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1. 发明公开

KR1020090038731A 도메인 치환법에 의한 글리코실트랜스퍼라아제하이브리드의 제조방법 无效
标题翻译：使用域切换进行糖基转移酶杂交的制备方法
公开(公告)号：KR1020090038731A
公开(公告)日：2009-04-21
申请号：KR1020070104183
申请日：2007-10-16
申请人： 주식회사 진켐
发明人： 우진석 , 송재경 , 김병기 , 이희찬 , 류광경 , 김대희 , 강선엽 , 박성희 , 양지영 , 서원민 , 길태건 , 심상희
IPC分类号： C12N9/10 , C12N9/00
摘要： A method for preparing glycosyltransferase hybrid through domain substitution is provided to generate glycosyltransferase hybrid having C-terminal domain derived from the first glycosyltransferase and N-terminal domain derived from the second glycosyltransferase. Glycosyltransferase hybrid comprises C-terminal domain derived from the first glycosyltransferase and N-terminal domain derived from the second glycosyltransferase. The C-terminal domain derived from the first glycosyltransferase and N-terminal domain derived from the second glycosyltransferase are linked with each other by linker loop. The first glycosyltransferase and the second glycosyltransferase are respectively selected from the group consisting of GtFA, GtFB, GtFC, GtFD, GtFE, KanF, BtrM, GtmG, NemD, RbmD and TbmD. A method for preparing the glycosyltransferase hybrid consists of the following steps of: preparing a recombinant vector having a gene coding glycosyltransferase hybrid; transforming a microorganism with the recombinant vector; and cultivating the transformed microorganism to obtain the glycosyltransferase hybrid.
摘要翻译：提供通过领域取代制备糖基转移酶杂交体的方法，以产生具有衍生自第一糖基转移酶的C-末端结构域和衍生自第二糖基转移酶的N末端结构域的糖基转移酶杂交体。糖基转移酶杂合物包含衍生自第一糖基转移酶的C-末端结构域和衍生自第二糖基转移酶的N-末端结构域。衍生自第一糖基转移酶的C末端结构域和衍生自第二糖基转移酶的N-末端结构域通过接头环相互连接。第一糖基转移酶和第二糖基转移酶分别选自GtFA，GtFB，GtFC，GtFD，GtFE，KanF，BtrM，GtmG，NemD，RbmD和TbmD。制备糖基转移酶杂交体的方法包括以下步骤：制备具有编码糖基转移酶杂交基因的重组载体; 用重组载体转化微生物; 并培养转化的微生物以获得糖基转移酶杂交体。

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