are provided. The assemblies can be used in assays to detect the presence and/or amount of a single analyte or multiple analytes in a sample. Methods of making the assemblies are also described.
摘要翻译：提供了包含纳米颗粒和化学发光底物如二氧杂环丁烷的组件。组合物可用于测定以检测样品中单一分析物或多个分析物的存在和/或量。还描述了制造组件的方法。

4. 发明授权

US07112413B2 Heteroaryl substituted benzothiazole dioxetanes 有权
公开(公告)号：US07112413B2
公开(公告)日：2006-09-26
申请号：US10679368
申请日：2003-10-07
申请人： Brooks Edwards , Irena Bronstein , Zhixian Wang
发明人： Brooks Edwards , Irena Bronstein , Zhixian Wang
IPC分类号： G01N33/533 , G01N33/532 , G01N21/76 , C07D277/62
CPC分类号： C07D417/04 , C07D277/62 , C07D493/04 , C07F9/65512 , C07F9/65586 , C09K9/02 , G01N33/582
摘要： Chemiluminescent heteroaryl substituted benzothiazole 1,2-dioxetane compounds capable of producing light energy when decomposed are provided. These chemiluminescent compounds are represented by the general formula: The heteroaryl substituent Y can be, for example, a pyridyl group or a benzothiazolyl group. The heteroaryl substituted benzothiazole compounds are substantially stable at room temperature. Kits including the heteroaryl substituted dioxetane compounds as well as methods for using these compounds for detecting the presence of one or more analytes in a sample are also provided.

5. 发明授权

US06660529B2 Heteroaryl substituted benzothiazole dioxetanes 有权
标题翻译：杂芳基取代的苯并噻唑二氧杂环丁烷
公开(公告)号：US06660529B2
公开(公告)日：2003-12-09
申请号：US09945652
申请日：2001-09-05
申请人： Brooks Edwards , Irena Bronstein , Zhixian Wang
发明人： Brooks Edwards , Irena Bronstein , Zhixian Wang
IPC分类号： G01N2176
CPC分类号： C07D417/04 , C07D277/62 , C07D493/04 , C07F9/65512 , C07F9/65586 , C09K9/02 , G01N33/582
摘要： Chemiluminescent heteroaryl substituted benzothiazole 1,2-dioxetane compounds capable of producing light energy when decomposed are provided. These chemiluminescent compounds are represented by the general formula: The heteroaryl substituent Y can be, for example, a pyridyl group or a benzothiazolyl group. The heteroaryl substituted benzothiazole compounds are substantially stable at room temperature. Kits including the heteroaryl substituted dioxetane compounds as well as methods for using these compounds for detecting the presence of one or more analytes in a sample are also provided.
摘要翻译：提供了能够在分解时产生光能的化学发光的杂芳基取代的苯并噻唑1,2-二氧杂环丁烷化合物。这些化学发光化合物由以下通式表示：杂芳基取代基Y可以是例如吡啶基或苯并噻唑基。杂芳基取代的苯并噻唑化合物在室温下基本稳定。还提供了包括杂芳基取代的二恶丁环化合物的试剂盒以及使用这些化合物检测样品中一种或多种分析物的存在的方法。

6. 发明申请

US20120100566A1 CHEMILUMINESCENT COMPOSITIONS, METHODS, ASSAYS AND KITS FOR OXIDATIVE ENZYMES 有权
标题翻译：氧化酶的显色组合物，方法，测定和试剂
公开(公告)号：US20120100566A1
公开(公告)日：2012-04-26
申请号：US13254154
申请日：2010-03-01
申请人： Rouh-Rong Juo , Brooks Edwards , Melissa Gee , Zhixian Wang , Kathleen Skaare
发明人： Rouh-Rong Juo , Brooks Edwards , Melissa Gee , Zhixian Wang , Kathleen Skaare
IPC分类号： C12Q1/26 , C07D409/04 , C07D323/00
CPC分类号： C12Q1/26 , C07D305/14 , C07D321/00 , C07D323/00 , C07D407/04 , C07D409/04 , C07D493/04 , C07D493/10 , G01N2333/90245 , G01N2333/90251 , G01N2333/90258
摘要： Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: 0-0 ΛR R R T (i) where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron-withdrawing groups or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein Ri is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I. Kits, methods and assays are also disclosed that comprise the dioxetane compounds.
摘要翻译：描述了用于氧化酶的化学发光组合物，方法，测定和试剂盒。进一步公开的是以下形式的二氧杂环丁烷化合物：其中R可以独立地是任何分支烷基或环烷基，其为二氧杂环丁烷提供稳定性，或者其中两个R基团一起形成环烷基或多环烷基部分螺二环氧环乙烷环，其中每个R基团或螺环结合的部分可以是未取代的或被一个或多个吸电子基团或给电子基团或提供优先氧化同功酶底物识别的基团取代，并且其中R 1是芳基或烷基 1-20个碳原子的基团，其可以任选地被1个或多个卤素原子取代，并且其中T是能够在二氧杂环丁烷I的酶活化分解时发光的芳基或杂芳基环。另外还公开了方法和测定其包含二氧杂环丁烷化合物。

7. 发明申请

US20110003277A1 Dioxetane-Nanoparticle Assemblies For Energy Transfer Detection Systems, Methods Of Making The Assemblies, And Methods Of Using The Assemblies in Bioassays 审中-公开
标题翻译：用于能量转移检测系统的二氧杂环丁烷 - 纳米粒子组件，制造组件的方法以及在生物测定中使用组件的方法
公开(公告)号：US20110003277A1
公开(公告)日：2011-01-06
申请号：US12819844
申请日：2010-06-21
申请人： Alison Sparks , Zhixian Wang , Brooks Edwards , Rouh-Rong Juo
发明人： Alison Sparks , Zhixian Wang , Brooks Edwards , Rouh-Rong Juo
IPC分类号： C12Q1/00 , H01B1/22
CPC分类号： G01N33/588 , B82Y15/00 , B82Y30/00 , G01N33/581
摘要： Assemblies comprising nanoparticles and chemiluminescent substrates such as dioxetanes are provided. The assemblies can be used in assays to detect the presence and/or amount of a single analyte or multiple analytes in a sample. Methods of making the assemblies are also described.
摘要翻译：提供了包含纳米颗粒和化学发光底物如二氧杂环丁烷的组件。组合物可用于测定以检测样品中单一分析物或多个分析物的存在和/或量。还描述了制造组件的方法。

8. 发明申请

US20060024762A1 Heteroaryl substituted benzothiazole dioxetanes 审中-公开
公开(公告)号：US20060024762A1
公开(公告)日：2006-02-02
申请号：US11229576
申请日：2005-09-20
申请人： Brooks Edwards , Irena Bronstein , Zhixian Wang
发明人： Brooks Edwards , Irena Bronstein , Zhixian Wang
IPC分类号： G01N33/573
CPC分类号： G01N33/542 , C07D417/14 , C07F9/65583 , C07F9/65586 , G01N33/573
摘要： Chemiluminescent heteroaryl substituted benzothiazole 1,2-dioxetane compounds capable of producing light energy when decomposed are provided. These chemiluminescent compounds are represented by the general formula: The heteroaryl substituent Y can be, for example, a pyridyl group or a benzothiazolyl group. The heteroaryl substituted benzothiazole compounds are substantially stable at room temperature. Kits including the heteroaryl substituted dioxetane compounds as well as methods for using these compounds for detecting the presence of one or more analytes in a sample are also provided.

9. 发明申请

US20080227124A1 Solid phases optimized for chemiluminescent detection 审中-公开
公开(公告)号：US20080227124A1
公开(公告)日：2008-09-18
申请号：US12076235
申请日：2008-03-14
申请人： Brooks Edwards , Timothy G. Geiser , Steven M. Menchen , Alison L. Sparks , John C. Voyta
发明人： Brooks Edwards , Timothy G. Geiser , Steven M. Menchen , Alison L. Sparks , John C. Voyta
IPC分类号： G01N33/53 , G01N21/76 , B05D3/02 , C12Q1/00
CPC分类号： C12Q1/6823 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B50/14 , G01N33/54393 , Y10S436/805 , Y10S436/823
摘要： Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a cationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer.

10. 发明授权

US07368296B2 Solid phases optimized for chemiluminescent detection 有权
标题翻译：为化学发光检测优化的固相
公开(公告)号：US07368296B2
公开(公告)日：2008-05-06
申请号：US10046730
申请日：2002-01-17
申请人： Brooks Edwards , Timothy G. Geiser , Steven M. Menchen , Alison L. Sparks , John C. Voyta
发明人： Brooks Edwards , Timothy G. Geiser , Steven M. Menchen , Alison L. Sparks , John C. Voyta
IPC分类号： G01N33/545 , G01N33/533 , C07C211/62
CPC分类号： C12Q1/6823 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B50/14 , G01N33/54393 , Y10S436/805 , Y10S436/823
摘要： Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a cationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer.
摘要翻译：提供了化学发光测定的固体支持物。固体支持物包括共价或物理连接到支持物表面上的多个探针和结合到表面或载体的大部分中的化学发光增强部分。固体支持物可以是包含邻近阳离子微凝胶层的上探针结合层（例如，吖内酯聚合物层或多孔功能性聚酰胺层）的多层载体。吖内酯官能聚合物可以是与乙二胺交联的二甲基丙烯酰胺和乙烯基内酯的共聚物。阳离子微凝胶层可以是交联的含季铵盐的聚合物。还提供了使用固体支持物进行化学发光测定的方法和试剂盒。试剂盒包括二氧杂环丁烷底物，生物聚合物探针 - 酶复合物和固体支持物。固体支持物可以是与阳离子微凝胶层相邻的吖内酯官能聚合物层; 与阳离子微凝胶层相邻的多孔聚酰胺官能层; 或季铵化的吖内酯官能聚合物层。

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