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1. 发明公开

KR1020030043167A 나린지나제 효소를 이용한 진세노사이드 에프원의 제조방법 失效
标题翻译：使用NARINGINASE制备GINSENOSIDE F1的方法
公开(公告)号：KR1020030043167A
公开(公告)日：2003-06-02
申请号：KR1020010074201
申请日：2001-11-27
申请人： 주식회사 케이티앤지 , 주식회사 한국인삼공사
发明人： 고성룡 , 스즈끼유키오 , 최강주 , 조병구 , 양재원 , 김삼곤 , 김영회 , 박은경
IPC分类号： C12P33/16
摘要： PURPOSE: A process for preparing ginsenoside F1 using naringinase is provided, thereby preparing the ginsenoside F1 in higher yields. CONSTITUTION: A process for preparing ginsenoside F1 using naringinase comprises the steps of: dissolving saponin of triol family isolated from ginseng or a mixture of the saponin of triol family in a water-soluble solvent or a mixed solvent of a water-soluble solvent and an organic solvent, adding naringinase isolated from Aspergillus niger or Penicillium sp. to the mixture, and reacting them with agitation in a hot water-bath; heating the reacted mixture in hot water to terminate the reaction when the substrate is exhausted; passing the reacted solution through the column packed with ion exchange resin, passing distilled water through the column to remove saccharides and enzymes, and eluting the reacted products adsorbed to the column with ethanol; concentrating the eluate; and subjecting the concentrate to the column chromatography to obtain ginsenoside F1, wherein the saponin of triol family is ginsenoside Re, or ginsenoside Rg1; the reaction temperature is 20 to 50 deg. C; the water-soluble solvent is selected from water, acetic acid, phosphate, citrate, citrate-phosphate buffer solutions; and the organic solvent is selected from acetone, acetonitrile, dioxane, dimethyl sulfoxide, and lower alcohol.
摘要翻译：目的：提供使用柚皮苷酶制备人参皂苷F1的方法，从而以更高的产率制备人参皂甙F1。构成：使用柚皮苷酶制备人参皂苷F1的方法包括以下步骤：将从人参中分离的三醇家族的皂苷或三醇家族的皂苷的混合物溶于水溶性溶剂或水溶性溶剂和有机溶剂，加入从黑曲霉或青霉菌分离的柚皮苷酶。混合物，并在热水浴中搅拌反应; 在热水中加热反应后的混合物，以在底物排空时终止反应; 使反应溶液通过填充有离子交换树脂的柱，通过蒸馏水通过柱以除去糖和酶，并用乙醇洗脱反应的产物柱。浓缩洗脱液; 并将浓缩物进行柱色谱，得到人参皂甙F1，其中三醇族的皂苷为人参皂甙Re或人参皂甙Rg1; 反应温度为20〜50℃。 C; 水溶性溶剂选自水，乙酸，磷酸盐，柠檬酸盐，柠檬酸盐 - 磷酸盐缓冲溶液; 有机溶剂选自丙酮，乙腈，二恶烷，二甲基亚砜和低级醇。

2. 发明授权

KR100444370B1 나린지나제 효소를 이용한 진세노사이드 에프원의 제조방법 失效
标题翻译： 나린지나제효소를이용한진세노사이드에프원의제조방
公开(公告)号：KR100444370B1
公开(公告)日：2004-08-16
申请号：KR1020010074201
申请日：2001-11-27
申请人： 주식회사 케이티앤지 , 주식회사 한국인삼공사
发明人： 고성룡 , 스즈끼유키오 , 최강주 , 조병구 , 양재원 , 김삼곤 , 김영회 , 박은경
IPC分类号： C12P33/16
摘要： PURPOSE: A process for preparing ginsenoside F1 using naringinase is provided, thereby preparing the ginsenoside F1 in higher yields. CONSTITUTION: A process for preparing ginsenoside F1 using naringinase comprises the steps of: dissolving saponin of triol family isolated from ginseng or a mixture of the saponin of triol family in a water-soluble solvent or a mixed solvent of a water-soluble solvent and an organic solvent, adding naringinase isolated from Aspergillus niger or Penicillium sp. to the mixture, and reacting them with agitation in a hot water-bath; heating the reacted mixture in hot water to terminate the reaction when the substrate is exhausted; passing the reacted solution through the column packed with ion exchange resin, passing distilled water through the column to remove saccharides and enzymes, and eluting the reacted products adsorbed to the column with ethanol; concentrating the eluate; and subjecting the concentrate to the column chromatography to obtain ginsenoside F1, wherein the saponin of triol family is ginsenoside Re, or ginsenoside Rg1; the reaction temperature is 20 to 50 deg. C; the water-soluble solvent is selected from water, acetic acid, phosphate, citrate, citrate-phosphate buffer solutions; and the organic solvent is selected from acetone, acetonitrile, dioxane, dimethyl sulfoxide, and lower alcohol.
摘要翻译：目的：提供一种使用柚苷酶制备人参皂苷F1的方法，由此以较高的产率制备人参皂苷F1。构成：使用柚苷酶制备人参皂甙F1的方法包括以下步骤：将从人参分离的三醇家族的皂苷或三醇家族皂苷的混合物溶解在水溶性溶剂或水溶性溶剂和有机溶剂中，加入从黑曲霉或青霉菌分离的柚苷酶。加入到混合物中，并在搅拌下在热水浴中反应它们; 当底物耗尽时，加热反应混合物在热水中以终止反应; 使反应后的溶液通过填充有离子交换树脂的柱子，使蒸馏水通过柱子以除去糖类和酶，并用乙醇洗脱吸附到柱子上的反应产物; 浓缩洗脱液; 并将浓缩物进行柱层析得到人参皂苷F1，其中三醇家族的皂苷为人参皂苷Re或人参皂苷Rg1; 反应温度为20至50℃。 C; 水溶性溶剂选自水，乙酸，磷酸盐，柠檬酸盐，柠檬酸盐 - 磷酸盐缓冲溶液; 有机溶剂选自丙酮，乙腈，二恶烷，二甲基亚砜和低级醇。

3. 发明授权

KR100420451B1 셀룰라제 또는 락타제 조성물 와이-에이오를 이용한진세노사이드 컴파운드 케이의 제조방법 有权
标题翻译： 셀룰라제또는락타제조성물와이 - 에이오를이용용한진세노사이드컴파운드케이의제조방셀룰
公开(公告)号：KR100420451B1
公开(公告)日：2004-03-02
申请号：KR1020010074202
申请日：2001-11-27
申请人： 주식회사 케이티앤지
发明人： 고성룡 , 스즈끼유키오 , 최강주 , 조병구 , 양재원 , 박종대 , 김영회 , 박은경
IPC分类号： C12P33/16
摘要： PURPOSE: A method of preparing a ginsenoside compound K is provided to make the ginsenoside product at high yield of 80% or more with effects of immuno-stimulation, cancer inhibition, and vascular tumor inhibition. CONSTITUTION: In a method of preparing a ginsenoside compound K, a diol-based saponin extract from ginseng is dissolved in a water solvent or a mixture of a water solvent and an organic solvent, and cellulase isolated from Aspergillus niger or a lactase composition Y-AO where 78-82 wt.% of dextrin powder is mixed with 18-22 wt.% of lactase powder isolated from Aspergillus oryzae is added to the solution. The solution is stirred in a heated water bath during the enzymatic reaction. The reaction solution is mixed with water-saturated butanol to obtain a butanol layer with an eluate of ginsenoside compound K. The resulting product is concentrated, and subjected to column-chromatography to obtain ginsenoside compound K. The ginseng is selected from Panax ginseng, Panax quinquefolium, Panax japonica, or Panax notoginseng.
摘要翻译：目的：提供一种制备人参皂甙化合物K的方法，以通过免疫刺激，抑制癌症和抑制血管肿瘤的作用，以80％或更高的产率生产人参皂甙产品。构成：在制备人参皂甙化合物K的方法中，将来自人参的二醇基皂苷提取物溶于水溶剂或水溶剂和有机溶剂的混合物中，从黑曲霉或乳糖酶组合物Y- AO，其中将78-82重量％的糊精粉与18-22重量％的从米曲霉分离的乳糖酶粉末混合。在酶促反应期间将溶液在加热的水浴中搅拌。将该反应溶液与水饱和丁醇混合，得到含有人参皂苷化合物K洗脱液的丁醇层。浓缩所得产物，并进行柱层析以获得人参皂甙化合物K.人参选自人参，人参西洋参，三七或三七。

4. 发明公开

KR1020020065128A 세포 배양기 失效
标题翻译：细胞培养器
公开(公告)号：KR1020020065128A
公开(公告)日：2002-08-13
申请号：KR1020010005485
申请日：2001-02-05
申请人： 주식회사 케이티앤지
发明人： 김갑식 , 박규진 , 채순용 , 여운형 , 이미자 , 이기원 , 박은경 , 이유희 , 김영숙
IPC分类号： C12M1/14
摘要： PURPOSE: Provided is a cell cultivator which provides abundant air to the cell culture solution and prevents the generation of foam therein, thereby removing lethal factor of cultured cells. CONSTITUTION: The cultivator comprises a cultivation plate, a support of the cultivation plate, an opening bar housing the cultivation plate, an air nozzle, a fixing groove of the cultivation plate, a cultivation vessel support, a locking hook housing the cultivation vessel support and fixing a cover, an outer container having a cultivation solution outlet, an air bent, and a lid having a cultivation solution inlet and an air inlet.
摘要翻译：目的：提供一种细胞培养器，其向细胞培养溶液提供大量空气，并防止其中产生泡沫，从而去除培养细胞的致死因子。构成：耕作机包括培养板，栽培板的支撑体，容纳培养板的开放杆，空气喷嘴，培养板的固定槽，培养容器支架，容纳培养容器支架的锁定钩和固定盖子，具有培养溶液出口的外部容器，弯曲的空气和具有培养溶液入口和空气入口的盖子。

5. 发明授权

KR100394247B1 세포 배양기 失效
标题翻译： 세포배양기
公开(公告)号：KR100394247B1
公开(公告)日：2003-08-06
申请号：KR1020010005485
申请日：2001-02-05
申请人： 주식회사 케이티앤지
发明人： 김갑식 , 박규진 , 채순용 , 여운형 , 이미자 , 이기원 , 박은경 , 이유희 , 김영숙
IPC分类号： C12M1/14
摘要： PURPOSE: Provided is a cell cultivator which provides abundant air to the cell culture solution and prevents the generation of foam therein, thereby removing lethal factor of cultured cells. CONSTITUTION: The cultivator comprises a cultivation plate, a support of the cultivation plate, an opening bar housing the cultivation plate, an air nozzle, a fixing groove of the cultivation plate, a cultivation vessel support, a locking hook housing the cultivation vessel support and fixing a cover, an outer container having a cultivation solution outlet, an air bent, and a lid having a cultivation solution inlet and an air inlet.
摘要翻译：目的：提供一种细胞培养器，其向细胞培养液提供充足的空气并防止其中的泡沫产生，由此去除培养细胞的致死因子。所述的耕耘机包括栽培板，栽培板的支架，容纳栽培板的开口杆，空气喷嘴，栽培板的固定槽，栽培容器支架，容纳栽培容器支架的锁钩和固定盖子，具有培养溶液出口的外部容器，弯曲的空气以及具有培养溶液入口和空气入口的盖子。

6. 发明公开

KR1020030043168A 셀룰라제 또는 락타제 조성물 와이-에이오를 이용한진세노사이드 컴파운드 케이의 제조방법 有权
标题翻译：使用纤维素酶或乳酸酶组合物制备GINSENOSIDE化合物K的方法Y-AO
公开(公告)号：KR1020030043168A
公开(公告)日：2003-06-02
申请号：KR1020010074202
申请日：2001-11-27
申请人： 주식회사 케이티앤지
发明人： 고성룡 , 스즈끼유키오 , 최강주 , 조병구 , 양재원 , 박종대 , 김영회 , 박은경
IPC分类号： C12P33/16
摘要： PURPOSE: A method of preparing a ginsenoside compound K is provided to make the ginsenoside product at high yield of 80% or more with effects of immuno-stimulation, cancer inhibition, and vascular tumor inhibition. CONSTITUTION: In a method of preparing a ginsenoside compound K, a diol-based saponin extract from ginseng is dissolved in a water solvent or a mixture of a water solvent and an organic solvent, and cellulase isolated from Aspergillus niger or a lactase composition Y-AO where 78-82 wt.% of dextrin powder is mixed with 18-22 wt.% of lactase powder isolated from Aspergillus oryzae is added to the solution. The solution is stirred in a heated water bath during the enzymatic reaction. The reaction solution is mixed with water-saturated butanol to obtain a butanol layer with an eluate of ginsenoside compound K. The resulting product is concentrated, and subjected to column-chromatography to obtain ginsenoside compound K. The ginseng is selected from Panax ginseng, Panax quinquefolium, Panax japonica, or Panax notoginseng.
摘要翻译：目的：提供一种人参皂苷化合物K的制备方法，以免疫刺激，癌症抑制和血管肿瘤抑制作用，使人参皂苷产物的产率高达80％以上。构成：在制备人参皂苷化合物K的方法中，将来自人参的二醇类皂苷提取物溶解于水溶剂或水溶剂与有机溶剂的混合物中，从黑曲霉或乳糖酶组合物Y- AO，其中将78-82重量％的糊精粉末与18-22重量％的从米曲霉分离的乳糖酶粉末混合加入到溶液中。在酶促反应期间，将溶液在加热的水浴中搅拌。将反应溶液与水饱和的丁醇混合，得到具有人参皂苷化合物K的洗脱液的丁醇层。将所得产物浓缩，并进行柱色谱法以获得人参皂苷化合物K.人参选自人参，人参五味子，三七或三七。

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