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1. 发明授权

US07666640B2 DNA sequences for the expression of alloproteins 失效
标题翻译：用于表达alloproteins的DNA序列
公开(公告)号：US07666640B2
公开(公告)日：2010-02-23
申请号：US12488136
申请日：2009-06-19
申请人： Shigeyuki Yokoyama , Mikako Shirouzu , Ayako Sakamoto , Kensaku Sakamoto
发明人： Shigeyuki Yokoyama , Mikako Shirouzu , Ayako Sakamoto , Kensaku Sakamoto
IPC分类号： C12P21/06 , C12N9/00 , C07K1/00
CPC分类号： C12N9/93 , A61K48/00
摘要： The present invention discloses an expression method for non-naturally-occurring amino acid-containing protein comprising: expressing in animal cells: (A) a mutant tyrosyl-tRNA synthetase that is a mutation of tyrosyl-tRNA synthetase originating in E. coli with an enhanced specificity for a non-naturally-occurring tyrosine derivative as compared with the specificity for tyrosine; (B) suppressor tRNA originating in Bacillus species, Mycoplasma species or Staphylococcus species of eubacteria and capable of binding with the tyrosine derivative in the presence of the mutant tyrosyl tRNA synthetase; and, (C) a desired protein gene that has undergone a nonsense mutation at a desired site; wherein, the tyrosine derivative is incorporated at the location of this nonsense mutation.
摘要翻译：本发明公开了一种非天然存在的含氨基酸蛋白质的表达方法，包括：在动物细胞中表达：（A）突变型酪氨酰-tRNA合成酶，其是源自大肠杆菌的酪氨酰-tRNA合成酶的突变，其具有与酪氨酸的特异性相比，非天然存在的酪氨酸衍生物的特异性增强; （B）源自芽孢杆菌属物种，支原体属或真菌属葡萄球菌属的抑制基因tRNA，能够在突变型酪氨酰tRNA合成酶存在下与酪氨酸衍生物结合; 和（C）在期望的位点已经经历了无义突变的期望的蛋白质基因; 其中，酪氨酸衍生物被掺入该无义突变的位置。

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