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    • 1. 发明授权
    • Method and/or apparatus of oligonucleotide design and/or nucleic acid detection
    • 寡核苷酸设计和/或核酸检测的方法和/或装置
    • US08234079B2
    • 2012-07-31
    • US11990290
    • 2006-08-08
    • Christopher Wing Cheong WongWing-Kin SungCharlie LeeLance David Miller
    • Christopher Wing Cheong WongWing-Kin SungCharlie LeeLance David Miller
    • G06F19/00G06F15/00G11C17/00C12N15/11
    • C12Q1/6813G06F19/20
    • It is provided a method of designing at least one oligonucleotide for nucleic acid detection comprising the following steps in any order: (I) identifying and/or selecting region(s) of at least one target nucleic acid to be amplified, the region(s) having an efficiency of amplification (AE) higher than the average AE; and (II) designing at least one oligonucleotide capable of hybridizing to the selected region(s). It is also provided a method of detecting at least one target nucleic acid comprising the steps of: (i) providing at least one biological sample; (ii) amplifying nucleic acid(s) comprised in the biological sample; (iii) providing at least one oligonucleotide capable of hybridizing to at least one target nucleic acid, if present in the biological sample; and (iv) contacting the oligonucleotide(s) with the amplified nucleic acids and detecting the oligonucleotide(s) hybridized to the target nucleic acid(s). In particular, the method is for detecting the presence of at least one pathogen, for example a virus, in at least one human biological sample. The probes may be placed on a support, for example a microarray.
    • 提供了设计用于核酸检测的至少一种寡核苷酸的方法,其包括以下步骤:(I)鉴定和/或选择待扩增的至少一种靶核酸的区域,所述区域 )具有高于平均AE的放大效率(AE); 和(II)设计至少一种能够与所选择的区域杂交的寡核苷酸。 还提供了检测至少一种靶核酸的方法,包括以下步骤:(i)提供至少一种生物样品; (ii)扩增生物样品中包含的核酸; (iii)提供至少一种能够与至少一种靶核酸杂交的寡核苷酸,如果存在于生物样品中; 和(iv)使寡核苷酸与扩增的核酸接触并检测与靶核酸杂交的寡核苷酸。 特别地,该方法用于在至少一种人类生物样品中检测至少一种病原体,例如病毒的存在。 探针可以放置在载体上,例如微阵列。
    • 2. 发明申请
    • Method of probe design and/or of nucleic acids detection
    • 探针设计方法和/或核酸检测方法
    • US20070042388A1
    • 2007-02-22
    • US11202023
    • 2005-08-12
    • Christopher WongWing-Kin SungCharlie LeeLance Miller
    • Christopher WongWing-Kin SungCharlie LeeLance Miller
    • C12Q1/68G06F19/00
    • C12Q1/6813G16B25/00
    • It is provided a method of designing oligonucleotide probe(s) for nucleic acid detection comprising the following steps in any order: (i) identifying and selecting region(s) of a target nucleic acid to be amplified, the region(s) having an efficiency of amplification (AE) higher than the average AE; and (ii) designing oligonucleotide probe(s) capable of hybridizing to the selected region(s). It is also provided a method of detecting at least one target nucleic acid comprising the steps of: (i) providing a biological sample; (ii) amplifying the nucleic acid(s) of the biological sample; (iii) providing at least an oligonucleotide probe capable of hybridizing to at least a target nucleic acid, if present in the biological sample; and (iv) contacting the probe(s) with the amplified nucleic acids and detecting the probe(s) hybridized to the target nucleic acid(s). In particular, the method indicates the presence of at least a pathogen, for example a virus, in a human biological sample. The probes may be placed on a support, for example a microarray or a biochip.
    • 提供了设计用于核酸检测的寡核苷酸探针的方法,其包括以下步骤:(i)鉴定和选择待扩增的靶核酸的区域,所述区域具有 放大效率(AE)高于平均AE; 和(ii)设计能够与所选择的区域杂交的寡核苷酸探针。 还提供了检测至少一种靶核酸的方法,包括以下步骤:(i)提供生物样品; (ii)扩增生物样品的核酸; (iii)至少提供能够与生物样品中存在的至少靶核酸杂交的寡核苷酸探针; 和(iv)使探针与扩增的核酸接触并检测与靶核酸杂交的探针。 特别地,该方法表明在人类生物样品中存在至少一种病原体,例如病毒。 探针可以放置在载体上,例如微阵列或生物芯片。
    • 4. 发明授权
    • Plasma enhanced method for increasing silicon-containing photoresist selectivity
    • 用于增加含硅光刻胶选择性的等离子体增强方法
    • US06799907B2
    • 2004-10-05
    • US10346470
    • 2003-01-16
    • Francis KoSandy ChenCharlie Lee
    • Francis KoSandy ChenCharlie Lee
    • G03D500
    • H01L21/0274G03F7/40H01L21/31144
    • Provided is a method for increasing an etching selectivity of photoresist material. The method initiates with providing a substrate with a developed photoresist layer. The developed photoresist layer on the substrate is formulated to contain a hardening agent. Next, the substrate is exposed to a gas, where the gas is formulated to interact with the hardening agent. A portion of the developed photoresist layer is then converted to a hardened layer where the hardened layer is created by an interaction of the hardening agent with the gas. Some notable advantages of the discussed methods of increasing the selectivity of a photoresist include improved etch profile control. Additionally, by combining fabrication steps such as the hardening of the photoresist in an etch chamber, downstream etching processes may be performed without having to transfer the wafer to an additional chamber, thereby improving wafer throughput while minimizing handling.
    • 提供了增加光致抗蚀剂材料的蚀刻选择性的方法。 该方法开始于提供具有显影的光致抗蚀剂层的基底。 将显影的光致抗蚀剂层配制成含有硬化剂。 接下来,将基底暴露于气体,其中配制气体以与硬化剂相互作用。 然后将显影的光致抗蚀剂层的一部分转化为硬化层,其中硬化层通过硬化剂与气体的相互作用产生。 所讨论的增加光致抗蚀剂选择性的方法的一些显着优点包括改进的蚀刻轮廓控制。 另外,通过组合诸如在蚀刻室中的光致抗蚀剂的硬化的制造步骤,可以执行下游蚀刻工艺,而不必将晶片转移到附加的室,从而在最小化处理的同时提高晶片产量。
    • 7. 发明授权
    • Hardhat speakers
    • US10413011B2
    • 2019-09-17
    • US16256867
    • 2019-01-24
    • Charlie Lee Jordan
    • Charlie Lee Jordan
    • A42B3/30H04R1/02H04R1/34
    • Apparatus and associated methods relate to a wireless audio speaker module configured to play music at a volume level that will not disturb those nearby, based on adapting a wireless audio speaker to be retained within headgear, adjusting the volume of sound emitted by the speaker to a level that will not disturb those nearby, and amplifying the speaker sound as a function of the headgear interior reflecting the sound emitted by the speaker to the user's ear. In an illustrative example, an airgap may be configured between the speaker and the headgear user's ear. In various embodiments, the sound volume emitted by the speaker may be adjusted to avoid disturbing those nearby. In some examples, the headgear may be a hardhat, advantageously configured with a wireless audio speaker module to permit construction workers to listen to music amplified by reflection within their hardhats without disturbing or distracting each other.
    • 10. 发明授权
    • Plasma enhanced method for increasing silicon-containing photoresist selectivity
    • 用于增加含硅光刻胶选择性的等离子体增强方法
    • US06541361B2
    • 2003-04-01
    • US09894649
    • 2001-06-27
    • Francis KoSandy ChenCharlie Lee
    • Francis KoSandy ChenCharlie Lee
    • H01L214763
    • H01L21/0274G03F7/40H01L21/31144
    • Provided is a method for increasing an etching selectivity of photoresist material. The method initiates with providing a substrate with a developed photoresist layer. The developed photoresist layer on the substrate is formulated to contain a hardening agent. Next, the substrate is exposed to a gas, where the gas is formulated to interact with the hardening agent. A portion of the developed photoresist layer is then converted to a hardened layer where the hardened layer is created by an interaction of the hardening agent with the gas. Some notable advantages of the discussed methods of increasing the selectivity of a photoresist include improved etch profile control. Additionally, by combining fabrication steps such as the hardening of the photoresist in an etch chamber, downstream etching processes may be performed without having to transfer the wafer to an additional chamber, thereby improving wafer throughput while minimizing handling.
    • 提供了增加光致抗蚀剂材料的蚀刻选择性的方法。 该方法开始于提供具有显影的光致抗蚀剂层的基底。 将显影的光致抗蚀剂层配制成含有硬化剂。 接下来,将基底暴露于气体,其中配制气体以与硬化剂相互作用。 然后将显影的光致抗蚀剂层的一部分转化为硬化层,其中硬化层通过硬化剂与气体的相互作用产生。 所讨论的增加光致抗蚀剂选择性的方法的一些显着优点包括改进的蚀刻轮廓控制。 另外,通过组合诸如在蚀刻室中的光致抗蚀剂的硬化的制造步骤,可以执行下游蚀刻工艺,而不必将晶片转移到附加的室,从而在最小化处理的同时提高晶片产量。