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1. 发明授权

US5317084A Process for large-scale production of antimicrobal peptide in high purity 失效
标题翻译：大规模生产高纯度抗微生物肽的方法
公开(公告)号：US5317084A
公开(公告)日：1994-05-31
申请号：US891174
申请日：1992-05-29
申请人： Mamoru Tomita , Seiichi Shimamura , Kozo Kawase , Yasuo Fukuwatari , Mitsunori Takase , Wayne Bellamy , Tomoyuki Hagiwara , Hiroyuki Matukuma
发明人： Mamoru Tomita , Seiichi Shimamura , Kozo Kawase , Yasuo Fukuwatari , Mitsunori Takase , Wayne Bellamy , Tomoyuki Hagiwara , Hiroyuki Matukuma
IPC分类号： A61K38/00 , A61P31/04 , C07K1/14 , C07K1/18 , C07K1/20 , C07K1/24 , C07K14/00 , C07K14/47 , C07K14/79 , C12P21/06 , C12N9/08 , C07K3/22 , C07K13/00
CPC分类号： C07K14/79 , A61K38/00
摘要： Lactoferricin (Trade Mark), also called LFCIN (Trade Mark), a potent antimicrobial peptide, is produced by contacting an enzymatic hydrolysate of bovine lactoferrin or any mixture of peptides containing lactoferricin preferably with a butyl moiety-containing hydrophobic interaction chromatography medium, or alternatively with a carboxymethyl moiety-containing cation-exchange chromatography medium, rinsing the medium to remove unbound peptides, desorbing the lactoferricin solution at constant pH, and desalting the desorbed solution. The utility of the process is illustrated, for example, as follows. An enzymatic hydrolysate of bovine lactoferrin (600 g) was contacted with 3000 ml of BUTYL-TOYOPEARL 650M, the medium was rinsed with water, and then with McIlvaine (citric acid-sodium phosphate) buffer at pH 7.0., and lactoferricin was desorbed with McIlvaine buffer at pH 5.0. The desorbed material was desalted by contacting it with BUTYL-TOYOPEARL 650M, rinsing the medium with water to remove the buffer salts, and desorbing the lactoferricin with 10 mM HCl. A yield of 10.5 g of 99% purity was obtained. The product has applications as a safe and effective antimicrobial agent for use in oral hygiene products, cosmetics, clinical foods, etc.
摘要翻译：通过使牛乳铁蛋白的酶水解物或含有乳铁蛋白的任何混合物优选与含有丁基部分的疏水相互作用色谱介质接触来产生乳铁蛋白（商标），也称为LFCIN（商标），其是有效的抗微生物肽。用含有羧甲基部分的阳离子交换层析介质，漂洗培养基以除去未结合的肽，在恒定pH下解吸乳铁蛋白溶液，并脱盐解吸溶液。该过程的实用性例如如下所示。将牛乳铁蛋白（600g）的酶水解物与3000ml BUTYL-TOYOPEARL 650M接触，用水冲洗培养基，然后用pH 7.0的McIlaine（柠檬酸 - 磷酸钠）缓冲液洗涤，并用乳糖铁蛋白溶解 McIlvaine缓冲液pH 5.0。通过与BUTYL-TOYOPEARL 650M接触将脱附的物质脱盐，用水冲洗培养基以除去缓冲盐，并用10mM HCl解吸乳铁蛋白。得到10.5g 99％纯度的产率。该产品作为安全有效的抗微生物剂应用于口腔卫生用品，化妆品，临床食品等。

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