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    • 9. 发明公开
    • 대추씨 추출물을 함유하는 항돌연변이 및 암세포 성장억제활성 조성물
    • 含有ZIZYPHUS JUJUBE SEED提取物的抗生素和抗虫剂组合物
    • KR1020080010193A
    • 2008-01-30
    • KR1020060070374
    • 2006-07-26
    • 박준홍장은영백은정김현정이석일권대준김은정주식회사 정문
    • 박준홍장은영백은정김현정이석일권대준김은정곽희부
    • A61K36/725A61P35/00
    • Y02A50/481A61K36/725A23V2200/308A61K2236/331
    • A composition comprising the extract of Zizyphus jujube seed is provided to inhibit oxidation, mutation induced by mutation-inducing chemicals and growth of liver cancer cells, colon carcinoma cells, blood cancer cells and promyelocytic leukemia cells, and promote growth of normal liver cells. An anti-mutagenic and anti-cancer composition comprises the extract of Zizyphus jujube seed which is prepared by extracting Zizyphus jujube seeds with water at 90-100 deg. C for 8 hours or with 99% methanol for 8 hours, and filtering and concentrating the water or methanol extract under reduced pressure, wherein the anti-oxidant effect is measured by reacting acetic acid with antioxidant solution, and measuring absorbance; the anti-mutagenic effect is determined by treating Salmonella typhimurium TA100 with N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) and 4-nitroquinoline-N-oxide(4NQO); and the anti-cancer effect is determined by measuring cytotoxicity of MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) for cancer cells and normal cells.
    • 提供了包含枣子提取物的组合物,以抑制氧化,突变诱导化学物质引起的突变和肝癌细胞,结肠癌细胞,血液癌细胞和早幼粒细胞白血病细胞的生长,促进正常肝细胞的生长。 抗诱变和抗癌组合物包括通过在90-100度的水提取枣子枣种子制备的枣子枣种子的提取物。 8小时,或用99%甲醇处理8小时,减压过滤浓缩水或甲醇提取物,其中抗氧化作用通过乙酸与抗氧化剂溶液反应并测量吸光度; 通过用N-甲基-N'-硝基-N-亚硝基胍(MNNG)和4-硝基喹啉-N-氧化物(4NQO)处理鼠伤寒沙门氏菌TA100来测定抗诱变作用; 并且通过测量MTT(3- [4,5-二甲基噻唑-2-基] -2,5-二苯基四唑溴化物)对癌细胞和正常细胞的细胞毒性来确定抗癌作用。