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1. 发明授权

US5989925A Antibody to and assay for peptide linked-pyridinoline as indicator of bone resorption level 失效
公开(公告)号：US5989925A
公开(公告)日：1999-11-23
申请号：US884148
申请日：1997-06-27
申请人： Judith Fitzpatrick , Regina Lenda , Zijian Zeng
发明人： Judith Fitzpatrick , Regina Lenda , Zijian Zeng
IPC分类号： C07K16/18 , G01N33/53 , G01N33/68 , G01N33/553 , C07K16/81
CPC分类号： C07K16/18 , G01N33/5308 , G01N33/68 , Y10S436/815 , Y10S530/809
摘要： A monoclonal antibody, Serex A93, recognizes peptide linked pyridinoline in Type I bone collagen fragments and can be used to quantitate bone resorption. The hybridoma producing this antibody has been deposited with the American Type Culture Collection, Rockville, Md., under accession number HB-12254. The epitope reactive with the antibody is stable to acid hydrolysis and therefore is not a linear peptide. The antibody recognizes pyridinoline in bound or conjugated form, but not in the free form found in urine. The antibody is useful in immunoassays for determining bone resorption, utilizing body fluids such as urine, saliva, blood, or sweat.

2. 发明授权

US06455047B1 Methods to improve immunogenicity of antigens and specificity of antibodies 有权
标题翻译：提高抗原免疫原性和抗体特异性的方法
公开(公告)号：US06455047B1
公开(公告)日：2002-09-24
申请号：US09156864
申请日：1998-09-18
申请人： Judith Fitzpatrick , Regina Lenda
发明人： Judith Fitzpatrick , Regina Lenda
IPC分类号： A61K39385
CPC分类号： C07K16/16 , A61K39/385 , A61K47/646 , A61K2039/6081 , A61K2039/627 , C07K16/18
摘要： A method of improving specific immune responses to small immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. High affinity antibodies to the hapten cotinine have been produced using this method. Antibodies to a glycated protein have also been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and the peptide mimic is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In a further embodiment the peptide mimic and spacer are linked to a carrier molecule.
摘要翻译：已经通过改变用于免疫的半抗原和载体之间的连接来开发提高对小免疫原（haptens）的特异性免疫应答的方法。使用该方法已经产生了对半抗原可替宁的高亲和力抗体。还开发了利用由作为靶表位的糖基化肽序列的糖基化肽模拟物构成的免疫原的糖基化蛋白质的抗体，其中构建该肽模拟物以构象地模拟天然蛋白质中的肽的构象，肽模拟物不含有带电荷基团或其他免疫显性基团，并且肽模拟物连接到等同于长度为一至三十个氨基酸之间的肽间隔物的间隔序列，其用于将肽表位定位成近似的构象其在天然蛋白质中的构象。在另一个实施方案中，肽模拟物和间隔基连接到载体分子。

3. 发明授权

US06465655B1 Antibody to and assay for peptide linked-pyridinoline as indicator of bone resorption level 有权
标题翻译：肽连接 - 吡啶啉的抗体和测定作为骨吸收水平的指标
公开(公告)号：US06465655B1
公开(公告)日：2002-10-15
申请号：US09444162
申请日：1999-11-22
申请人： Judith Fitzpatrick , Regina Lenda , Zijian Zeng
发明人： Judith Fitzpatrick , Regina Lenda , Zijian Zeng
IPC分类号： C07D21172
CPC分类号： C07K16/18 , G01N33/5308 , G01N33/68 , Y10S436/815 , Y10S530/809
摘要： A monoclonal antibody, Serex A93, recognizes peptide linked pyridinoline in Type I bone collagen fragments and can be used to quantitate bone resorption. The hybridoma producing this antibody has been deposited with the American Type Culture Collection, Rockville, Md., under accession number HB-12254. The epitope reactive with the antibody is stable to acid hydrolysis and therefore is not a linear peptide. The antibody recognizes pyridinoline in bound or conjugated form, but not in the free form found in urine. The antibody is useful in immunoassays for determining bone resorption, utilizing body fluids such as urine, saliva, blood, or sweat.
摘要翻译：单克隆抗体Serex A93识别I型骨胶原片段中的肽连接的吡啶啉，可用于定量骨吸收。产生该抗体的杂交瘤已经保藏在美国典型培养物保藏中心，Rockville，Md。，保藏号为HB-12254。与抗体反应的表位对酸水解是稳定的，因此不是线性肽。抗体识别结合或缀合形式的吡啶啉，但不能以尿液中的游离形式存在。该抗体可用于免疫测定以确定骨吸收，利用体液如尿液，唾液，血液或汗液。

4. 发明授权

US5710009A Receptor:release ligand (reland) complexes and release assays using said reland and methods and kits based thereon 失效
标题翻译：受体：释放配体（reland）复合物和释放测定，使用所述相关性和基于其的方法和试剂盒
公开(公告)号：US5710009A
公开(公告)日：1998-01-20
申请号：US493420
申请日：1995-06-22
申请人： Judith Fitzpatrick , Regina Lenda
发明人： Judith Fitzpatrick , Regina Lenda
IPC分类号： G01N33/531 , G01N33/536 , G01N33/543 , G01N33/58 , G01N33/72 , G01N33/94 , G01N33/535
CPC分类号： G01N33/721 , G01N33/536 , G01N33/58 , G01N33/94 , Y10S435/975 , Y10S436/808
摘要： Assays using receptor:reland complexes capable of releasing the reland in the presence of an analyte are described, wherein the reland does not detectably compete with analyte for binding to the receptor. The dissociation constant of the reland and the receptor is such that no appreciable release of reland occurs in the absence of analyte for the receptor. In a preferred embodiment, the association constant of the monomeric reland and the receptor is less than or equal to about 10.sup.5 M, preferably 10.sup.3 to 10.sup.5 M, most preferably 1% or less of the association constant of the analyte and receptor. In a preferred embodiment, the reland is labelled and the amount of analyte bound to the receptor is determined from the amount of labelled reland which is released.
摘要翻译：描述了使用能够在分析物存在下释放相关物的受体：相对配合物的测定法，其中所述依赖物不可检测地与分析物竞争结合受体。相关物和受体的解离常数使得在不存在受体的分析物的情况下不会发生明显的释放释放。在优选的实施方案中，单体相对和受体的缔合常数小于或等于分析物和受体的缔合常数的约105M，优选103至105M，最优选1％或更小。在一个优选的实施方案中，依赖物被标记，并且结合于受体的分析物的量由被释放的标记的依赖性的量确定。

5. 发明授权

US07303750B2 Methods to improve immunogenicity of antigens and specificity of antibodies 有权
标题翻译：提高抗原免疫原性和抗体特异性的方法
公开(公告)号：US07303750B2
公开(公告)日：2007-12-04
申请号：US10224758
申请日：2002-08-21
申请人： Judith Fitzpatrick , Regina Lenda
发明人： Judith Fitzpatrick , Regina Lenda
IPC分类号： A61K39/385
CPC分类号： C07K16/16 , A61K39/385 , A61K47/646 , A61K2039/6081 , A61K2039/627 , C07K16/18
摘要： A method of improving specific immune response to small immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. High affinity antibodies to cotinine have been produced using this method. Antibodies to a glycated protein have also been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In another embodiment, the peptide mimic and spacer are linked to a carrier molecule.
摘要翻译：通过改变用于免疫的半抗原和载体之间的连接，已经开发了提高对小免疫原（半抗原）的特异性免疫应答的方法。使用该方法制备了可替宁的高亲和力抗体。还开发了利用由作为靶表位的糖基化肽序列的糖基化肽模拟物构成的免疫原的糖基化蛋白质的抗体，其中构建该肽模拟物以构象地模拟天然蛋白质中的肽的构象，肽模拟物不含有带电荷基团或其他免疫显性基团，并且与长度为一至三十个氨基酸的肽间隔物相当的间隔序列连接，其用于将肽表位定位成近似其构象的构象天然蛋白质。在另一个实施方案中，肽模拟物和间隔基连接至载体分子。

6. 发明授权

US07147858B2 Methods to improve immunogenicity of antigens and specificity of antibodies 有权
公开(公告)号：US07147858B2
公开(公告)日：2006-12-12
申请号：US10224800
申请日：2002-08-21
申请人： Judith Fitzpatrick , Regina Lenda
发明人： Judith Fitzpatrick , Regina Lenda
IPC分类号： A61K39/00 , G01N33/66 , G01N33/68 , G01N33/72
CPC分类号： C07K16/16 , A61K39/385 , A61K47/646 , A61K2039/6081 , A61K2039/627 , C07K16/18
摘要： A method of improving specific immune responses to small immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. High affinity antibodies to the hapten cotinine have been produced using this method. Antibodies to a glycated protein have also been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and the peptide mimic is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In a further embodiment the peptide mimic and spacer are linked to a carrier molecule.

7. 发明授权

US07335516B2 Methods to improve immunogenicity of antigens and specificity of antibodies 失效
标题翻译：提高抗原免疫原性和抗体特异性的方法
公开(公告)号：US07335516B2
公开(公告)日：2008-02-26
申请号：US10224772
申请日：2002-08-21
申请人： Judith Fitzpatrick , Regina Lenda
发明人： Judith Fitzpatrick , Regina Lenda
IPC分类号： G01N33/531 , A61K38/03 , C07K17/02 , C07K16/44
CPC分类号： C07K16/16 , A61K39/385 , A61K47/646 , A61K2039/6081 , A61K2039/627 , C07K16/18
摘要： A method of improving specific immune responses to small immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. Antibodies to a glycated protein have been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and the peptide mimic is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In a further embodiment the peptide mimic and spacer are linked to a carrier molecule.
摘要翻译：已经通过改变用于免疫的半抗原和载体之间的连接来开发提高对小免疫原（haptens）的特异性免疫应答的方法。已经开发出抗糖基化蛋白质的抗体，利用由作为靶表位的糖基化肽序列的糖基化肽模拟物构成的免疫原，其中肽模拟物构建成构象地模拟天然蛋白质中肽的构象，肽模拟物不含有带电荷基团或其他免疫显性基团，并且肽模拟物连接至长度为一至三十个氨基酸的等同于肽间隔基的间隔序列，其用于将肽表位置于近似于其的构象天然蛋白质中的构象。在另一个实施方案中，肽模拟物和间隔基连接到载体分子。

8. 发明授权

US5527686A Differential binding affinities and dissociation assays based thereon 失效
标题翻译：基于此的差异结合亲和力和解离测定
公开(公告)号：US5527686A
公开(公告)日：1996-06-18
申请号：US196092
申请日：1994-02-17
申请人： Judith Fitzpatrick , Regina Lenda
发明人： Judith Fitzpatrick , Regina Lenda
IPC分类号： G01N33/53 , G01N33/58 , G01N33/72 , G01N33/94 , G01N33/535
CPC分类号： G01N33/58 , G01N33/53 , G01N33/721 , G01N33/94 , Y10S435/975 , Y10S436/815
摘要： A method for assaying for the presence of analyte in a sample based on differential binding affinity involves detecting dissociation of a complex of receptor and ligand in the presence of analyte. The receptor binds the analyte with high affinity and with the ligand with low affinity. The receptor-ligand complex may be formed in situ or may be preformed. In the presence of free analyte, the receptor releases from the receptor-ligand complex and binds free analyte. Release of the receptor-ligand complex is detectable. A kit for performing release assays to detect the presence of analyte is also provided.
摘要翻译： PCT No.PCT / US92 / 06249 Sec。 371日期1994年2月17日 102（e）日期1994年2月17日PCT提交1992年7月29日PCT公布。公开号WO93 / 03367 日期：1993年2月18日。基于差异结合亲和力测定样品中分析物的存在的方法包括检测在分析物存在下受体和配体的复合物的解离。受体以高亲和力和低亲和力的配体结合分析物。受体 - 配体复合物可以原位形成或可以预先形成。在游离分析物的存在下，受体从受体 - 配体复合物释放并结合游离的分析物。受体 - 配体复合物的释放是可检测的。还提供了用于进行释放测定以检测分析物存在的试剂盒。

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