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1. 发明授权

US08759077B2 Apparatus for selective excitation of microparticles 有权
标题翻译：微粒选择性激发装置
公开(公告)号：US08759077B2
公开(公告)日：2014-06-24
申请号：US11846067
申请日：2007-08-28
申请人： Stanley S. Hong , Jekwan Ryu , Jong Buhm Park
发明人： Stanley S. Hong , Jekwan Ryu , Jong Buhm Park
IPC分类号： C12M1/34 , C12M3/00 , B01J19/00 , G01N33/543 , B01L7/00
CPC分类号： C12Q1/6869 , B01J19/0046 , B01L7/52 , G01N21/6452 , G01N33/54366 , G01N33/54373
摘要： Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an optical fiber delivering a first laser beam, and an interference pattern generation module coupled to the optical fiber. The interference pattern generation module splits the first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams.
摘要翻译：通过使用一种或多种试剂对微粒进行测序反应来对核酸微粒进行测序，选择性地以激发图案激发微粒，以不足以分辨单个微粒的分辨率光学成像微粒，并使用以下步骤处理微粒的光学图像关于激发模式的信息以确定光学特征的存在或不存在，其指示核酸的序列信息。用于微粒的光激发的装置包括传送第一激光束的光纤和耦合到光纤的干涉图案生成模块。干涉图案生成模块将第一激光束分割成第二和第三激光束，并产生用于通过第二和第三激光束之间的干涉选择性地激发微粒的激励图案。

2. 发明授权

US08222040B2 Nucleic acid sequencing by selective excitation of microparticles 有权
标题翻译：通过选择性激发微粒进行核酸测序
公开(公告)号：US08222040B2
公开(公告)日：2012-07-17
申请号：US11846049
申请日：2007-08-28
申请人： Stanley S. Hong , Jekwan Ryu , Jong Buhm Park
发明人： Stanley S. Hong , Jekwan Ryu , Jong Buhm Park
IPC分类号： G01N21/63 , G06K9/00 , C12Q1/68
CPC分类号： G06K9/00 , C12Q1/6869 , G01N21/6428 , G06K2209/07 , Y10T436/143333 , C12Q2563/107 , C12Q2563/149
摘要： Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more reagents, selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an optical fiber delivering a first laser beam, and an interference pattern generation module coupled to the optical fiber. The interference pattern generation module splits the first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams.
摘要翻译：通过使用一种或多种试剂对微粒进行测序反应来对核酸微粒进行测序，选择性地以激发图案激发微粒，以不足以分辨单个微粒的分辨率光学成像微粒，并使用以下步骤处理微粒的光学图像关于激发模式的信息以确定光学特征的存在或不存在，其指示核酸的序列信息。用于微粒的光激发的装置包括传送第一激光束的光纤和耦合到光纤的干涉图案生成模块。干涉图案生成模块将第一激光束分割成第二和第三激光束，并产生用于通过第二和第三激光束之间的干涉选择性地激发微粒的激励图案。

3. 发明申请

US20140323325A1 Molecular imaging and related methods 审中-公开
标题翻译：分子成像及相关方法
公开(公告)号：US20140323325A1
公开(公告)日：2014-10-30
申请号：US13999508
申请日：2014-03-04
申请人： Marc Beal , Ronald M. Cook , Peter Jay Coassin , Arturo Orjalo , Jekwan Ryu
发明人： Marc Beal , Ronald M. Cook , Peter Jay Coassin , Arturo Orjalo , Jekwan Ryu
IPC分类号： C12Q1/68 , G01N21/64
CPC分类号： C12Q1/6841 , C12Q1/6883 , C12Q1/6886 , C12Q2600/158 , G01N21/6428 , G01N21/6458 , G02B21/0004 , G02B21/0096 , G02B21/082 , G02B21/16 , G02B21/18 , C12Q2537/143 , C12Q2543/10 , C12Q2563/107 , C12Q2565/601
摘要： The present invention generally relates to imaging single molecules, or one or more collections of single molecules, and methods related to the imaging. In a method aspect, the present invention provides a method of imaging single molecules. The method comprises the steps of: a) exposing a test sample to a probe, wherein the probe comprises a first portion that specifically binds to a target molecule and a second portion that is detectable as the result of one or more chemical groups that interact with light at one or more wavelengths, wherein the probe binds to a target molecule to provide a complex; b) exposing the complex to one or more wavelengths of light that interact with the one or more chemical groups; c) detecting a result from the interacting of one or more wavelengths of light that interact with the one or more chemical groups to provide an image of one or more single molecules. The image possesses a resolution better than 450 nm over a view field area of at least 1×105 μm2, and wherein the image is obtained in a single detection step without variation of any detection settings.
摘要翻译：本发明一般涉及成像单分子，或单个分子的一个或多个集合，以及与成像有关的方法。在方法方面，本发明提供了单分子成像的方法。该方法包括以下步骤：a）将测试样品暴露于探针，其中所述探针包含特异性结合靶分子的第一部分和作为一个或多个化学基团相互作用的结果可检测的第二部分一个或多个波长的光，其中所述探针结合靶分子以提供复合物; b）将所述复合物暴露于与所述一个或多个化学基团相互作用的一个或多个波长的光; c）检测与一个或多个化学基团相互作用的一种或多种波长的光的相互作用的结果，以提供一个或多个单个分子的图像。该图像在至少1×105μm2的视野区域中的分辨率优于450nm，并且其中在单个检测步骤中获得图像而不改变任何检测设置。

4. 发明申请

US20050239114A1 Methods and devices for microarray image analysis 审中-公开
标题翻译：用于微阵列图像分析的方法和装置
公开(公告)号：US20050239114A1
公开(公告)日：2005-10-27
申请号：US11101043
申请日：2005-04-06
申请人： Jekwan Ryu , Richard Rava , Stephen Fodor
发明人： Jekwan Ryu , Richard Rava , Stephen Fodor
IPC分类号： C12Q1/68 , G01N21/64 , G01N33/48 , G01N33/50 , G06F19/00 , G06K9/00
CPC分类号： G01N21/6456 , B01J2219/00576 , B01J2219/00722 , B01J2219/00729 , G01N21/6452 , G01N2201/06113
摘要： The present invention provides methods and devices for high sensitivity and high speed microarray optical imaging. The methods include using patterned excitation to obtain a series of images and analyzing the images to resolve probe intensities which reflect the hybridization or binding between target and probes. Probe feature information and patterned excitation (structured illumination) information are incorporated into the analysis.
摘要翻译：本发明提供用于高灵敏度和高速微阵列光学成像的方法和装置。所述方法包括使用图案化激发以获得一系列图像并分析图像以分辨反映靶和探针之间的杂交或结合的探针强度。探头特征信息和图案激发（结构照明）信息被并入到分析中。

5. 发明申请

US20050239113A1 Methods and devices for microarray image 审中-公开
标题翻译：微阵列图像的方法和装置
公开(公告)号：US20050239113A1
公开(公告)日：2005-10-27
申请号：US11101019
申请日：2005-04-06
申请人： Jekwan Ryu , Richard Rava , Stephen Fodor
发明人： Jekwan Ryu , Richard Rava , Stephen Fodor
IPC分类号： C12Q1/68 , G01N21/64 , G01N33/48 , G01N33/50 , G06F19/00 , G06K9/00
CPC分类号： G01N21/6456 , B01J2219/00576 , B01J2219/00722 , B01J2219/00729 , G01N21/6452 , G01N2201/06113
摘要： The present invention provides methods and devices for high sensitivity and high speed microarray optical imaging. The methods include using patterned excitation to obtain a series of images and analyzing the images to resolve probe intensities which reflect the hybridization or binding between target and probes. Probe feature information and patterned excitation (structured illumination) information are incorporated into the analysis.
摘要翻译：本发明提供用于高灵敏度和高速微阵列光学成像的方法和装置。所述方法包括使用图案化激发以获得一系列图像并分析图像以分辨反映靶和探针之间的杂交或结合的探针强度。探头特征信息和图案激发（结构照明）信息被并入到分析中。

6. 发明申请

US20090061526A1 NUCLEIC ACID SEQUENCING BY SELECTIVE EXCITATION OF MICROPARTICLES 有权
标题翻译：通过选择性激活微阵列进行核酸序列测序
公开(公告)号：US20090061526A1
公开(公告)日：2009-03-05
申请号：US11846049
申请日：2007-08-28
申请人： Stanley S. Hong , Jekwan Ryu , Jong Buhm Park
发明人： Stanley S. Hong , Jekwan Ryu , Jong Buhm Park
IPC分类号： G01N33/00 , G06K9/74
CPC分类号： G06K9/00 , C12Q1/6869 , G01N21/6428 , G06K2209/07 , Y10T436/143333 , C12Q2563/107 , C12Q2563/149
摘要： Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more reagents, selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an optical fiber delivering a first laser beam, and an interference pattern generation module coupled to the optical fiber. The interference pattern generation module splits the first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams.
摘要翻译：通过使用一种或多种试剂对微粒进行测序反应来对核酸微粒进行测序，选择性地以激发图案激发微粒，以不足以分辨单个微粒的分辨率光学成像微粒，并使用以下步骤处理微粒的光学图像关于激发模式的信息以确定光学特征的存在或不存在，其指示核酸的序列信息。用于微粒的光激发的装置包括传送第一激光束的光纤和耦合到光纤的干涉图案生成模块。干涉图案生成模块将第一激光束分割成第二和第三激光束，并产生用于通过第二和第三激光束之间的干涉选择性地激发微粒的激励图案。

7. 发明申请

US20090061505A1 APPARATUS FOR SELECTIVE EXCITATION OF MICROPARTICLES 有权
标题翻译：选择性激活微波炉的装置
公开(公告)号：US20090061505A1
公开(公告)日：2009-03-05
申请号：US11846067
申请日：2007-08-28
申请人： Stanley S. Hong , Jekwan Ryu , Jong Buhm Park
发明人： Stanley S. Hong , Jekwan Ryu , Jong Buhm Park
IPC分类号： C12M1/00
CPC分类号： C12Q1/6869 , B01J19/0046 , B01L7/52 , G01N21/6452 , G01N33/54366 , G01N33/54373
摘要： Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an optical fiber delivering a first laser beam, and an interference pattern generation module coupled to the optical fiber. The interference pattern generation module splits the first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams.
摘要翻译：通过使用一种或多种试剂对微粒进行测序反应来对核酸微粒进行测序，选择性地以激发图案激发微粒，以不足以分辨单个微粒的分辨率光学成像微粒，并使用以下步骤处理微粒的光学图像关于激发模式的信息以确定光学特征的存在或不存在，其指示核酸的序列信息。用于微粒的光激发的装置包括传送第一激光束的光纤和耦合到光纤的干涉图案生成模块。干涉图案生成模块将第一激光束分割成第二和第三激光束，并产生用于通过第二和第三激光束之间的干涉选择性地激发微粒的激励图案。

8. 发明申请

US20050239115A1 Methods and devices for microarray image 审中-公开
公开(公告)号：US20050239115A1
公开(公告)日：2005-10-27
申请号：US11101087
申请日：2005-04-06
申请人： Jekwan Ryu , Richard Rava , Stephen Fodor
发明人： Jekwan Ryu , Richard Rava , Stephen Fodor
IPC分类号： C12Q1/68 , G01N21/64 , G01N33/48 , G01N33/50 , G06F19/00 , G06K9/00
CPC分类号： G01N21/6456 , B01J2219/00576 , B01J2219/00722 , B01J2219/00729 , G01N21/6452 , G01N2201/06113
摘要： The present invention provides methods and devices for high sensitivity and high speed microarray optical imaging. The methods include using patterned excitation to obtain a series of images and analyzing the images to resolve probe intensities which reflect the hybridization or binding between target and probes. Probe feature information and patterned excitation (structured illumination) information are incorporated into the analysis.

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