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1. 发明授权

US06805867B1 Bovine rotavirus genes 有权
标题翻译：牛轮状病毒基因
公开(公告)号：US06805867B1
公开(公告)日：2004-10-19
申请号：US09232338
申请日：1999-01-15
申请人： Linda J. Saif , Anil Parwani , Kyeong-Ok Chang , Wonyong Kim , Kathy Gadfield
发明人： Linda J. Saif , Anil Parwani , Kyeong-Ok Chang , Wonyong Kim , Kathy Gadfield
IPC分类号： A61K3915
CPC分类号： C07K14/005 , A61K2039/525 , C12N7/00 , C12N2710/14143 , C12N2720/12322 , C12N2720/12323
摘要： The present invention provides the genes encoding the following BRV proteins: for group A, the VP4 and VP7 proteins of the Indiana (IND) strain, and the VP7 protein of the 2292B strain; for group B, the VP7 protein of the WD653 strain; for group C, the VP4 and VP7 proteins of the Shintoku strain. The genes are useful for producing nucleic acid probes which are complementary to the VP7 and VP4 genes. Such probes are useful for detecting the presence of group A,B, and C BRV in fecal samples from diarrheic calves and for determining the serotype of the BRV field isolates. The genes are also useful for producing partial length nucleic acid probes which are complementary to hypervariable regions of the VP4 and VP7 genes. The present invention also relates to partially purified VP2, VP4, VP6 and VP7 proteins of the IND strain and VP4 and VP7 of the 2292B strain, the partially purified VP7 protein of the WD653 strain, and partially purified VP2, VP4 and VP7 proteins of the Shintoku strain. The present invention also relates to recombinant virus-like particles (VLPs) which comprise one or more of the VP2, VP4, VP6, and VP7 proteins of the BRV strains IND, 2292B, CR, WD653, and Shintoku.
摘要翻译：本发明提供编码以下BRV蛋白的基因：对于组A，印第安纳（IND）菌株的VP4和VP7蛋白以及2292B菌株的VP7蛋白; 对于组B，WD653菌株的VP7蛋白; 对于C组，Shintoku菌株的VP4和VP7蛋白。该基因可用于产生与VP7和VP4基因互补的核酸探针。这样的探针可用于检测来自腹泻小牛的粪便样品中A，B和C组的BRV存在，并用于确定BRV野分离株的血清型。该基因也可用于产生与VP4和VP7基因的高变区互补的部分长度核酸探针。本发明还涉及IND菌株的部分纯化的VP2，VP4，VP6和VP7蛋白以及VP4和VP7的VP4和VP7 2292B菌株，WD653菌株的部分纯化的VP7蛋白，以及Shintoku菌株的部分纯化的VP2，VP4和VP7蛋白。本发明还涉及包含BRV株IND，2292B，CR，WD653和Shintoku的一种或多种VP2，VP4，VP6和VP7蛋白的重组病毒样颗粒（VLP）。

2. 发明授权

US4341870A Cultivatable human rotavirus type 2 失效
标题翻译：可培养人类轮状病毒2型
公开(公告)号：US4341870A
公开(公告)日：1982-07-27
申请号：US208389
申请日：1980-11-19
申请人： Richard G. Wyatt , Walter D. James , Edward H. Bohl , Kenneth W. Theil , Linda J. Saif , Anthony R. Kalica , Harry B. Greenberg , Albert Z. Kapikian , Robert M. Chanock
发明人： Richard G. Wyatt , Walter D. James , Edward H. Bohl , Kenneth W. Theil , Linda J. Saif , Anthony R. Kalica , Harry B. Greenberg , Albert Z. Kapikian , Robert M. Chanock
IPC分类号： A61K39/15 , C12N7/08 , A61K39/12
CPC分类号： C12N7/00 , A61K39/12 , A61K39/15 , C12N2720/12334 , C12N2720/12364
摘要： This invention relates to a precursor or intermediate for a rotavirus vaccine to protect infants and young children against diarrhea caused by rotavirus. The invention is the efficient propagation of a strain of human rotavirus type 2 prepared by a method of cultivation by multiple passages in vivo in gnotobiotic piglets and by multiple passages in vitro subsequently in AGMK (African green monkey kidney) cell cultures. Additionally, rotavirus is treated with a biologically active amount of trypsin prior to growth in AGMK cells, and the inoculated cell cultures are centrifuged at a low centrifugal force before incubation.
摘要翻译：本发明涉及轮状病毒疫苗的前体或中间体，用于保护婴儿和幼儿免受由轮状病毒引起的腹泻。本发明是在AGMK（非洲绿猴肾）细胞培养物中，通过在体内多次通过生物体仔猪和体外多次传代培养的方法制备的人轮状病毒2型菌株的有效繁殖。另外，轮状病毒在AGMK细胞中生长之前用生物活性量的胰蛋白酶处理，接种的细胞培养物在孵育前以低离心力离心。

3. 发明授权

US5959093A Bovine rotavirus genes 失效
标题翻译：牛轮状病毒基因
公开(公告)号：US5959093A
公开(公告)日：1999-09-28
申请号：US671978
申请日：1996-06-28
申请人： Linda J. Saif , Anil Parwani , Kyeong-Ok Chang , Wonyong Kim , Kathy Gadfield
发明人： Linda J. Saif , Anil Parwani , Kyeong-Ok Chang , Wonyong Kim , Kathy Gadfield
IPC分类号： C07K14/14 , C12N7/04 , C07H21/04
CPC分类号： C07K14/005 , C12N7/00 , A61K2039/525 , C12N2710/14143 , C12N2720/12322 , C12N2720/12323
摘要： The present invention provides the genes encoding the following BRV proteins: for group A, the VP4 and VP7 proteins of the Indiana (IND) strain, and the VP7 protein of the 2292B strain; for group B, the VP7 protein of the WD653 strain; for group C, the VP4 and VP7 proteins of the Shintoku strain. The genes are useful for producing nucleic acid probes which are complementary to the VP7 and VP4 genes. Such probes are useful for detecting the presence of group A,B, and C BRV in fecal samples from diarrheic calves and for determining the serotype of the BRV field isolates. The genes are also useful for producing partial length nucleic acid probes which are complementary to hypervariable regions of the VP4 and VP7 genes.The present invention also relates to partially purified VP2, VP4, VP6 and VP7 proteins of the IND strain and VP4 and VP7 of the 2292B strain, the partially purified VP7 protein of the WD653 strain, and partially purified VP2, VP4 and VP7 proteins of the Shintoku strain. The present invention also relates to recombinant virus-like particles (VLPs) which comprise one or more of the VP2, VP4, VP6, and VP7 proteins of the BRV strains IND, 2292B, CR, WD653, and Shintoku.
摘要翻译：本发明提供编码以下BRV蛋白的基因：对于组A，印第安纳（IND）菌株的VP4和VP7蛋白以及2292B菌株的VP7蛋白; 对于组B，WD653菌株的VP7蛋白; 对于C组，Shintoku菌株的VP4和VP7蛋白。该基因可用于产生与VP7和VP4基因互补的核酸探针。这样的探针可用于检测来自腹泻小牛的粪便样品中A，B和C组的BRV存在，并用于确定BRV野分离株的血清型。这些基因也可用于生产与VP4和VP7基因的高变区互补的部分长度核酸探针。本发明还涉及IND菌株的部分纯化的VP2，VP4，VP6和VP7蛋白，2292B菌株的VP4和VP7，WD653菌株的部分纯化的VP7蛋白，以及部分纯化的VP2，VP4和VP7蛋白新宿菌株本发明还涉及包含BRV株IND，2292B，CR，WD653和Shintoku的一种或多种VP2，VP4，VP6和VP7蛋白的重组病毒样颗粒（VLP）。

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